MS-Fit Search Results

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Sample ID (comment): Magic Bullet digest
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 315 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
3

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
17

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	88.6	4/17 (23%)	17143.3 / 5.76	MOUSE	STATHMIN (PHOSPHOPROTEIN P19) (PP19) (ONCOPROTEIN 18) (OP18) (LEUKEMIA-ASSOCIATED PHOSPHOPROTEIN P18) (PP17) (PROSOLIN) (METABLASTIN) (PR22 PROTEIN).
2	88.4	4/17 (23%)	17171.4 / 5.77	HUMAN	STATHMIN (PHOSPHOPROTEIN P19) (PP19) (ONCOPROTEIN 18) (OP18) (LEUKEMIA-ASSOCIATED PHOSPHOPROTEIN P18) (PP17) (PROSOLIN) (METABLASTIN) (PR22 PROTEIN).
3	63.6	4/17 (23%)	53268.5 / 5.74	MOUSE	LAMIN B3.
4	39.9	3/17 (17%)	7009.3 / 7.76	MOUSE	METALLOTHIONEIN-III (MT-III) (GROWTH INHIBITORY FACTOR) (GIF).
5	35.8	3/17 (17%)	13131.3 / 8.60	MOUSE	LEYDIG INSULIN-LIKE PEPTIDE PRECURSOR (LEY-I-L) (RELAXIN-LIKE FACTOR) (RLF).

Detailed Results

1. 4/17 matches (23%). 17143.3 Da, pI = 5.76. Acc. # P54227. MOUSE. STATHMIN (PHOSPHOPROTEIN P19) (PP19) (ONCOPROTEIN 18) (OP18) (LEUKEMIA-ASSOCIATED PHOSPHOPROTEIN P18) (PP17) (PROSOLIN) (METABLASTIN) (PR22 PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
588.3110	588.3357	-41.9629	70	74	(K)(R)
1388.9969	1388.7538	175.0839	14	26	(R)(S)
2233.4855	2233.0923	176.0877	85	103	(K)(M)
2249.4759	2249.0872	172.8085	85	103	(K)AIEENNNFSKMAEEKLTHK(M)	1Met-ox

13 unmatched masses: 524.2274 543.2577 550.2198 568.2264 659.4008 672.1578 688.1326 834.4315 842.6618 885.5886 1941.2881 2211.5052 2299.5494

The matched peptides cover 25% (37/148 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/17 matches (23%). 17171.4 Da, pI = 5.77. Acc. # P16949. HUMAN. STATHMIN (PHOSPHOPROTEIN P19) (PP19) (ONCOPROTEIN 18) (OP18) (LEUKEMIA-ASSOCIATED PHOSPHOPROTEIN P18) (PP17) (PROSOLIN) (METABLASTIN) (PR22 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
588.3110 588.3357 -41.9629 70 74 (K)(R)
1388.9969 1388.7538 175.0839 14 26 (R)(S)
2233.4855 2233.0923 176.0877 85 103 (K)(M)
2249.4759 2249.0872 172.8085 85 103 (K)AIEENNNFSKMAEEKLTHK(M) 1Met-ox

13 unmatched masses: 524.2274 543.2577 550.2198 568.2264 659.4008 672.1578 688.1326 834.4315 842.6618 885.5886 1941.2881 2211.5052 2299.5494

The matched peptides cover 25% (37/148 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/17 matches (23%). 53268.5 Da, pI = 5.74. Acc. # P48680. MOUSE. LAMIN B3..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
550.2198 550.2473 -49.9634 344 348 (K)(D)
1388.9969 1388.7273 194.1619 416 429 (R)(A)
2211.5052 2211.1257 171.6024 318 338 (R)(F)
2299.5494 2299.1312 181.9093 268 291 (R)(G)

13 unmatched masses: 524.2274 543.2577 568.2264 588.3110 659.4008 672.1578 688.1326 834.4315 842.6618 885.5886 1941.2881 2233.4855 2249.4759

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
550.2198	550.2473	-49.9634	344	348	(K)(D)
1388.9969	1388.7273	194.1619	416	429	(R)(A)
2211.5052	2211.1257	171.6024	318	338	(R)(F)
2299.5494	2299.1312	181.9093	268	291	(R)(G)

The matched peptides cover 13% (64/474 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 3/17 matches (17%). 7009.3 Da, pI = 7.76. Acc. # P28184. MOUSE. METALLOTHIONEIN-III (MT-III) (GROWTH INHIBITORY FACTOR) (GIF)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
568.2264 568.2223 7.1050 27 31 (K)(K)
2233.4855 2233.8366 -157.1738 1 21 (-)MDPETCPCPTGGSCTCSDKCK(C) 1Cys-am
2249.4759 2249.8315 -158.0826 1 21 (-)MDPETCPCPTGGSCTCSDKCK(C) 1Met-ox 1Cys-am

14 unmatched masses: 524.2274 543.2577 550.2198 588.3110 659.4008 672.1578 688.1326 834.4315 842.6618 885.5886 1388.9969 1941.2881 2211.5052 2299.5494

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
568.2264	568.2223	7.1050	27	31	(K)(K)
2233.4855	2233.8366	-157.1738	1	21	(-)MDPETCPCPTGGSCTCSDKCK(C)	1Cys-am
2249.4759	2249.8315	-158.0826	1	21	(-)MDPETCPCPTGGSCTCSDKCK(C)	1Met-ox 1Cys-am

The matched peptides cover 38% (26/68 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 3/17 matches (17%). 13131.3 Da, pI = 8.60. Acc. # O09107. MOUSE. LEYDIG INSULIN-LIKE PEPTIDE PRECURSOR (LEY-I-L) (RELAXIN-LIKE FACTOR) (RLF)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
588.3110 588.2928 30.9751 40 45 (R)(W)
659.4008 659.3299 107.5980 40 45 (R)VCGGPR(W) 1Cys-am
885.5886 885.4430 164.4578 18 25 (R)(A)

14 unmatched masses: 524.2274 543.2577 550.2198 568.2264 672.1578 688.1326 834.4315 842.6618 1388.9969 1941.2881 2211.5052 2233.4855 2249.4759 2299.5494

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
588.3110	588.2928	30.9751	40	45	(R)(W)
659.4008	659.3299	107.5980	40	45	(R)VCGGPR(W)	1Cys-am
885.5886	885.4430	164.4578	18	25	(R)(A)

The matched peptides cover 11% (14/121 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):