MS-Fit Search Results

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Sample ID (comment): HSG09
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 206 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.89e+005	12/20 (60%)	26538.4 / 6.51	HUMAN	TRIOSEPHOSPHATE ISOMERASE (EC 5.3.1.1) (TIM).
2	422	7/20 (35%)	26581.6 / 7.09	MOUSE	TRIOSEPHOSPHATE ISOMERASE (EC 5.3.1.1) (TIM).
3	129	6/20 (30%)	99916.0 / 6.40	MOUSE	VAV2 PROTEIN.
4	89.4	4/20 (20%)	27368.4 / 8.78	HUMAN	RHO-RELATED GTP-BINDING PROTEIN RHO8.
5	85.5	5/20 (25%)	25988.9 / 5.58	MOUSE	RAS-RELATED PROTEIN RAB-20.

Detailed Results

1. 12/20 matches (60%). 26538.4 Da, pI = 6.51. Acc. # P00938. HUMAN. TRIOSEPHOSPHATE ISOMERASE (EC 5.3.1.1) (TIM)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
954.4950	954.4838	11.7691	6	13	(K)(M)
1082.6090	1082.5787	27.9410	5	13	(R)(M)
1234.6230	1234.6028	16.3889	194	205	(K)(I)
1458.7824	1458.7229	40.7861	100	112	(R)(V)
1466.7904	1466.7239	45.3234	175	187	(K)(L)
1542.9430	1542.8855	37.2503	18	32	(R)(V)
1600.8048	1600.7542	31.6155	85	98	(K)DCGATWVVLGHSER(R)	1Cys-am
1602.9062	1602.8896	10.3951	160	174	(K)(T)
1614.8886	1614.8240	40.0103	99	112	(R)(V)
1822.0123	1821.9897	12.4130	113	130	(K)VAHALAEGLGVIACIGEK(L)	1Cys-am
2206.1768	2206.0854	41.4133	33	52	(K)VPADTEVVCAPPTAYIDFAR(Q)	1Cys-am
3029.7545	3029.5835	56.4416	219	247	(K)(Q)

8 unmatched masses: 656.0890 825.1556 976.5108 1104.6162 1495.7943 1624.9610 2101.1463 2211.2273

The matched peptides cover 64% (159/248 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/20 matches (35%). 26581.6 Da, pI = 7.09. Acc. # P17751. MOUSE. TRIOSEPHOSPHATE ISOMERASE (EC 5.3.1.1) (TIM)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
954.4950 954.4838 11.7691 6 13 (K)(M)
1082.6090 1082.5787 27.9410 5 13 (R)(M)
1458.7824 1458.7229 40.7861 100 112 (R)(V)
1466.7904 1466.7239 45.3234 175 187 (K)(L)
1602.9062 1602.8896 10.3951 160 174 (K)(T)
1614.8886 1614.8240 40.0103 99 112 (R)(V)
3029.7545 3029.5835 56.4416 219 247 (K)(Q)

13 unmatched masses: 656.0890 825.1556 976.5108 1104.6162 1234.6230 1495.7943 1542.9430 1600.8048 1624.9610 1822.0123 2101.1463 2206.1768 2211.2273

The matched peptides cover 32% (80/248 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/20 matches (30%). 99916.0 Da, pI = 6.40. Acc. # Q60992. MOUSE. VAV2 PROTEIN..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1082.6090 1082.5747 31.6570 755 762 (K)(T)
1104.6162 1104.5802 32.5869 679 688 (K)(E)
1466.7904 1466.6871 70.4523 195 207 (R)(Y)
1602.9062 1602.9042 1.2839 318 330 (K)FKLQDLLVVPMQR(V) 1Met-ox
2101.1463 2101.0316 54.5850 727 743 (K)(E)
2206.1768 2206.1906 -6.2426 39 58 (R)DGVLLCQLLHNLSPGSIDLK(D) 1Cys-am

14 unmatched masses: 656.0890 825.1556 954.4950 976.5108 1234.6230 1458.7824 1495.7943 1542.9430 1600.8048 1614.8886 1624.9610 1822.0123 2211.2273 3029.7545

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1082.6090	1082.5747	31.6570	755	762	(K)(T)
1104.6162	1104.5802	32.5869	679	688	(K)(E)
1466.7904	1466.6871	70.4523	195	207	(R)(Y)
1602.9062	1602.9042	1.2839	318	330	(K)FKLQDLLVVPMQR(V)	1Met-ox
2101.1463	2101.0316	54.5850	727	743	(K)(E)
2206.1768	2206.1906	-6.2426	39	58	(R)DGVLLCQLLHNLSPGSIDLK(D)	1Cys-am

The matched peptides cover 9% (81/868 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 27368.4 Da, pI = 8.78. Acc. # P52199. HUMAN. RHO-RELATED GTP-BINDING PROTEIN RHO8..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1104.6162 1104.5723 39.7053 26 36 (K)(T)
1234.6230 1234.6652 -34.1568 130 140 (K)(T)
2206.1768 2206.1324 20.1232 6 25 (R)(I)
2211.2273 2211.0716 70.4267 168 187 (K)QIGAATYIECSALQSENSVR(D) 1Cys-am

16 unmatched masses: 656.0890 825.1556 954.4950 976.5108 1082.6090 1458.7824 1466.7904 1495.7943 1542.9430 1600.8048 1602.9062 1614.8886 1624.9610 1822.0123 2101.1463 3029.7545

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1104.6162	1104.5723	39.7053	26	36	(K)(T)
1234.6230	1234.6652	-34.1568	130	140	(K)(T)
2206.1768	2206.1324	20.1232	6	25	(R)(I)
2211.2273	2211.0716	70.4267	168	187	(K)QIGAATYIECSALQSENSVR(D)	1Cys-am

The matched peptides cover 25% (62/244 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/20 matches (25%). 25988.9 Da, pI = 5.58. Acc. # P35295. MOUSE. RAS-RELATED PROTEIN RAB-20..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
954.4950 954.4719 24.2215 163 169 (K)(E)
1104.6162 1104.6087 6.7642 136 146 (K)(Q)
1495.7943 1495.6586 90.6964 209 222 (R)(T)
1822.0123 1821.8540 86.8688 209 225 (R)(Q)
2211.2273 2211.1120 52.1503 95 114 (R)FLGLTETANNDCLFAIVGNK(V) 1Cys-am

15 unmatched masses: 656.0890 825.1556 976.5108 1082.6090 1234.6230 1458.7824 1466.7904 1542.9430 1600.8048 1602.9062 1614.8886 1624.9610 2101.1463 2206.1768 3029.7545

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
954.4950	954.4719	24.2215	163	169	(K)(E)
1104.6162	1104.6087	6.7642	136	146	(K)(Q)
1495.7943	1495.6586	90.6964	209	222	(R)(T)
1822.0123	1821.8540	86.8688	209	225	(R)(Q)
2211.2273	2211.1120	52.1503	95	114	(R)FLGLTETANNDCLFAIVGNK(V)	1Cys-am

The matched peptides cover 23% (55/233 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):