MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): T85
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1337 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/94(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.399e+01323 (24)50.024.526.332.5 52619/5.9HUMANUbiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
22.109e+00510 (10)13.010.628.019.3 52769/5.8MOUSEUbiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
36.902e+00410 (10)24.010.632.417.8 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
43.558e+00410 (10)16.010.61.0067.3 99647/5.4MOUSERod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
52.959e+0047 (7)10.07.42.6951.6 70042/8.0MOUSETyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
51282x..x......x..x.x..xx....x........x......x.x..........x.x.x.........x....x.x......x.x....xx..xx
63504x..x...........x...x....x........x.................................x....x.x....x..............
100557....................x.x....x.......x..x...................xx.x.....................x.......x..
92153..x........x.............x......x...........................xx.....x.x...x...x................
72824..x........x.......................x.................................x.....xx.x...............


Detailed Results

1. 23/94 matches (24%).
Acc. #: Species: HUMAN Name: Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
Index: MW: 52619 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
896.4590896.4453154734790(R) (F)
912.4496912.4402101Met-ox 4734790(R)SGMFWLR(F)
971.4989971.5274-291031110(K) (E)
1024.52031024.51049.71271340(R) (A)
1043.54711043.5137324734801(R) (-)
1058.60621058.5846204334420(R) (E)
1059.54201059.5086321Met-ox 4734801(R)SGMFWLRF(-)
1100.61251100.5740354244320(R) (I)
1256.71431256.6751314234321(R) (I)
1337.67951337.6371323793900(R) (G)
1440.75941440.7011402142250(R) (A)
1664.82801664.7702351121260(K) (E)
1680.82481680.7652361Met-ox 1121260(K)EVESMGAHLNAYSTR(E)
1764.96961764.8921442142281(R) (M)
2068.08302068.0133343974150(R) (S)
2092.16092092.1225182292480(R) (H)
2108.19612108.1174371Met-ox 2292480(R)MVLAAAGGVEHQQLLDLAQK(H)
2368.25242368.1760322492690(K) (F)
2377.23602377.14583859800(R) (F)
2633.36732633.2742351Met-ox 1031261(K)NRPGSALEKEVESMGAHLNAYSTR(E)
2659.43072659.3249401431651(K) (D)
2716.33602716.2717244484700(K) (I)
3130.57293130.55984.21812090(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 50% (244/480AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/94 matches (10%).
Acc. #: Species: MOUSE Name: Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial precursor
Index: MW: 52769 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
896.4590896.4453154734790(R) (F)
912.4496912.4402101Met-ox 4734790(R)SGMFWLR(F)
1043.54711043.5137324734801(R) (-)
1059.54201059.5086321Met-ox 4734801(R)SGMFWLRF(-)
1100.61251100.5740354244320(R) (I)
1256.71431256.6751314234321(R) (I)
2068.08302068.0133343974150(R) (S)
2092.16092092.1225182292480(R) (H)
2108.19612108.1174371Met-ox 2292480(R)MVLAAAGGVEHQQLLDLAQK(H)
2284.25762284.1760364244421(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (67/480AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/94 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.53071065.4999291541620(K) (L)
1081.54251081.4948441Met-ox 1541620(K)STMQELNSR(L)
1081.54251081.51392634451(R) (F)
1140.58921140.564921pyroGlu 2502600(R)QGVDADINGLR(Q)
1277.64061277.58754229401(R) (G)
1307.71771307.6782302402491(R) (Q)
1791.80151791.7283414905120(R) (G)
1838.01981837.9660293743890(R) (K)
1852.00951851.9275443213351(K) (N)
2377.23602377.1887201631831(R) (I)
2705.24062705.16172963940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (154/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/94 matches (10%).
Acc. #: Species: MOUSE Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
Index: MW: 99647 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4529909.4253302682740(R) (Y)
1011.52931011.5587-296216290(K) (H)
1111.53221111.5788-4212210(K) (Q)
1254.68211254.6920-7.92142230(K) (V)
1849.93271849.91579.28358540(K) (S)
1849.93271849.9563-137727860(K) (E)
1852.00951851.9427361Met-ox 5635790(R)HGFNVGQTMFSLLVTGK(L)
1852.00951852.00094.73123261(R) (E)
2068.08302067.98434867831(R) (K)
2077.06692077.1268-295635811(R) (R)
2095.06022095.1513-442072231(R) (V)
2225.20452225.097848AcetN 1211(-)GEVTAEEVEKFLDSNIGFAK(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (140/859AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/94 matches (7%).
Acc. #: Species: MOUSE Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 70042 Da pI: 8.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4529909.4729-223533591(R) (K)
1011.52931011.5587-291521600(K) (M)
1277.64061277.6101241611700(R) (E)
2077.06692077.0388143343520(R) (Q)
2195.07502195.1204-214304490(K) (Y)
2211.19192211.1153351Met-ox 4304490(K)EEIPVSNVAELLHQVAMGMK(Y)
2233.18152233.1399193333521(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (66/618AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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