MS-Fit Search Results

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Sample ID (comment): HSG032
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 54 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
24

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	652	6/24 (25%)	16701.2 / 5.08	HUMAN	INITIATION FACTOR 5A (EIF-5A) (EIF-4D) (REV BINDING FACTOR).
2	296	7/24 (29%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	40.8	4/24 (16%)	53543.3 / 5.52	HUMAN	KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8).
3	40.6	4/24 (16%)	30498.3 / 8.67	MOUSE	T-CELL SURFACE GLYCOPROTEIN YE1/48 (T LYMPHOCYTE ANTIGEN A1) (LY49-A ANTIGEN).
4	30.7	4/24 (16%)	79102.9 / 6.56	MOUSE	MYB-RELATED PROTEIN B (B-MYB).

Detailed Results

1. 6/24 matches (25%). 16701.2 Da, pI = 5.08. Acc. # P10159. HUMAN. INITIATION FACTOR 5A (EIF-5A) (EIF-4D) (REV BINDING FACTOR)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1298.7306	1298.7472	-12.8529	55	66	(K)(K)
1341.6909	1341.7014	-7.8718	109	120	(R)(E)
1426.7962	1426.8422	-32.2731	55	67	(K)(Y)
1968.9812	1969.0242	-21.8482	109	125	(R)(Y)
2580.2372	2580.2946	-22.2335	86	108	(R)(E)
2736.3061	2736.3957	-32.7463	85	108	(K)(E)

18 unmatched masses: 656.0755 842.5132 1036.5219 1045.5687 1179.5934 1277.6917 1475.7501 1487.7362 1716.8214 1766.7351 1791.6653 1794.7496 2299.1347 2383.8771 2613.0703 2657.0690 2702.0831 2718.0782

The matched peptides cover 35% (54/153 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/24 matches (29%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5687 1045.5754 -6.4225 177 184 (K)(S)
1179.5934 1179.6010 -6.4092 376 385 (K)(H)
1277.6917 1277.6337 45.3784 246 256 (K)(N)
1277.6917 1277.7105 -14.7269 472 482 (K)(T)
1475.7501 1475.7858 -24.2169 199 210 (R)(W)
1475.7501 1475.7494 0.4381 211 222 (K)(T)
1487.7362 1487.8043 -45.7560 239 250 (R)(L)
1716.8214 1716.8517 -17.6345 417 431 (K)(G)
2383.8771 2383.9524 -31.5676 518 548 (R)(G)

17 unmatched masses: 656.0755 842.5132 1036.5219 1298.7306 1341.6909 1426.7962 1766.7351 1791.6653 1794.7496 1968.9812 2299.1347 2580.2372 2613.0703 2657.0690 2702.0831 2718.0782 2736.3061

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5687	1045.5754	-6.4225	177	184	(K)(S)
1179.5934	1179.6010	-6.4092	376	385	(K)(H)
1277.6917	1277.6337	45.3784	246	256	(K)(N)
1277.6917	1277.7105	-14.7269	472	482	(K)(T)
1475.7501	1475.7858	-24.2169	199	210	(R)(W)
1475.7501	1475.7494	0.4381	211	222	(K)(T)
1487.7362	1487.8043	-45.7560	239	250	(R)(L)
1716.8214	1716.8517	-17.6345	417	431	(K)(G)
2383.8771	2383.9524	-31.5676	518	548	(R)(G)

The matched peptides cover 18% (117/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/24 matches (16%). 53543.3 Da, pI = 5.52. Acc. # P05787. HUMAN. KERATIN, TYPE II CYTOSKELETAL 8 (CYTOKERATIN 8) (K8) (CK 8)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5687 1045.5530 15.0653 149 157 (R)(L)
1277.6917 1277.7105 -14.7269 381 391 (K)(K)
1341.6909 1341.7490 -43.3617 316 327 (R)(A)
1475.7501 1475.6987 34.8443 401 413 (R)(T)

20 unmatched masses: 656.0755 842.5132 1036.5219 1179.5934 1298.7306 1426.7962 1487.7362 1716.8214 1766.7351 1791.6653 1794.7496 1968.9812 2299.1347 2383.8771 2580.2372 2613.0703 2657.0690 2702.0831 2718.0782 2736.3061

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5687	1045.5530	15.0653	149	157	(R)(L)
1277.6917	1277.7105	-14.7269	381	391	(K)(K)
1341.6909	1341.7490	-43.3617	316	327	(R)(A)
1475.7501	1475.6987	34.8443	401	413	(R)(T)

The matched peptides cover 9% (45/482 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/24 matches (16%). 30498.3 Da, pI = 8.67. Acc. # P20937. MOUSE. T-CELL SURFACE GLYCOPROTEIN YE1/48 (T LYMPHOCYTE ANTIGEN A1) (LY49-A ANTIGEN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1968.9812 1968.9094 36.4637 240 256 (R)LDNGNCDQVFICICGKR(L) 1Cys-am
2702.0831 2702.2068 -45.7789 138 158 (R)(T)
2718.0782 2718.2017 -45.4435 138 158 (R)GDKVYWFCYGMKCYYFVMDRK(T) 1Met-ox
2736.3061 2736.3739 -24.7867 103 124 (K)NKSIECDLLESLNRDQNRLYNK(T) 1Cys-am

20 unmatched masses: 656.0755 842.5132 1036.5219 1045.5687 1179.5934 1277.6917 1298.7306 1341.6909 1426.7962 1475.7501 1487.7362 1716.8214 1766.7351 1791.6653 1794.7496 2299.1347 2383.8771 2580.2372 2613.0703 2657.0690

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1968.9812	1968.9094	36.4637	240	256	(R)LDNGNCDQVFICICGKR(L)	1Cys-am
2702.0831	2702.2068	-45.7789	138	158	(R)(T)
2718.0782	2718.2017	-45.4435	138	158	(R)GDKVYWFCYGMKCYYFVMDRK(T)	1Met-ox
2736.3061	2736.3739	-24.7867	103	124	(K)NKSIECDLLESLNRDQNRLYNK(T)	1Cys-am

The matched peptides cover 22% (60/262 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/24 matches (16%). 79102.9 Da, pI = 6.56. Acc. # P48972. MOUSE. MYB-RELATED PROTEIN B (B-MYB)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1036.5219 1036.4852 35.3800 48 55 (R)(F)
1475.7501 1475.7759 -17.5115 44 55 (R)(F)
2580.2372 2580.2867 -19.1864 421 445 (R)(S)
2580.2372 2580.3390 -39.4651 669 691 (K)(L)
2736.3061 2736.3878 -29.8731 420 445 (R)(S)

20 unmatched masses: 656.0755 842.5132 1045.5687 1179.5934 1277.6917 1298.7306 1341.6909 1426.7962 1487.7362 1716.8214 1766.7351 1791.6653 1794.7496 1968.9812 2299.1347 2383.8771 2613.0703 2657.0690 2702.0831 2718.0782

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1036.5219	1036.4852	35.3800	48	55	(R)(F)
1475.7501	1475.7759	-17.5115	44	55	(R)(F)
2580.2372	2580.2867	-19.1864	421	445	(R)(S)
2580.2372	2580.3390	-39.4651	669	691	(K)(L)
2736.3061	2736.3878	-29.8731	420	445	(R)(S)

The matched peptides cover 8% (61/704 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):