MS-Fit Search Results

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Sample ID (comment): HSG016
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 37 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
23

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.48e+004	9/23 (39%)	21892.0 / 5.66	HUMAN	THIOREDOXIN PEROXIDASE 1 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA) (PRP) (NATURAL KILLER CELL ENHANCING FACTOR B) (NKEF-B).
2	349	6/23 (26%)	21778.8 / 5.20	MOUSE	THIOREDOXIN PEROXIDASE 1 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA).
3	49.8	4/23 (17%)	72258.3 / 5.94	MOUSE	HISTIDINE AMMONIA-LYASE (EC 4.3.1.3) (HISTIDASE).
4	36.9	4/23 (17%)	67183.4 / 5.54	HUMAN	INTERFERON-INDUCED GUANYLATE-BINDING PROTEIN 2 (GUANINE NUCLEOTIDE- BINDING PROTEIN 2).
5	36.3	5/23 (21%)	78944.9 / 4.61	MOUSE	AMYLOID-LIKE PROTEIN 2 PRECURSOR (CDEI-BOX BINDING PROTEIN) (CDEBP).

Detailed Results

1. 9/23 matches (39%). 21892.0 Da, pI = 5.66. Acc. # P32119. HUMAN. THIOREDOXIN PEROXIDASE 1 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA) (PRP) (NATURAL KILLER CELL ENHANCING FACTOR B) (NKEF-B)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
789.3972	789.4107	-17.0966	151	157	(R)(L)
924.4299	924.4427	-13.8047	120	127	(K)(G)
924.4299	924.4216	9.0519	192	198	(K)(-)
1179.6582	1179.6374	17.6887	110	119	(R)(T)
1211.6793	1211.6748	3.6817	140	150	(R)(S)
1735.0061	1734.9754	17.6620	93	109	(K)(R)
1863.1072	1863.0704	19.7642	92	109	(R)(R)
1941.0228	1941.0333	-5.4402	17	34	(K)(G)
2713.4736	2713.3772	35.5328	68	91	(K)LGCEVLGVSVDSQFTHLAWINTPR(K)	1Cys-am
2841.5820	2841.4722	38.6458	67	91	(R)KLGCEVLGVSVDSQFTHLAWINTPR(K)	1Cys-am
2841.5820	2841.4722	38.6458	68	92	(K)LGCEVLGVSVDSQFTHLAWINTPRK(E)	1Cys-am

14 unmatched masses: 541.1996 568.1134 656.0546 825.1044 1194.6319 1296.7079 1465.7823 1475.7621 1567.7498 1716.9434 1868.0507 1885.1214 2384.0225 2465.2934

The matched peptides cover 52% (103/198 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/23 matches (26%). 21778.8 Da, pI = 5.20. Acc. # Q61171. MOUSE. THIOREDOXIN PEROXIDASE 1 (THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE 1) (THIOL-SPECIFIC ANTIOXIDANT PROTEIN) (TSA)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
789.3972 789.4107 -17.0966 151 157 (R)(L)
924.4299 924.4216 9.0519 192 198 (K)(-)
1211.6793 1211.6748 3.6817 140 150 (R)(S)
2465.2934 2465.2312 25.2133 1 26 (-)ASGNAQIGKSAPDFTATAVVDGAFK(E) Acet N
2713.4736 2713.3772 35.5328 68 91 (K)LGCEVLGVSVDSQFTHLAWINTPR(K) 1Cys-am
2841.5820 2841.4722 38.6458 67 91 (R)KLGCEVLGVSVDSQFTHLAWINTPR(K) 1Cys-am
2841.5820 2841.4722 38.6458 68 92 (K)LGCEVLGVSVDSQFTHLAWINTPRK(E) 1Cys-am

17 unmatched masses: 541.1996 568.1134 656.0546 825.1044 1179.6582 1194.6319 1296.7079 1465.7823 1475.7621 1567.7498 1716.9434 1735.0061 1863.1072 1868.0507 1885.1214 1941.0228 2384.0225

The matched peptides cover 38% (76/198 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/23 matches (17%). 72258.3 Da, pI = 5.94. Acc. # P35492. MOUSE. HISTIDINE AMMONIA-LYASE (EC 4.3.1.3) (HISTIDASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
789.3972 789.4358 -48.9569 572 578 (K)(V)
1211.6793 1211.7112 -26.3466 183 192 (K)(S)
1941.0228 1941.0446 -11.2275 452 469 (K)(R)
2465.2934 2465.3233 -12.1391 193 214 (R)SHSSGVGKPLSPERCRMLLALR(I) 1Cys-am

19 unmatched masses: 541.1996 568.1134 656.0546 825.1044 924.4299 1179.6582 1194.6319 1296.7079 1465.7823 1475.7621 1567.7498 1716.9434 1735.0061 1863.1072 1868.0507 1885.1214 2384.0225 2713.4736 2841.5820

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
789.3972	789.4358	-48.9569	572	578	(K)(V)
1211.6793	1211.7112	-26.3466	183	192	(K)(S)
1941.0228	1941.0446	-11.2275	452	469	(K)(R)
2465.2934	2465.3233	-12.1391	193	214	(R)SHSSGVGKPLSPERCRMLLALR(I)	1Cys-am

The matched peptides cover 8% (57/657 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/23 matches (17%). 67183.4 Da, pI = 5.54. Acc. # P32456. HUMAN. INTERFERON-INDUCED GUANYLATE-BINDING PROTEIN 2 (GUANINE NUCLEOTIDE- BINDING PROTEIN 2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1465.7823 1465.7666 10.6819 232 243 (R)(Y)
1868.0507 1868.0005 26.8688 216 230 (K)(R)
1941.0228 1940.9825 20.7182 1 18 (-)(G)
2384.0225 2384.0824 -25.1264 400 421 (K)(Q)

19 unmatched masses: 541.1996 568.1134 656.0546 789.3972 825.1044 924.4299 1179.6582 1194.6319 1211.6793 1296.7079 1475.7621 1567.7498 1716.9434 1735.0061 1863.1072 1885.1214 2465.2934 2713.4736 2841.5820

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1465.7823	1465.7666	10.6819	232	243	(R)(Y)
1868.0507	1868.0005	26.8688	216	230	(K)(R)
1941.0228	1940.9825	20.7182	1	18	(-)(G)
2384.0225	2384.0824	-25.1264	400	421	(K)(Q)

The matched peptides cover 11% (67/591 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/23 matches (21%). 78944.9 Da, pI = 4.61. Acc. # Q06335. MOUSE. AMYLOID-LIKE PROTEIN 2 PRECURSOR (CDEI-BOX BINDING PROTEIN) (CDEBP)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
924.4299 924.4501 -21.8024 689 695 (K)(-)
1194.6319 1194.6595 -23.1398 394 403 (K)(V)
1211.6793 1211.6544 20.5546 345 353 (R)(K)
1567.7498 1567.7968 -29.9412 603 617 (R)(E)
1885.1214 1885.0370 44.7741 372 387 (R)(E)

18 unmatched masses: 541.1996 568.1134 656.0546 789.3972 825.1044 1179.6582 1296.7079 1465.7823 1475.7621 1716.9434 1735.0061 1863.1072 1868.0507 1941.0228 2384.0225 2465.2934 2713.4736 2841.5820

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
924.4299	924.4501	-21.8024	689	695	(K)(-)
1194.6319	1194.6595	-23.1398	394	403	(K)(V)
1211.6793	1211.6544	20.5546	345	353	(R)(K)
1567.7498	1567.7968	-29.9412	603	617	(R)(E)
1885.1214	1885.0370	44.7741	372	387	(R)(E)

The matched peptides cover 8% (56/695 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):