MS-Fit Search Results

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Sample ID (comment): HSG031
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 665 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
29

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	5.74e+003	11/29 (37%)	44596.9 / 8.30	HUMAN	PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) (PRIMER RECOGNITION PROTEIN 2) (PRP 2).
2	2.44e+003	9/29 (31%)	44405.5 / 7.52	MOUSE	PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3).
3	648	9/29 (31%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
4	583	8/29 (27%)	83674.3 / 6.73	HUMAN	PROTEIN KINASE C, EPSILON TYPE (EC 2.7.1.-) (NPKC-EPSILON).
5	500	8/29 (27%)	80547.2 / 4.67	HUMAN	NIL-2-A ZINC FINGER PROTEIN (NEGATIVE REGULATOR OF IL2) (TRANSCRIPTION FACTOR 8).

Detailed Results

1. 11/29 matches (37%). 44596.9 Da, pI = 8.30. Acc. # P00558. HUMAN. PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) (PRIMER RECOGNITION PROTEIN 2) (PRP 2)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
809.3790	809.4157	-45.3618	323	329	(K)(A)
1634.7931	1634.7927	0.2769	156	170	(K)(A)
1768.9795	1768.9961	-9.4326	199	215	(K)(V)
1782.9264	1782.9536	-15.2502	106	122	(K)ACANPAAGSVILLENLR(F)	1Cys-am
1982.9467	1982.9381	4.3609	246	263	(K)VLNNMEIGTSLFDEEGAK(I)	1Met-ox
2023.0301	2023.0388	-4.2757	279	296	(K)(T)
2044.9877	2045.1283	-68.7501	272	290	(K)(F)
2048.0493	2047.9983	24.8863	22	38	(R)VDFNVPMKNNQITNNQR(I)	1Met-ox
2105.0648	2105.0609	1.8761	332	349	(K)(G)
2121.0475	2121.0729	-11.9539	151	170	(R)(A)
2705.1672	2705.3728	-76.0025	17	38	(K)RVVMRVDFNVPMKNNQITNNQR(I)	2Met-ox

18 unmatched masses: 656.0824 666.0106 842.5185 1475.7657 1487.7411 1638.8533 1648.7836 1891.0217 1941.9050 1993.9570 2150.0758 2383.9436 2465.2201 2466.1464 2717.1255 2718.0654 2902.4324 3264.5378

The matched peptides cover 34% (144/417 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/29 matches (31%). 44405.5 Da, pI = 7.52. Acc. # P09411. MOUSE. PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1634.7931 1634.7927 0.2769 156 170 (K)(A)
1768.9795 1768.9961 -9.4326 199 215 (K)(V)
1982.9467 1982.9381 4.3609 246 263 (K)(I)
2023.0301 2023.0388 -4.2757 279 296 (K)(T)
2044.9877 2045.1283 -68.7501 272 290 (K)(F)
2048.0493 2047.9983 24.8863 22 38 (R)VDFNVPMKNNQITNNQR(I) 1Met-ox
2105.0648 2105.0609 1.8761 332 349 (K)(G)
2121.0475 2121.0729 -11.9539 151 170 (R)(A)
2705.1672 2705.3728 -76.0025 17 38 (K)RVVMRVDFNVPMKNNQITNNQR(I) 2Met-ox

20 unmatched masses: 656.0824 666.0106 809.3790 842.5185 1475.7657 1487.7411 1638.8533 1648.7836 1782.9264 1891.0217 1941.9050 1993.9570 2150.0758 2383.9436 2465.2201 2466.1464 2717.1255 2718.0654 2902.4324 3264.5378

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1634.7931	1634.7927	0.2769	156	170	(K)(A)
1768.9795	1768.9961	-9.4326	199	215	(K)(V)
1982.9467	1982.9381	4.3609	246	263	(K)(I)
2023.0301	2023.0388	-4.2757	279	296	(K)(T)
2044.9877	2045.1283	-68.7501	272	290	(K)(F)
2048.0493	2047.9983	24.8863	22	38	(R)VDFNVPMKNNQITNNQR(I)	1Met-ox
2105.0648	2105.0609	1.8761	332	349	(K)(G)
2121.0475	2121.0729	-11.9539	151	170	(R)(A)
2705.1672	2705.3728	-76.0025	17	38	(K)RVVMRVDFNVPMKNNQITNNQR(I)	2Met-ox

The matched peptides cover 28% (120/416 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/29 matches (31%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7657 1475.7858 -13.6212 199 210 (R)(W)
1475.7657 1475.7494 11.0341 211 222 (K)(T)
1487.7411 1487.8043 -42.4545 239 250 (R)(L)
1638.8533 1638.8604 -4.3072 185 198 (K)(F)
1941.9050 1941.9882 -42.8058 443 459 (K)(L)
1993.9570 1993.9772 -10.1208 223 238 (R)(R)
2121.0475 2121.0114 17.0591 8 28 (R)(R)
2150.0758 2150.0783 -1.1766 223 239 (R)(R)
2383.9436 2383.9524 -3.7000 518 548 (R)(G)
2466.1464 2466.0553 36.9172 257 275 (K)NMQDMVEDYRNKYEDEINK(R) 2Met-ox

20 unmatched masses: 656.0824 666.0106 809.3790 842.5185 1634.7931 1648.7836 1768.9795 1782.9264 1891.0217 1982.9467 2023.0301 2044.9877 2048.0493 2105.0648 2465.2201 2705.1672 2717.1255 2718.0654 2902.4324 3264.5378

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7657	1475.7858	-13.6212	199	210	(R)(W)
1475.7657	1475.7494	11.0341	211	222	(K)(T)
1487.7411	1487.8043	-42.4545	239	250	(R)(L)
1638.8533	1638.8604	-4.3072	185	198	(K)(F)
1941.9050	1941.9882	-42.8058	443	459	(K)(L)
1993.9570	1993.9772	-10.1208	223	238	(R)(R)
2121.0475	2121.0114	17.0591	8	28	(R)(R)
2150.0758	2150.0783	-1.1766	223	239	(R)(R)
2383.9436	2383.9524	-3.7000	518	548	(R)(G)
2466.1464	2466.0553	36.9172	257	275	(K)NMQDMVEDYRNKYEDEINK(R)	2Met-ox

The matched peptides cover 23% (154/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/29 matches (27%). 83674.3 Da, pI = 6.73. Acc. # Q02156. HUMAN. PROTEIN KINASE C, EPSILON TYPE (EC 2.7.1.-) (NPKC-EPSILON)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5185 842.5073 13.2593 156 162 (K)(R)
842.5185 842.5073 13.2593 157 163 (R)(V)
1475.7657 1475.7164 33.4063 650 663 (R)LGCVASQNGEDAIK(Q) 1Cys-am
1634.7931 1634.8641 -43.4171 125 140 (R)(D)
1941.9050 1942.0050 -51.4614 269 283 (R)QGLQCKVCKMNVHRR(C) 2Cys-am
2044.9877 2045.0021 -7.0284 283 301 (R)RCETNVAPNCGVDARGIAK(V) 1Cys-am
2048.0493 2047.8932 76.2251 60 77 (K)(K)
2048.0493 2047.9305 58.0327 172 187 (K)FMATYLRQPTYCSHCR(D) 1Cys-am
2383.9436 2384.1134 -71.2362 278 297 (K)MNVHRRCETNVAPNCGVDAR(G) 2Cys-am
2902.4324 2902.4312 0.4093 197 219 (K)QGYQCQVCTCVVHKRCHELIITK(C) 3Cys-am

21 unmatched masses: 656.0824 666.0106 809.3790 1487.7411 1638.8533 1648.7836 1768.9795 1782.9264 1891.0217 1982.9467 1993.9570 2023.0301 2105.0648 2121.0475 2150.0758 2465.2201 2466.1464 2705.1672 2717.1255 2718.0654 3264.5378

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5185	842.5073	13.2593	156	162	(K)(R)
842.5185	842.5073	13.2593	157	163	(R)(V)
1475.7657	1475.7164	33.4063	650	663	(R)LGCVASQNGEDAIK(Q)	1Cys-am
1634.7931	1634.8641	-43.4171	125	140	(R)(D)
1941.9050	1942.0050	-51.4614	269	283	(R)QGLQCKVCKMNVHRR(C)	2Cys-am
2044.9877	2045.0021	-7.0284	283	301	(R)RCETNVAPNCGVDARGIAK(V)	1Cys-am
2048.0493	2047.8932	76.2251	60	77	(K)(K)
2048.0493	2047.9305	58.0327	172	187	(K)FMATYLRQPTYCSHCR(D)	1Cys-am
2383.9436	2384.1134	-71.2362	278	297	(K)MNVHRRCETNVAPNCGVDAR(G)	2Cys-am
2902.4324	2902.4312	0.4093	197	219	(K)QGYQCQVCTCVVHKRCHELIITK(C)	3Cys-am

The matched peptides cover 17% (128/737 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/29 matches (27%). 80547.2 Da, pI = 4.67. Acc. # P37275. HUMAN. NIL-2-A ZINC FINGER PROTEIN (NEGATIVE REGULATOR OF IL2) (TRANSCRIPTION FACTOR 8)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7657 1475.6953 47.7250 564 575 (R)LHSGEKPYQCDK(C) 1Cys-am
2023.0301 2022.9754 27.0417 533 549 (R)(K)
2121.0475 2120.9455 48.1054 510 526 (K)TENGMYACDLCDKIFQK(S) 2Cys-am
2465.2201 2465.1450 30.4984 507 526 (K)MRKTENGMYACDLCDKIFQK(S) 1Cys-am
2705.1672 2705.2515 -31.1826 269 293 (K)(M)
2717.1255 2717.3668 -88.7652 106 129 (K)ENPVATNSCKSEKLPEDLTVKSEK(D) 1Cys-am
2718.0654 2718.2166 -55.6430 579 599 (K)RFSHSGSYSQHMNHRYSYCKR(E) 1Met-ox 1Cys-am
2902.4324 2902.4139 6.3663 556 579 (K)(F)

21 unmatched masses: 656.0824 666.0106 809.3790 842.5185 1487.7411 1634.7931 1638.8533 1648.7836 1768.9795 1782.9264 1891.0217 1941.9050 1982.9467 1993.9570 2044.9877 2048.0493 2105.0648 2150.0758 2383.9436 2466.1464 3264.5378

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7657	1475.6953	47.7250	564	575	(R)LHSGEKPYQCDK(C)	1Cys-am
2023.0301	2022.9754	27.0417	533	549	(R)(K)
2121.0475	2120.9455	48.1054	510	526	(K)TENGMYACDLCDKIFQK(S)	2Cys-am
2465.2201	2465.1450	30.4984	507	526	(K)MRKTENGMYACDLCDKIFQK(S)	1Cys-am
2705.1672	2705.2515	-31.1826	269	293	(K)(M)
2717.1255	2717.3668	-88.7652	106	129	(K)ENPVATNSCKSEKLPEDLTVKSEK(D)	1Cys-am
2718.0654	2718.2166	-55.6430	579	599	(K)RFSHSGSYSQHMNHRYSYCKR(E)	1Met-ox 1Cys-am
2902.4324	2902.4139	6.3663	556	579	(K)(F)

The matched peptides cover 17% (130/735 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):