MS-Fit Search Results

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Sample ID (comment): T010
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 721 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.37e+004	9/39 (23%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	704	7/39 (17%)	61987.8 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)
3	184	5/39 (12%)	47676.9 / 8.91	HUMAN	PREGNANCY-SPECIFIC BETA-1-GLYCOPROTEIN 10 PRECURSOR (PSBG-10)
4	173	9/39 (23%)	67255.0 / 9.06	MOUSE	ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)
5	143	4/39 (10%)	28940.9 / 8.92	MOUSE	NEUREXOPHILIN 1 PRECURSOR

Detailed Results

1. 9/39 matches (23%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6238	1179.6010	19.3430	377	386	(K)(H)
1300.5563	1300.5302	20.0453	258	267	(K)(N)
1383.7133	1383.6909	16.2055	186	197	(K)(V)
1475.7777	1475.7858	-5.5256	200	211	(R)(W)
1475.7777	1475.7494	19.1299	212	223	(K)(T)
1638.8698	1638.8604	5.7555	186	199	(K)(F)
1716.8492	1716.8517	-1.4278	418	432	(K)(G)
1993.9812	1993.9772	2.0075	224	239	(R)(R)
2150.0447	2150.0783	-15.6153	224	240	(R)(R)
2383.9526	2383.9524	0.0910	519	549	(R)(G)

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The matched peptides cover 18% (121/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.1 Mean Error = 6.0009 ppm Data Tolerance = 24.5674 ppm Mean Missed Cleavages = 0.2

2. 7/39 matches (17%). 61987.8 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1157.5986 1157.5915 6.1543 250 260 (R)(Q)
1307.6938 1307.6782 11.9392 240 249 (R)(Q)
1707.7760 1707.7509 14.7238 46 62 (R)(G)
1791.7336 1791.7283 2.9576 490 512 (R)(G)
1837.9341 1837.9660 -17.3152 374 389 (R)(K)
2299.1474 2299.1202 11.8508 271 289 (K)(N)
2705.1569 2705.1617 -1.7590 63 94 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1157.5986	1157.5915	6.1543	250	260	(R)(Q)
1307.6938	1307.6782	11.9392	240	249	(R)(Q)
1707.7760	1707.7509	14.7238	46	62	(R)(G)
1791.7336	1791.7283	2.9576	490	512	(R)(G)
1837.9341	1837.9660	-17.3152	374	389	(R)(K)
2299.1474	2299.1202	11.8508	271	289	(K)(N)
2705.1569	2705.1617	-1.7590	63	94	(R)(G)

The matched peptides cover 20% (128/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.9 Mean Error = 4.0788 ppm Data Tolerance = 22.1036 ppm Mean Missed Cleavages = 0.4

3. 5/39 matches (12%). 47676.9 Da, pI = 8.91. Acc. # Q15235. HUMAN. PREGNANCY-SPECIFIC BETA-1-GLYCOPROTEIN 10 PRECURSOR (PSBG-10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
807.4078 807.4365 -35.4948 371 377 (K)(L)
1179.6238 1179.5720 43.9219 255 264 (K)(S)
1307.6938 1307.6669 20.5297 254 264 (K)(S)
1837.9341 1837.9093 13.5054 1 16 (-)MGPLSAPPCTQHITWK(G) 1Cys-am
2150.0447 2150.0294 7.1544 127 147 (R)(R)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
807.4078	807.4365	-35.4948	371	377	(K)(L)
1179.6238	1179.5720	43.9219	255	264	(K)(S)
1307.6938	1307.6669	20.5297	254	264	(K)(S)
1837.9341	1837.9093	13.5054	1	16	(-)MGPLSAPPCTQHITWK(G)	1Cys-am
2150.0447	2150.0294	7.1544	127	147	(R)(R)

The matched peptides cover 12% (55/424AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.8 Mean Error = 9.9233 ppm Data Tolerance = 57.8939 ppm Mean Missed Cleavages = 0.2

4. 9/39 matches (23%). 67255.0 Da, pI = 9.06. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN 37 (ZFP-37) (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1157.5986 1157.6101 -9.9367 574 583 (K)(I)
1179.6238 1179.5945 24.8749 406 415 (K)(I)
1267.7153 1267.6534 48.8253 120 130 (K)(S)
1791.7336 1791.7642 -17.0877 33 48 (K)LENCSNPASMGNQDPK(Q) 1Met-ox 1Cys-am
2211.0873 2211.0940 -3.0454 216 234 (K)(R)
2283.1978 2283.1078 39.4150 152 171 (K)(K)
2299.1474 2299.1278 8.5105 81 100 (K)(S)
2370.0147 2370.0495 -14.6833 276 295 (R)THTGEKPYECNECGIAFSQK(S) 1Cys-am
2383.9526 2384.0651 -47.1855 276 295 (R)THTGEKPYECNECGIAFSQK(S) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1157.5986	1157.6101	-9.9367	574	583	(K)(I)
1179.6238	1179.5945	24.8749	406	415	(K)(I)
1267.7153	1267.6534	48.8253	120	130	(K)(S)
1791.7336	1791.7642	-17.0877	33	48	(K)LENCSNPASMGNQDPK(Q)	1Met-ox 1Cys-am
2211.0873	2211.0940	-3.0454	216	234	(K)(R)
2283.1978	2283.1078	39.4150	152	171	(K)(K)
2299.1474	2299.1278	8.5105	81	100	(K)(S)
2370.0147	2370.0495	-14.6833	276	295	(R)THTGEKPYECNECGIAFSQK(S)	1Cys-am
2383.9526	2384.0651	-47.1855	276	295	(R)THTGEKPYECNECGIAFSQK(S)	2Cys-am

The matched peptides cover 21% (126/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.1 Mean Error = 3.2986 ppm Data Tolerance = 60.7157 ppm Mean Missed Cleavages = 1.2

5. 4/39 matches (10%). 28940.9 Da, pI = 8.92. Acc. # Q61200. MOUSE. NEUREXOPHILIN 1 PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1090.5475 1090.5017 42.0680 49 57 (K)(Y)
1566.7496 1566.7527 -2.0063 99 111 (K)(T)
1689.8721 1689.9063 -20.2571 155 169 (K)(D)
1689.8721 1689.7906 48.2007 170 182 (K)DSKSFNCRIEYEK(V) 1Cys-am
2528.2430 2528.1702 28.7742 199 218 (K)TCYQEQTQSHVSWLCSKPFK(V) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1090.5475	1090.5017	42.0680	49	57	(K)(Y)
1566.7496	1566.7527	-2.0063	99	111	(K)(T)
1689.8721	1689.9063	-20.2571	155	169	(K)(D)
1689.8721	1689.7906	48.2007	170	182	(K)DSKSFNCRIEYEK(V)	1Cys-am
2528.2430	2528.1702	28.7742	199	218	(K)TCYQEQTQSHVSWLCSKPFK(V)	1Cys-am

The matched peptides cover 27% (70/253AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 10.3 Mean Error = 19.3559 ppm Data Tolerance = 58.8396 ppm Mean Missed Cleavages = 0.6