MS-Fit Search Results

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Sample ID (comment): HSG018
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 160 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
21

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.31e+003	10/21 (47%)	47038.0 / 6.99	HUMAN	ALPHA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON- NEURAL ENOLASE) (NNE) (PHOSPHOPYRUVATE HYDRATASE).
2	281	5/21 (23%)	23732.6 / 5.19	HUMAN	GS1 PROTEIN.
3	216	5/21 (23%)	46993.8 / 6.36	MOUSE	ALPHA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON- NEURAL ENOLASE) (NNE).
4	170	5/21 (23%)	49477.6 / 5.79	HUMAN	ALPHA ENOLASE, LUNG SPECIFIC (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON-NEURAL ENOLASE) (NNE) (PHOSPHOPYRUVATE HYDRATASE).
5	53.6	4/21 (19%)	70581.3 / 8.74	HUMAN	5-AMINOLEVULINIC ACID SYNTHASE MITOCHONDRIAL PRECURSOR, NONSPECIFIC (EC 2.3.1.37) (DELTA-AMINOLEVULINATE SYNTHASE) (DELTA-ALA SYNTHETASE) (ALAS-H).

Detailed Results

1. 10/21 matches (47%). 47038.0 Da, pI = 6.99. Acc. # P06733. HUMAN. ALPHA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON- NEURAL ENOLASE) (NNE) (PHOSPHOPYRUVATE HYDRATASE)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
704.4583	704.4095	69.2453	126	131	(K)(H)
766.4270	766.3735	69.7201	9	14	(R)(G)
806.5100	806.4525	71.3118	406	411	(K)(I)
959.5783	959.5427	37.0964	426	433	(R)(-)
1143.6764	1143.6162	52.5820	183	192	(R)(N)
1540.8499	1540.7834	43.1708	239	252	(K)(S)
1556.8727	1556.7783	60.6343	239	252	(K)VVIGMDVAASEFFR(S)	1Met-ox
1805.0020	1804.9445	31.8693	32	49	(R)(D)
1924.0431	1923.9825	31.5285	162	178	(K)LAMQEFMILPVGAANFR(E)	1Met-ox
1940.0398	1939.9774	32.1811	162	178	(K)LAMQEFMILPVGAANFR(E)	2Met-ox

11 unmatched masses: 804.3384 857.6021 915.5222 1003.5807 1047.6315 1091.6419 1117.1701 1179.7228 1296.7638 1879.2313 3012.5849

The matched peptides cover 19% (84/433 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/21 matches (23%). 23732.6 Da, pI = 5.19. Acc. # Q08623. HUMAN. GS1 PROTEIN..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1003.5807 1003.4923 88.1079 148 156 (R)(C)
1540.8499 1540.8198 19.5554 26 38 (K)(K)
1540.8499 1540.8198 19.5554 27 39 (K)(A)
1556.8727 1556.8147 37.2611 26 38 (K)KYSWDVKSLVMGK(K) 1Met-ox
1556.8727 1556.8147 37.2611 27 39 (K)YSWDVKSLVMGKK(A) 1Met-ox
1924.0431 1923.9275 60.1232 91 109 (K)(T)
1940.0398 1939.9224 60.5401 91 109 (K)HGIPFALATSSGSASFDMK(T) 1Met-ox

16 unmatched masses: 704.4583 766.4270 804.3384 806.5100 857.6021 915.5222 959.5783 1047.6315 1091.6419 1117.1701 1143.6764 1179.7228 1296.7638 1805.0020 1879.2313 3012.5849

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1003.5807	1003.4923	88.1079	148	156	(R)(C)
1540.8499	1540.8198	19.5554	26	38	(K)(K)
1540.8499	1540.8198	19.5554	27	39	(K)(A)
1556.8727	1556.8147	37.2611	26	38	(K)KYSWDVKSLVMGK(K)	1Met-ox
1556.8727	1556.8147	37.2611	27	39	(K)YSWDVKSLVMGKK(A)	1Met-ox
1924.0431	1923.9275	60.1232	91	109	(K)(T)
1940.0398	1939.9224	60.5401	91	109	(K)HGIPFALATSSGSASFDMK(T)	1Met-ox

The matched peptides cover 19% (42/214 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/21 matches (23%). 46993.8 Da, pI = 6.36. Acc. # P17182. MOUSE. ALPHA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON- NEURAL ENOLASE) (NNE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
704.4583 704.4095 69.2453 126 131 (K)(H)
766.4270 766.3735 69.7201 9 14 (R)(G)
806.5100 806.4525 71.3118 406 411 (K)(I)
1143.6764 1143.6162 52.5820 183 192 (R)(N)
1805.0020 1804.9445 31.8693 32 49 (R)(D)

16 unmatched masses: 804.3384 857.6021 915.5222 959.5783 1003.5807 1047.6315 1091.6419 1117.1701 1179.7228 1296.7638 1540.8499 1556.8727 1879.2313 1924.0431 1940.0398 3012.5849

The matched peptides cover 10% (46/433 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/21 matches (23%). 49477.6 Da, pI = 5.79. Acc. # Q05524. HUMAN. ALPHA ENOLASE, LUNG SPECIFIC (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NON-NEURAL ENOLASE) (NNE) (PHOSPHOPYRUVATE HYDRATASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
704.4583 704.4095 69.2453 135 140 (K)(H)
766.4270 766.3735 69.7201 11 16 (R)(G)
806.5100 806.4525 71.3118 430 435 (K)(I)
1143.6764 1143.6162 52.5820 193 202 (R)(N)
1556.8727 1556.7783 60.6343 249 262 (K)(D)

16 unmatched masses: 804.3384 857.6021 915.5222 959.5783 1003.5807 1047.6315 1091.6419 1117.1701 1179.7228 1296.7638 1540.8499 1805.0020 1879.2313 1924.0431 1940.0398 3012.5849

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
704.4583	704.4095	69.2453	135	140	(K)(H)
766.4270	766.3735	69.7201	11	16	(R)(G)
806.5100	806.4525	71.3118	430	435	(K)(I)
1143.6764	1143.6162	52.5820	193	202	(R)(N)
1556.8727	1556.7783	60.6343	249	262	(K)(D)

The matched peptides cover 9% (42/458 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/21 matches (19%). 70581.3 Da, pI = 8.74. Acc. # P13196. HUMAN. 5-AMINOLEVULINIC ACID SYNTHASE MITOCHONDRIAL PRECURSOR, NONSPECIFIC (EC 2.3.1.37) (DELTA-AMINOLEVULINATE SYNTHASE) (DELTA-ALA SYNTHETASE) (ALAS-H)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
915.5222 915.4688 58.2633 289 295 (K)(E)
1556.8727 1556.8086 41.1514 354 365 (K)(E)
1924.0431 1923.9162 65.9624 226 242 (R)(K)
1940.0398 1939.9112 66.3311 226 242 (R)AHIFPMADDYSDSLITK(K) 1Met-ox

17 unmatched masses: 704.4583 766.4270 804.3384 806.5100 857.6021 959.5783 1003.5807 1047.6315 1091.6419 1117.1701 1143.6764 1179.7228 1296.7638 1540.8499 1805.0020 1879.2313 3012.5849

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
915.5222	915.4688	58.2633	289	295	(K)(E)
1556.8727	1556.8086	41.1514	354	365	(K)(E)
1924.0431	1923.9162	65.9624	226	242	(R)(K)
1940.0398	1939.9112	66.3311	226	242	(R)AHIFPMADDYSDSLITK(K)	1Met-ox

The matched peptides cover 5% (36/640 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):