MS-Fit Search Results

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Sample ID (comment): HSG13
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 85 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
150.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.03e+003	8/20 (40%)	36507.5 / 5.72	HUMAN	L-LACTATE DEHYDROGENASE H CHAIN (EC 1.1.1.27) (LDH-B).
2	276	6/20 (30%)	36441.3 / 5.70	MOUSE	L-LACTATE DEHYDROGENASE H CHAIN (EC 1.1.1.27) (LDH-B).
3	46.4	5/20 (25%)	86308.3 / 9.16	MOUSE	EXOSTOSIN-1 (PUTATIVE TUMOUR SUPPRESSOR PROTEIN EXT1) (MULTIPLE EXOSTOSES PROTEIN 1).
3	46.2	4/20 (20%)	33950.3 / 9.51	HUMAN	COLLAGEN ALPHA 2(VI) CHAIN (FRAGMENT).
4	34.9	5/20 (25%)	72258.3 / 5.94	MOUSE	HISTIDINE AMMONIA-LYASE (EC 4.3.1.3) (HISTIDASE).

Detailed Results

1. 8/20 matches (40%). 36507.5 Da, pI = 5.72. Acc. # P07195. HUMAN. L-LACTATE DEHYDROGENASE H CHAIN (EC 1.1.1.27) (LDH-B)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
742.4649	742.4575	9.9389	107	112	(R)(N)
913.6052	913.5835	23.7782	91	99	(K)(Q)
957.6117	957.6137	-2.1096	119	126	(K)(Y)
1176.6055	1176.5901	13.1488	319	328	(K)(D)
1283.6937	1283.6557	29.6224	233	243	(K)MVVESAYEVIK(L)	1Met-ox
1629.9168	1629.8587	35.6587	43	57	(K)(L)
1694.9341	1694.8965	22.1885	7	22	(K)(I)
2205.1740	2205.1048	31.3863	58	76	(K)LKGEMMDLQHGSLFLQTPK(I)	2Met-ox

12 unmatched masses: 523.1149 656.0744 672.0517 825.1148 841.1059 861.0908 877.0792 1716.9264 1940.9868 2211.1688 2233.1932 2299.2457

The matched peptides cover 28% (94/333 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/20 matches (30%). 36441.3 Da, pI = 5.70. Acc. # P16125. MOUSE. L-LACTATE DEHYDROGENASE H CHAIN (EC 1.1.1.27) (LDH-B)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
742.4649 742.4575 9.9389 107 112 (R)(N)
913.6052 913.5835 23.7782 91 99 (K)(Q)
957.6117 957.6137 -2.1096 119 126 (K)(Y)
1176.6055 1176.5901 13.1488 319 328 (K)(D)
1629.9168 1629.8587 35.6587 43 57 (K)(L)
2205.1740 2205.1048 31.3863 58 76 (K)LKGEMMDLQHGSLFLQTPK(I) 2Met-ox

14 unmatched masses: 523.1149 656.0744 672.0517 825.1148 841.1059 861.0908 877.0792 1283.6937 1694.9341 1716.9264 1940.9868 2211.1688 2233.1932 2299.2457

The matched peptides cover 20% (67/333 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/20 matches (25%). 86308.3 Da, pI = 9.16. Acc. # P97464. MOUSE. EXOSTOSIN-1 (PUTATIVE TUMOUR SUPPRESSOR PROTEIN EXT1) (MULTIPLE EXOSTOSES PROTEIN 1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
957.6117 957.5522 62.1559 253 260 (K)(K)
1940.9868 1940.8085 91.8600 312 325 (R)CDRDNTEYEKYDYR(E) 1Cys-am
2205.1740 2205.1854 -5.1803 262 280 (K)(N)
2211.1688 2211.1232 20.6116 281 299 (R)NALYHVHNGEDVLLLTTCK(H) 1Cys-am
2299.2457 2299.0496 85.2909 98 115 (K)CRMESCFDFTLCKKNGFK(V) 2Cys-am
2299.2457 2298.9686 120.5415 309 325 (K)DSRCDRDNTEYEKYDYR(E) 1Cys-am

15 unmatched masses: 523.1149 656.0744 672.0517 742.4649 825.1148 841.1059 861.0908 877.0792 913.6052 1176.6055 1283.6937 1629.9168 1694.9341 1716.9264 2233.1932

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
957.6117	957.5522	62.1559	253	260	(K)(K)
1940.9868	1940.8085	91.8600	312	325	(R)CDRDNTEYEKYDYR(E)	1Cys-am
2205.1740	2205.1854	-5.1803	262	280	(K)(N)
2211.1688	2211.1232	20.6116	281	299	(R)NALYHVHNGEDVLLLTTCK(H)	1Cys-am
2299.2457	2299.0496	85.2909	98	115	(K)CRMESCFDFTLCKKNGFK(V)	2Cys-am
2299.2457	2298.9686	120.5415	309	325	(K)DSRCDRDNTEYEKYDYR(E)	1Cys-am

The matched peptides cover 10% (81/746 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 33950.3 Da, pI = 9.51. Acc. # P12110. HUMAN. COLLAGEN ALPHA 2(VI) CHAIN (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
742.4649 742.3922 98.0030 102 108 (R)IGPPGCK(G) 1Cys-am
957.6117 957.5118 104.3598 309 317 (K)(G)
1283.6937 1283.6669 20.8712 12 24 (K)(G)
1629.9168 1629.7760 86.3835 336 353 (R)(-)

16 unmatched masses: 523.1149 656.0744 672.0517 825.1148 841.1059 861.0908 877.0792 913.6052 1176.6055 1694.9341 1716.9264 1940.9868 2205.1740 2211.1688 2233.1932 2299.2457

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
742.4649	742.3922	98.0030	102	108	(R)IGPPGCK(G)	1Cys-am
957.6117	957.5118	104.3598	309	317	(K)(G)
1283.6937	1283.6669	20.8712	12	24	(K)(G)
1629.9168	1629.7760	86.3835	336	353	(R)(-)

The matched peptides cover 13% (46/353 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/20 matches (25%). 72258.3 Da, pI = 5.94. Acc. # P35492. MOUSE. HISTIDINE AMMONIA-LYASE (EC 4.3.1.3) (HISTIDASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
742.4649 742.4463 25.0693 176 182 (R)(L)
1629.9168 1629.9660 -30.1974 207 220 (R)CRMLLALRINVLAK(G) 1Met-ox
1940.9868 1941.0446 -29.7721 452 469 (K)(R)
2211.1688 2211.0603 49.0488 416 435 (K)DIITTELNSATDNPMVFASR(G) 1Met-ox
2233.1932 2233.2192 -11.6675 249 272 (K)(M)
2233.1932 2233.0750 52.9085 368 386 (R)(F)

15 unmatched masses: 523.1149 656.0744 672.0517 825.1148 841.1059 861.0908 877.0792 913.6052 957.6117 1176.6055 1283.6937 1694.9341 1716.9264 2205.1740 2299.2457

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
742.4649	742.4463	25.0693	176	182	(R)(L)
1629.9168	1629.9660	-30.1974	207	220	(R)CRMLLALRINVLAK(G)	1Met-ox
1940.9868	1941.0446	-29.7721	452	469	(K)(R)
2211.1688	2211.0603	49.0488	416	435	(K)DIITTELNSATDNPMVFASR(G)	1Met-ox
2233.1932	2233.2192	-11.6675	249	272	(K)(M)
2233.1932	2233.0750	52.9085	368	386	(R)(F)

The matched peptides cover 15% (102/657 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):