MS-Fit Search Results

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Sample ID (comment): HSG025
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 62 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.11e+005	15/20 (75%)	61055.0 / 5.70	HUMAN	MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (P60 LYMPHOCYTE PROTEIN) (60 KD CHAPERONIN) (HEAT SHOCK PROTEIN 60) (HSP-60) (PROTEIN CPN60) (GROEL PROTEIN) (HUCHA60).
2	6.18e+003	9/20 (45%)	60955.8 / 5.91	MOUSE	MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (P60 LYMPHOCYTE PROTEIN) (60 KD CHAPERONIN) (HEAT SHOCK PROTEIN 60) (HSP-60) (PROTEIN CPN60) (GROEL PROTEIN) (HSP-65).
3	52.2	4/20 (20%)	69690.6 / 6.11	HUMAN	MERLIN (SCHWANNOMIN).
4	32.2	4/20 (20%)	41635.1 / 8.82	MOUSE	3 BETA-HYDROXYSTEROID DEHYDROGENASE TYPE IV (3BETA-HSD IV) (3-BETA- HYDROXY-DELTA(5)-STEROID DEHYDROGENASE) (EC 1.1.1.145) (3-BETA- HYDROXY-5-ENE STEROID DEHYDROGENASE) (PROGESTERONE REDUCTASE).
5	29.5	4/20 (20%)	44388.1 / 7.86	MOUSE	HOMEOBOX PROTEIN LIM-2 (LIM/HOMEODOMAIN PROTEIN LHX5).

Detailed Results

1. 15/20 matches (75%). 61055.0 Da, pI = 5.70. Acc. # P10809. HUMAN. MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (P60 LYMPHOCYTE PROTEIN) (60 KD CHAPERONIN) (HEAT SHOCK PROTEIN 60) (HSP-60) (PROTEIN CPN60) (GROEL PROTEIN) (HUCHA60)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1344.8251	1344.7163	80.8688	61	72	(R)(V)
1389.7852	1389.7054	57.3939	222	233	(R)(G)
1615.8939	1615.7678	78.0504	237	249	(K)CEFQDAYVLLSEK(K)	1Cys-am
1699.0539	1698.9213	78.0809	430	446	(R)AAVEEGIVLGGGCALLR(C)	1Cys-am
1919.2148	1919.0714	74.7229	251	268	(K)(K)
1939.0852	1938.9483	70.6402	206	221	(K)TLNDELEIIEGMKFDR(G)	1Met-ox
1968.2727	1968.1395	67.7069	291	309	(R)(K)
2038.1801	2038.0232	76.9768	371	387	(R)(E)
2047.3030	2047.1664	66.7286	250	268	(K)(K)
2145.2850	2145.1299	72.2786	38	58	(R)ALMLQGVDLLADAVAVTMGPK(G)	2Met-ox
2194.2820	2194.2395	19.3545	10	28	(R)(D)
2194.2820	2194.1243	71.8603	370	387	(K)(E)
2295.3433	2295.1608	79.5457	371	389	(R)(L)
2365.5182	2365.3343	77.7603	269	290	(R)(L)
2560.4178	2560.2491	65.8837	97	121	(K)(S)
3113.7300	3113.5101	70.6269	315	344	(K)DMAIATGGAVFGEEGLTLNLEDVQPHDLGK(V)	1Met-ox

5 unmatched masses: 568.1769 656.1111 1878.1534 1994.1398 2211.2524

The matched peptides cover 42% (245/573 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/20 matches (45%). 60955.8 Da, pI = 5.91. Acc. # P19226. MOUSE. MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (P60 LYMPHOCYTE PROTEIN) (60 KD CHAPERONIN) (HEAT SHOCK PROTEIN 60) (HSP-60) (PROTEIN CPN60) (GROEL PROTEIN) (HSP-65)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1344.8251 1344.7163 80.8688 61 72 (R)(V)
1389.7852 1389.7054 57.3939 222 233 (R)(G)
1615.8939 1615.7678 78.0504 237 249 (K)CEFQDAYVLLSEK(K) 1Cys-am
1699.0539 1698.9213 78.0809 430 446 (R)AAVEEGIVLGGGCALLR(C) 1Cys-am
1939.0852 1938.9483 70.6402 206 221 (K)TLNDELEIIEGMKFDR(G) 1Met-ox
1968.2727 1968.1395 67.7069 291 309 (R)(K)
2145.2850 2145.1299 72.2786 38 58 (R)ALMLQGVDLLADAVAVTMGPK(G) 2Met-ox
2365.5182 2365.3343 77.7603 269 290 (R)(L)
2560.4178 2560.2491 65.8837 97 121 (K)(S)

11 unmatched masses: 568.1769 656.1111 1878.1534 1919.2148 1994.1398 2038.1801 2047.3030 2194.2820 2211.2524 2295.3433 3113.7300

The matched peptides cover 27% (157/573 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 69690.6 Da, pI = 6.11. Acc. # P35240. HUMAN. MERLIN (SCHWANNOMIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1939.0852 1938.9094 90.6982 173 187 (R)(I)
2145.2850 2145.0803 95.4274 210 227 (K)(K)
2211.2524 2211.1450 48.5742 81 99 (K)(F)
2295.3433 2295.1985 63.1089 229 249 (K)(L)

16 unmatched masses: 568.1769 656.1111 1344.8251 1389.7852 1615.8939 1699.0539 1878.1534 1919.2148 1968.2727 1994.1398 2038.1801 2047.3030 2194.2820 2365.5182 2560.4178 3113.7300

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1939.0852	1938.9094	90.6982	173	187	(R)(I)
2145.2850	2145.0803	95.4274	210	227	(K)(K)
2211.2524	2211.1450	48.5742	81	99	(K)(F)
2295.3433	2295.1985	63.1089	229	249	(K)(L)

The matched peptides cover 12% (73/595 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 41635.1 Da, pI = 8.82. Acc. # Q61767. MOUSE. 3 BETA-HYDROXYSTEROID DEHYDROGENASE TYPE IV (3BETA-HSD IV) (3-BETA- HYDROXY-DELTA(5)-STEROID DEHYDROGENASE) (EC 1.1.1.145) (3-BETA- HYDROXY-5-ENE STEROID DEHYDROGENASE) (PROGESTERONE REDUCTASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1615.8939 1615.9093 -9.5352 55 69 (K)(R)
1939.0852 1939.0401 23.2593 33 48 (R)(L)
2145.2850 2145.1391 67.9896 1 20 (-)PGWSCLVTGAGGFLGQRIVR(M) 1Cys-am
2211.2524 2211.1014 68.2769 71 93 (R)(Q)

16 unmatched masses: 568.1769 656.1111 1344.8251 1389.7852 1699.0539 1878.1534 1919.2148 1968.2727 1994.1398 2038.1801 2047.3030 2194.2820 2295.3433 2365.5182 2560.4178 3113.7300

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1615.8939	1615.9093	-9.5352	55	69	(K)(R)
1939.0852	1939.0401	23.2593	33	48	(R)(L)
2145.2850	2145.1391	67.9896	1	20	(-)PGWSCLVTGAGGFLGQRIVR(M)	1Cys-am
2211.2524	2211.1014	68.2769	71	93	(R)(Q)

The matched peptides cover 19% (74/372 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/20 matches (20%). 44388.1 Da, pI = 7.86. Acc. # P50459. MOUSE. HOMEOBOX PROTEIN LIM-2 (LIM/HOMEODOMAIN PROTEIN LHX5)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1389.7852 1389.6796 75.9633 211 222 (R)(V)
2038.1801 2038.0214 77.8684 60 79 (R)(A)
2194.2820 2194.1225 72.6885 59 79 (R)(A)
2560.4178 2560.3095 42.3020 211 231 (R)(R)

16 unmatched masses: 568.1769 656.1111 1344.8251 1615.8939 1699.0539 1878.1534 1919.2148 1939.0852 1968.2727 1994.1398 2047.3030 2145.2850 2211.2524 2295.3433 2365.5182 3113.7300

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1389.7852	1389.6796	75.9633	211	222	(R)(V)
2038.1801	2038.0214	77.8684	60	79	(R)(A)
2194.2820	2194.1225	72.6885	59	79	(R)(A)
2560.4178	2560.3095	42.3020	211	231	(R)(R)

The matched peptides cover 10% (42/402 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):