MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG015
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 253 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
25

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.58e+004	9/25 (36%)	32560.2 / 4.62	MOUSE	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).
2	1.58e+004	9/25 (36%)	32575.2 / 4.64	HUMAN	NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38).
3	1.25e+003	6/25 (24%)	57747.0 / 7.57	HUMAN	PYRUVATE KINASE, M1 (MUSCLE) ISOZYME (EC 2.7.1.40) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1).
4	1.25e+003	6/25 (24%)	57783.0 / 7.95	HUMAN	PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40).
5	1.19e+003	4/25 (16%)	25565.2 / 4.67	HUMAN	CYTOCHROME C OXIDASE POLYPEPTIDE II (EC 1.9.3.1).

Detailed Results

1. 9/25 matches (36%). 32560.2 Da, pI = 4.62. Acc. # Q61937. MOUSE. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
539.2872	539.2400	87.4291	272	275	(K)(M)
931.5241	931.4737	54.1438	238	246	(K)(A)
1568.8026	1568.7305	45.9665	33	45	(K)(T)
1819.8925	1819.8437	26.7920	276	289	(R)(K)
1835.8879	1835.8387	26.8301	276	289	(R)MTDQEAIQDLWQWR(K)	1Met-ox
2161.0933	2161.0123	37.4703	55	73	(K)DELHIVEAEAMNYEGSPIK(V)	1Met-ox
2227.2576	2227.2161	18.6399	81	101	(K)(L)
2243.2838	2243.2110	32.4733	81	101	(K)MSVQPTVSLGGFEITPPVVLR(L)	1Met-ox
2945.5605	2945.4566	35.2559	46	73	(R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V)	1Met-ox

16 unmatched masses: 568.1811 656.1342 1296.7507 1775.1724 1776.1174 1777.0203 1791.8891 1835.3731 1851.9002 1867.9061 1941.0091 2093.1613 2182.4240 2211.1976 2242.7232 2465.2582

The matched peptides cover 30% (89/292 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/25 matches (36%). 32575.2 Da, pI = 4.64. Acc. # P06748. HUMAN. NUCLEOPHOSMIN (NPM) (NUCLEOLAR PHOSPHOPROTEIN B23) (NUMATRIN) (NUCLEOLAR PROTEIN NO38)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
539.2872 539.2400 87.4291 274 277 (K)(M)
931.5241 931.4737 54.1438 240 248 (K)(A)
1568.8026 1568.7305 45.9665 33 45 (K)(T)
1819.8925 1819.8437 26.7920 278 291 (R)(K)
1835.8879 1835.8387 26.8301 278 291 (R)MTDQEAIQDLWQWR(K) 1Met-ox
2161.0933 2161.0123 37.4703 55 73 (K)DELHIVEAEAMNYEGSPIK(V) 1Met-ox
2227.2576 2227.2161 18.6399 81 101 (K)(L)
2243.2838 2243.2110 32.4733 81 101 (K)MSVQPTVSLGGFEITPPVVLR(L) 1Met-ox
2945.5605 2945.4566 35.2559 46 73 (R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V) 1Met-ox

16 unmatched masses: 568.1811 656.1342 1296.7507 1775.1724 1776.1174 1777.0203 1791.8891 1835.3731 1851.9002 1867.9061 1941.0091 2093.1613 2182.4240 2211.1976 2242.7232 2465.2582

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
539.2872	539.2400	87.4291	274	277	(K)(M)
931.5241	931.4737	54.1438	240	248	(K)(A)
1568.8026	1568.7305	45.9665	33	45	(K)(T)
1819.8925	1819.8437	26.7920	278	291	(R)(K)
1835.8879	1835.8387	26.8301	278	291	(R)MTDQEAIQDLWQWR(K)	1Met-ox
2161.0933	2161.0123	37.4703	55	73	(K)DELHIVEAEAMNYEGSPIK(V)	1Met-ox
2227.2576	2227.2161	18.6399	81	101	(K)(L)
2243.2838	2243.2110	32.4733	81	101	(K)MSVQPTVSLGGFEITPPVVLR(L)	1Met-ox
2945.5605	2945.4566	35.2559	46	73	(R)TVSLGAGAKDELHIVEAEAMNYEGSPIK(V)	1Met-ox

The matched peptides cover 30% (89/294 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/25 matches (24%). 57747.0 Da, pI = 7.57. Acc. # P14618. HUMAN. PYRUVATE KINASE, M1 (MUSCLE) ISOZYME (EC 2.7.1.40) (CYTOSOLIC THYROID HORMONE-BINDING PROTEIN) (CTHBP) (THBP1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1819.8925 1819.9484 -30.7193 319 335 (R)(K)
1835.8879 1835.9433 -30.1802 319 335 (R)AGKPVICATQMLESMIK(K) 1Met-ox
1851.9002 1851.9382 -20.5232 319 335 (R)AGKPVICATQMLESMIK(K) 2Met-ox
1941.0091 1940.9250 43.3141 151 165 (K)CDENILWLDYKNICK(V) 1Cys-am
2161.0933 2161.2120 -54.9286 166 185 (K)(Q)
2465.2582 2465.2928 -14.0377 92 114 (R)(G)

19 unmatched masses: 539.2872 568.1811 656.1342 931.5241 1296.7507 1568.8026 1775.1724 1776.1174 1777.0203 1791.8891 1835.3731 1867.9061 2093.1613 2182.4240 2211.1976 2227.2576 2242.7232 2243.2838 2945.5605

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1819.8925	1819.9484	-30.7193	319	335	(R)(K)
1835.8879	1835.9433	-30.1802	319	335	(R)AGKPVICATQMLESMIK(K)	1Met-ox
1851.9002	1851.9382	-20.5232	319	335	(R)AGKPVICATQMLESMIK(K)	2Met-ox
1941.0091	1940.9250	43.3141	151	165	(K)CDENILWLDYKNICK(V)	1Cys-am
2161.0933	2161.2120	-54.9286	166	185	(K)(Q)
2465.2582	2465.2928	-14.0377	92	114	(R)(G)

The matched peptides cover 14% (75/530 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/25 matches (24%). 57783.0 Da, pI = 7.95. Acc. # P14786. HUMAN. PYRUVATE KINASE, M2 ISOZYME (EC 2.7.1.40)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1819.8925 1819.9484 -30.7193 319 335 (R)(K)
1835.8879 1835.9433 -30.1802 319 335 (R)AGKPVICATQMLESMIK(K) 1Met-ox
1851.9002 1851.9382 -20.5232 319 335 (R)AGKPVICATQMLESMIK(K) 2Met-ox
1941.0091 1940.9250 43.3141 151 165 (K)CDENILWLDYKNICK(V) 1Cys-am
2161.0933 2161.2120 -54.9286 166 185 (K)(Q)
2465.2582 2465.2928 -14.0377 92 114 (R)(G)

19 unmatched masses: 539.2872 568.1811 656.1342 931.5241 1296.7507 1568.8026 1775.1724 1776.1174 1777.0203 1791.8891 1835.3731 1867.9061 2093.1613 2182.4240 2211.1976 2227.2576 2242.7232 2243.2838 2945.5605

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1819.8925	1819.9484	-30.7193	319	335	(R)(K)
1835.8879	1835.9433	-30.1802	319	335	(R)AGKPVICATQMLESMIK(K)	1Met-ox
1851.9002	1851.9382	-20.5232	319	335	(R)AGKPVICATQMLESMIK(K)	2Met-ox
1941.0091	1940.9250	43.3141	151	165	(K)CDENILWLDYKNICK(V)	1Cys-am
2161.0933	2161.2120	-54.9286	166	185	(K)(Q)
2465.2582	2465.2928	-14.0377	92	114	(R)(G)

The matched peptides cover 14% (75/530 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/25 matches (16%). 25565.2 Da, pI = 4.67. Acc. # P00403. HUMAN. CYTOCHROME C OXIDASE POLYPEPTIDE II (EC 1.9.3.1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1851.9002 1851.9414 -22.2265 83 98 (R)(S)
1867.9061 1867.9363 -16.1733 83 98 (R)ILYMTDEVNDPSLTIK(S) 1Met-ox
2227.2576 2227.1619 42.9512 152 171 (R)(T)
2243.2838 2243.1568 56.6115 152 171 (R)MMITSQDVLHSWAVPTLGLK(T) 1Met-ox

21 unmatched masses: 539.2872 568.1811 656.1342 931.5241 1296.7507 1568.8026 1775.1724 1776.1174 1777.0203 1791.8891 1819.8925 1835.3731 1835.8879 1941.0091 2093.1613 2161.0933 2182.4240 2211.1976 2242.7232 2465.2582 2945.5605

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1851.9002	1851.9414	-22.2265	83	98	(R)(S)
1867.9061	1867.9363	-16.1733	83	98	(R)ILYMTDEVNDPSLTIK(S)	1Met-ox
2227.2576	2227.1619	42.9512	152	171	(R)(T)
2243.2838	2243.1568	56.6115	152	171	(R)MMITSQDVLHSWAVPTLGLK(T)	1Met-ox

The matched peptides cover 15% (36/227 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):