MS-Fit Search Results

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Sample ID (comment): T014
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 346 entries (results displayed for top 5 matches).

Parameters Used in Search

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.72e+005	13/40 (32%)	22327.3 / 7.83	HUMAN	HEAT SHOCK 27 KDA PROTEIN (HSP 27) (STRESS-RESPONSIVE PROTEIN 27) (SRP27) (ESTROGEN-REGULATED 24 KDA PROTEIN) (28 KDA HEAT SHOCK PROTEIN)
2	577	9/40 (22%)	91922.6 / 8.97	HUMAN	ZINC FINGER PROTEIN 226
3	313	6/40 (15%)	23014.0 / 6.12	MOUSE	HEAT SHOCK 27 KD PROTEIN (HSP 27) (GROWTH-RELATED 25 KD PROTEIN) (P25) (HSP25)
4	243	5/40 (12%)	55250.0 / 9.43	HUMAN	ZINC FINGER PROTEIN 141
5	190	6/40 (15%)	62496.2 / 6.08	HUMAN	TRANSDUCIN BETA-LIKE 1 PROTEIN

Detailed Results

1. 13/40 matches (32%). 22327.3 Da, pI = 7.83. Acc. # P04792. HUMAN. HEAT SHOCK 27 KDA PROTEIN (HSP 27) (STRESS-RESPONSIVE PROTEIN 27) (SRP27) (ESTROGEN-REGULATED 24 KDA PROTEIN) (28 KDA HEAT SHOCK PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
599.2753	599.2901	-24.8476	90	94	(R)(W)
831.4584	831.5092	-61.1692	6	12	(R)(G)
960.3809	960.4328	-54.0729	21	27	(R)(L)
961.3958	961.4532	-59.7112	13	20	(R)(D)
987.5495	987.6104	-61.6689	5	12	(R)(G)
1058.5021	1058.5482	-43.6022	80	89	(R)QLSSGVSEIR(H)	pyroGlu
1075.5319	1075.5748	-39.8960	80	89	(R)(H)
1087.4325	1087.4809	-44.5146	128	136	(R)QDEHGYISR(C)	pyroGlu
1104.4667	1104.5074	-36.8734	128	136	(R)(C)
1163.5699	1163.6213	-44.2158	28	37	(R)(L)
1783.8368	1783.9230	-48.3565	97	112	(R)(T)
1902.7909	1902.8676	-40.2977	13	27	(R)(L)
1905.9164	1905.9922	-39.7759	172	188	(K)(A)

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The matched peptides cover 45% (90/199AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.5 Mean Error = -46.0771 ppm Data Tolerance = 21.4836 ppm Mean Missed Cleavages = 0.2

2. 9/40 matches (22%). 91922.6 Da, pI = 8.97. Acc. # Q9NYT6. HUMAN. ZINC FINGER PROTEIN 226.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
599.2753 599.3088 -55.9275 604 607 (K)IHCR(I) 1Cys-am
831.4584 831.4576 0.9367 187 193 (R)(N)
1058.5021 1058.5635 -58.0137 412 420 (R)(C)
1058.5021 1058.5635 -58.0137 440 448 (R)(C)
1058.5021 1058.5635 -58.0137 496 504 (R)(C)
1058.5021 1058.5635 -58.0137 692 700 (R)(C)
1058.5021 1058.5635 -58.0137 748 756 (R)(C)
1087.4325 1087.4916 -54.4125 474 482 (K)(G)
1185.5769 1185.5864 -8.0032 75 85 (R)QGNLGEKNQSK(L) pyroGlu
1185.5769 1185.5145 52.6473 505 513 (K)CNECGKSFR(R) 2Cys-am
1463.6505 1463.7317 -55.4750 62 73 (R)(R)
1466.7691 1466.7617 5.0700 651 663 (K)(V)
1877.9303 1877.8274 54.7728 356 370 (K)VHTAEKPYNCEECGR(A) 2Cys-am
1905.9164 1905.9526 -19.0313 187 201 (R)DQQISIKNELCQCKK(G) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
599.2753	599.3088	-55.9275	604	607	(K)IHCR(I)	1Cys-am
831.4584	831.4576	0.9367	187	193	(R)(N)
1058.5021	1058.5635	-58.0137	412	420	(R)(C)
1058.5021	1058.5635	-58.0137	440	448	(R)(C)
1058.5021	1058.5635	-58.0137	496	504	(R)(C)
1058.5021	1058.5635	-58.0137	692	700	(R)(C)
1058.5021	1058.5635	-58.0137	748	756	(R)(C)
1087.4325	1087.4916	-54.4125	474	482	(K)(G)
1185.5769	1185.5864	-8.0032	75	85	(R)QGNLGEKNQSK(L)	pyroGlu
1185.5769	1185.5145	52.6473	505	513	(K)CNECGKSFR(R)	2Cys-am
1463.6505	1463.7317	-55.4750	62	73	(R)(R)
1466.7691	1466.7617	5.0700	651	663	(K)(V)
1877.9303	1877.8274	54.7728	356	370	(K)VHTAEKPYNCEECGR(A)	2Cys-am
1905.9164	1905.9526	-19.0313	187	201	(R)DQQISIKNELCQCKK(G)	1Cys-am

The matched peptides cover 16% (133/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = -26.3922 ppm Data Tolerance = 83.1439 ppm Mean Missed Cleavages = 0.4

3. 6/40 matches (15%). 23014.0 Da, pI = 6.12. Acc. # P14602. MOUSE. HEAT SHOCK 27 KD PROTEIN (HSP 27) (GROWTH-RELATED 25 KD PROTEIN) (P25) (HSP25).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
831.4584 831.5092 -61.1692 6 12 (R)(S)
987.5495 987.6104 -61.6689 5 12 (R)(S)
1058.5021 1058.5482 -43.6022 84 93 (R)QLSSGVSEIR(Q) pyroGlu
1075.5319 1075.5748 -39.8960 84 93 (R)(Q)
1087.4325 1087.4809 -44.5146 132 140 (R)QDEHGYISR(C) pyroGlu
1104.4667 1104.5074 -36.8734 132 140 (R)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
831.4584	831.5092	-61.1692	6	12	(R)(S)
987.5495	987.6104	-61.6689	5	12	(R)(S)
1058.5021	1058.5482	-43.6022	84	93	(R)QLSSGVSEIR(Q)	pyroGlu
1075.5319	1075.5748	-39.8960	84	93	(R)(Q)
1087.4325	1087.4809	-44.5146	132	140	(R)QDEHGYISR(C)	pyroGlu
1104.4667	1104.5074	-36.8734	132	140	(R)(C)

The matched peptides cover 12% (27/209AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = -47.9541 ppm Data Tolerance = 21.5633 ppm Mean Missed Cleavages = 0.2

4. 5/40 matches (12%). 55250.0 Da, pI = 9.43. Acc. # Q15928. HUMAN. ZINC FINGER PROTEIN 141.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
571.2669 571.2840 -29.9009 167 171 (R)(H)
1058.5021 1058.5635 -58.0137 416 424 (K)(C)
1185.5769 1185.6632 -72.7713 247 256 (K)(C)
1185.5769 1185.5396 31.4304 425 433 (K)(Q)
1185.5769 1185.5396 31.4304 453 461 (K)CKDCDKAFK(R) 1Cys-am
1877.9303 1877.8526 41.3780 191 206 (K)(A)
1905.9164 1905.8839 17.0396 191 206 (K)VIHAGEKPYTCEECGK(A) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
571.2669	571.2840	-29.9009	167	171	(R)(H)
1058.5021	1058.5635	-58.0137	416	424	(K)(C)
1185.5769	1185.6632	-72.7713	247	256	(K)(C)
1185.5769	1185.5396	31.4304	425	433	(K)(Q)
1185.5769	1185.5396	31.4304	453	461	(K)CKDCDKAFK(R)	1Cys-am
1877.9303	1877.8526	41.3780	191	206	(K)(A)
1905.9164	1905.8839	17.0396	191	206	(K)VIHAGEKPYTCEECGK(A)	2Cys-am

The matched peptides cover 12% (58/474AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = -5.6296 ppm Data Tolerance = 94.2044 ppm Mean Missed Cleavages = 0.6

5. 6/40 matches (15%). 62496.2 Da, pI = 6.08. Acc. # O60907. HUMAN. TRANSDUCIN BETA-LIKE 1 PROTEIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
599.2753 599.3088 -55.9275 276 279 (R)HCIR(E) 1Cys-am
831.4584 831.4365 26.3498 489 494 (R)(G)
987.5495 987.4787 71.6152 519 527 (K)(C)
1466.7691 1466.6851 57.3043 545 557 (R)(G)
1591.7323 1591.7651 -20.5859 443 455 (K)(E)
1905.9164 1905.9340 -9.2583 558 576 (R)(K)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
599.2753	599.3088	-55.9275	276	279	(R)HCIR(E)	1Cys-am
831.4584	831.4365	26.3498	489	494	(R)(G)
987.5495	987.4787	71.6152	519	527	(K)(C)
1466.7691	1466.6851	57.3043	545	557	(R)(G)
1591.7323	1591.7651	-20.5859	443	455	(K)(E)
1905.9164	1905.9340	-9.2583	558	576	(R)(K)

The matched peptides cover 11% (64/577AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = 11.5829 ppm Data Tolerance = 97.7312 ppm Mean Missed Cleavages = 0.2