MS-Fit Search Results

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Sample ID (comment): HSG028
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 173 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.66e+004	11/20 (55%)	37429.9 / 8.97	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS A2/B1 (HNRNP A2 AND HNRNP B1).
2	134	6/20 (30%)	58171.4 / 8.52	HUMAN	ZINC FINGER PROTEIN 35 (ZINC FINGER PROTEIN HF.10).
3	80.7	4/20 (20%)	75492.1 / 5.97	HUMAN	COAGULATION FACTOR XIII B CHAIN PRECURSOR (EC 2.3.2.13) (PROTEIN- GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE B CHAIN) (TRANSGLUTAMINASE B CHAIN).
4	66.3	4/20 (20%)	15585.3 / 9.84	HUMAN	MIDKINE PRECURSOR (NEURITE OUTGROWTH-PROMOTING PROTEIN) (MK) (MIDGESTATION AND KIDNEY PROTEIN) (AMPHIREGULIN-ASSOCIATED PROTEIN) (ARAP) (NEURITE OUTGROWTH-PROMOTING FACTOR 2).
5	51.9	5/20 (25%)	52106.9 / 8.19	HUMAN	NUCLEAR RECEPTOR ROR-BETA (NUCLEAR RECEPTOR RZR-BETA).

Detailed Results

1. 11/20 matches (55%). 37429.9 Da, pI = 8.97. Acc. # P22626. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS A2/B1 (HNRNP A2 AND HNRNP B1)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1013.4584	1013.4441	14.1659	204	213	(R)(G)
1050.4849	1050.4420	40.8562	130	137	(R)(I)
1087.5104	1087.4849	23.4478	39	46	(R)(L)
1377.6756	1377.6300	33.0822	214	228	(R)(G)
1695.8213	1695.7655	32.8922	154	168	(R)(I)
1798.9723	1798.9227	27.5629	23	38	(K)(N)
1880.0922	1879.9666	66.7772	114	129	(K)(D)
1927.1045	1927.0177	45.0496	22	38	(R)(N)
2205.9782	2205.9008	35.0924	326	350	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)	1Met-ox
2220.1935	2220.0712	55.0864	130	147	(R)(Q)
2495.1099	2495.0401	27.9958	239	266	(R)(G)

9 unmatched masses: 656.0795 825.1253 1613.9833 1615.9391 1763.8520 2097.0333 2145.0687 2211.1373 2498.1706

The matched peptides cover 43% (152/353 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/20 matches (30%). 58171.4 Da, pI = 8.52. Acc. # P13682. HUMAN. ZINC FINGER PROTEIN 35 (ZINC FINGER PROTEIN HF.10)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1615.9391 1615.8167 75.7382 390 402 (K)AFSCFSHLIVHQR(I) 1Cys-am
1695.8213 1695.6929 75.6812 325 337 (K)CYECNECGKTFTR(S) 2Cys-am
1695.8213 1695.7471 43.7494 375 389 (R)(A)
1763.8520 1763.7845 38.2676 459 473 (R)IHNGEKPYTCNECGK(A) 1Cys-am
1880.0922 1879.9046 99.7634 291 305 (K)IHSLEKTFKCNECEK(A) 1Cys-am
2220.1935 2220.0000 87.1679 316 333 (R)HQKVHITEKCYECNECGK(T) 1Cys-am
2498.1706 2498.2244 -21.5519 137 158 (K)ADPQGPELGEACEKGNMLKRQR(I) 1Cys-am

14 unmatched masses: 656.0795 825.1253 1013.4584 1050.4849 1087.5104 1377.6756 1613.9833 1798.9723 1927.1045 2097.0333 2145.0687 2205.9782 2211.1373 2495.1099

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1615.9391	1615.8167	75.7382	390	402	(K)AFSCFSHLIVHQR(I)	1Cys-am
1695.8213	1695.6929	75.6812	325	337	(K)CYECNECGKTFTR(S)	2Cys-am
1695.8213	1695.7471	43.7494	375	389	(R)(A)
1763.8520	1763.7845	38.2676	459	473	(R)IHNGEKPYTCNECGK(A)	1Cys-am
1880.0922	1879.9046	99.7634	291	305	(K)IHSLEKTFKCNECEK(A)	1Cys-am
2220.1935	2220.0000	87.1679	316	333	(R)HQKVHITEKCYECNECGK(T)	1Cys-am
2498.1706	2498.2244	-21.5519	137	158	(K)ADPQGPELGEACEKGNMLKRQR(I)	1Cys-am

The matched peptides cover 19% (102/519 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/20 matches (20%). 75492.1 Da, pI = 5.97. Acc. # P05160. HUMAN. COAGULATION FACTOR XIII B CHAIN PRECURSOR (EC 2.3.2.13) (PROTEIN- GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE B CHAIN) (TRANSGLUTAMINASE B CHAIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1615.9391 1615.7790 99.0865 321 333 (K)WTEPPKCIEGQEK(V) 1Cys-am
1927.1045 1926.9159 97.8633 192 207 (K)TEEVECLTYGWSLTPK(C) 1Cys-am
2097.0333 2097.0075 12.2877 334 352 (K)VACEEPPFIENGAANLHSK(I) 1Cys-am
2220.1935 2220.0971 43.4477 488 507 (K)(G)

16 unmatched masses: 656.0795 825.1253 1013.4584 1050.4849 1087.5104 1377.6756 1613.9833 1695.8213 1763.8520 1798.9723 1880.0922 2145.0687 2205.9782 2211.1373 2495.1099 2498.1706

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1615.9391	1615.7790	99.0865	321	333	(K)WTEPPKCIEGQEK(V)	1Cys-am
1927.1045	1926.9159	97.8633	192	207	(K)TEEVECLTYGWSLTPK(C)	1Cys-am
2097.0333	2097.0075	12.2877	334	352	(K)VACEEPPFIENGAANLHSK(I)	1Cys-am
2220.1935	2220.0971	43.4477	488	507	(K)(G)

The matched peptides cover 10% (68/661 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/20 matches (20%). 15585.3 Da, pI = 9.84. Acc. # P21741. HUMAN. MIDKINE PRECURSOR (NEURITE OUTGROWTH-PROMOTING PROTEIN) (MK) (MIDGESTATION AND KIDNEY PROTEIN) (AMPHIREGULIN-ASSOCIATED PROTEIN) (ARAP) (NEURITE OUTGROWTH-PROMOTING FACTOR 2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1050.4849 1050.4638 20.0884 58 67 (R)(I)
1695.8213 1695.7987 13.2954 72 85 (R)VPCNWKKEFGADCK(Y) 1Cys-am
2097.0333 2096.9832 23.8682 70 85 (R)CRVPCNWKKEFGADCK(Y) 3Cys-am
2205.9782 2205.9697 3.8437 30 50 (K)KGGPGSECAEWAWGPCTPSSK(D) 1Cys-am

16 unmatched masses: 656.0795 825.1253 1013.4584 1087.5104 1377.6756 1613.9833 1615.9391 1763.8520 1798.9723 1880.0922 1927.1045 2145.0687 2211.1373 2220.1935 2495.1099 2498.1706

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1050.4849	1050.4638	20.0884	58	67	(R)(I)
1695.8213	1695.7987	13.2954	72	85	(R)VPCNWKKEFGADCK(Y)	1Cys-am
2097.0333	2096.9832	23.8682	70	85	(R)CRVPCNWKKEFGADCK(Y)	3Cys-am
2205.9782	2205.9697	3.8437	30	50	(K)KGGPGSECAEWAWGPCTPSSK(D)	1Cys-am

The matched peptides cover 32% (47/143 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/20 matches (25%). 52106.9 Da, pI = 8.19. Acc. # Q92753. HUMAN. NUCLEAR RECEPTOR ROR-BETA (NUCLEAR RECEPTOR RZR-BETA)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1695.8213 1695.7835 22.2920 17 31 (K)SSGIHYGVITCEGCK(G) 2Cys-am
1798.9723 1798.9029 38.5468 57 69 (R)TNRNRCQHCRLQK(C) 2Cys-am
2205.9782 2206.1728 -88.1970 279 296 (K)RITGFMELCQNDQILLLK(S) 1Cys-am
2495.1099 2495.1632 -21.3446 37 56 (R)SQQNNASYSCPRQRNCLIDR(T) 2Cys-am
2498.1706 2498.3811 -84.2880 427 447 (K)(E)

15 unmatched masses: 656.0795 825.1253 1013.4584 1050.4849 1087.5104 1377.6756 1613.9833 1615.9391 1763.8520 1880.0922 1927.1045 2097.0333 2145.0687 2211.1373 2220.1935

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1695.8213	1695.7835	22.2920	17	31	(K)SSGIHYGVITCEGCK(G)	2Cys-am
1798.9723	1798.9029	38.5468	57	69	(R)TNRNRCQHCRLQK(C)	2Cys-am
2205.9782	2206.1728	-88.1970	279	296	(K)RITGFMELCQNDQILLLK(S)	1Cys-am
2495.1099	2495.1632	-21.3446	37	56	(R)SQQNNASYSCPRQRNCLIDR(T)	2Cys-am
2498.1706	2498.3811	-84.2880	427	447	(K)(E)

The matched peptides cover 18% (87/459 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):