MS-Fit Search Results

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Sample ID (comment): HSG010
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9843 entries.
Combined molecular weight, pI and species searches select 8871 entries.
MS-Fit search selects 871 entries (results displayed for top 5 matches).
Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |
Min. # Peptides
to Match
4		 Peptide Mass
Tolerance (+/-)
100.000 ppm		 Peptide Masses
are
monoisotopic		 Digest
Used
Trypsin		 Max. # Missed
Cleavages
4		 Cysteines
Modified by
unmodified		 Peptide
N terminus
Hydrogen (H)		 Peptide
C terminus
Free Acid (O H)		 Input #
Peptide Masses
30

Result Summary

Rank MOWSE
Score		 # (%)
Masses
Matched		 Protein
MW (Da)/pI		 Species SwissProt.8.28.2000
Accession #

Protein Name
1 2.25e+003 6/30 (20%) 10192.5 / 6.54 HUMAN CYTOCHROME C OXIDASE POLYPEPTIDE VIB (AED)
2 1.86e+003 7/30 (23%) 11740.5 / 6.56 HUMAN CALGIZZARIN (S100C PROTEIN) (MLN 70)
3 540 5/30 (16%) 11082.8 / 5.28 MOUSE CALGIZZARIN (ENDOTHELIAL MONOCYTE-ACTIVATING POLYPEPTIDE) (EMAP)
4 530 4/30 (13%) 11287.0 / 7.76 HUMAN PUTATIVE S100-TYPE CALCIUM-BINDING PROTEIN RG276O03.3
5 239 7/30 (23%) 84502.1 / 6.48 MOUSE SEMAPHORIN 4F PRECURSOR (SEMAPHORIN W) (SEMA W)

Detailed Results


1. 6/30 matches (20%). 10192.5 Da, pI = 6.54. Acc. # P14854. HUMAN. CYTOCHROME C OXIDASE POLYPEPTIDE VIB (AED).
m/z
submitted		 MH+
matched		 Delta
ppm		 start end

Peptide Sequence
(Click for Fragment Ions)

Modifications

793.3874

793.3844

3.7416

 14 20

(K)(F)

1004.5238

1004.4914

32.3247

 21 28

(R)(N)

1483.7239

1483.6640

40.3843

 48 59

(K)GGDISVCEWYQR(V)

 1Cys-am

1566.7773

1566.6912

54.9316

 29 39

(R)NCWQNYLDFHR(C)

 1Cys-am

1778.9360

1778.8574

44.1919

 14 28

(K)(N)

2284.2084

2284.0345

76.1684

 60 77

(R)VYQSLCPTSWVTDWDEQR(A)

 1Cys-am

24 unmatched masses: 647.2403 652.2958 656.0855 771.4217 825.1457 842.5170 1019.5206 1141.6443 1275.7452 1321.7500 1475.8449 1479.7771 1638.9179 1850.0000 1865.9923 1888.0072 1941.0418 1945.0286 2195.0562 2211.2306 2233.2307 2299.2980 2384.1152 2705.3550

The matched peptides cover 65% (56/86 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


2. 7/30 matches (23%). 11740.5 Da, pI = 6.56. Acc. # P31949. HUMAN. CALGIZZARIN (S100C PROTEIN) (MLN 70).
m/z
submitted		 MH+
matched		 Delta
ppm		 start end

Peptide Sequence
(Click for Fragment Ions)

Modifications

771.4217

771.4001

27.9576

 56 62

(K)(M)

1019.5206

1019.5009

19.3233

 4 12

(K)(C)

1141.6443

1141.5966

41.8567

 53 62

(K)(M)

1321.7500

1321.7190

23.4891

 13 23

(R)CIESLIAVFQK(Y)

 1Cys-am

1321.7500

1321.6608

67.5006

 56 66

(K)DPGVLDRMMKK(L)

 2Met-ox

1479.7771

1479.7120

44.0354

 24 36

(K)(T)

1850.0000

1849.9046

51.5607

 37 52

(K)(N)

1865.9923

1865.8995

49.7244

 37 52

(K)TEFLSFMNTELAAFTK(N)

 1Met-ox

23 unmatched masses: 647.2403 652.2958 656.0855 793.3874 825.1457 842.5170 1004.5238 1275.7452 1475.8449 1483.7239 1566.7773 1638.9179 1778.9360 1888.0072 1941.0418 1945.0286 2195.0562 2211.2306 2233.2307 2284.2084 2299.2980 2384.1152 2705.3550

The matched peptides cover 60% (63/105 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


3. 5/30 matches (16%). 11082.8 Da, pI = 5.28. Acc. # P50543. MOUSE. CALGIZZARIN (ENDOTHELIAL MONOCYTE-ACTIVATING POLYPEPTIDE) (EMAP).
m/z
submitted		 MH+
matched		 Delta
ppm		 start end

Peptide Sequence
(Click for Fragment Ions)

Modifications

771.4217

771.4001

27.9576

 51 57

(K)(M)

1141.6443

1141.5966

41.8567

 48 57

(K)(M)

1321.7500

1321.7190

23.4891

 8 18

(R)CIESLIAVFQK(Y)

 1Cys-am

1321.7500

1321.6608

67.5006

 51 61

(K)DPGVLDRMMKK(L)

 2Met-ox

1850.0000

1849.9046

51.5607

 32 47

(K)(N)

1865.9923

1865.8995

49.7244

 32 47

(K)TEFLSFMNTELAAFTK(N)

 1Met-ox

25 unmatched masses: 647.2403 652.2958 656.0855 793.3874 825.1457 842.5170 1004.5238 1019.5206 1275.7452 1475.8449 1479.7771 1483.7239 1566.7773 1638.9179 1778.9360 1888.0072 1941.0418 1945.0286 2195.0562 2211.2306 2233.2307 2284.2084 2299.2980 2384.1152 2705.3550

The matched peptides cover 41% (41/98 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


4. 4/30 matches (13%). 11287.0 Da, pI = 7.76. Acc. # O60417. HUMAN. PUTATIVE S100-TYPE CALCIUM-BINDING PROTEIN RG276O03.3.
m/z
submitted		 MH+
matched		 Delta
ppm		 start end

Peptide Sequence
(Click for Fragment Ions)

Modifications

1019.5206

1019.5009

19.3233

 4 12

(K)(C)

1321.7500

1321.7190

23.4891

 13 23

(R)CIESLIAVFQK(Y)

 1Cys-am

1850.0000

1849.9046

51.5607

 37 52

(K)(N)

1865.9923

1865.8995

49.7244

 37 52

(K)TEFLSFMNTELAAFTK(N)

 1Met-ox

26 unmatched masses: 647.2403 652.2958 656.0855 771.4217 793.3874 825.1457 842.5170 1004.5238 1141.6443 1275.7452 1475.8449 1479.7771 1483.7239 1566.7773 1638.9179 1778.9360 1888.0072 1941.0418 1945.0286 2195.0562 2211.2306 2233.2307 2284.2084 2299.2980 2384.1152 2705.3550

The matched peptides cover 35% (36/102 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


5. 7/30 matches (23%). 84502.1 Da, pI = 6.48. Acc. # Q9Z123. MOUSE. SEMAPHORIN 4F PRECURSOR (SEMAPHORIN W) (SEMA W).
m/z
submitted		 MH+
matched		 Delta
ppm		 start end

Peptide Sequence
(Click for Fragment Ions)

Modifications

1566.7773

1566.7586

11.9036

 155 168

(K)(L)

1778.9360

1778.8305

59.3478

 554 570

(R)(E)

1850.0000

1849.8676

71.5834

 554 570

(R)GMVQDIESADVSSLCPK(E)

 1Cys-am

1865.9923

1865.8625

69.5754

 554 570

(R)GMVQDIESADVSSLCPK(E)

 1Met-ox 1Cys-am

1941.0418

1940.9336

55.7703

 105 119

(R)(K)

2211.2306

2211.1926

17.1942

 397 415

(K)(D)

2284.2084

2284.1555

23.1578

 105 121

(R)RIDWMVPETHRQNCRKK(G)

 1Met-ox 1Cys-am

23 unmatched masses: 647.2403 652.2958 656.0855 771.4217 793.3874 825.1457 842.5170 1004.5238 1019.5206 1141.6443 1275.7452 1321.7500 1475.8449 1479.7771 1483.7239 1638.9179 1888.0072 1945.0286 2195.0562 2233.2307 2299.2980 2384.1152 2705.3550

The matched peptides cover 8% (67/777 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit 3.1.1, ProteinProspector 3.2.1
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