MS-Fit Search Results

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Sample ID (comment): HSG8
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 112 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.55e+003	11/20 (55%)	17298.1 / 8.52	HUMAN	NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF).
2	2.62e+003	10/20 (50%)	17363.2 / 6.97	MOUSE	NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-M2) (P18).
3	101	4/20 (20%)	66035.3 / 6.33	HUMAN	INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN PRECURSOR (ALS).
4	77.6	5/20 (25%)	57116.1 / 8.08	HUMAN	ALPHA-1-FETOPROTEIN TRANSCRIPTION FACTOR.
5	77.2	5/20 (25%)	65104.9 / 8.45	HUMAN	ACYLOXYACYL HYDROLASE PRECURSOR (EC 3.-.-.-).

Detailed Results

1. 11/20 matches (55%). 17298.1 Da, pI = 8.52. Acc. # P22392. HUMAN. NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1175.6995	1175.6577	35.5475	57	66	(K)(Y)
1180.4818	1180.4733	7.1887	144	152	(K)SCAHDWVYE(-)	1Cys-am
1344.8249	1344.7640	45.2954	7	18	(R)(G)
1516.9132	1516.8851	18.5234	19	31	(R)(G)
1801.9828	1801.9118	39.3758	89	105	(R)VMLGETNPADSKPGTIR(G)	1Met-ox
2073.2610	2073.1286	63.8873	50	66	(K)(Y)
2075.1783	2075.1072	34.2724	1	18	(-)MANLERTFIAIKPDGVQR(G)	1Met-ox
2125.1795	2125.0462	62.7048	67	85	(K)YMNSGPVVAMVWEGLNVVK(T)	2Met-ox
2311.3424	2311.1862	67.5728	125	143	(K)(S)
2867.7032	2867.5208	63.6045	43	66	(R)(Y)
2868.6317	2868.6133	6.3989	1	26	(-)(R)

9 unmatched masses: 541.2123 550.1192 568.1655 656.0999 1730.8551 2126.1703 2211.1872 2233.1663 2312.2630

The matched peptides cover 77% (118/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/20 matches (50%). 17363.2 Da, pI = 6.97. Acc. # Q01768. MOUSE. NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-M2) (P18)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1175.6995 1175.6577 35.5475 57 66 (K)(Y)
1180.4818 1180.4733 7.1887 144 152 (K)SCAHDWVYE(-) 1Cys-am
1344.8249 1344.7640 45.2954 7 18 (R)(G)
1516.9132 1516.8851 18.5234 19 31 (R)(G)
1801.9828 1801.9118 39.3758 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2073.2610 2073.1286 63.8873 50 66 (K)(Y)
2075.1783 2075.1072 34.2724 1 18 (-)MANLERTFIAIKPDGVQR(G) 1Met-ox
2125.1795 2125.0462 62.7048 67 85 (K)YMNSGPVVAMVWEGLNVVK(T) 2Met-ox
2867.7032 2867.5208 63.6045 43 66 (R)(Y)
2868.6317 2868.6133 6.3989 1 26 (-)(R)

10 unmatched masses: 541.2123 550.1192 568.1655 656.0999 1730.8551 2126.1703 2211.1872 2233.1663 2311.3424 2312.2630

The matched peptides cover 65% (99/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 66035.3 Da, pI = 6.33. Acc. # P35858. HUMAN. INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN PRECURSOR (ALS)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1730.8551 1730.8035 29.8169 531 545 (R)(A)
1801.9828 1801.8406 78.9207 531 545 (R)LWLEGNPWDCGCPLK(A) 1Cys-am
2073.2610 2073.1973 30.7247 235 251 (K)(N)
2211.1872 2211.1886 -0.6447 136 157 (R)(L)

16 unmatched masses: 541.2123 550.1192 568.1655 656.0999 1175.6995 1180.4818 1344.8249 1516.9132 2075.1783 2125.1795 2126.1703 2233.1663 2311.3424 2312.2630 2867.7032 2868.6317

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1730.8551	1730.8035	29.8169	531	545	(R)(A)
1801.9828	1801.8406	78.9207	531	545	(R)LWLEGNPWDCGCPLK(A)	1Cys-am
2073.2610	2073.1973	30.7247	235	251	(K)(N)
2211.1872	2211.1886	-0.6447	136	157	(R)(L)

The matched peptides cover 8% (54/605 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/20 matches (25%). 57116.1 Da, pI = 8.08. Acc. # O00482. HUMAN. ALPHA-1-FETOPROTEIN TRANSCRIPTION FACTOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1730.8551 1730.7882 38.6318 53 67 (K)VSGYHYGLLTCESCK(G) 1Cys-am
1801.9828 1801.8253 87.3884 53 67 (K)VSGYHYGLLTCESCK(G) 2Cys-am
2233.1663 2233.1215 20.0912 462 479 (R)LPEIRAISMQAEEYLYYK(H) 1Met-ox
2312.2630 2312.1240 60.1282 79 96 (K)RYTCIENQNCQIDKTQRK(R) 1Cys-am
2312.2630 2312.1240 60.1282 80 97 (R)YTCIENQNCQIDKTQRKR(C) 1Cys-am
2868.6317 2868.3845 86.1868 73 95 (R)TVQNNKRYTCIENQNCQIDKTQR(K) 1Cys-am

15 unmatched masses: 541.2123 550.1192 568.1655 656.0999 1175.6995 1180.4818 1344.8249 1516.9132 2073.2610 2075.1783 2125.1795 2126.1703 2211.1872 2311.3424 2867.7032

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1730.8551	1730.7882	38.6318	53	67	(K)VSGYHYGLLTCESCK(G)	1Cys-am
1801.9828	1801.8253	87.3884	53	67	(K)VSGYHYGLLTCESCK(G)	2Cys-am
2233.1663	2233.1215	20.0912	462	479	(R)LPEIRAISMQAEEYLYYK(H)	1Met-ox
2312.2630	2312.1240	60.1282	79	96	(K)RYTCIENQNCQIDKTQRK(R)	1Cys-am
2312.2630	2312.1240	60.1282	80	97	(R)YTCIENQNCQIDKTQRKR(C)	1Cys-am
2868.6317	2868.3845	86.1868	73	95	(R)TVQNNKRYTCIENQNCQIDKTQR(K)	1Cys-am

The matched peptides cover 11% (58/500 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/20 matches (25%). 65104.9 Da, pI = 8.45. Acc. # P28039. HUMAN. ACYLOXYACYL HYDROLASE PRECURSOR (EC 3.-.-.-)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1730.8551 1730.9652 -63.6483 477 492 (R)(F)
2125.1795 2124.9768 95.3661 97 115 (K)LLSADMNADVVCHTLEFCK(Q) 1Met-ox
2311.3424 2311.1379 88.4650 323 339 (R)LWKRNHCNHRDYQNISR(N) 1Cys-am
2312.2630 2312.1671 41.4893 359 379 (K)VLDYPAIVIYAMIGNDVCSGK(S) 1Cys-am
2868.6317 2868.3496 98.3547 493 514 (K)(R)

15 unmatched masses: 541.2123 550.1192 568.1655 656.0999 1175.6995 1180.4818 1344.8249 1516.9132 1801.9828 2073.2610 2075.1783 2126.1703 2211.1872 2233.1663 2867.7032

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1730.8551	1730.9652	-63.6483	477	492	(R)(F)
2125.1795	2124.9768	95.3661	97	115	(K)LLSADMNADVVCHTLEFCK(Q)	1Met-ox
2311.3424	2311.1379	88.4650	323	339	(R)LWKRNHCNHRDYQNISR(N)	1Cys-am
2312.2630	2312.1671	41.4893	359	379	(K)VLDYPAIVIYAMIGNDVCSGK(S)	1Cys-am
2868.6317	2868.3496	98.3547	493	514	(K)(R)

The matched peptides cover 16% (95/575 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):