MS-Fit Search Results

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Sample ID (comment): T006
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 827 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.93e+007	20/40 (50%)	61055.2 / 5.70	HUMAN	60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (P60 LYMPHOCYTE PROTEIN) (HUCHA60)
2	7.29e+005	17/40 (42%)	60956.0 / 5.91	MOUSE	60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (HSP-65)
3	1.94e+003	7/40 (17%)	57878.3 / 7.58	HUMAN	PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1)
4	1.93e+003	7/40 (17%)	57914.4 / 7.95	HUMAN	PYRUVATE KINASE, M2 ISOZYME
5	247	4/40 (10%)	17318.1 / 5.52	HUMAN	THYROID RECEPTOR INTERACTING PROTEIN 3 (TRIP3)

Detailed Results

1. 20/40 matches (50%). 61055.2 Da, pI = 5.70. Acc. # P10809. HUMAN. 60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (P60 LYMPHOCYTE PROTEIN) (HUCHA60).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
833.3525	833.3906	-45.7135	302	309	(K)(K)
855.4316	855.4688	-43.5311	134	141	(K)(R)
960.4693	960.5114	-43.8554	421	429	(R)(A)
1344.6713	1344.7163	-33.5313	61	72	(R)(V)
1389.6730	1389.7054	-23.3265	222	233	(R)(G)
1504.7057	1504.7569	-34.0362	206	218	(K)(F)
1600.8692	1600.9096	-25.2568	142	156	(R)RGVMLAVDAVIAELK(K)	1Met-ox
1615.7319	1615.7678	-22.2051	237	249	(K)CEFQDAYVLLSEK(K)	1Cys-am
1698.8846	1698.9213	-21.5561	430	446	(R)AAVEEGIVLGGGCALLR(C)	1Cys-am
1702.8398	1702.8804	-23.8856	222	236	(R)(C)
1712.9351	1713.0097	-43.5183	142	157	(R)(Q)
1919.0356	1919.0714	-18.6616	251	268	(K)(K)
1938.9091	1938.9483	-20.1964	206	221	(K)TLNDELEIIEGMKFDR(G)	1Met-ox
1968.1033	1968.1395	-18.3924	291	309	(R)(K)
2038.0069	2038.0232	-8.0111	371	387	(R)(E)
2047.1279	2047.1664	-18.8147	250	268	(K)(K)
2129.0624	2129.1350	-34.0959	38	58	(R)ALMLQGVDLLADAVAVTMGPK(G)	1Met-ox
2295.1424	2295.1608	-8.0034	371	389	(R)(L)
2365.3026	2365.3343	-13.3884	269	290	(R)(L)
2560.2317	2560.2491	-6.7730	97	121	(K)(S)

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The matched peptides cover 40% (231/573AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 50.0 Mean Error = -25.3376 ppm Data Tolerance = 24.7909 ppm Mean Missed Cleavages = 0.5

2. 17/40 matches (42%). 60956.0 Da, pI = 5.91. Acc. # P19226. MOUSE. 60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR (HSP60) (60 KDA CHAPERONIN) (CPN60) (HEAT SHOCK PROTEIN 60) (HSP-60) (MITOCHONDRIAL MATRIX PROTEIN P1) (HSP-65).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
833.3525 833.3906 -45.7135 302 309 (K)(K)
855.4316 855.4688 -43.5311 134 141 (K)(R)
960.4693 960.5114 -43.8554 421 429 (R)(A)
1304.6981 1304.6884 7.4219 181 192 (K)(V)
1344.6713 1344.7163 -33.5313 61 72 (R)(V)
1389.6730 1389.7054 -23.3265 222 233 (R)(G)
1504.7057 1504.7569 -34.0362 206 218 (K)(F)
1600.8692 1600.9096 -25.2568 142 156 (R)RGVMLAVDAVIAELK(K) 1Met-ox
1615.7319 1615.7678 -22.2051 237 249 (K)CEFQDAYVLLSEK(K) 1Cys-am
1698.8846 1698.9213 -21.5561 430 446 (R)AAVEEGIVLGGGCALLR(C) 1Cys-am
1702.8398 1702.8804 -23.8856 222 236 (R)(C)
1712.9351 1713.0097 -43.5183 142 157 (R)(Q)
1938.9091 1938.9483 -20.1964 206 221 (K)TLNDELEIIEGMKFDR(G) 1Met-ox
1968.1033 1968.1395 -18.3924 291 309 (R)(K)
2129.0624 2129.1350 -34.0959 38 58 (R)ALMLQGVDLLADAVAVTMGPK(G) 1Met-ox
2365.3026 2365.3343 -13.3884 269 290 (R)(L)
2560.2317 2560.2491 -6.7730 97 121 (K)(S)

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The matched peptides cover 35% (205/573AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 42.5 Mean Error = -26.2259 ppm Data Tolerance = 28.6848 ppm Mean Missed Cleavages = 0.5

3. 7/40 matches (17%). 57878.3 Da, pI = 7.58. Acc. # P14618. HUMAN. PYRUVATE KINASE, M1 ISOZYME (PYRUVATE KINASE MUSCLE ISOZYME) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1642.7535 1642.7713 -10.8060 476 489 (K)(V)
1698.8846 1698.9154 -18.0992 462 475 (R)QAHLYRGIFPVLCK(D) pyroGlu 1Cys-am
1859.8630 1859.9002 -19.9975 231 246 (K)(K)
1876.8782 1876.9699 -48.8653 320 336 (R)(K)
1931.9484 1931.9867 -19.8072 384 399 (R)(R)
2088.0641 2088.0878 -11.3339 384 400 (R)(L)
2465.2846 2465.2928 -3.3348 93 115 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1642.7535	1642.7713	-10.8060	476	489	(K)(V)
1698.8846	1698.9154	-18.0992	462	475	(R)QAHLYRGIFPVLCK(D)	pyroGlu 1Cys-am
1859.8630	1859.9002	-19.9975	231	246	(K)(K)
1876.8782	1876.9699	-48.8653	320	336	(R)(K)
1931.9484	1931.9867	-19.8072	384	399	(R)(R)
2088.0641	2088.0878	-11.3339	384	400	(R)(L)
2465.2846	2465.2928	-3.3348	93	115	(R)(G)

The matched peptides cover 19% (101/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = -18.8920 ppm Data Tolerance = 29.0473 ppm Mean Missed Cleavages = 0.3

4. 7/40 matches (17%). 57914.4 Da, pI = 7.95. Acc. # P14786. HUMAN. PYRUVATE KINASE, M2 ISOZYME.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1642.7535 1642.7713 -10.8060 476 489 (K)(V)
1698.8846 1698.9154 -18.0992 462 475 (R)QAHLYRGIFPVLCK(D) pyroGlu 1Cys-am
1859.8630 1859.9002 -19.9975 231 246 (K)(K)
1876.8782 1876.9699 -48.8653 320 336 (R)(K)
1931.9484 1931.9867 -19.8072 384 399 (R)(R)
2088.0641 2088.0878 -11.3339 384 400 (R)(L)
2465.2846 2465.2928 -3.3348 93 115 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1642.7535	1642.7713	-10.8060	476	489	(K)(V)
1698.8846	1698.9154	-18.0992	462	475	(R)QAHLYRGIFPVLCK(D)	pyroGlu 1Cys-am
1859.8630	1859.9002	-19.9975	231	246	(K)(K)
1876.8782	1876.9699	-48.8653	320	336	(R)(K)
1931.9484	1931.9867	-19.8072	384	399	(R)(R)
2088.0641	2088.0878	-11.3339	384	400	(R)(L)
2465.2846	2465.2928	-3.3348	93	115	(R)(G)

The matched peptides cover 19% (101/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.5 Mean Error = -18.8920 ppm Data Tolerance = 29.0473 ppm Mean Missed Cleavages = 0.3

5. 4/40 matches (10%). 17318.1 Da, pI = 5.52. Acc. # Q15649. HUMAN. THYROID RECEPTOR INTERACTING PROTEIN 3 (TRIP3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
960.4693 960.5114 -43.8554 91 99 (K)(S)
1389.6730 1389.6684 3.3249 109 120 (R)(A)
2099.9131 2099.8165 46.0163 66 83 (K)(V)
2295.1424 2295.2391 -42.1379 1 18 (-)LKCSTVVCVICLEKPKYR(C) 3Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
960.4693	960.5114	-43.8554	91	99	(K)(S)
1389.6730	1389.6684	3.3249	109	120	(R)(A)
2099.9131	2099.8165	46.0163	66	83	(K)(V)
2295.1424	2295.2391	-42.1379	1	18	(-)LKCSTVVCVICLEKPKYR(C)	3Cys-am

The matched peptides cover 37% (57/152AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.0 Mean Error = -9.1630 ppm Data Tolerance = 85.5696 ppm Mean Missed Cleavages = 0.5