MS-Fit Search Results

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Sample ID (comment): HSG03
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 135 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	253	7/20 (35%)	41737.0 / 5.29	HUMAN	ACTIN, CYTOPLASMIC 1 (BETA-ACTIN).
1	253	6/20 (30%)	41793.1 / 5.31	HUMAN	ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN).
2	179	4/20 (20%)	6070.4 / 8.38	HUMAN	METALLOTHIONEIN-IG (MT-1G).
3	83.1	4/20 (20%)	35876.2 / 6.60	HUMAN	GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, MUSCLE (EC 1.2.1.12).
4	54.8	4/20 (20%)	42019.2 / 5.23	HUMAN	ACTIN, ALPHA CARDIAC.

Detailed Results

1. 7/20 matches (35%). 41737.0 Da, pI = 5.29. Acc. # P02570. HUMAN. ACTIN, CYTOPLASMIC 1 (BETA-ACTIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1198.6191	1198.7061	-72.5703	29	39	(R)(H)
1198.6191	1198.5228	80.3276	51	61	(K)(R)
1515.6511	1515.7497	-65.0228	85	95	(K)(V)
1790.7640	1790.8925	-71.7324	239	254	(K)(F)
1940.7976	1940.8404	-22.0200	1	18	(-)MDDDIAALVVDNGSGMCK(A)	1Met-ox 1Cys-am
1953.9383	1954.0650	-64.8321	96	113	(R)(A)
2230.9568	2231.0654	-48.7074	292	312	(K)DLYANTVLSGGTTMYPGIADR(M)	1Met-ox
3199.4631	3199.6098	-45.8375	148	177	(R)TTGIVMDSGDGVTHTVPIYEGYALPHAILR(L)	1Met-ox

13 unmatched masses: 524.1031 541.1662 568.0767 655.9974 671.9874 825.0420 890.4741 2096.8518 2210.9901 2299.0570 2300.0466 2383.8462 2705.0236

The matched peptides cover 36% (136/375 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

1. 6/20 matches (30%). 41793.1 Da, pI = 5.31. Acc. # P02571. HUMAN. ACTIN, CYTOPLASMIC 2 (GAMMA-ACTIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1198.6191 1198.7061 -72.5703 29 39 (R)(H)
1198.6191 1198.5228 80.3276 51 61 (K)(R)
1515.6511 1515.7497 -65.0228 85 95 (K)(V)
1790.7640 1790.8925 -71.7324 239 254 (K)(F)
1953.9383 1954.0650 -64.8321 96 113 (R)(A)
2230.9568 2231.0654 -48.7074 292 312 (K)DLYANTVLSGGTTMYPGIADR(M) 1Met-ox
3199.4631 3199.6098 -45.8375 148 177 (R)TTGIVMDSGDGVTHTVPIYEGYALPHAILR(L) 1Met-ox

14 unmatched masses: 524.1031 541.1662 568.0767 655.9974 671.9874 825.0420 890.4741 1940.7976 2096.8518 2210.9901 2299.0570 2300.0466 2383.8462 2705.0236

The matched peptides cover 31% (118/375 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/20 matches (20%). 6070.4 Da, pI = 8.38. Acc. # P13640. HUMAN. METALLOTHIONEIN-IG (MT-1G)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
541.1662 541.2114 -83.4974 26 30 (K)(K)
1515.6511 1515.5887 41.2331 32 43 (K)SCCSCCPVGCAK(C) 5Cys-am
1953.9383 1953.6943 124.8643 1 20 (-)MDPNCSCAAGVSCTCASSCK(C) 1Met-ox
2383.8462 2383.9492 -43.1824 23 43 (K)ECKCTSCKKSCCSCCPVGCAK(C) 3Cys-am

16 unmatched masses: 524.1031 568.0767 655.9974 671.9874 825.0420 890.4741 1198.6191 1790.7640 1940.7976 2096.8518 2210.9901 2230.9568 2299.0570 2300.0466 2705.0236 3199.4631

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
541.1662	541.2114	-83.4974	26	30	(K)(K)
1515.6511	1515.5887	41.2331	32	43	(K)SCCSCCPVGCAK(C)	5Cys-am
1953.9383	1953.6943	124.8643	1	20	(-)MDPNCSCAAGVSCTCASSCK(C)	1Met-ox
2383.8462	2383.9492	-43.1824	23	43	(K)ECKCTSCKKSCCSCCPVGCAK(C)	3Cys-am

The matched peptides cover 67% (41/61 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 35876.2 Da, pI = 6.60. Acc. # P00354. HUMAN. GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, MUSCLE (EC 1.2.1.12)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1515.6511 1515.8032 -100.3101 186 199 (K)(G)
1790.7640 1790.9145 -84.0145 145 161 (K)IISNASCTTNCLAPLAK(V) 1Cys-am
2230.9568 2231.1357 -80.2004 118 138 (R)(Y)
2299.0570 2299.2662 -90.9796 66 85 (K)(W)

16 unmatched masses: 524.1031 541.1662 568.0767 655.9974 671.9874 825.0420 890.4741 1198.6191 1940.7976 1953.9383 2096.8518 2210.9901 2300.0466 2383.8462 2705.0236 3199.4631

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1515.6511	1515.8032	-100.3101	186	199	(K)(G)
1790.7640	1790.9145	-84.0145	145	161	(K)IISNASCTTNCLAPLAK(V)	1Cys-am
2230.9568	2231.1357	-80.2004	118	138	(R)(Y)
2299.0570	2299.2662	-90.9796	66	85	(K)(W)

The matched peptides cover 21% (72/334 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 42019.2 Da, pI = 5.23. Acc. # P04270. HUMAN. ACTIN, ALPHA CARDIAC..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1198.6191 1198.7061 -72.5703 31 41 (R)(H)
1198.6191 1198.5228 80.3276 53 63 (K)(R)
1515.6511 1515.7497 -65.0228 87 97 (K)(V)
1790.7640 1790.8925 -71.7324 241 256 (K)(F)
3199.4631 3199.3982 20.2880 1 30 (-)MCDDEETTALVCDNGSGLVKAGFAGDDAPR(A) 2Cys-am

16 unmatched masses: 524.1031 541.1662 568.0767 655.9974 671.9874 825.0420 890.4741 1940.7976 1953.9383 2096.8518 2210.9901 2230.9568 2299.0570 2300.0466 2383.8462 2705.0236

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1198.6191	1198.7061	-72.5703	31	41	(R)(H)
1198.6191	1198.5228	80.3276	53	63	(K)(R)
1515.6511	1515.7497	-65.0228	87	97	(K)(V)
1790.7640	1790.8925	-71.7324	241	256	(K)(F)
3199.4631	3199.3982	20.2880	1	30	(-)MCDDEETTALVCDNGSGLVKAGFAGDDAPR(A)	2Cys-am

The matched peptides cover 20% (79/377 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):