MS-Fit Search Results

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Sample ID (comment): HSG09
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 210 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.09e+003	12/20 (60%)	22110.5 / 8.27	HUMAN	THIOREDOXIN PEROXIDASE 2 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 2) (PROLIFERATION-ASSOCIATED PROTEIN PAG) (NATURAL KILLER CELL ENHANCING FACTOR A) (NKEF-A).
2	135	6/20 (30%)	22176.6 / 8.26	MOUSE	THIOREDOXIN PEROXIDASE 2 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 2) (OSTEOBLAST SPECIFIC FACTOR 3) (OSF-3) (MACROPHAGE 23 KD STRESS PROTEIN).
3	89.9	4/20 (20%)	59550.4 / 6.05	MOUSE	ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN).
4	58.8	4/20 (20%)	43262.5 / 5.69	HUMAN	CASPASE-4 PRECURSOR (EC 3.4.22.-) (CASP-4) (ICH-2 PROTEASE) (TX PROTEASE) (ICEREL-II).
5	56.9	4/20 (20%)	45230.8 / 5.78	MOUSE	TRANSCRIPTION FACTOR DP-1 (E2F DIMERIZATION PARTNER 1) (DRTF1- POLYPEPTIDE-1).

Detailed Results

1. 12/20 matches (60%). 22110.5 Da, pI = 8.27. Acc. # Q06830. HUMAN. THIOREDOXIN PEROXIDASE 2 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 2) (PROLIFERATION-ASSOCIATED PROTEIN PAG) (NATURAL KILLER CELL ENHANCING FACTOR A) (NKEF-A)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
819.3962	819.4212	-30.5934	152	158	(R)(L)
894.3942	894.4321	-42.4067	121	128	(K)(G)
1107.5847	1107.6050	-18.3432	111	120	(R)(A)
1180.5518	1180.5672	-13.0940	17	27	(K)ATAVMPDGQFK(D)	1Met-ox
1196.6125	1196.6315	-15.9206	159	168	(R)(H)
1211.6597	1211.6748	-12.4917	141	151	(R)(S)
1359.7987	1359.8000	-0.9431	129	140	(R)(Q)
1766.9069	1766.9475	-22.9466	93	109	(K)KQGGLGPMNIPLVSDPK(R)	1Met-ox
1794.9495	1794.9536	-2.3195	94	110	(K)QGGLGPMNIPLVSDPKR(T)	1Met-ox
1923.0774	1923.0486	14.9811	93	110	(K)KQGGLGPMNIPLVSDPKR(T)	1Met-ox
1983.0454	1983.0187	13.4491	111	128	(R)(G)
2102.0468	2102.0116	16.7335	17	35	(K)ATAVMPDGQFKDISLSDYK(G)	1Met-ox

8 unmatched masses: 568.0858 1194.6431 1734.1638 1777.9216 1862.1911 1940.9898 2104.1282 2211.1344

The matched peptides cover 47% (95/199 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/20 matches (30%). 22176.6 Da, pI = 8.26. Acc. # P35700. MOUSE. THIOREDOXIN PEROXIDASE 2 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 2) (OSTEOBLAST SPECIFIC FACTOR 3) (OSF-3) (MACROPHAGE 23 KD STRESS PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
894.3942 894.4321 -42.4067 121 128 (K)(G)
1107.5847 1107.6050 -18.3432 111 120 (R)(A)
1180.5518 1180.5672 -13.0940 17 27 (K)ATAVMPDGQFK(D) 1Met-ox
1196.6125 1196.6315 -15.9206 159 168 (R)(H)
1359.7987 1359.8000 -0.9431 129 140 (R)(Q)
1983.0454 1983.0187 13.4491 111 128 (R)(G)

14 unmatched masses: 568.0858 819.3962 1194.6431 1211.6597 1734.1638 1766.9069 1777.9216 1794.9495 1862.1911 1923.0774 1940.9898 2102.0468 2104.1282 2211.1344

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
894.3942	894.4321	-42.4067	121	128	(K)(G)
1107.5847	1107.6050	-18.3432	111	120	(R)(A)
1180.5518	1180.5672	-13.0940	17	27	(K)ATAVMPDGQFK(D)	1Met-ox
1196.6125	1196.6315	-15.9206	159	168	(R)(H)
1359.7987	1359.8000	-0.9431	129	140	(R)(Q)
1983.0454	1983.0187	13.4491	111	128	(R)(G)

The matched peptides cover 25% (51/199 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 59550.4 Da, pI = 6.05. Acc. # Q62158. MOUSE. ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1359.7987 1359.7120 63.7963 255 266 (R)(A)
1766.9069 1766.7413 93.7691 52 66 (R)CWGAAETNVSCPQCR(E) 2Cys-am
1983.0454 1982.9985 23.6666 363 379 (R)FNLFPCVLGSPCFIAGR(H) 2Cys-am
2102.0468 2102.0123 16.4074 89 107 (K)QLRTERPSGPGGEMGVCEK(H) 1Cys-am

16 unmatched masses: 568.0858 819.3962 894.3942 1107.5847 1180.5518 1194.6431 1196.6125 1211.6597 1734.1638 1777.9216 1794.9495 1862.1911 1923.0774 1940.9898 2104.1282 2211.1344

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1359.7987	1359.7120	63.7963	255	266	(R)(A)
1766.9069	1766.7413	93.7691	52	66	(R)CWGAAETNVSCPQCR(E)	2Cys-am
1983.0454	1982.9985	23.6666	363	379	(R)FNLFPCVLGSPCFIAGR(H)	2Cys-am
2102.0468	2102.0123	16.4074	89	107	(K)QLRTERPSGPGGEMGVCEK(H)	1Cys-am

The matched peptides cover 12% (63/522 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 43262.5 Da, pI = 5.69. Acc. # P49662. HUMAN. CASPASE-4 PRECURSOR (EC 3.4.22.-) (CASP-4) (ICH-2 PROTEASE) (TX PROTEASE) (ICEREL-II)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1211.6597 1211.7727 -93.3006 9 19 (K)(D)
1766.9069 1766.8417 36.9248 50 64 (K)(Q)
1923.0774 1923.0162 31.8106 137 152 (R)LALIICNTEFDHLPPR(N) 1Cys-am
2104.1282 2104.0459 39.1094 315 332 (R)DSTMGSIFITQLITCFQK(Y) 1Cys-am

16 unmatched masses: 568.0858 819.3962 894.3942 1107.5847 1180.5518 1194.6431 1196.6125 1359.7987 1734.1638 1777.9216 1794.9495 1862.1911 1940.9898 1983.0454 2102.0468 2211.1344

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1211.6597	1211.7727	-93.3006	9	19	(K)(D)
1766.9069	1766.8417	36.9248	50	64	(K)(Q)
1923.0774	1923.0162	31.8106	137	152	(R)LALIICNTEFDHLPPR(N)	1Cys-am
2104.1282	2104.0459	39.1094	315	332	(R)DSTMGSIFITQLITCFQK(Y)	1Cys-am

The matched peptides cover 15% (60/377 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/20 matches (20%). 45230.8 Da, pI = 5.78. Acc. # Q08639. MOUSE. TRANSCRIPTION FACTOR DP-1 (E2F DIMERIZATION PARTNER 1) (DRTF1- POLYPEPTIDE-1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1194.6431 1194.5676 63.2109 270 280 (K)(F)
1777.9216 1778.0176 -53.9938 27 44 (K)(Q)
1794.9495 1794.9498 -0.1803 169 184 (R)(E)
1983.0454 1983.0407 2.3560 168 184 (R)RVYDALNVLMAMNIISK(E) 2Met-ox

16 unmatched masses: 568.0858 819.3962 894.3942 1107.5847 1180.5518 1196.6125 1211.6597 1359.7987 1734.1638 1766.9069 1862.1911 1923.0774 1940.9898 2102.0468 2104.1282 2211.1344

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1194.6431	1194.5676	63.2109	270	280	(K)(F)
1777.9216	1778.0176	-53.9938	27	44	(K)(Q)
1794.9495	1794.9498	-0.1803	169	184	(R)(E)
1983.0454	1983.0407	2.3560	168	184	(R)RVYDALNVLMAMNIISK(E)	2Met-ox

The matched peptides cover 11% (46/410 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):