MS-Fit Search Results

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Sample ID (comment): HSG030
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 267 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
22

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.31e+003	13/22 (59%)	29020.8 / 4.75	MOUSE	TROPOMYOSIN 5, CYTOSKELETAL TYPE.
2	2.31e+003	13/22 (59%)	29032.8 / 4.75	HUMAN	TROPOMYOSIN, CYTOSKELETAL TYPE (TM30-NM).
3	156	9/22 (40%)	32818.9 / 4.68	HUMAN	TROPOMYOSIN ALPHA CHAIN, SKELETAL MUSCLE TYPE.
4	123	7/22 (31%)	77094.8 / 6.53	HUMAN	PROPIONYL-COA CARBOXYLASE ALPHA CHAIN PRECURSOR (EC 6.4.1.3) (PCCASE) (PROPANOYL-COA:CARBON DIOXIDE LIGASE).
5	109	5/22 (22%)	51862.3 / 6.15	HUMAN	PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE).

Detailed Results

1. 13/22 matches (59%). 29020.8 Da, pI = 4.75. Acc. # P21107. MOUSE. TROPOMYOSIN 5, CYTOSKELETAL TYPE..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
722.3817	722.3473	47.6023	203	208	(R)(S)
1284.8097	1284.7527	44.3549	132	142	(R)(T)
1475.8201	1475.7780	28.5656	105	116	(K)MELQEIQLKEAK(H)	1Met-ox
1699.9109	1699.8840	15.8370	40	54	(R)(R)
1699.9109	1699.8840	15.8370	41	55	(R)(I)
1727.9426	1727.8928	28.8025	56	69	(R)(L)
1770.9487	1770.8986	28.2969	13	27	(R)(A)
1799.9990	1799.9867	6.8457	132	146	(R)(A)
1799.9990	1799.8486	83.6019	170	184	(K)CLSAAEEKYSQKEDK(Y)	1Cys-am
1815.9606	1815.8990	33.9709	117	131	(K)(K)
1884.0871	1883.9939	49.4569	55	69	(R)(L)
1999.0801	1998.9845	47.8348	14	30	(K)(L)
2127.1517	2127.0794	33.9762	13	30	(R)(L)
2202.2209	2202.1254	43.3943	70	89	(R)(G)
2556.4813	2556.3707	43.2646	93	113	(K)(E)
2556.4813	2556.3633	46.1569	132	153	(R)(C)

9 unmatched masses: 647.2992 656.0685 716.2445 842.5409 1638.9129 1962.0786 2211.2081 2384.0718 2500.3547

The matched peptides cover 60% (150/248 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/22 matches (59%). 29032.8 Da, pI = 4.75. Acc. # P12324. HUMAN. TROPOMYOSIN, CYTOSKELETAL TYPE (TM30-NM)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
722.3817 722.3473 47.6023 203 208 (R)(S)
1284.8097 1284.7527 44.3549 132 142 (R)(T)
1475.8201 1475.7780 28.5656 105 116 (K)MELQEIQLKEAK(H) 1Met-ox
1699.9109 1699.8840 15.8370 40 54 (R)(R)
1699.9109 1699.8840 15.8370 41 55 (R)(I)
1727.9426 1727.8928 28.8025 56 69 (R)(L)
1770.9487 1770.8986 28.2969 13 27 (R)(A)
1799.9990 1799.9867 6.8457 132 146 (R)(A)
1799.9990 1799.8486 83.6019 170 184 (K)CLSAAEEKYSQKEDK(Y) 1Cys-am
1815.9606 1815.8990 33.9709 117 131 (K)(K)
1884.0871 1883.9939 49.4569 55 69 (R)(L)
1999.0801 1998.9845 47.8348 14 30 (K)(L)
2127.1517 2127.0794 33.9762 13 30 (R)(L)
2202.2209 2202.1254 43.3943 70 89 (R)(G)
2556.4813 2556.3707 43.2646 93 113 (K)(E)
2556.4813 2556.3633 46.1569 132 153 (R)(C)

9 unmatched masses: 647.2992 656.0685 716.2445 842.5409 1638.9129 1962.0786 2211.2081 2384.0718 2500.3547

The matched peptides cover 60% (150/248 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/22 matches (40%). 32818.9 Da, pI = 4.68. Acc. # P06753. HUMAN. TROPOMYOSIN ALPHA CHAIN, SKELETAL MUSCLE TYPE..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
722.3817 722.3473 47.6023 239 244 (R)(S)
1284.8097 1284.7527 44.3549 168 178 (R)(T)
1475.8201 1475.7780 28.5656 141 152 (K)MELQEIQLKEAK(H) 1Met-ox
1727.9426 1727.8928 28.8025 92 105 (R)(L)
1799.9990 1799.9867 6.8457 168 182 (R)(A)
1815.9606 1815.8990 33.9709 153 167 (K)(K)
1884.0871 1883.9939 49.4569 91 105 (R)(L)
2202.2209 2202.1254 43.3943 106 125 (R)(G)
2556.4813 2556.3707 43.2646 129 149 (K)(E)

13 unmatched masses: 647.2992 656.0685 716.2445 842.5409 1638.9129 1699.9109 1770.9487 1962.0786 1999.0801 2127.1517 2211.2081 2384.0718 2500.3547

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
722.3817	722.3473	47.6023	239	244	(R)(S)
1284.8097	1284.7527	44.3549	168	178	(R)(T)
1475.8201	1475.7780	28.5656	141	152	(K)MELQEIQLKEAK(H)	1Met-ox
1727.9426	1727.8928	28.8025	92	105	(R)(L)
1799.9990	1799.9867	6.8457	168	182	(R)(A)
1815.9606	1815.8990	33.9709	153	167	(K)(K)
1884.0871	1883.9939	49.4569	91	105	(R)(L)
2202.2209	2202.1254	43.3943	106	125	(R)(G)
2556.4813	2556.3707	43.2646	129	149	(K)(E)

The matched peptides cover 33% (95/284 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/22 matches (31%). 77094.8 Da, pI = 6.53. Acc. # P05165. HUMAN. PROPIONYL-COA CARBOXYLASE ALPHA CHAIN PRECURSOR (EC 6.4.1.3) (PCCASE) (PROPANOYL-COA:CARBON DIOXIDE LIGASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.8201 1475.6874 89.9148 80 94 (K)(S)
1770.9487 1770.8784 39.7385 11 24 (K)HVLYYSRQCLMVSR(N) 1Met-ox
1770.9487 1770.8948 30.4653 623 637 (K)FMLEKVTEDTSSVLR(S) 1Met-ox
1884.0871 1883.9723 60.9218 95 110 (K)(A)
1999.0801 1999.1592 -39.5646 157 175 (K)(D)
2202.2209 2202.1519 31.3649 270 288 (R)(R)
2202.2209 2202.1519 31.3649 271 289 (R)(A)
2211.2081 2211.1021 47.9274 329 348 (R)(T)
2556.4813 2556.3422 54.4228 162 185 (K)(E)
2556.4813 2556.2808 78.4256 471 493 (K)(S)

15 unmatched masses: 647.2992 656.0685 716.2445 722.3817 842.5409 1284.8097 1638.9129 1699.9109 1727.9426 1799.9990 1815.9606 1962.0786 2127.1517 2384.0718 2500.3547

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.8201	1475.6874	89.9148	80	94	(K)(S)
1770.9487	1770.8784	39.7385	11	24	(K)HVLYYSRQCLMVSR(N)	1Met-ox
1770.9487	1770.8948	30.4653	623	637	(K)FMLEKVTEDTSSVLR(S)	1Met-ox
1884.0871	1883.9723	60.9218	95	110	(K)(A)
1999.0801	1999.1592	-39.5646	157	175	(K)(D)
2202.2209	2202.1519	31.3649	270	288	(R)(R)
2202.2209	2202.1519	31.3649	271	289	(R)(A)
2211.2081	2211.1021	47.9274	329	348	(R)(T)
2556.4813	2556.3422	54.4228	162	185	(K)(E)
2556.4813	2556.2808	78.4256	471	493	(K)(S)

The matched peptides cover 21% (152/702 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/22 matches (22%). 51862.3 Da, pI = 6.15. Acc. # P00439. HUMAN. PHENYLALANINE-4-HYDROXYLASE (EC 1.14.16.1) (PAH) (PHE-4- MONOOXYGENASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
647.2992 647.3364 -57.4486 336 341 (K)(A)
1475.8201 1475.8123 5.2883 401 413 (R)(Y)
1727.9426 1727.8393 59.7624 74 86 (K)(S)
1799.9990 1800.0424 -24.0784 185 199 (K)(T)
2500.3547 2500.2248 51.9901 298 320 (R)(L)

17 unmatched masses: 656.0685 716.2445 722.3817 842.5409 1284.8097 1638.9129 1699.9109 1770.9487 1815.9606 1884.0871 1962.0786 1999.0801 2127.1517 2202.2209 2211.2081 2384.0718 2556.4813

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
647.2992	647.3364	-57.4486	336	341	(K)(A)
1475.8201	1475.8123	5.2883	401	413	(R)(Y)
1727.9426	1727.8393	59.7624	74	86	(K)(S)
1799.9990	1800.0424	-24.0784	185	199	(K)(T)
2500.3547	2500.2248	51.9901	298	320	(R)(L)

The matched peptides cover 15% (70/452 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):