MS-Fit Search Results

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Sample ID (comment): HSG027
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 314 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
30

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	456	6/30 (20%)	37374.9 / 5.85	HUMAN	FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).
2	247	5/30 (16%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	223	5/30 (16%)	77887.5 / 8.67	HUMAN	ZINC FINGER PROTEIN 7 (ZINC FINGER PROTEIN KOX4) (ZINC FINGER PROTEIN HF.16).
4	222	5/30 (16%)	39135.6 / 9.14	MOUSE	WNT-11 PROTEIN PRECURSOR.
4	222	6/30 (20%)	70438.6 / 9.20	MOUSE	ZINC FINGER PROTEIN ZFP-28 (MKR5 PROTEIN) (FRAGMENT).

Detailed Results

1. 6/30 matches (20%). 37374.9 Da, pI = 5.85. Acc. # P19883. HUMAN. FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1211.6908	1211.5036	154.4380	244	254	(K)SCEDIQCTGGK(K)	1Cys-am
1475.7794	1475.7130	44.9315	152	163	(K)(C)
1638.8654	1638.8638	1.0057	39	52	(K)(E)
1638.8654	1638.7038	98.6011	93	107	(K)(M)
1754.8561	1754.8239	18.3231	239	254	(K)CIKAKSCEDIQCTGGK(K)	1Cys-am
2299.2363	2299.1765	26.0150	6	25	(R)(S)
2705.1578	2705.3503	-71.1743	141	163	(R)(C)

24 unmatched masses: 523.1394 525.2339 538.1565 541.2010 568.1337 588.1735 650.0705 656.0750 672.0452 825.1106 841.0891 842.5221 861.0919 1179.5941 1359.8270 1563.7467 1566.7709 1755.8727 1940.9319 1983.0695 1994.0281 2211.1525 2233.0973 2384.0154

The matched peptides cover 26% (88/338 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/30 matches (16%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5941 1179.6010 -5.8709 376 385 (K)(H)
1475.7794 1475.7858 -4.3807 199 210 (R)(W)
1475.7794 1475.7494 20.2748 211 222 (K)(T)
1638.8654 1638.8604 3.0625 185 198 (K)(F)
1994.0281 1993.9772 25.5070 223 238 (R)(R)
2384.0154 2383.9524 26.4285 518 548 (R)(G)

25 unmatched masses: 523.1394 525.2339 538.1565 541.2010 568.1337 588.1735 650.0705 656.0750 672.0452 825.1106 841.0891 842.5221 861.0919 1211.6908 1359.8270 1563.7467 1566.7709 1754.8561 1755.8727 1940.9319 1983.0695 2211.1525 2233.0973 2299.2363 2705.1578

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5941	1179.6010	-5.8709	376	385	(K)(H)
1475.7794	1475.7858	-4.3807	199	210	(R)(W)
1475.7794	1475.7494	20.2748	211	222	(K)(T)
1638.8654	1638.8604	3.0625	185	198	(K)(F)
1994.0281	1993.9772	25.5070	223	238	(R)(R)
2384.0154	2383.9524	26.4285	518	548	(R)(G)

The matched peptides cover 14% (95/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/30 matches (16%). 77887.5 Da, pI = 8.67. Acc. # P17097. HUMAN. ZINC FINGER PROTEIN 7 (ZINC FINGER PROTEIN KOX4) (ZINC FINGER PROTEIN HF.16)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1359.8270 1359.6592 123.4386 327 337 (R)IHTGERPYGCR(E) 1Cys-am
1359.8270 1359.6479 131.7015 355 365 (R)THTGERPYPCK(E) 1Cys-am
1475.7794 1475.6557 83.7624 443 455 (K)CNKCTKAFGCSSR(L) 1Cys-am
1638.8654 1638.6991 101.4745 88 101 (R)(S)
2211.1525 2211.0756 34.7879 143 161 (K)VTGFTFQNNCLNEETVVPK(T) 1Cys-am
2233.0973 2232.9225 78.2767 240 257 (K)HTNNCHGEKPYECAECGK(V) 3Cys-am

25 unmatched masses: 523.1394 525.2339 538.1565 541.2010 568.1337 588.1735 650.0705 656.0750 672.0452 825.1106 841.0891 842.5221 861.0919 1179.5941 1211.6908 1563.7467 1566.7709 1754.8561 1755.8727 1940.9319 1983.0695 1994.0281 2299.2363 2384.0154 2705.1578

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1359.8270	1359.6592	123.4386	327	337	(R)IHTGERPYGCR(E)	1Cys-am
1359.8270	1359.6479	131.7015	355	365	(R)THTGERPYPCK(E)	1Cys-am
1475.7794	1475.6557	83.7624	443	455	(K)CNKCTKAFGCSSR(L)	1Cys-am
1638.8654	1638.6991	101.4745	88	101	(R)(S)
2211.1525	2211.0756	34.7879	143	161	(K)VTGFTFQNNCLNEETVVPK(T)	1Cys-am
2233.0973	2232.9225	78.2767	240	257	(K)HTNNCHGEKPYECAECGK(V)	3Cys-am

The matched peptides cover 12% (86/686 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/30 matches (16%). 39135.6 Da, pI = 9.14. Acc. # P48615. MOUSE. WNT-11 PROTEIN PRECURSOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7794 1475.6023 120.0165 333 342 (K)YHWCCYVTCR(R) 2Cys-am
1566.7709 1566.8062 -22.5228 33 46 (K)TPAALALNQTQHCK(Q) 1Cys-am
1754.8561 1755.0328 -100.7002 247 261 (K)(D)
1755.8727 1755.8198 30.1073 154 170 (R)(F)
2233.0973 2233.0157 36.5295 270 288 (K)(V)

25 unmatched masses: 523.1394 525.2339 538.1565 541.2010 568.1337 588.1735 650.0705 656.0750 672.0452 825.1106 841.0891 842.5221 861.0919 1179.5941 1211.6908 1359.8270 1563.7467 1638.8654 1940.9319 1983.0695 1994.0281 2211.1525 2299.2363 2384.0154 2705.1578

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7794	1475.6023	120.0165	333	342	(K)YHWCCYVTCR(R)	2Cys-am
1566.7709	1566.8062	-22.5228	33	46	(K)TPAALALNQTQHCK(Q)	1Cys-am
1754.8561	1755.0328	-100.7002	247	261	(K)(D)
1755.8727	1755.8198	30.1073	154	170	(R)(F)
2233.0973	2233.0157	36.5295	270	288	(K)(V)

The matched peptides cover 21% (75/354 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/30 matches (20%). 70438.6 Da, pI = 9.20. Acc. # P10078. MOUSE. ZINC FINGER PROTEIN ZFP-28 (MKR5 PROTEIN) (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5221 842.3757 173.8528 14 20 (R)(V)
1359.8270 1359.6731 113.2004 328 338 (R)IHTGEKPFECK(V) 1Cys-am
1563.7467 1563.8760 -82.6507 538 550 (R)(R)
1566.7709 1566.8141 -27.5409 427 439 (K)(V)
1754.8561 1754.7300 71.8368 496 510 (R)CHTGEKPYECSACGK(A) 2Cys-am
1994.0281 1994.0870 -29.5783 395 411 (K)(T)

24 unmatched masses: 523.1394 525.2339 538.1565 541.2010 568.1337 588.1735 650.0705 656.0750 672.0452 825.1106 841.0891 861.0919 1179.5941 1211.6908 1475.7794 1638.8654 1755.8727 1940.9319 1983.0695 2211.1525 2233.0973 2299.2363 2384.0154 2705.1578

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5221	842.3757	173.8528	14	20	(R)(V)
1359.8270	1359.6731	113.2004	328	338	(R)IHTGEKPFECK(V)	1Cys-am
1563.7467	1563.8760	-82.6507	538	550	(R)(R)
1566.7709	1566.8141	-27.5409	427	439	(K)(V)
1754.8561	1754.7300	71.8368	496	510	(R)CHTGEKPYECSACGK(A)	2Cys-am
1994.0281	1994.0870	-29.5783	395	411	(K)(T)

The matched peptides cover 12% (76/614 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):