MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG022
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 573 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
50

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.41e+004	10/50 (20%)	27774.2 / 4.68	HUMAN	14-3-3 PROTEIN SIGMA (STRATIFIN) (EPITHELIAL CELL MARKER PROTEIN 1).
2	5.21e+003	7/50 (14%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	1.18e+003	8/50 (16%)	82945.1 / 8.49	MOUSE	HEPATOCYTE GROWTH FACTOR PRECURSOR (SCATTER FACTOR) (SF) (HEPATOPOEITIN A).
4	776	5/50 (10%)	27764.4 / 4.68	HUMAN	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN).
5	268	5/50 (10%)	30674.5 / 9.45	MOUSE	P4-6 PROTEIN.

Detailed Results

1. 10/50 matches (20%). 27774.2 Da, pI = 4.68. Acc. # P31947. HUMAN. 14-3-3 PROTEIN SIGMA (STRATIFIN) (EPITHELIAL CELL MARKER PROTEIN 1)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
903.5524	903.5151	41.2419	61	68	(R)(S)
1070.5563	1070.5305	24.1172	161	169	(K)EMPPTNPIR(L)	1Met-ox
1189.6652	1189.6615	3.1109	215	224	(K)(D)
1205.6918	1205.6564	29.4083	215	224	(K)DSTLIMQLLR(D)	1Met-ox
1546.7593	1546.6985	39.2926	69	82	(K)(E)
1606.7772	1606.7383	24.2220	28	41	(K)GAVEKGEELSCEER(N)	1Cys-am
1995.0040	1994.9056	49.3277	69	85	(K)(E)
2173.9259	2173.9600	-15.6794	196	214	(K)(D)
2189.9770	2189.9549	10.0874	196	214	(K)TTFDEAMADLHTLSEDSYK(D)	1Met-ox
2833.5388	2833.4776	21.5771	170	195	(R)(T)

40 unmatched masses: 523.1982 541.2157 568.1620 585.3471 588.2905 602.2724 656.0711 814.4894 842.5455 1145.3984 1179.6600 1188.6458 1277.7343 1302.7688 1323.6842 1475.7997 1497.8142 1532.7803 1566.7329 1707.8053 1716.8796 1791.7007 1837.9399 1940.9781 1994.0197 2014.9457 2083.0725 2142.0110 2144.0342 2211.1176 2299.1974 2384.0144 2385.9600 2530.1693 2705.2320 2706.1814 2717.2368 2718.0509 2836.4628 2856.6150

The matched peptides cover 41% (103/248 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/50 matches (14%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1277.7343 1277.7105 18.5991 472 482 (K)(T)
1302.7688 1302.7157 40.7996 343 354 (R)(A)
1475.7997 1475.7858 9.4024 199 210 (R)(W)
1475.7997 1475.7494 34.0583 211 222 (K)(T)
1716.8796 1716.8517 16.2618 417 431 (K)(G)
1994.0197 1993.9772 21.2888 223 238 (R)(R)
2384.0144 2383.9524 26.0258 518 548 (R)(G)
2530.1693 2530.2612 -36.3134 276 297 (K)(V)

43 unmatched masses: 523.1982 541.2157 568.1620 585.3471 588.2905 602.2724 656.0711 814.4894 842.5455 903.5524 1070.5563 1145.3984 1179.6600 1188.6458 1189.6652 1205.6918 1323.6842 1497.8142 1532.7803 1546.7593 1566.7329 1606.7772 1707.8053 1791.7007 1837.9399 1940.9781 1995.0040 2014.9457 2083.0725 2142.0110 2144.0342 2173.9259 2189.9770 2211.1176 2299.1974 2385.9600 2705.2320 2706.1814 2717.2368 2718.0509 2833.5388 2836.4628 2856.6150

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1277.7343	1277.7105	18.5991	472	482	(K)(T)
1302.7688	1302.7157	40.7996	343	354	(R)(A)
1475.7997	1475.7858	9.4024	199	210	(R)(W)
1475.7997	1475.7494	34.0583	211	222	(K)(T)
1716.8796	1716.8517	16.2618	417	431	(K)(G)
1994.0197	1993.9772	21.2888	223	238	(R)(R)
2384.0144	2383.9524	26.0258	518	548	(R)(G)
2530.1693	2530.2612	-36.3134	276	297	(K)(V)

The matched peptides cover 20% (131/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/50 matches (16%). 82945.1 Da, pI = 8.49. Acc. # Q08048. MOUSE. HEPATOCYTE GROWTH FACTOR PRECURSOR (SCATTER FACTOR) (SF) (HEPATOPOEITIN A)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1302.7688 1302.7170 39.7729 38 48 (R)(T)
1546.7593 1546.7436 10.1228 316 329 (R)(W)
1940.9781 1941.0380 -30.8592 518 534 (K)HICGGSLIKESWVLTAR(Q) 1Cys-am
2142.0110 2142.0045 3.0560 330 346 (R)(C)
2211.1176 2211.2171 -45.0198 128 149 (R)(C)
2299.1974 2299.2913 -40.8562 566 586 (K)(L)
2706.1814 2706.2074 -9.6175 375 398 (R)VGYCSQIPKCDVSSGQDCYRGNGK(N) 2Cys-am
2833.5388 2833.4810 20.3875 587 612 (K)(T)

42 unmatched masses: 523.1982 541.2157 568.1620 585.3471 588.2905 602.2724 656.0711 814.4894 842.5455 903.5524 1070.5563 1145.3984 1179.6600 1188.6458 1189.6652 1205.6918 1277.7343 1323.6842 1475.7997 1497.8142 1532.7803 1566.7329 1606.7772 1707.8053 1716.8796 1791.7007 1837.9399 1994.0197 1995.0040 2014.9457 2083.0725 2144.0342 2173.9259 2189.9770 2384.0144 2385.9600 2530.1693 2705.2320 2717.2368 2718.0509 2836.4628 2856.6150

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1302.7688	1302.7170	39.7729	38	48	(R)(T)
1546.7593	1546.7436	10.1228	316	329	(R)(W)
1940.9781	1941.0380	-30.8592	518	534	(K)HICGGSLIKESWVLTAR(Q)	1Cys-am
2142.0110	2142.0045	3.0560	330	346	(R)(C)
2211.1176	2211.2171	-45.0198	128	149	(R)(C)
2299.1974	2299.2913	-40.8562	566	586	(K)(L)
2706.1814	2706.2074	-9.6175	375	398	(R)VGYCSQIPKCDVSSGQDCYRGNGK(N)	2Cys-am
2833.5388	2833.4810	20.3875	587	612	(K)(T)

The matched peptides cover 20% (152/728 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/50 matches (10%). 27764.4 Da, pI = 4.68. Acc. # P27348. HUMAN. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
903.5524 903.5151 41.2419 61 68 (R)(T)
1189.6652 1189.6615 3.1109 213 222 (K)(D)
1205.6918 1205.6564 29.4083 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1532.7803 1532.7193 39.8275 28 41 (K)(N)
2014.9457 2014.9358 4.9180 140 157 (K)(K)

45 unmatched masses: 523.1982 541.2157 568.1620 585.3471 588.2905 602.2724 656.0711 814.4894 842.5455 1070.5563 1145.3984 1179.6600 1188.6458 1277.7343 1302.7688 1323.6842 1475.7997 1497.8142 1546.7593 1566.7329 1606.7772 1707.8053 1716.8796 1791.7007 1837.9399 1940.9781 1994.0197 1995.0040 2083.0725 2142.0110 2144.0342 2173.9259 2189.9770 2211.1176 2299.1974 2384.0144 2385.9600 2530.1693 2705.2320 2706.1814 2717.2368 2718.0509 2833.5388 2836.4628 2856.6150

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
903.5524	903.5151	41.2419	61	68	(R)(T)
1189.6652	1189.6615	3.1109	213	222	(K)(D)
1205.6918	1205.6564	29.4083	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1532.7803	1532.7193	39.8275	28	41	(K)(N)
2014.9457	2014.9358	4.9180	140	157	(K)(K)

The matched peptides cover 20% (50/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/50 matches (10%). 30674.5 Da, pI = 9.45. Acc. # P46686. MOUSE. P4-6 PROTEIN..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7997 1475.8078 -5.5033 149 161 (R)(Q)
1566.7329 1566.7811 -30.7661 86 99 (K)(S)
2144.0342 2144.0560 -10.2044 249 267 (R)YPLCPLQAFAICLSSFDGK(L) 1Cys-am
2705.2320 2705.3074 -27.8671 45 68 (K)(K)
2833.5388 2833.4023 48.1442 45 69 (K)(R)

45 unmatched masses: 523.1982 541.2157 568.1620 585.3471 588.2905 602.2724 656.0711 814.4894 842.5455 903.5524 1070.5563 1145.3984 1179.6600 1188.6458 1189.6652 1205.6918 1277.7343 1302.7688 1323.6842 1497.8142 1532.7803 1546.7593 1606.7772 1707.8053 1716.8796 1791.7007 1837.9399 1940.9781 1994.0197 1995.0040 2014.9457 2083.0725 2142.0110 2173.9259 2189.9770 2211.1176 2299.1974 2384.0144 2385.9600 2530.1693 2706.1814 2717.2368 2718.0509 2836.4628 2856.6150

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7997	1475.8078	-5.5033	149	161	(R)(Q)
1566.7329	1566.7811	-30.7661	86	99	(K)(S)
2144.0342	2144.0560	-10.2044	249	267	(R)YPLCPLQAFAICLSSFDGK(L)	1Cys-am
2705.2320	2705.3074	-27.8671	45	68	(K)(K)
2833.5388	2833.4023	48.1442	45	69	(K)(R)

The matched peptides cover 26% (71/271 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):