MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG12
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 264 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.92e+003	11/20 (55%)	28672.9 / 6.75	HUMAN	PHOSPHOGLYCERATE MUTASE, BRAIN FORM (EC 5.4.2.1) (PGAM-B) (EC 5.4.2.4) (EC 3.1.3.13) (BPG-DEPENDENT PGAM).
2	515	9/20 (45%)	67337.6 / 5.65	MOUSE	ALPHA-FETOPROTEIN PRECURSOR (ALPHA-FETOGLOBULIN) (ALPHA-1- FETOPROTEIN).
3	372	6/20 (30%)	27368.4 / 8.78	HUMAN	RHO-RELATED GTP-BINDING PROTEIN RHO8.
4	195	4/20 (20%)	28635.1 / 9.00	HUMAN	PHOSPHOGLYCERATE MUTASE, MUSCLE FORM (EC 5.4.2.1) (PGAM-M) (EC 5.4.2.4) (EC 3.1.3.13) (BPG-DEPENDENT PGAM).
5	136	4/20 (20%)	46084.8 / 8.62	MOUSE	COUP TRANSCRIPTION FACTOR 1 (COUP-TF1) (COUP-TFI).

Detailed Results

1. 11/20 matches (55%). 28672.9 Da, pI = 6.75. Acc. # P18669. HUMAN. PHOSPHOGLYCERATE MUTASE, BRAIN FORM (EC 5.4.2.1) (PGAM-B) (EC 5.4.2.4) (EC 3.1.3.13) (BPG-DEPENDENT PGAM)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1150.7624	1150.6697	80.5827	180	190	(R)(G)
1312.6717	1312.6034	51.9914	10	20	(R)(F)
1683.9855	1683.9110	44.2443	162	175	(R)(E)
1949.9910	1949.9067	43.2170	46	61	(R)DAGYEFDICFTSVQKR(A)	1Cys-am
1979.9615	1979.8775	42.3833	21	38	(R)(R)
2131.2295	2131.1222	50.3742	222	239	(K)NLKPIKPMQFLGDEETVR(K)	1Met-ox
2136.0701	2135.9787	42.8097	21	39	(R)(G)
2259.2964	2259.2171	35.1155	222	240	(K)NLKPIKPMQFLGDEETVRK(A)	1Met-ox
2433.2078	2433.1073	41.3001	117	137	(R)SYDVPPPPMEPDHPFYSNISK(D)	1Met-ox
2439.2802	2439.1713	44.6073	141	161	(R)YADLTEDQLPSCESLKDTIAR(A)	1Cys-am
2589.3551	2589.2084	56.6531	116	137	(R)RSYDVPPPPMEPDHPFYSNISK(D)	1Met-ox

9 unmatched masses: 1344.6427 1705.9689 2070.4792 2071.3499 2152.0725 2168.0699 2211.1822 2334.2585 2455.1895

The matched peptides cover 52% (133/253 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/20 matches (45%). 67337.6 Da, pI = 5.65. Acc. # P02772. MOUSE. ALPHA-FETOPROTEIN PRECURSOR (ALPHA-FETOGLOBULIN) (ALPHA-1- FETOPROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1312.6717 1312.5843 66.5405 191 201 (K)(R)
1979.9615 1980.0337 -36.4690 195 210 (K)EECFQTKRASIAKELR(E) 1Cys-am
2131.2295 2131.0534 82.6360 126 145 (K)(A)
2136.0701 2135.9829 40.8250 465 483 (K)WSGCGEGMADIFIGHLCIR(N) 1Cys-am
2152.0725 2151.9778 43.9915 465 483 (K)WSGCGEGMADIFIGHLCIR(N) 1Met-ox 1Cys-am
2168.0699 2168.0340 16.5390 514 531 (R)(D)
2259.2964 2259.1484 65.5476 125 145 (R)(A)
2334.2585 2334.0342 96.1114 380 399 (K)CSQSGNLPGCQDNLEEELQK(H) 2Cys-am
2433.2078 2433.1650 17.5723 280 300 (K)VMTYICSQQNILSSKIAECCK(L) 1Cys-am

11 unmatched masses: 1150.7624 1344.6427 1683.9855 1705.9689 1949.9910 2070.4792 2071.3499 2211.1822 2439.2802 2455.1895 2589.3551

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1312.6717	1312.5843	66.5405	191	201	(K)(R)
1979.9615	1980.0337	-36.4690	195	210	(K)EECFQTKRASIAKELR(E)	1Cys-am
2131.2295	2131.0534	82.6360	126	145	(K)(A)
2136.0701	2135.9829	40.8250	465	483	(K)WSGCGEGMADIFIGHLCIR(N)	1Cys-am
2152.0725	2151.9778	43.9915	465	483	(K)WSGCGEGMADIFIGHLCIR(N)	1Met-ox 1Cys-am
2168.0699	2168.0340	16.5390	514	531	(R)(D)
2259.2964	2259.1484	65.5476	125	145	(R)(A)
2334.2585	2334.0342	96.1114	380	399	(K)CSQSGNLPGCQDNLEEELQK(H)	2Cys-am
2433.2078	2433.1650	17.5723	280	300	(K)VMTYICSQQNILSSKIAECCK(L)	1Cys-am

The matched peptides cover 19% (119/605 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/20 matches (30%). 27368.4 Da, pI = 8.78. Acc. # P52199. HUMAN. RHO-RELATED GTP-BINDING PROTEIN RHO8..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1979.9615 1980.0337 -36.4690 217 234 (R)(K)
2131.2295 2131.1294 46.9905 5 23 (R)(C)
2136.0701 2136.1348 -30.2803 216 234 (K)(K)
2152.0725 2152.0605 5.5769 119 136 (K)GEIQEFCPNTKMLLVGCK(S) 2Cys-am
2152.0725 2152.1297 -26.5855 216 234 (K)RISHMPSRPELSAVATDLR(K) 1Met-ox
2168.0699 2168.0554 6.6932 119 136 (K)GEIQEFCPNTKMLLVGCK(S) 1Met-ox 2Cys-am
2211.1822 2211.0716 50.0515 168 187 (K)QIGAATYIECSALQSENSVR(D) 1Cys-am

14 unmatched masses: 1150.7624 1312.6717 1344.6427 1683.9855 1705.9689 1949.9910 2070.4792 2071.3499 2259.2964 2334.2585 2433.2078 2439.2802 2455.1895 2589.3551

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1979.9615	1980.0337	-36.4690	217	234	(R)(K)
2131.2295	2131.1294	46.9905	5	23	(R)(C)
2136.0701	2136.1348	-30.2803	216	234	(K)(K)
2152.0725	2152.0605	5.5769	119	136	(K)GEIQEFCPNTKMLLVGCK(S)	2Cys-am
2152.0725	2152.1297	-26.5855	216	234	(K)RISHMPSRPELSAVATDLR(K)	1Met-ox
2168.0699	2168.0554	6.6932	119	136	(K)GEIQEFCPNTKMLLVGCK(S)	1Met-ox 2Cys-am
2211.1822	2211.0716	50.0515	168	187	(K)QIGAATYIECSALQSENSVR(D)	1Cys-am

The matched peptides cover 31% (76/244 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 28635.1 Da, pI = 9.00. Acc. # P15259. HUMAN. PHOSPHOGLYCERATE MUTASE, MUSCLE FORM (EC 5.4.2.1) (PGAM-M) (EC 5.4.2.4) (EC 3.1.3.13) (BPG-DEPENDENT PGAM)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1150.7624 1150.6697 80.5827 180 190 (R)(G)
1683.9855 1683.9110 44.2443 162 175 (R)(A)
1705.9689 1705.8835 50.0449 141 156 (R)(D)
2259.2964 2259.1630 59.0827 46 64 (K)(T)

16 unmatched masses: 1312.6717 1344.6427 1949.9910 1979.9615 2070.4792 2071.3499 2131.2295 2136.0701 2152.0725 2168.0699 2211.1822 2334.2585 2433.2078 2439.2802 2455.1895 2589.3551

The matched peptides cover 23% (60/252 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/20 matches (20%). 46084.8 Da, pI = 8.62. Acc. # Q60632. MOUSE. COUP TRANSCRIPTION FACTOR 1 (COUP-TF1) (COUP-TFI)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
2211.1822 2210.9923 85.9179 70 91 (K)(S)
2439.2802 2439.1338 60.0030 92 111 (K)SSGKHYGQFTCEGCKSFFKR(S) 2Cys-am
2455.1895 2455.1658 9.6453 126 144 (R)NCPIDQHHRNQCQYCRLKK(C) 1Cys-am
2589.3551 2589.2870 26.2784 323 346 (K)AIVLFTSDACGLSDAAHIESLQEK(S) 1Cys-am

16 unmatched masses: 1150.7624 1312.6717 1344.6427 1683.9855 1705.9689 1949.9910 1979.9615 2070.4792 2071.3499 2131.2295 2136.0701 2152.0725 2168.0699 2259.2964 2334.2585 2433.2078

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
2211.1822	2210.9923	85.9179	70	91	(K)(S)
2439.2802	2439.1338	60.0030	92	111	(K)SSGKHYGQFTCEGCKSFFKR(S)	2Cys-am
2455.1895	2455.1658	9.6453	126	144	(R)NCPIDQHHRNQCQYCRLKK(C)	1Cys-am
2589.3551	2589.2870	26.2784	323	346	(K)AIVLFTSDACGLSDAAHIESLQEK(S)	1Cys-am

The matched peptides cover 20% (85/422 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):