MS-Fit Search Results

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Sample ID (comment): T009
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 93 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.9e+003	8/24 (33%)	17148.9 / 5.83	HUMAN	NUCLEOSIDE DIPHOSPHATE KINASE A (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NM23-H1)
2	1.72e+003	6/24 (25%)	17298.2 / 8.52	HUMAN	NUCLEOSIDE DIPHOSPHATE KINASE B (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF)
3	1.72e+003	6/24 (25%)	17363.2 / 6.97	MOUSE	NUCLEOSIDE DIPHOSPHATE KINASE B (NDK B) (NDP KINASE B) (NM23-M2) (P18)
4	415	6/24 (25%)	17208.0 / 6.84	MOUSE	NUCLEOSIDE DIPHOSPHATE KINASE A (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NDPK-A) (NM23-M1)
5	98.6	4/24 (16%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 8/24 matches (33%). 17148.9 Da, pI = 5.83. Acc. # P15531. HUMAN. NUCLEOSIDE DIPHOSPHATE KINASE A (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NM23-H1).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1065.5199	1065.5151	4.4684	106	114	(R)GDFCIQVGR(N)	1Cys-am
1149.6580	1149.6421	13.8540	57	66	(K)(Y)
1181.5890	1181.5876	1.1569	40	49	(K)(E)
1344.7734	1344.7640	7.0005	7	18	(R)(G)
1785.9063	1785.9169	-5.9511	89	105	(R)(G)
1801.9161	1801.9118	2.3848	89	105	(R)VMLGETNPADSKPGTIR(G)	1Met-ox
2132.1165	2132.0673	23.0975	67	85	(K)YMHSGPVVAMVWEGLNVVK(T)	1Met-ox
2148.1247	2148.0622	29.0764	67	85	(K)YMHSGPVVAMVWEGLNVVK(T)	2Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 50% (77/152AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 33.3 Mean Error = 9.3859 ppm Data Tolerance = 23.6343 ppm Mean Missed Cleavages = 0.0

2. 6/24 matches (25%). 17298.2 Da, pI = 8.52. Acc. # P22392. HUMAN. NUCLEOSIDE DIPHOSPHATE KINASE B (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1065.5199 1065.5151 4.4684 106 114 (R)GDFCIQVGR(N) 1Cys-am
1344.7734 1344.7640 7.0005 7 18 (R)(G)
1785.9063 1785.9169 -5.9511 89 105 (R)(G)
1801.9161 1801.9118 2.3848 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2132.1165 2132.1803 -29.8994 32 49 (K)(Q)
2148.1247 2148.1752 -23.5262 32 49 (K)GFRLVAMKFLRASEEHLK(Q) 1Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 36% (56/152AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = -7.5871 ppm Data Tolerance = 31.1403 ppm Mean Missed Cleavages = 1.0

3. 6/24 matches (25%). 17363.2 Da, pI = 6.97. Acc. # Q01768. MOUSE. NUCLEOSIDE DIPHOSPHATE KINASE B (NDK B) (NDP KINASE B) (NM23-M2) (P18).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1065.5199 1065.5151 4.4684 106 114 (R)GDFCIQVGR(N) 1Cys-am
1344.7734 1344.7640 7.0005 7 18 (R)(G)
1785.9063 1785.9169 -5.9511 89 105 (R)(G)
1801.9161 1801.9118 2.3848 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2132.1165 2132.1803 -29.8994 32 49 (K)(Q)
2148.1247 2148.1752 -23.5262 32 49 (K)GFRLVAMKFLRASEEHLK(Q) 1Met-ox

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 36% (56/152AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = -7.5871 ppm Data Tolerance = 31.1403 ppm Mean Missed Cleavages = 1.0

4. 6/24 matches (25%). 17208.0 Da, pI = 6.84. Acc. # P15532. MOUSE. NUCLEOSIDE DIPHOSPHATE KINASE A (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NDPK-A) (NM23-M1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1065.5199 1065.5151 4.4684 106 114 (R)GDFCIQVGR(N) 1Cys-am
1344.7734 1344.7640 7.0005 7 18 (R)(G)
1785.9063 1785.9169 -5.9511 89 105 (R)(G)
1801.9161 1801.9118 2.3848 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2132.1165 2132.0673 23.0975 67 85 (K)YMHSGPVVAMVWEGLNVVK(T) 1Met-ox
2148.1247 2148.0622 29.0764 67 85 (K)YMHSGPVVAMVWEGLNVVK(T) 2Met-ox

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 37% (57/152AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 10.0128 ppm Data Tolerance = 26.6454 ppm Mean Missed Cleavages = 0.0

5. 4/24 matches (16%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3070 515.3306 -45.6390 404 407 (R)(L)
1475.7754 1475.7858 -7.0726 200 211 (R)(W)
1475.7754 1475.7494 17.5829 212 223 (K)(T)
1993.9919 1993.9772 7.3847 224 239 (R)(R)
2383.9996 2383.9524 19.8074 519 549 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
515.3070	515.3306	-45.6390	404	407	(R)(L)
1475.7754	1475.7858	-7.0726	200	211	(R)(W)
1475.7754	1475.7494	17.5829	212	223	(K)(T)
1993.9919	1993.9772	7.3847	224	239	(R)(R)
2383.9996	2383.9524	19.8074	519	549	(R)(G)

The matched peptides cover 11% (75/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = -1.5873 ppm Data Tolerance = 53.6317 ppm Mean Missed Cleavages = 0.0