MS-Fit Search Results

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Sample ID (comment): HSG024
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 147 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
26

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.16e+004	9/26 (34%)	27951.3 / 4.76	HUMAN	14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054).
2	1.83e+004	11/26 (42%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).
3	1.83e+004	11/26 (42%)	27771.3 / 4.73	MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT).
4	1e+003	8/26 (30%)	28087.7 / 4.76	HUMAN	14-3-3 PROTEIN ETA (PROTEIN AS1).
5	493	6/26 (23%)	27764.4 / 4.68	HUMAN	14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN).

Detailed Results

1. 9/26 matches (34%). 27951.3 Da, pI = 4.76. Acc. # P31946. HUMAN. 14-3-3 PROTEIN BETA/ALPHA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (PROTEIN 1054)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3366	615.3578	-34.4461	51	56	(K)(R)
669.3725	669.3976	-37.4535	117	121	(K)(M)
816.4348	816.4216	16.2756	13	19	(K)(Y)
907.5025	907.5253	-25.0704	43	50	(R)(N)
1189.6492	1189.6615	-10.3341	214	223	(K)(D)
1205.6329	1205.6564	-19.4514	214	223	(K)DSTLIMQLLR(D)	1Met-ox
1598.7370	1598.7411	-2.5406	29	42	(K)(N)
2159.0065	2159.0257	-8.8897	140	158	(K)(K)
2159.0065	2159.0032	1.5163	195	213	(K)(D)
2287.0789	2287.1206	-18.2422	140	159	(K)(E)

17 unmatched masses: 612.2047 647.2259 691.3288 713.3526 842.5055 1156.5904 1548.7161 1643.7838 1940.9176 1993.9941 2040.9672 2130.9720 2142.9336 2169.0630 2283.1545 2299.1655 2717.0194

The matched peptides cover 36% (89/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/26 matches (42%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3366 615.3578 -34.4461 50 55 (K)(R)
669.3725 669.3976 -37.4535 116 120 (K)(M)
816.4348 816.4216 16.2756 12 18 (K)(Y)
907.5025 907.5253 -25.0704 42 49 (R)(N)
1189.6492 1189.6615 -10.3341 213 222 (K)(D)
1205.6329 1205.6564 -19.4514 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1548.7161 1548.7142 1.2441 28 41 (K)(N)
1598.7370 1598.8041 -41.9811 116 127 (K)VFYLKMKGDYYR(Y) 1Met-ox
2040.9672 2040.9878 -10.1211 140 157 (K)(K)
2130.9720 2130.9622 4.6093 10 27 (K)AKLAEQAERYDDMAACMK(S) 1Met-ox 1Cys-am
2169.0630 2169.0828 -9.1205 139 157 (K)(K)
2169.0630 2169.0828 -9.1205 140 158 (K)(E)

15 unmatched masses: 612.2047 647.2259 691.3288 713.3526 842.5055 1156.5904 1643.7838 1940.9176 1993.9941 2142.9336 2159.0065 2283.1545 2287.0789 2299.1655 2717.0194

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3366	615.3578	-34.4461	50	55	(K)(R)
669.3725	669.3976	-37.4535	116	120	(K)(M)
816.4348	816.4216	16.2756	12	18	(K)(Y)
907.5025	907.5253	-25.0704	42	49	(R)(N)
1189.6492	1189.6615	-10.3341	213	222	(K)(D)
1205.6329	1205.6564	-19.4514	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1548.7161	1548.7142	1.2441	28	41	(K)(N)
1598.7370	1598.8041	-41.9811	116	127	(K)VFYLKMKGDYYR(Y)	1Met-ox
2040.9672	2040.9878	-10.1211	140	157	(K)(K)
2130.9720	2130.9622	4.6093	10	27	(K)AKLAEQAERYDDMAACMK(S)	1Met-ox 1Cys-am
2169.0630	2169.0828	-9.1205	139	157	(K)(K)
2169.0630	2169.0828	-9.1205	140	158	(K)(E)

The matched peptides cover 35% (88/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/26 matches (42%). 27771.3 Da, pI = 4.73. Acc. # P35215. MOUSE. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3366 615.3578 -34.4461 50 55 (K)(R)
669.3725 669.3976 -37.4535 116 120 (K)(M)
816.4348 816.4216 16.2756 12 18 (K)(Y)
907.5025 907.5253 -25.0704 42 49 (R)(N)
1189.6492 1189.6615 -10.3341 213 222 (K)(D)
1205.6329 1205.6564 -19.4514 213 222 (K)DSTLIMQLLR(D) 1Met-ox
1548.7161 1548.7142 1.2441 28 41 (K)(N)
1598.7370 1598.8041 -41.9811 116 127 (K)VFYLKMKGDYYR(Y) 1Met-ox
2040.9672 2040.9878 -10.1211 140 157 (K)(K)
2130.9720 2130.9622 4.6093 10 27 (K)AKLAEQAERYDDMAACMK(S) 1Met-ox 1Cys-am
2169.0630 2169.0828 -9.1205 139 157 (K)(K)
2169.0630 2169.0828 -9.1205 140 158 (K)(E)

15 unmatched masses: 612.2047 647.2259 691.3288 713.3526 842.5055 1156.5904 1643.7838 1940.9176 1993.9941 2142.9336 2159.0065 2283.1545 2287.0789 2299.1655 2717.0194

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3366	615.3578	-34.4461	50	55	(K)(R)
669.3725	669.3976	-37.4535	116	120	(K)(M)
816.4348	816.4216	16.2756	12	18	(K)(Y)
907.5025	907.5253	-25.0704	42	49	(R)(N)
1189.6492	1189.6615	-10.3341	213	222	(K)(D)
1205.6329	1205.6564	-19.4514	213	222	(K)DSTLIMQLLR(D)	1Met-ox
1548.7161	1548.7142	1.2441	28	41	(K)(N)
1598.7370	1598.8041	-41.9811	116	127	(K)VFYLKMKGDYYR(Y)	1Met-ox
2040.9672	2040.9878	-10.1211	140	157	(K)(K)
2130.9720	2130.9622	4.6093	10	27	(K)AKLAEQAERYDDMAACMK(S)	1Met-ox 1Cys-am
2169.0630	2169.0828	-9.1205	139	157	(K)(K)
2169.0630	2169.0828	-9.1205	140	158	(K)(E)

The matched peptides cover 35% (88/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/26 matches (30%). 28087.7 Da, pI = 4.76. Acc. # Q04917. HUMAN. 14-3-3 PROTEIN ETA (PROTEIN AS1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3366 615.3578 -34.4461 50 55 (K)(R)
669.3725 669.3976 -37.4535 120 124 (K)(M)
816.4348 816.4216 16.2756 12 18 (R)(Y)
907.5025 907.5253 -25.0704 42 49 (R)(N)
1189.6492 1189.6615 -10.3341 217 226 (K)(D)
1205.6329 1205.6564 -19.4514 217 226 (K)DSTLIMQLLR(D) 1Met-ox
1598.7370 1598.8041 -41.9811 120 131 (K)VFYLKMKGDYYR(Y) 1Met-ox
2040.9672 2041.0177 -24.7511 155 171 (K)(L)

18 unmatched masses: 612.2047 647.2259 691.3288 713.3526 842.5055 1156.5904 1548.7161 1643.7838 1940.9176 1993.9941 2130.9720 2142.9336 2159.0065 2169.0630 2283.1545 2287.0789 2299.1655 2717.0194

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
615.3366	615.3578	-34.4461	50	55	(K)(R)
669.3725	669.3976	-37.4535	120	124	(K)(M)
816.4348	816.4216	16.2756	12	18	(R)(Y)
907.5025	907.5253	-25.0704	42	49	(R)(N)
1189.6492	1189.6615	-10.3341	217	226	(K)(D)
1205.6329	1205.6564	-19.4514	217	226	(K)DSTLIMQLLR(D)	1Met-ox
1598.7370	1598.8041	-41.9811	120	131	(K)VFYLKMKGDYYR(Y)	1Met-ox
2040.9672	2041.0177	-24.7511	155	171	(K)(L)

The matched peptides cover 24% (60/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/26 matches (23%). 27764.4 Da, pI = 4.68. Acc. # P27348. HUMAN. 14-3-3 PROTEIN TAU (14-3-3 PROTEIN THETA) (14-3-3 PROTEIN T-CELL) (HS1 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
669.3725 669.3976 -37.4535 116 120 (K)(M)
816.4348 816.4216 16.2756 12 18 (K)(Y)
907.5025 907.5253 -25.0704 42 49 (R)(N)
1189.6492 1189.6615 -10.3341 213 222 (K)(D)
1205.6329 1205.6564 -19.4514 213 222 (K)DSTLIMQLLR(D) 1Met-ox
2142.9336 2143.0308 -45.3216 139 157 (R)(K)
2142.9336 2143.0308 -45.3216 140 158 (K)(E)

20 unmatched masses: 612.2047 615.3366 647.2259 691.3288 713.3526 842.5055 1156.5904 1548.7161 1598.7370 1643.7838 1940.9176 1993.9941 2040.9672 2130.9720 2159.0065 2169.0630 2283.1545 2287.0789 2299.1655 2717.0194

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
669.3725	669.3976	-37.4535	116	120	(K)(M)
816.4348	816.4216	16.2756	12	18	(K)(Y)
907.5025	907.5253	-25.0704	42	49	(R)(N)
1189.6492	1189.6615	-10.3341	213	222	(K)(D)
1205.6329	1205.6564	-19.4514	213	222	(K)DSTLIMQLLR(D)	1Met-ox
2142.9336	2143.0308	-45.3216	139	157	(R)(K)
2142.9336	2143.0308	-45.3216	140	158	(K)(E)

The matched peptides cover 20% (50/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):