MS-Fit Search Results

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Sample ID (comment): HSG007
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 279 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
22

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.42e+003	6/22 (27%)	14923.1 / 8.47	HUMAN	PROFILIN I.
2	314	7/22 (31%)	81900.1 / 4.97	HUMAN	TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303).
3	122	5/22 (22%)	48182.1 / 6.15	MOUSE	DNA-BINDING PROTEIN IKAROS.
3	122	8/22 (36%)	85632.5 / 7.06	HUMAN	INTEGRIN BETA-8 SUBUNIT PRECURSOR.
4	101	4/22 (18%)	24393.6 / 6.12	HUMAN	RAS-RELATED PROTEIN RAB-11A (RAB-11) (24KG) (YL8).

Detailed Results

1. 6/22 matches (27%). 14923.1 Da, pI = 8.47. Acc. # P07737. HUMAN. PROFILIN I..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
577.3318	577.3132	32.2742	70	74	(K)(D)
1379.7520	1379.7171	25.3163	91	104	(K)(T)
1470.8078	1470.7593	33.0272	56	69	(R)(C)
1625.8007	1625.7481	32.3261	75	88	(R)(T)
1641.7854	1641.7430	25.8362	75	88	(R)DSLLQDGEFSMDLR(T)	1Met-ox
1915.1271	1915.0653	32.2707	38	55	(K)(S)

16 unmatched masses: 541.2313 568.1482 585.3597 588.2440 599.3919 656.0694 1471.7888 1581.0357 1593.7730 1623.7539 1629.7298 1644.8728 1657.7640 2124.2916 2232.0846 2384.0634

The matched peptides cover 46% (65/139 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/22 matches (31%). 81900.1 Da, pI = 4.97. Acc. # O14867. HUMAN. TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
568.1482 568.1925 -77.8529 732 736 (K)(-)
577.3318 577.2946 64.5411 387 391 (R)(E)
1593.7730 1593.7947 -13.5652 31 44 (K)(F)
1623.7539 1623.8165 -38.5267 550 562 (K)LTPEQLDCIHDIR(R) 1Cys-am
1625.8007 1625.7039 59.5447 279 292 (K)CDESKLAMEPEETK(K) 1Met-ox
1644.8728 1644.6998 105.1940 717 731 (R)(C)
2232.0846 2232.0528 14.2237 284 303 (K)(S)

15 unmatched masses: 541.2313 585.3597 588.2440 599.3919 656.0694 1379.7520 1470.8078 1471.7888 1581.0357 1629.7298 1641.7854 1657.7640 1915.1271 2124.2916 2384.0634

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
568.1482	568.1925	-77.8529	732	736	(K)(-)
577.3318	577.2946	64.5411	387	391	(R)(E)
1593.7730	1593.7947	-13.5652	31	44	(K)(F)
1623.7539	1623.8165	-38.5267	550	562	(K)LTPEQLDCIHDIR(R)	1Cys-am
1625.8007	1625.7039	59.5447	279	292	(K)CDESKLAMEPEETK(K)	1Met-ox
1644.8728	1644.6998	105.1940	717	731	(R)(C)
2232.0846	2232.0528	14.2237	284	303	(K)(S)

The matched peptides cover 10% (76/736 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/22 matches (22%). 48182.1 Da, pI = 6.15. Acc. # Q03267. MOUSE. DNA-BINDING PROTEIN IKAROS..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1625.8007 1625.6787 75.0174 1 15 (-)(E)
1629.7298 1629.7742 -27.2285 107 121 (R)(S)
1641.7854 1641.6736 68.1114 1 15 (-)MDVDEGQDMSQVSGK(E) 1Met-ox
1644.8728 1644.9219 -29.8740 179 193 (R)(F)
1657.7640 1657.6685 57.5863 1 15 (-)MDVDEGQDMSQVSGK(E) 2Met-ox

17 unmatched masses: 541.2313 568.1482 577.3318 585.3597 588.2440 599.3919 656.0694 1379.7520 1470.8078 1471.7888 1581.0357 1593.7730 1623.7539 1915.1271 2124.2916 2232.0846 2384.0634

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1625.8007	1625.6787	75.0174	1	15	(-)(E)
1629.7298	1629.7742	-27.2285	107	121	(R)(S)
1641.7854	1641.6736	68.1114	1	15	(-)MDVDEGQDMSQVSGK(E)	1Met-ox
1644.8728	1644.9219	-29.8740	179	193	(R)(F)
1657.7640	1657.6685	57.5863	1	15	(-)MDVDEGQDMSQVSGK(E)	2Met-ox

The matched peptides cover 10% (45/431 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/22 matches (36%). 85632.5 Da, pI = 7.06. Acc. # P26012. HUMAN. INTEGRIN BETA-8 SUBUNIT PRECURSOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
577.3318 577.2227 189.1157 531 535 (K)(I)
1470.8078 1470.8433 -24.0877 706 717 (R)(S)
1623.7539 1623.8052 -31.6090 476 490 (R)(C)
1641.7854 1641.6606 76.0614 605 618 (R)GTCVCGRCECTDPR(S) 2Cys-am
1644.8728 1644.7903 50.1480 317 331 (K)(L)
1657.7640 1657.9277 -98.7566 332 346 (K)(Q)
2124.2916 2123.8724 197.3694 481 498 (K)(C)
2232.0846 2232.0694 6.7895 1 21 (-)CGSALAFFTAAFVCLQNDRR(G) Acet N

14 unmatched masses: 541.2313 568.1482 585.3597 588.2440 599.3919 656.0694 1379.7520 1471.7888 1581.0357 1593.7730 1625.8007 1629.7298 1915.1271 2384.0634

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
577.3318	577.2227	189.1157	531	535	(K)(I)
1470.8078	1470.8433	-24.0877	706	717	(R)(S)
1623.7539	1623.8052	-31.6090	476	490	(R)(C)
1641.7854	1641.6606	76.0614	605	618	(R)GTCVCGRCECTDPR(S)	2Cys-am
1644.8728	1644.7903	50.1480	317	331	(K)(L)
1657.7640	1657.9277	-98.7566	332	346	(K)(Q)
2124.2916	2123.8724	197.3694	481	498	(K)(C)
2232.0846	2232.0694	6.7895	1	21	(-)CGSALAFFTAAFVCLQNDRR(G)	Acet N

The matched peptides cover 13% (105/769 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/22 matches (18%). 24393.6 Da, pI = 6.12. Acc. # P24410. HUMAN. RAS-RELATED PROTEIN RAB-11A (RAB-11) (24KG) (YL8)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1593.7730 1593.7774 -2.7172 62 74 (K)(A)
1625.8007 1625.8321 -19.3441 111 125 (R)(S)
1641.7854 1641.8270 -25.3303 111 125 (R)DHADSNIVIMLVGNK(S) 1Met-ox
2232.0846 2232.1076 -10.3256 5 24 (R)(S)

18 unmatched masses: 541.2313 568.1482 577.3318 585.3597 588.2440 599.3919 656.0694 1379.7520 1470.8078 1471.7888 1581.0357 1623.7539 1629.7298 1644.8728 1657.7640 1915.1271 2124.2916 2384.0634

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1593.7730	1593.7774	-2.7172	62	74	(K)(A)
1625.8007	1625.8321	-19.3441	111	125	(R)(S)
1641.7854	1641.8270	-25.3303	111	125	(R)DHADSNIVIMLVGNK(S)	1Met-ox
2232.0846	2232.1076	-10.3256	5	24	(R)(S)

The matched peptides cover 22% (48/216 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):