MS-Fit Search Results

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Sample ID (comment): T013
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 629 entries (results displayed for top 5 matches).

Parameters Used in Search

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.47e+007	17/34 (50%)	26669.7 / 6.45	HUMAN	TRIOSEPHOSPHATE ISOMERASE (TIM)
2	4.4e+004	11/34 (32%)	26712.9 / 6.90	MOUSE	TRIOSEPHOSPHATE ISOMERASE (TIM)
3	242	7/34 (20%)	40297.9 / 9.18	HUMAN	ZINC FINGER PROTEIN 134
4	234	6/34 (17%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
5	173	5/34 (14%)	56779.5 / 9.53	HUMAN	ALPHA-1B ADRENERGIC RECEPTOR (ALPHA 1B-ADRENOCEPTOR)

Detailed Results

1. 17/34 matches (50%). 26669.7 Da, pI = 6.45. Acc. # P00938. HUMAN. TRIOSEPHOSPHATE ISOMERASE (TIM).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
954.4680	954.4838	-16.5098	7	14	(K)(M)
1082.5749	1082.5787	-3.5613	6	14	(R)(M)
1151.5801	1151.5883	-7.0830	60	69	(K)IAVAAQNCYK(V)	1Cys-am
1234.6092	1234.6028	5.2050	195	206	(K)(I)
1340.6947	1340.6884	4.6806	207	219	(R)IIYGGSVTGATCK(E)	1Cys-am
1458.7245	1458.7229	1.0859	101	113	(R)(V)
1466.7130	1466.7239	-7.4940	176	188	(K)(L)
1542.9003	1542.8855	9.5609	19	33	(R)(V)
1600.7663	1600.7542	7.5840	86	99	(K)DCGATWVVLGHSER(R)	1Cys-am
1602.8279	1602.8896	-38.4364	161	175	(K)(T)
1614.8200	1614.8240	-2.4696	100	113	(R)(V)
1621.8240	1621.8260	-1.2399	70	85	(K)(D)
1637.8240	1637.8209	1.9004	70	85	(K)VTNGAFTGEISPGMIK(D)	1Met-ox
1821.9697	1821.9897	-10.9535	114	131	(K)VAHALAEGLGVIACIGEK(L)	1Cys-am
1993.9905	1994.0183	-13.9201	1	18	(-)APSRKFFVGGNWKMNGR(K)	Acet N
2206.1054	2206.0854	9.0314	34	53	(K)VPADTEVVCAPPTAYIDFAR(Q)	1Cys-am
3029.5741	3029.5835	-3.1280	220	248	(K)(Q)

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The matched peptides cover 83% (207/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 50.0 Mean Error = -3.8675 ppm Data Tolerance = 23.6121 ppm Mean Missed Cleavages = 0.4

2. 11/34 matches (32%). 26712.9 Da, pI = 6.90. Acc. # P17751. MOUSE. TRIOSEPHOSPHATE ISOMERASE (TIM).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
954.4680 954.4838 -16.5098 7 14 (K)(M)
1082.5749 1082.5787 -3.5613 6 14 (R)(M)
1151.5801 1151.5883 -7.0830 60 69 (K)IAVAAQNCYK(V) 1Cys-am
1340.6947 1340.6884 4.6806 207 219 (R)IIYGGSVTGATCK(E) 1Cys-am
1458.7245 1458.7229 1.0859 101 113 (R)(V)
1466.7130 1466.7239 -7.4940 176 188 (K)(L)
1602.8279 1602.8896 -38.4364 161 175 (K)(T)
1614.8200 1614.8240 -2.4696 100 113 (R)(V)
1621.8240 1621.8260 -1.2399 70 85 (K)(D)
1637.8240 1637.8209 1.9004 70 85 (K)VTNGAFTGEISPGMIK(D) 1Met-ox
3029.5741 3029.5835 -3.1280 220 248 (K)(Q)

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The matched peptides cover 47% (119/249AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.4 Mean Error = -6.5686 ppm Data Tolerance = 23.9871 ppm Mean Missed Cleavages = 0.2

3. 7/34 matches (20%). 40297.9 Da, pI = 9.18. Acc. # P52741. HUMAN. ZINC FINGER PROTEIN 134.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1041.5989 1041.5594 37.9701 202 210 (R)(C)
1179.5938 1179.5832 8.9512 20 28 (K)CYSIEQPLR(R) 1Cys-am
1602.8279 1602.8539 -16.1645 298 310 (K)CGKDFIRTSHLVR(H) 1Cys-am
1614.8200 1614.8869 -41.4042 245 257 (K)(I)
1637.8240 1637.8301 -3.7168 189 201 (K)(V)
1821.9697 1821.9493 11.2249 29 44 (R)(E)
1993.9905 1993.9046 43.0770 173 188 (K)RIHTGEMPYKCNECGK(Y) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1041.5989	1041.5594	37.9701	202	210	(R)(C)
1179.5938	1179.5832	8.9512	20	28	(K)CYSIEQPLR(R)	1Cys-am
1602.8279	1602.8539	-16.1645	298	310	(K)CGKDFIRTSHLVR(H)	1Cys-am
1614.8200	1614.8869	-41.4042	245	257	(K)(I)
1637.8240	1637.8301	-3.7168	189	201	(K)(V)
1821.9697	1821.9493	11.2249	29	44	(R)(E)
1993.9905	1993.9046	43.0770	173	188	(K)RIHTGEMPYKCNECGK(Y)	1Cys-am

The matched peptides cover 25% (89/348AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.6 Mean Error = 5.7054 ppm Data Tolerance = 59.2454 ppm Mean Missed Cleavages = 1.3

4. 6/34 matches (17%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5938 1179.6010 -6.1037 377 386 (K)(H)
1340.6947 1340.6698 18.5745 365 376 (K)(Y)
1475.7658 1475.7858 -13.5857 200 211 (R)(W)
1475.7658 1475.7494 11.0696 212 223 (K)(T)
1487.7778 1487.8043 -17.7689 240 251 (R)(L)
1993.9905 1993.9772 6.6886 224 239 (R)(R)
2383.9825 2383.9524 12.6261 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5938	1179.6010	-6.1037	377	386	(K)(H)
1340.6947	1340.6698	18.5745	365	376	(K)(Y)
1475.7658	1475.7858	-13.5857	200	211	(R)(W)
1475.7658	1475.7494	11.0696	212	223	(K)(T)
1487.7778	1487.8043	-17.7689	240	251	(R)(L)
1993.9905	1993.9772	6.6886	224	239	(R)(R)
2383.9825	2383.9524	12.6261	519	549	(R)(G)

The matched peptides cover 16% (105/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 17.6 Mean Error = 1.6429 ppm Data Tolerance = 28.1705 ppm Mean Missed Cleavages = 0.6

5. 5/34 matches (14%). 56779.5 Da, pI = 9.53. Acc. # P35368. HUMAN. ALPHA-1B ADRENERGIC RECEPTOR (ALPHA 1B-ADRENOCEPTOR).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6816 1307.6629 14.2772 244 254 (K)(I)
1340.6947 1340.7214 -19.9440 149 159 (R)(R)
1542.9003 1542.8644 23.2559 448 463 (K)(R)
1600.7663 1600.7695 -1.9466 380 392 (R)(G)
1614.8200 1614.7851 21.6127 380 392 (R)LGGCAYTYRPWTR(G) 1Cys-am

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6816	1307.6629	14.2772	244	254	(K)(I)
1340.6947	1340.7214	-19.9440	149	159	(R)(R)
1542.9003	1542.8644	23.2559	448	463	(K)(R)
1600.7663	1600.7695	-1.9466	380	392	(R)(G)
1614.8200	1614.7851	21.6127	380	392	(R)LGGCAYTYRPWTR(G)	1Cys-am

The matched peptides cover 9% (51/519AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.7 Mean Error = 7.4511 ppm Data Tolerance = 36.5479 ppm Mean Missed Cleavages = 0.2