MS-Fit Search Results

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Sample ID (comment): HSG017
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 487 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
29

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.36e+004	11/29 (37%)	17148.8 / 5.83	HUMAN	NUCLEOSIDE DIPHOSPHATE KINASE A (EC 2.7.4.6) (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NM23-H1).
2	9.48e+003	8/29 (27%)	17363.2 / 6.97	MOUSE	NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-M2) (P18).
3	3.68e+003	9/29 (31%)	17207.9 / 6.84	MOUSE	NUCLEOSIDE DIPHOSPHATE KINASE A (EC 2.7.4.6) (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23).
4	2.58e+003	7/29 (24%)	17298.1 / 8.52	HUMAN	NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF).
5	582	5/29 (17%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

Detailed Results

1. 11/29 matches (37%). 17148.8 Da, pI = 5.83. Acc. # P15531. HUMAN. NUCLEOSIDE DIPHOSPHATE KINASE A (EC 2.7.4.6) (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23) (NM23-H1)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
984.6450	984.6206	24.7949	19	27	(R)(F)
1065.5453	1065.5151	28.2679	106	114	(R)GDFCIQVGR(N)	1Cys-am
1149.6875	1149.6421	39.5608	57	66	(K)(Y)
1344.8191	1344.7640	40.9876	7	18	(R)(G)
1485.7692	1485.7185	34.0856	115	128	(R)(E)
1785.9803	1785.9169	35.4705	89	105	(R)(G)
1801.9633	1801.9118	28.5473	89	105	(R)VMLGETNPADSKPGTIR(G)	1Met-ox
2034.1956	2034.0813	56.2191	50	66	(K)(Y)
2082.1160	2082.0218	45.2725	40	56	(K)FMQASEDLLKEHYVDLK(D)	1Met-ox
2132.2068	2132.0673	65.4294	67	85	(K)YMHSGPVVAMVWEGLNVVK(T)	1Met-ox
2148.1812	2148.0622	55.3840	67	85	(K)YMHSGPVVAMVWEGLNVVK(T)	2Met-ox

18 unmatched masses: 541.2306 568.1467 647.2583 842.5274 1151.2324 1179.6426 1267.7625 1383.7796 1475.8302 1760.0106 1941.0327 1994.0851 2164.1831 2202.2746 2284.2840 2299.2776 2384.1297 2705.3343

The matched peptides cover 70% (107/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/29 matches (27%). 17363.2 Da, pI = 6.97. Acc. # Q01768. MOUSE. NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-M2) (P18)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
984.6450 984.6206 24.7949 19 27 (R)(F)
1065.5453 1065.5151 28.2679 106 114 (R)GDFCIQVGR(N) 1Cys-am
1344.8191 1344.7640 40.9876 7 18 (R)(G)
1485.7692 1485.7185 34.0856 115 128 (R)(E)
1785.9803 1785.9169 35.4705 89 105 (R)(G)
1801.9633 1801.9118 28.5473 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2132.2068 2132.1803 12.4303 32 49 (K)(Q)
2148.1812 2148.1752 2.7800 32 49 (K)GFRLVAMKFLRASEEHLK(Q) 1Met-ox

21 unmatched masses: 541.2306 568.1467 647.2583 842.5274 1149.6875 1151.2324 1179.6426 1267.7625 1383.7796 1475.8302 1760.0106 1941.0327 1994.0851 2034.1956 2082.1160 2164.1831 2202.2746 2284.2840 2299.2776 2384.1297 2705.3343

The matched peptides cover 51% (79/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/29 matches (31%). 17207.9 Da, pI = 6.84. Acc. # P15532. MOUSE. NUCLEOSIDE DIPHOSPHATE KINASE A (EC 2.7.4.6) (NDK A) (NDP KINASE A) (TUMOR METASTATIC PROCESS-ASSOCIATED PROTEIN) (METASTASIS INHIBITION FACTOR NM23)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
984.6450 984.6206 24.7949 19 27 (R)(F)
1065.5453 1065.5151 28.2679 106 114 (R)GDFCIQVGR(N) 1Cys-am
1179.6426 1179.6526 -8.4761 57 66 (K)(Y)
1344.8191 1344.7640 40.9876 7 18 (R)(G)
1785.9803 1785.9169 35.4705 89 105 (R)(G)
1801.9633 1801.9118 28.5473 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2034.1956 2034.1752 10.0550 32 49 (K)(E)
2132.2068 2132.0673 65.4294 67 85 (K)YMHSGPVVAMVWEGLNVVK(T) 1Met-ox
2148.1812 2148.0622 55.3840 67 85 (K)YMHSGPVVAMVWEGLNVVK(T) 2Met-ox

20 unmatched masses: 541.2306 568.1467 647.2583 842.5274 1149.6875 1151.2324 1267.7625 1383.7796 1475.8302 1485.7692 1760.0106 1941.0327 1994.0851 2082.1160 2164.1831 2202.2746 2284.2840 2299.2776 2384.1297 2705.3343

The matched peptides cover 61% (94/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/29 matches (24%). 17298.1 Da, pI = 8.52. Acc. # P22392. HUMAN. NUCLEOSIDE DIPHOSPHATE KINASE B (EC 2.7.4.6) (NDK B) (NDP KINASE B) (NM23-H2) (C-MYC PURINE-BINDING TRANSCRIPTION FACTOR PUF)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
984.6450 984.6206 24.7949 19 27 (R)(F)
1065.5453 1065.5151 28.2679 106 114 (R)GDFCIQVGR(N) 1Cys-am
1344.8191 1344.7640 40.9876 7 18 (R)(G)
1785.9803 1785.9169 35.4705 89 105 (R)(G)
1801.9633 1801.9118 28.5473 89 105 (R)VMLGETNPADSKPGTIR(G) 1Met-ox
2132.2068 2132.1803 12.4303 32 49 (K)(Q)
2148.1812 2148.1752 2.7800 32 49 (K)GFRLVAMKFLRASEEHLK(Q) 1Met-ox

22 unmatched masses: 541.2306 568.1467 647.2583 842.5274 1149.6875 1151.2324 1179.6426 1267.7625 1383.7796 1475.8302 1485.7692 1760.0106 1941.0327 1994.0851 2034.1956 2082.1160 2164.1831 2202.2746 2284.2840 2299.2776 2384.1297 2705.3343

The matched peptides cover 42% (65/152 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/29 matches (17%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6426 1179.6010 35.3024 376 385 (K)(H)
1383.7796 1383.6909 64.1242 185 196 (K)(V)
1475.8302 1475.7858 30.0741 199 210 (R)(W)
1475.8302 1475.7494 54.7305 211 222 (K)(T)
1994.0851 1993.9772 54.1216 223 238 (R)(R)
2384.1297 2383.9524 74.3708 518 548 (R)(G)

24 unmatched masses: 541.2306 568.1467 647.2583 842.5274 984.6450 1065.5453 1149.6875 1151.2324 1267.7625 1344.8191 1485.7692 1760.0106 1785.9803 1801.9633 1941.0327 2034.1956 2082.1160 2132.2068 2148.1812 2164.1831 2202.2746 2284.2840 2299.2776 2705.3343

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6426	1179.6010	35.3024	376	385	(K)(H)
1383.7796	1383.6909	64.1242	185	196	(K)(V)
1475.8302	1475.7858	30.0741	199	210	(R)(W)
1475.8302	1475.7494	54.7305	211	222	(K)(T)
1994.0851	1993.9772	54.1216	223	238	(R)(R)
2384.1297	2383.9524	74.3708	518	548	(R)(G)

The matched peptides cover 14% (93/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):