MS-Fit Search Results

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Sample ID (comment): HSG004
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 199 entries (results displayed for top 10 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.17e+003	11/20 (55%)	18502.6 / 8.22	HUMAN	COFILIN, NON-MUSCLE ISOFORM.
2	295	8/20 (40%)	18559.7 / 8.22	MOUSE	COFILIN, NON-MUSCLE ISOFORM.
3	105	4/20 (20%)	30419.8 / 10.39	MOUSE	ANDROGEN-INDUCED GROWTH FACTOR PRECURSOR (AIGF) (HBGF-8) (FIBROBLAST GROWTH FACTOR-8) (FGF-8).
4	76	4/20 (20%)	32562.5 / 5.90	HUMAN	DNA EXCISION REPAIR PROTEIN ERCC-1.
5	65.4	4/20 (20%)	49779.1 / 9.43	HUMAN	ZINC FINGER PROTEIN 83 (ZINC FINGER PROTEIN HPF1).
6	49.8	5/20 (25%)	34802.6 / 4.74	HUMAN	MELANOMA-ASSOCIATED ANTIGEN 12 (MAGE-12 ANTIGEN) (MAGE12F).
7	45	4/20 (20%)	71831.4 / 7.51	HUMAN	TYROSINE-PROTEIN KINASE ITK/TSK (EC 2.7.1.112) (T-CELL-SPECIFIC KINASE) (TYROSINE-PROTEIN KINASE LYK) (KINASE EMT).
8	41.8	4/20 (20%)	54390.0 / 7.53	HUMAN	ANNEXIN XI (CALCYCLIN-ASSOCIATED ANNEXIN 50) (CAP-50) (56 KD AUTOANTIGEN).
9	41.3	5/20 (25%)	86308.3 / 9.16	MOUSE	EXOSTOSIN-1 (PUTATIVE TUMOUR SUPPRESSOR PROTEIN EXT1) (MULTIPLE EXOSTOSES PROTEIN 1).
10	36.4	4/20 (20%)	47748.1 / 8.82	HUMAN	TRANSFORMING GROWTH FACTOR BETA 2 PRECURSOR (TGF-BETA 2) (GLIOBLASTOMA -DERIVED T-CELL SUPPRESSOR FACTOR) (G-TSF) (BSC-1 CELL GROWTH INHIBITOR) (POLYERGIN) (CETERMIN).

Detailed Results

1. 11/20 matches (55%). 18502.6 Da, pI = 8.22. Acc. # P23528. HUMAN. COFILIN, NON-MUSCLE ISOFORM..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1024.6523	1024.5250	124.3108	14	21	(K)VFNDMKVR(K)	1Met-ox
1048.6327	1048.4920	134.2587	74	81	(K)MLPDKDCR(Y)	1Cys-am
1064.6142	1064.4869	119.6121	74	81	(K)MLPDKDCR(Y)	1Met-ox 1Cys-am
1337.7919	1337.6265	123.6284	82	92	(R)(E)
1340.9035	1340.7789	92.8694	153	166	(K)(-)
1805.0311	1804.8288	112.0833	133	146	(K)HELQANCYEEVKDR(C)	1Cys-am
1990.2888	1990.0690	110.4410	96	112	(K)(S)
2317.4233	2317.1611	113.1655	128	146	(K)LTGIKHELQANCYEEVKDR(C)	1Cys-am
2334.5146	2334.2386	118.2730	93	112	(K)(S)
2445.5326	2445.2560	113.1283	127	146	(K)KLTGIKHELQANCYEEVKDR(C)	1Cys-am
3062.9819	3062.5938	126.7459	46	73	(K)(M)

9 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1359.7650 1941.1501 2211.3729 2356.5040

The matched peptides cover 65% (108/166 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/20 matches (40%). 18559.7 Da, pI = 8.22. Acc. # P18760. MOUSE. COFILIN, NON-MUSCLE ISOFORM..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1024.6523 1024.5250 124.3108 14 21 (K)VFNDMKVR(K) 1Met-ox
1048.6327 1048.4920 134.2587 74 81 (K)MLPDKDCR(Y) 1Cys-am
1064.6142 1064.4869 119.6121 74 81 (K)MLPDKDCR(Y) 1Met-ox 1Cys-am
1337.7919 1337.6265 123.6284 82 92 (R)(E)
1340.9035 1340.7789 92.8694 153 166 (K)(-)
1805.0311 1804.8288 112.0833 133 146 (K)HELQANCYEEVKDR(C) 1Cys-am
2317.4233 2317.1611 113.1655 128 146 (K)LTGIKHELQANCYEEVKDR(C) 1Cys-am
2445.5326 2445.2560 113.1283 127 146 (K)KLTGIKHELQANCYEEVKDR(C) 1Cys-am

12 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1359.7650 1941.1501 1990.2888 2211.3729 2334.5146 2356.5040 3062.9819

The matched peptides cover 36% (60/166 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 30419.8 Da, pI = 10.39. Acc. # P37237. MOUSE. ANDROGEN-INDUCED GROWTH FACTOR PRECURSOR (AIGF) (HBGF-8) (FIBROBLAST GROWTH FACTOR-8) (FGF-8)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
568.2133 568.2956 -144.7992 220 223 (R)(E)
1064.6142 1064.6329 -17.5511 117 125 (K)(I)
2334.5146 2334.0975 178.6962 32 54 (R)(S)
2334.5146 2334.1115 172.7319 57 77 (R)(D)
2356.5040 2356.1495 150.4762 179 198 (K)DCVFTEIVLENNYTALQNAK(Y) 1Cys-am

16 unmatched masses: 585.4330 599.4568 656.1473 672.1265 1024.6523 1048.6327 1337.7919 1340.9035 1359.7650 1805.0311 1941.1501 1990.2888 2211.3729 2317.4233 2445.5326 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
568.2133	568.2956	-144.7992	220	223	(R)(E)
1064.6142	1064.6329	-17.5511	117	125	(K)(I)
2334.5146	2334.0975	178.6962	32	54	(R)(S)
2334.5146	2334.1115	172.7319	57	77	(R)(D)
2356.5040	2356.1495	150.4762	179	198	(K)DCVFTEIVLENNYTALQNAK(Y)	1Cys-am

The matched peptides cover 28% (77/268 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 32562.5 Da, pI = 5.90. Acc. # P07992. HUMAN. DNA EXCISION REPAIR PROTEIN ERCC-1..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1064.6142 1064.5662 45.1163 235 243 (R)VTECLTTVK(S) 1Cys-am
1340.9035 1340.6884 160.4003 269 281 (R)(A)
2334.5146 2334.1296 164.9512 188 207 (K)MCILADCTLILAWSPEEAGR(Y) 2Cys-am
3062.9819 3062.6614 104.6763 269 295 (R)(V)

16 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1024.6523 1048.6327 1337.7919 1359.7650 1805.0311 1941.1501 1990.2888 2211.3729 2317.4233 2356.5040 2445.5326

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1064.6142	1064.5662	45.1163	235	243	(R)VTECLTTVK(S)	1Cys-am
1340.9035	1340.6884	160.4003	269	281	(R)(A)
2334.5146	2334.1296	164.9512	188	207	(K)MCILADCTLILAWSPEEAGR(Y)	2Cys-am
3062.9819	3062.6614	104.6763	269	295	(R)(V)

The matched peptides cover 18% (56/297 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/20 matches (20%). 49779.1 Da, pI = 9.43. Acc. # P51522. HUMAN. ZINC FINGER PROTEIN 83 (ZINC FINGER PROTEIN HPF1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1337.7919 1337.7078 62.8311 349 360 (K)(K)
1990.2888 1990.1139 87.8601 13 29 (K)(E)
2334.5146 2334.1236 167.5463 340 360 (K)(K)
2356.5040 2356.1668 143.1416 175 193 (R)CNVCGKVFHHISHLAQHQR(I) 2Cys-am

16 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1024.6523 1048.6327 1064.6142 1340.9035 1359.7650 1805.0311 1941.1501 2211.3729 2317.4233 2445.5326 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1337.7919	1337.7078	62.8311	349	360	(K)(K)
1990.2888	1990.1139	87.8601	13	29	(K)(E)
2334.5146	2334.1236	167.5463	340	360	(K)(K)
2356.5040	2356.1668	143.1416	175	193	(R)CNVCGKVFHHISHLAQHQR(I)	2Cys-am

The matched peptides cover 13% (57/428 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/20 matches (25%). 34802.6 Da, pI = 4.74. Acc. # P43365. HUMAN. MELANOMA-ASSOCIATED ANTIGEN 12 (MAGE-12 ANTIGEN) (MAGE12F)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1048.6327 1048.4257 197.4532 212 220 (K)EGDCAPEEK(I) 1Cys-am
1337.7919 1337.9136 -90.9571 199 211 (K)(E)
1805.0311 1804.9744 31.4404 126 141 (R)(N)
2445.5326 2445.2641 109.8377 133 153 (K)(A)
3062.9819 3063.5654 -190.4467 128 153 (R)EPFTKAEMLGSVIRNFQDFFPVIFSK(A) 1Met-ox
3062.9819 3063.5540 -186.7209 235 259 (R)(Q)

15 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1024.6523 1064.6142 1340.9035 1359.7650 1941.1501 1990.2888 2211.3729 2317.4233 2334.5146 2356.5040

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1048.6327	1048.4257	197.4532	212	220	(K)EGDCAPEEK(I)	1Cys-am
1337.7919	1337.9136	-90.9571	199	211	(K)(E)
1805.0311	1804.9744	31.4404	126	141	(R)(N)
2445.5326	2445.2641	109.8377	133	153	(K)(A)
3062.9819	3063.5654	-190.4467	128	153	(R)EPFTKAEMLGSVIRNFQDFFPVIFSK(A)	1Met-ox
3062.9819	3063.5540	-186.7209	235	259	(R)(Q)

The matched peptides cover 23% (75/314 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/20 matches (20%). 71831.4 Da, pI = 7.51. Acc. # Q08881. HUMAN. TYROSINE-PROTEIN KINASE ITK/TSK (EC 2.7.1.112) (T-CELL-SPECIFIC KINASE) (TYROSINE-PROTEIN KINASE LYK) (KINASE EMT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1359.7650 1359.6769 64.7586 597 607 (K)(L)
2211.3729 2211.1861 84.4741 336 355 (R)(W)
2356.5040 2356.1766 138.9553 78 96 (K)(E)
2445.5326 2445.0988 177.4456 392 412 (K)(L)

16 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1024.6523 1048.6327 1064.6142 1337.7919 1340.9035 1805.0311 1941.1501 1990.2888 2317.4233 2334.5146 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1359.7650	1359.6769	64.7586	597	607	(K)(L)
2211.3729	2211.1861	84.4741	336	355	(R)(W)
2356.5040	2356.1766	138.9553	78	96	(K)(E)
2445.5326	2445.0988	177.4456	392	412	(K)(L)

The matched peptides cover 11% (71/620 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/20 matches (20%). 54390.0 Da, pI = 7.53. Acc. # P50995. HUMAN. ANNEXIN XI (CALCYCLIN-ASSOCIATED ANNEXIN 50) (CAP-50) (56 KD AUTOANTIGEN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1337.7919 1337.7357 42.0203 272 282 (K)(E)
1359.7650 1359.6909 54.5212 192 204 (R)(D)
1359.7650 1359.7895 -18.0003 451 461 (K)(S)
2211.3729 2211.0902 127.8453 211 230 (R)KAMKGFGTDEQAIIDCLGSR(S) 1Cys-am
2356.5040 2356.2400 112.0492 244 264 (K)(T)

16 unmatched masses: 568.2133 585.4330 599.4568 656.1473 672.1265 1024.6523 1048.6327 1064.6142 1340.9035 1805.0311 1941.1501 1990.2888 2317.4233 2334.5146 2445.5326 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1337.7919	1337.7357	42.0203	272	282	(K)(E)
1359.7650	1359.6909	54.5212	192	204	(R)(D)
1359.7650	1359.7895	-18.0003	451	461	(K)(S)
2211.3729	2211.0902	127.8453	211	230	(R)KAMKGFGTDEQAIIDCLGSR(S)	1Cys-am
2356.5040	2356.2400	112.0492	244	264	(K)(T)

The matched peptides cover 15% (76/505 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/20 matches (25%). 86308.3 Da, pI = 9.16. Acc. # P97464. MOUSE. EXOSTOSIN-1 (PUTATIVE TUMOUR SUPPRESSOR PROTEIN EXT1) (MULTIPLE EXOSTOSES PROTEIN 1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
585.4330 585.4088 41.3529 401 405 (K)(Q)
1024.6523 1024.5468 103.0487 116 123 (K)(G)
1024.6523 1024.4924 156.0824 240 248 (K)(G)
1337.7919 1337.6423 111.8461 33 43 (R)(N)
1941.1501 1940.8085 176.0233 312 325 (R)CDRDNTEYEKYDYR(E) 1Cys-am
2211.3729 2211.1232 112.9122 281 299 (R)NALYHVHNGEDVLLLTTCK(H) 1Cys-am

15 unmatched masses: 568.2133 599.4568 656.1473 672.1265 1048.6327 1064.6142 1340.9035 1359.7650 1805.0311 1990.2888 2317.4233 2334.5146 2356.5040 2445.5326 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
585.4330	585.4088	41.3529	401	405	(K)(Q)
1024.6523	1024.5468	103.0487	116	123	(K)(G)
1024.6523	1024.4924	156.0824	240	248	(K)(G)
1337.7919	1337.6423	111.8461	33	43	(R)(N)
1941.1501	1940.8085	176.0233	312	325	(R)CDRDNTEYEKYDYR(E)	1Cys-am
2211.3729	2211.1232	112.9122	281	299	(R)NALYHVHNGEDVLLLTTCK(H)	1Cys-am

The matched peptides cover 8% (66/746 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/20 matches (20%). 47748.1 Da, pI = 8.82. Acc. # P08112. HUMAN. TRANSFORMING GROWTH FACTOR BETA 2 PRECURSOR (TGF-BETA 2) (GLIOBLASTOMA -DERIVED T-CELL SUPPRESSOR FACTOR) (G-TSF) (BSC-1 CELL GROWTH INHIBITOR) (POLYERGIN) (CETERMIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
599.4568 599.3517 175.3066 154 158 (R)(A)
1941.1501 1940.9363 110.1758 312 327 (R)(R)
1990.2888 1989.9769 156.7028 250 268 (R)(S)
2334.5146 2334.1577 152.9005 250 271 (R)(K)

16 unmatched masses: 568.2133 585.4330 656.1473 672.1265 1024.6523 1048.6327 1064.6142 1337.7919 1340.9035 1359.7650 1805.0311 2211.3729 2317.4233 2356.5040 2445.5326 3062.9819

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
599.4568	599.3517	175.3066	154	158	(R)(A)
1941.1501	1940.9363	110.1758	312	327	(R)(R)
1990.2888	1989.9769	156.7028	250	268	(R)(S)
2334.5146	2334.1577	152.9005	250	271	(R)(K)

The matched peptides cover 10% (43/414 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):