MS-Fit Search Results

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Sample ID (comment): HSG023
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 49 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.27e+005	10/20 (50%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).
2	3.75e+004	9/20 (45%)	27771.3 / 4.73	MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT).
3	301	6/20 (30%)	28087.7 / 4.76	HUMAN	14-3-3 PROTEIN ETA (PROTEIN AS1).
4	138	5/20 (25%)	27764.4 / 4.68	HUMAN	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN).
5	81.2	4/20 (20%)	27951.3 / 4.76	HUMAN	14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054).

Detailed Results

1. 10/20 matches (50%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3827	615.3578	40.3867	50	55	(K)(R)
669.4003	669.3976	4.1287	116	120	(K)(M)
1189.6884	1189.6615	22.6635	213	222	(K)(D)
1205.6816	1205.6564	20.9150	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1304.6970	1304.6850	9.1683	104	115	(K)(V)
1548.7471	1548.7142	21.2781	28	41	(K)(N)
2041.0296	2040.9878	20.4518	140	157	(K)(K)
2132.0395	2131.9923	22.1317	194	212	(K)(D)
2169.1394	2169.0828	26.1202	139	157	(K)(K)
2169.1394	2169.0828	26.1202	140	158	(K)(E)
2705.2361	2705.2868	-18.7366	188	212	(K)(D)

10 unmatched masses: 550.1335 568.1464 588.2660 656.0940 842.5302 1532.7687 1940.9895 1994.0264 2063.0189 2384.0477

The matched peptides cover 37% (92/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/20 matches (45%). 27771.3 Da, pI = 4.73. Acc. # P35215. MOUSE. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3827 615.3578 40.3867 50 55 (K)(R)
669.4003 669.3976 4.1287 116 120 (K)(M)
1189.6884 1189.6615 22.6635 213 222 (K)(D)
1205.6816 1205.6564 20.9150 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1548.7471 1548.7142 21.2781 28 41 (K)(N)
2041.0296 2040.9878 20.4518 140 157 (K)(K)
2132.0395 2131.9923 22.1317 194 212 (K)(D)
2169.1394 2169.0828 26.1202 139 157 (K)(K)
2169.1394 2169.0828 26.1202 140 158 (K)(E)
2705.2361 2705.2868 -18.7366 188 212 (K)(D)

11 unmatched masses: 550.1335 568.1464 588.2660 656.0940 842.5302 1304.6970 1532.7687 1940.9895 1994.0264 2063.0189 2384.0477

The matched peptides cover 32% (80/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/20 matches (30%). 28087.7 Da, pI = 4.76. Acc. # Q04917. HUMAN. 14-3-3 PROTEIN ETA (PROTEIN AS1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3827 615.3578 40.3867 50 55 (K)(R)
669.4003 669.3976 4.1287 120 124 (K)(M)
1189.6884 1189.6615 22.6635 217 226 (K)(D)
1205.6816 1205.6564 20.9150 217 226 (K)DSTLIMQLLR(D) 1Met-ox
2041.0296 2041.0177 5.8214 155 171 (K)(L)
2132.0395 2132.1161 -35.9155 88 105 (K)IEKELETVCNDVLSLLDK(F) 1Cys-am

14 unmatched masses: 550.1335 568.1464 588.2660 656.0940 842.5302 1304.6970 1532.7687 1548.7471 1940.9895 1994.0264 2063.0189 2169.1394 2384.0477 2705.2361

The matched peptides cover 22% (56/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/20 matches (25%). 27764.4 Da, pI = 4.68. Acc. # P27348. HUMAN. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
669.4003 669.3976 4.1287 116 120 (K)(M)
1189.6884 1189.6615 22.6635 213 222 (K)(D)
1205.6816 1205.6564 20.9150 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1532.7687 1532.7193 32.2390 28 41 (K)(N)
2132.0395 2132.1385 -46.4523 1 18 (-)MEKTELIQKAKLAEQAER(Y) 1Met-ox

15 unmatched masses: 550.1335 568.1464 588.2660 615.3827 656.0940 842.5302 1304.6970 1548.7471 1940.9895 1994.0264 2041.0296 2063.0189 2169.1394 2384.0477 2705.2361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
669.4003	669.3976	4.1287	116	120	(K)(M)
1189.6884	1189.6615	22.6635	213	222	(K)(D)
1205.6816	1205.6564	20.9150	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1532.7687	1532.7193	32.2390	28	41	(K)(N)
2132.0395	2132.1385	-46.4523	1	18	(-)MEKTELIQKAKLAEQAER(Y)	1Met-ox

The matched peptides cover 19% (47/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/20 matches (20%). 27951.3 Da, pI = 4.76. Acc. # P31946. HUMAN. 14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3827 615.3578 40.3867 51 56 (K)(R)
669.4003 669.3976 4.1287 117 121 (K)(M)
1189.6884 1189.6615 22.6635 214 223 (K)(D)
1205.6816 1205.6564 20.9150 214 223 (K)DSTLIMQLLR(D) 1Met-ox

16 unmatched masses: 550.1335 568.1464 588.2660 656.0940 842.5302 1304.6970 1532.7687 1548.7471 1940.9895 1994.0264 2041.0296 2063.0189 2132.0395 2169.1394 2384.0477 2705.2361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3827	615.3578	40.3867	51	56	(K)(R)
669.4003	669.3976	4.1287	117	121	(K)(M)
1189.6884	1189.6615	22.6635	214	223	(K)(D)
1205.6816	1205.6564	20.9150	214	223	(K)DSTLIMQLLR(D)	1Met-ox

The matched peptides cover 8% (21/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):