MS-Fit Search Results

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Sample ID (comment): T012
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 1141 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.82e+006	14/44 (31%)	49229.9 / 5.89	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H)
2	4.44e+004	10/44 (22%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
3	2.71e+004	12/44 (27%)	59519.1 / 5.13	HUMAN	KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10)
4	4.48e+003	10/44 (22%)	49264.0 / 5.89	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H' (HNRNP H') (FTP-3)
5	341	7/44 (15%)	71945.9 / 6.34	HUMAN	KININOGEN PRECURSOR (ALPHA-2-THIOL PROTEINASE INHIBITOR) [CONTAINS: BRADYKININ]

Detailed Results

1. 14/44 matches (31%). 49229.9 Da, pI = 5.89. Acc. # P31943. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H (HNRNP H).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
605.3541	605.3411	21.5187	295	299	(R)(A)
699.3838	699.3830	1.1815	45	49	(R)(E)
1092.6001	1092.5802	18.2569	317	326	(R)(V)
1365.6893	1365.6724	12.3754	1	14	(-)MLGTEGGEGFVVK(V)	Acet N
1383.7118	1383.6769	25.2076	218	230	(R)(M)
1518.7444	1518.7011	28.4857	17	29	(R)GLPWSCSADEVQR(F)	1Cys-am
1615.7175	1615.6633	33.4990	263	275	(R)DLNYCFSGMSDHR(Y)	1Cys-am
1631.7182	1631.6583	36.7512	263	275	(R)DLNYCFSGMSDHR(Y)	1Met-ox 1Cys-am
1684.8099	1684.7679	24.9372	99	114	(K)(L)
1841.9024	1841.8921	5.5887	151	167	(R)(A)
1997.0504	1996.9769	36.8413	300	316	(R)(V)
2107.0621	2106.9831	37.4583	50	68	(R)(L)
2142.0932	2142.0256	31.5594	356	375	(R)(Y)
2717.1583	2717.0453	41.6032	234	259	(R)(F)

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The matched peptides cover 41% (188/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.8 Mean Error = 25.3760 ppm Data Tolerance = 24.7805 ppm Mean Missed Cleavages = 0.1

2. 10/44 matches (22%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6244 1179.6010 19.8678 377 386 (K)(H)
1277.7548 1277.7105 34.6155 473 483 (K)(T)
1383.7118 1383.6909 15.1482 186 197 (K)(V)
1475.8051 1475.7858 13.0557 200 211 (R)(W)
1475.8051 1475.7494 37.7116 212 223 (K)(T)
1487.8005 1487.8043 -2.5229 240 251 (R)(L)
1657.8300 1657.7934 22.0340 13 29 (R)(R)
1716.8888 1716.8517 21.6569 418 432 (K)(G)
1994.0642 1993.9772 43.6494 224 239 (R)(R)
2121.0520 2121.0114 19.1721 9 29 (R)(R)
2384.0187 2383.9524 27.8089 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6244	1179.6010	19.8678	377	386	(K)(H)
1277.7548	1277.7105	34.6155	473	483	(K)(T)
1383.7118	1383.6909	15.1482	186	197	(K)(V)
1475.8051	1475.7858	13.0557	200	211	(R)(W)
1475.8051	1475.7494	37.7116	212	223	(K)(T)
1487.8005	1487.8043	-2.5229	240	251	(R)(L)
1657.8300	1657.7934	22.0340	13	29	(R)(R)
1716.8888	1716.8517	21.6569	418	432	(K)(G)
1994.0642	1993.9772	43.6494	224	239	(R)(R)
2121.0520	2121.0114	19.1721	9	29	(R)(R)
2384.0187	2383.9524	27.8089	519	549	(R)(G)

The matched peptides cover 23% (152/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.7 Mean Error = 22.9270 ppm Data Tolerance = 25.6123 ppm Mean Missed Cleavages = 0.4

3. 12/44 matches (27%). 59519.1 Da, pI = 5.13. Acc. # P13645. HUMAN. KERATIN, TYPE I CYTOSKELETAL 10 (CYTOKERATIN 10) (K10) (CK 10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1090.5587 1090.5315 24.9281 148 156 (K)(L)
1109.5280 1109.4904 33.8851 335 343 (K)(S)
1165.6093 1165.5853 20.6044 442 450 (R)(S)
1300.6471 1300.5956 39.6480 286 295 (K)(N)
1357.7683 1357.7188 36.4504 246 257 (R)(V)
1365.6893 1365.6399 36.2075 323 333 (R)(K)
1434.7958 1434.7705 17.6587 440 450 (K)(S)
1493.7766 1493.7348 27.9604 323 334 (R)(D)
1707.8295 1707.7727 33.2687 41 59 (K)(G)
1878.0197 1877.9973 11.9394 164 179 (K)(E)
1997.0504 1996.9715 39.5255 346 362 (K)(S)
2872.4556 2872.3933 21.6925 296 322 (R)(S)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1090.5587	1090.5315	24.9281	148	156	(K)(L)
1109.5280	1109.4904	33.8851	335	343	(K)(S)
1165.6093	1165.5853	20.6044	442	450	(R)(S)
1300.6471	1300.5956	39.6480	286	295	(K)(N)
1357.7683	1357.7188	36.4504	246	257	(R)(V)
1365.6893	1365.6399	36.2075	323	333	(R)(K)
1434.7958	1434.7705	17.6587	440	450	(K)(S)
1493.7766	1493.7348	27.9604	323	334	(R)(D)
1707.8295	1707.7727	33.2687	41	59	(K)(G)
1878.0197	1877.9973	11.9394	164	179	(K)(E)
1997.0504	1996.9715	39.5255	346	362	(K)(S)
2872.4556	2872.3933	21.6925	296	322	(R)(S)

The matched peptides cover 23% (142/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 27.3 Mean Error = 28.6474 ppm Data Tolerance = 18.4238 ppm Mean Missed Cleavages = 0.5

4. 10/44 matches (22%). 49264.0 Da, pI = 5.89. Acc. # P55795. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H' (HNRNP H') (FTP-3).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
605.3541 605.3411 21.5187 295 299 (R)(A)
699.3838 699.3830 1.1815 45 49 (R)(E)
1092.6001 1092.5802 18.2569 317 326 (R)(V)
1383.7118 1383.6769 25.2076 218 230 (R)(M)
1521.7424 1521.6830 39.0168 17 29 (R)GLPWSCSADEVMR(F) 1Cys-am
1615.7175 1615.7824 -40.1845 1 14 (-)MMLSTEGREGFVVK(V) 2Met-ox
1615.7175 1615.6633 33.4990 263 275 (R)DLNYCFSGMSDHR(Y) 1Cys-am
1631.7182 1631.6583 36.7512 263 275 (R)DLNYCFSGMSDHR(Y) 1Met-ox 1Cys-am
1684.8099 1684.7679 24.9372 99 114 (K)(L)
1841.9024 1841.8921 5.5887 151 167 (R)(A)
2121.0520 2120.9988 25.0990 50 68 (R)(L)

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The matched peptides cover 27% (125/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.7 Mean Error = 17.3520 ppm Data Tolerance = 44.7847 ppm Mean Missed Cleavages = 0.2

5. 7/44 matches (15%). 71945.9 Da, pI = 6.34. Acc. # P01042. HUMAN. KININOGEN PRECURSOR (ALPHA-2-THIOL PROTEINASE INHIBITOR) [CONTAINS: BRADYKININ].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1165.6093 1165.5523 48.9303 102 113 (K)(R)
1179.6244 1179.5680 47.8572 102 113 (K)AATGECTATVGK(R) 1Cys-am
1383.7118 1383.6619 36.1016 209 219 (K)(S)
1716.8888 1716.8907 -1.0942 436 449 (K)QRKHNLGHGHKHER(D) pyroGlu
1997.0504 1996.9910 29.7628 364 380 (K)IYPTVNCQPLGMISLMK(R) 2Met-ox
2107.0621 2107.0170 21.3766 345 362 (K)(K)
2107.0621 2107.1118 -23.6000 363 380 (K)KIYPTVNCQPLGMISLMK(R) 1Cys-am
2121.0520 2121.0327 9.1238 345 362 (K)LGQSLDCNAEVYVVPWEK(K) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1165.6093	1165.5523	48.9303	102	113	(K)(R)
1179.6244	1179.5680	47.8572	102	113	(K)AATGECTATVGK(R)	1Cys-am
1383.7118	1383.6619	36.1016	209	219	(K)(S)
1716.8888	1716.8907	-1.0942	436	449	(K)QRKHNLGHGHKHER(D)	pyroGlu
1997.0504	1996.9910	29.7628	364	380	(K)IYPTVNCQPLGMISLMK(R)	2Met-ox
2107.0621	2107.0170	21.3766	345	362	(K)(K)
2107.0621	2107.1118	-23.6000	363	380	(K)KIYPTVNCQPLGMISLMK(R)	1Cys-am
2121.0520	2121.0327	9.1238	345	362	(K)LGQSLDCNAEVYVVPWEK(K)	1Cys-am

The matched peptides cover 11% (73/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.9 Mean Error = 21.0573 ppm Data Tolerance = 50.2834 ppm Mean Missed Cleavages = 0.5