MS-Fit Search Results

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Sample ID (comment): T011
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 120000 Da) selects 95950 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10850 entries.
Pre searches select 10850 entries.
MS-Fit search selects 1079 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.69e+003	9/46 (19%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	277	11/46 (23%)	66675.7 / 9.14	HUMAN	PROTEIN PHOSPHATASE 2C DELTA ISOFORM (PP2C-DELTA) (P53-INDUCED PROTEIN PHOSPHATASE 1) (PROTEIN PHOSPHATASE MAGNESIUM-DEPENDENT 1 DELTA)
3	178	9/46 (19%)	102931.6 / 6.32	HUMAN	THYROID PEROXIDASE PRECURSOR (TPO)
4	146	6/46 (13%)	63443.3 / 5.92	HUMAN	M-PHASE INDUCER PHOSPHATASE 2 (DUAL SPECIFICITY PHOSPHATASE CDC25B)
5	117	10/46 (21%)	89614.9 / 8.61	HUMAN	TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20)

Detailed Results

1. 9/46 matches (19%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1066.5528	1066.5169	33.6788	270	277	(K)(T)
1127.5516	1127.5407	9.6539	289	298	(K)(V)
1179.6003	1179.6010	-0.5730	377	386	(K)(H)
1383.7022	1383.6909	8.1784	186	197	(K)(V)
1475.7797	1475.7858	-4.1575	200	211	(R)(W)
1475.7797	1475.7494	20.4980	212	223	(K)(T)
1487.7751	1487.8043	-19.5951	240	251	(R)(L)
1716.8634	1716.8517	6.8164	418	432	(K)(G)
1994.0211	1993.9772	22.0272	224	239	(R)(R)
2384.0202	2383.9524	28.4549	519	549	(R)(G)

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The matched peptides cover 21% (138/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.6 Mean Error = 10.4982 ppm Data Tolerance = 32.3544 ppm Mean Missed Cleavages = 0.5

2. 11/46 matches (23%). 66675.7 Da, pI = 9.14. Acc. # O15297. HUMAN. PROTEIN PHOSPHATASE 2C DELTA ISOFORM (PP2C-DELTA) (P53-INDUCED PROTEIN PHOSPHATASE 1) (PROTEIN PHOSPHATASE MAGNESIUM-DEPENDENT 1 DELTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1128.5660 1128.5326 29.6438 421 429 (K)(V)
1179.6003 1179.6010 -0.5730 127 137 (K)(V)
1307.6835 1307.6959 -9.4884 126 137 (K)(V)
1475.7797 1475.7656 9.5716 144 155 (R)KGFLACHLAMWK(K) 1Cys-am
1475.7797 1475.7656 9.5716 145 156 (K)GFLACHLAMWKK(L) 1Cys-am
1487.7751 1487.7454 19.9622 558 572 (R)(K)
1566.7670 1566.7123 34.8915 96 110 (R)(E)
1593.7910 1593.8059 -9.3287 129 143 (K)(K)
1605.7477 1605.7729 -15.6693 515 528 (K)(T)
1605.7477 1605.8270 -49.3861 536 549 (K)RTLEESNSGPLMKK(H) 1Met-ox
1994.0211 1993.9410 40.1671 337 353 (K)(A)
2161.1328 2161.1366 -1.7155 210 227 (R)(I)
2535.3672 2535.2747 36.4961 336 357 (K)KYLMGEHGQSCAKMLVNRALGR(W) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1128.5660	1128.5326	29.6438	421	429	(K)(V)
1179.6003	1179.6010	-0.5730	127	137	(K)(V)
1307.6835	1307.6959	-9.4884	126	137	(K)(V)
1475.7797	1475.7656	9.5716	144	155	(R)KGFLACHLAMWK(K)	1Cys-am
1475.7797	1475.7656	9.5716	145	156	(K)GFLACHLAMWKK(L)	1Cys-am
1487.7751	1487.7454	19.9622	558	572	(R)(K)
1566.7670	1566.7123	34.8915	96	110	(R)(E)
1593.7910	1593.8059	-9.3287	129	143	(K)(K)
1605.7477	1605.7729	-15.6693	515	528	(K)(T)
1605.7477	1605.8270	-49.3861	536	549	(K)RTLEESNSGPLMKK(H)	1Met-ox
1994.0211	1993.9410	40.1671	337	353	(K)(A)
2161.1328	2161.1366	-1.7155	210	227	(R)(I)
2535.3672	2535.2747	36.4961	336	357	(K)KYLMGEHGQSCAKMLVNRALGR(W)	1Met-ox

The matched peptides cover 22% (138/605AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.9 Mean Error = 7.2418 ppm Data Tolerance = 51.0109 ppm Mean Missed Cleavages = 1.2

3. 9/46 matches (19%). 102931.6 Da, pI = 6.32. Acc. # P07202. HUMAN. THYROID PEROXIDASE PRECURSOR (TPO).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1066.5528 1066.5832 -28.4336 762 769 (R)RVLVYSCR(H) 1Cys-am
1174.6268 1174.6003 22.5494 837 846 (R)TCVDSGRLPR(A) 1Cys-am
1335.7060 1335.6592 35.0507 151 161 (K)YRPITGACNNR(D) 1Cys-am
1593.7910 1593.7695 13.5010 371 386 (R)(G)
1593.7910 1593.8497 -36.7959 649 662 (R)TGPLFACLIGKQMK(A) 1Met-ox 1Cys-am
1636.7609 1636.6873 44.9535 909 924 (R)(D)
2071.1191 2071.0792 19.2522 48 64 (K)RLVDTAMYATMQRNLKK(R) 2Met-ox
2071.1191 2071.0792 19.2522 49 65 (R)LVDTAMYATMQRNLKKR(G) 2Met-ox
2161.1328 2161.1837 -23.5321 90 108 (R)(T)
2161.1328 2161.2102 -35.7886 647 665 (R)ARTGPLFACLIGKQMKALR(D) 1Met-ox 1Cys-am
2211.1596 2211.1006 26.6891 207 225 (R)(Y)
2717.1045 2717.1870 -30.3693 814 836 (R)CRNTKGGFQCLCADPYELGDDGR(T) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1066.5528	1066.5832	-28.4336	762	769	(R)RVLVYSCR(H)	1Cys-am
1174.6268	1174.6003	22.5494	837	846	(R)TCVDSGRLPR(A)	1Cys-am
1335.7060	1335.6592	35.0507	151	161	(K)YRPITGACNNR(D)	1Cys-am
1593.7910	1593.7695	13.5010	371	386	(R)(G)
1593.7910	1593.8497	-36.7959	649	662	(R)TGPLFACLIGKQMK(A)	1Met-ox 1Cys-am
1636.7609	1636.6873	44.9535	909	924	(R)(D)
2071.1191	2071.0792	19.2522	48	64	(K)RLVDTAMYATMQRNLKK(R)	2Met-ox
2071.1191	2071.0792	19.2522	49	65	(R)LVDTAMYATMQRNLKKR(G)	2Met-ox
2161.1328	2161.1837	-23.5321	90	108	(R)(T)
2161.1328	2161.2102	-35.7886	647	665	(R)ARTGPLFACLIGKQMKALR(D)	1Met-ox 1Cys-am
2211.1596	2211.1006	26.6891	207	225	(R)(Y)
2717.1045	2717.1870	-30.3693	814	836	(R)CRNTKGGFQCLCADPYELGDDGR(T)	2Cys-am

The matched peptides cover 17% (159/933AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 19.6 Mean Error = 2.1940 ppm Data Tolerance = 61.0670 ppm Mean Missed Cleavages = 1.5

4. 6/46 matches (13%). 63443.3 Da, pI = 5.92. Acc. # P30305. HUMAN. M-PHASE INDUCER PHOSPHATASE 2 (DUAL SPECIFICITY PHOSPHATASE CDC25B).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1355.5927 1355.6272 -25.4632 428 438 (R)(T)
1636.7609 1636.8157 -33.5166 467 479 (R)(G)
1697.8392 1697.7870 30.7245 339 353 (R)(A)
1740.9443 1740.9617 -9.9963 307 321 (R)(L)
1946.9521 1946.9911 -19.9971 467 482 (R)(M)
2161.1328 2161.1327 0.0613 448 466 (R)DAESFLLKSPIAPCSLDKR(V) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1355.5927	1355.6272	-25.4632	428	438	(R)(T)
1636.7609	1636.8157	-33.5166	467	479	(R)(G)
1697.8392	1697.7870	30.7245	339	353	(R)(A)
1740.9443	1740.9617	-9.9963	307	321	(R)(L)
1946.9521	1946.9911	-19.9971	467	482	(R)(M)
2161.1328	2161.1327	0.0613	448	466	(R)DAESFLLKSPIAPCSLDKR(V)	1Cys-am

The matched peptides cover 13% (76/566AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.0 Mean Error = -9.6979 ppm Data Tolerance = 46.0543 ppm Mean Missed Cleavages = 0.7

5. 10/46 matches (21%). 89614.9 Da, pI = 8.61. Acc. # P21580. HUMAN. TUMOR NECROSIS FACTOR, ALPHA-INDUCED PROTEIN 3 (PUTATIVE DNA BINDING PROTEIN A20) (ZINC FINGER PROTEIN A20).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6003 1179.5581 35.8056 677 685 (K)CFIEAQNQR(F) 1Cys-am
1335.7060 1335.6439 46.4734 597 608 (R)(K)
1593.7910 1593.7776 8.4318 716 728 (K)CARASCKNILACR(S) 1Cys-am
1636.7609 1636.7866 -15.6927 750 763 (R)(A)
1716.8634 1716.8267 21.3603 288 301 (K)(L)
1791.7717 1791.8601 -49.2951 677 690 (K)CFIEAQNQRFHEAK(R) 1Cys-am
1874.0404 1873.9667 39.3618 703 718 (R)DVPRTTQSTSRPKCAR(A) 1Cys-am
1994.0211 1994.0500 -14.4676 25 41 (R)(T)
2071.1191 2071.0898 14.1592 288 304 (K)(E)
2535.3672 2535.2931 29.2342 25 45 (R)TPEDIFKPTNGIIHHFKTMHR(Y) 1Met-ox

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.6003	1179.5581	35.8056	677	685	(K)CFIEAQNQR(F)	1Cys-am
1335.7060	1335.6439	46.4734	597	608	(R)(K)
1593.7910	1593.7776	8.4318	716	728	(K)CARASCKNILACR(S)	1Cys-am
1636.7609	1636.7866	-15.6927	750	763	(R)(A)
1716.8634	1716.8267	21.3603	288	301	(K)(L)
1791.7717	1791.8601	-49.2951	677	690	(K)CFIEAQNQRFHEAK(R)	1Cys-am
1874.0404	1873.9667	39.3618	703	718	(R)DVPRTTQSTSRPKCAR(A)	1Cys-am
1994.0211	1994.0500	-14.4676	25	41	(R)(T)
2071.1191	2071.0898	14.1592	288	304	(K)(E)
2535.3672	2535.2931	29.2342	25	45	(R)TPEDIFKPTNGIIHHFKTMHR(Y)	1Met-ox

The matched peptides cover 13% (104/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 21.7 Mean Error = 11.5371 ppm Data Tolerance = 60.1043 ppm Mean Missed Cleavages = 1.3