MS-Fit Search Results

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Sample ID (comment): HSG001-0706
Database searched: SwissProt.6.17.2000
Molecular weight search (1000 - 100000 Da) selects 82508 entries.
Full pI range: 87272 entries.
Species search ( HUMAN MOUSE ) selects 9626 entries.
Combined molecular weight, pI and species searches select 8686 entries.
MS-Fit search selects 437 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
32

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.65e+003	7/32 (21%)	49990.2 / 6.85	MOUSE	HOMOGENTISATE 1,2-DIOXYGENASE (HOMOGENTISICASE) (HOMOGENTISATE OXYGENASE) (HOMOGENTISIC ACID OXIDASE)
2	2.36e+003	14/32 (43%)	18012.6 / 7.68	HUMAN	PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (PPIASE) (ROTAMASE) (CYCLOPHILIN A) (CYCLOSPORIN A-BINDING PROTEIN)
3	1.55e+003	12/32 (37%)	17971.4 / 7.73	MOUSE	PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (PPIASE) (ROTAMASE) (CYCLOPHILIN A) (CYCLOSPORIN A-BINDING PROTEIN) (SP18)
4	408	6/32 (18%)	56278.6 / 8.43	HUMAN	CYTOCHROME P450 2B6 (CYPIIB6) (P450 IIB1)
5	256	4/32 (12%)	49973.1 / 6.54	HUMAN	HOMOGENTISATE 1,2-DIOXYGENASE (HOMOGENTISICASE) (HOMOGENTISATE OXYGENASE) (HOMOGENTISIC ACID OXIDASE)

Detailed Results

1. 7/32 matches (21%). 49990.2 Da, pI = 6.85. Acc. # O09173. MOUSE. HOMOGENTISATE 1,2-DIOXYGENASE (HOMOGENTISICASE) (HOMOGENTISATE OXYGENASE) (HOMOGENTISIC ACID OXIDASE).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1523.7728	1523.7535	12.6896	226	237	(R)(R)
1530.8367	1530.7772	38.8391	172	184	(K)(G)
1644.7725	1644.6964	46.2610	6	20	(K)(C)
1946.0242	1945.9774	24.0639	172	187	(K)MSLQPNEICVIQRGMR(F)	1Cys-am
2119.1411	2119.0455	45.0804	391	410	(R)(W)
2135.1160	2135.0405	35.3767	391	410	(R)IADGTMAFMFESSLSLAVTK(W)	1Met-ox
2151.1268	2151.0354	42.5251	391	410	(R)IADGTMAFMFESSLSLAVTK(W)	2Met-ox

25 unmatched masses: 706.3043 737.3646 848.4084 1055.5523 1154.5901 1261.6631 1310.5933 1379.7752 1463.1287 1477.0383 1521.7969 1537.7667 1568.0863 1573.7877 1577.4299 1612.8085 1628.7915 1831.9347 1853.9149 1988.0629 2074.3284 2075.2314 2211.1633 2762.3411 2822.4213

The matched peptides cover 14% (63/445 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/32 matches (43%). 18012.6 Da, pI = 7.68. Acc. # P05092. HUMAN. PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (PPIASE) (ROTAMASE) (CYCLOPHILIN A) (CYCLOSPORIN A-BINDING PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
706.3043 706.3095 -7.2975 50 55 (K)(I)
737.3646 737.3582 8.6503 32 37 (K)(A)
848.4084 848.4154 -8.2427 119 125 (K)(H)
1055.5523 1055.5413 10.4107 20 28 (R)(V)
1154.5901 1154.5734 14.4612 83 91 (K)(H)
1261.6631 1261.6462 13.4238 155 165 (K)KITIADCGQLE(-) 1Cys-am
1310.5933 1310.5721 16.2389 134 144 (K)EGMNIVEAMER(F) 2Met-ox
1379.7752 1379.7575 12.8566 20 31 (R)(T)
1521.7969 1521.7405 37.0325 132 144 (K)VKEGMNIVEAMER(F) 1Met-ox
1537.7667 1537.7354 20.3359 132 144 (K)VKEGMNIVEAMER(F) 2Met-ox
1612.8085 1612.7616 29.0899 56 69 (R)IIPGFMCQGGDFTR(H) 1Cys-am
1628.7915 1628.7565 21.4664 56 69 (R)IIPGFMCQGGDFTR(H) 1Met-ox 1Cys-am
1831.9347 1831.9118 12.5107 77 91 (K)(H)
1988.0629 1988.0129 25.1535 1 19 (-)VNPTVFFDIAVDGEPLGR(V) Acet N

18 unmatched masses: 1463.1287 1477.0383 1523.7728 1530.8367 1568.0863 1573.7877 1577.4299 1644.7725 1853.9149 1946.0242 2074.3284 2075.2314 2119.1411 2135.1160 2151.1268 2211.1633 2762.3411 2822.4213

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
706.3043	706.3095	-7.2975	50	55	(K)(I)
737.3646	737.3582	8.6503	32	37	(K)(A)
848.4084	848.4154	-8.2427	119	125	(K)(H)
1055.5523	1055.5413	10.4107	20	28	(R)(V)
1154.5901	1154.5734	14.4612	83	91	(K)(H)
1261.6631	1261.6462	13.4238	155	165	(K)KITIADCGQLE(-)	1Cys-am
1310.5933	1310.5721	16.2389	134	144	(K)EGMNIVEAMER(F)	2Met-ox
1379.7752	1379.7575	12.8566	20	31	(R)(T)
1521.7969	1521.7405	37.0325	132	144	(K)VKEGMNIVEAMER(F)	1Met-ox
1537.7667	1537.7354	20.3359	132	144	(K)VKEGMNIVEAMER(F)	2Met-ox
1612.8085	1612.7616	29.0899	56	69	(R)IIPGFMCQGGDFTR(H)	1Cys-am
1628.7915	1628.7565	21.4664	56	69	(R)IIPGFMCQGGDFTR(H)	1Met-ox 1Cys-am
1831.9347	1831.9118	12.5107	77	91	(K)(H)
1988.0629	1988.0129	25.1535	1	19	(-)VNPTVFFDIAVDGEPLGR(V)	Acet N

The matched peptides cover 61% (102/165 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/32 matches (37%). 17971.4 Da, pI = 7.73. Acc. # P17742. MOUSE. PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (PPIASE) (ROTAMASE) (CYCLOPHILIN A) (CYCLOSPORIN A-BINDING PROTEIN) (SP18).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
737.3646 737.3582 8.6503 32 37 (K)(A)
848.4084 848.4154 -8.2427 119 125 (K)(H)
1055.5523 1055.5413 10.4107 20 28 (R)(V)
1154.5901 1154.5734 14.4612 83 91 (K)(H)
1310.5933 1310.5721 16.2389 134 144 (K)EGMNIVEAMER(F) 2Met-ox
1379.7752 1379.7575 12.8566 20 31 (R)(T)
1521.7969 1521.7405 37.0325 132 144 (K)VKEGMNIVEAMER(F) 1Met-ox
1537.7667 1537.7354 20.3359 132 144 (K)VKEGMNIVEAMER(F) 2Met-ox
1612.8085 1612.7616 29.0899 56 69 (R)IIPGFMCQGGDFTR(H) 1Cys-am
1628.7915 1628.7565 21.4664 56 69 (R)IIPGFMCQGGDFTR(H) 1Met-ox 1Cys-am
1831.9347 1831.9118 12.5107 77 91 (R)(H)
2211.1633 2211.1556 3.5064 145 164 (R)FGSRNGKTSKKITISDCGQL(-) 1Cys-am

20 unmatched masses: 706.3043 1261.6631 1463.1287 1477.0383 1523.7728 1530.8367 1568.0863 1573.7877 1577.4299 1644.7725 1853.9149 1946.0242 1988.0629 2074.3284 2075.2314 2119.1411 2135.1160 2151.1268 2762.3411 2822.4213

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
737.3646	737.3582	8.6503	32	37	(K)(A)
848.4084	848.4154	-8.2427	119	125	(K)(H)
1055.5523	1055.5413	10.4107	20	28	(R)(V)
1154.5901	1154.5734	14.4612	83	91	(K)(H)
1310.5933	1310.5721	16.2389	134	144	(K)EGMNIVEAMER(F)	2Met-ox
1379.7752	1379.7575	12.8566	20	31	(R)(T)
1521.7969	1521.7405	37.0325	132	144	(K)VKEGMNIVEAMER(F)	1Met-ox
1537.7667	1537.7354	20.3359	132	144	(K)VKEGMNIVEAMER(F)	2Met-ox
1612.8085	1612.7616	29.0899	56	69	(R)IIPGFMCQGGDFTR(H)	1Cys-am
1628.7915	1628.7565	21.4664	56	69	(R)IIPGFMCQGGDFTR(H)	1Met-ox 1Cys-am
1831.9347	1831.9118	12.5107	77	91	(R)(H)
2211.1633	2211.1556	3.5064	145	164	(R)FGSRNGKTSKKITISDCGQL(-)	1Cys-am

The matched peptides cover 52% (86/164 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/32 matches (18%). 56278.6 Da, pI = 8.43. Acc. # P20813. HUMAN. CYTOCHROME P450 2B6 (CYPIIB6) (P450 IIB1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
737.3646 737.3582 8.6503 92 98 (K)(G)
1573.7877 1573.7896 -1.1938 146 158 (R)(K)
1612.8085 1612.8045 2.4829 346 358 (K)(F)
1628.7915 1628.7994 -4.8792 346 358 (K)MPYTEAVIYEIQR(F) 1Met-ox
1644.7725 1644.8267 -32.9522 146 158 (R)IQEEAQCLIEELR(K) 1Cys-am
1988.0629 1988.0487 7.1788 146 161 (R)IQEEAQCLIEELRKSK(G) 1Cys-am

26 unmatched masses: 706.3043 848.4084 1055.5523 1154.5901 1261.6631 1310.5933 1379.7752 1463.1287 1477.0383 1521.7969 1523.7728 1530.8367 1537.7667 1568.0863 1577.4299 1831.9347 1853.9149 1946.0242 2074.3284 2075.2314 2119.1411 2135.1160 2151.1268 2211.1633 2762.3411 2822.4213

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
737.3646	737.3582	8.6503	92	98	(K)(G)
1573.7877	1573.7896	-1.1938	146	158	(R)(K)
1612.8085	1612.8045	2.4829	346	358	(K)(F)
1628.7915	1628.7994	-4.8792	346	358	(K)MPYTEAVIYEIQR(F)	1Met-ox
1644.7725	1644.8267	-32.9522	146	158	(R)IQEEAQCLIEELR(K)	1Cys-am
1988.0629	1988.0487	7.1788	146	161	(R)IQEEAQCLIEELRKSK(G)	1Cys-am

The matched peptides cover 7% (36/491 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/32 matches (12%). 49973.1 Da, pI = 6.54. Acc. # Q93099. HUMAN. HOMOGENTISATE 1,2-DIOXYGENASE (HOMOGENTISICASE) (HOMOGENTISATE OXYGENASE) (HOMOGENTISIC ACID OXIDASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1537.7667 1537.7691 -1.5577 226 237 (R)(Q)
2119.1411 2119.0455 45.0804 391 410 (R)(W)
2135.1160 2135.0405 35.3767 391 410 (R)IADGTMAFMFESSLSLAVTK(W) 1Met-ox
2151.1268 2151.0354 42.5251 391 410 (R)IADGTMAFMFESSLSLAVTK(W) 2Met-ox

28 unmatched masses: 706.3043 737.3646 848.4084 1055.5523 1154.5901 1261.6631 1310.5933 1379.7752 1463.1287 1477.0383 1521.7969 1523.7728 1530.8367 1568.0863 1573.7877 1577.4299 1612.8085 1628.7915 1644.7725 1831.9347 1853.9149 1946.0242 1988.0629 2074.3284 2075.2314 2211.1633 2762.3411 2822.4213

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1537.7667	1537.7691	-1.5577	226	237	(R)(Q)
2119.1411	2119.0455	45.0804	391	410	(R)(W)
2135.1160	2135.0405	35.3767	391	410	(R)IADGTMAFMFESSLSLAVTK(W)	1Met-ox
2151.1268	2151.0354	42.5251	391	410	(R)IADGTMAFMFESSLSLAVTK(W)	2Met-ox

The matched peptides cover 7% (32/445 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):