MS-Fit Search Results

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Sample ID (comment): HSG033
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 79 entries (results displayed for top 10 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
150.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
23

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	43.2	4/23 (17%)	37793.4 / 8.36	MOUSE	CONNECTIVE TISSUE GROWTH FACTOR PRECURSOR (CTGF) (FISP-12 PROTEIN).
2	35.9	4/23 (17%)	64970.2 / 5.75	HUMAN	CYTOSOLIC PURINE 5'-NUCLEOTIDASE (EC 3.1.3.5).
3	35.3	5/23 (21%)	95202.5 / 8.94	MOUSE	GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 1 PRECURSOR (GLUTAMATE RECEPTOR 5) (GLUR-5).
3	35	4/23 (17%)	10800.5 / 8.91	HUMAN	10 KD HEAT SHOCK PROTEIN, MITOCHONDRIAL (HSP10) (10 KD CHAPERONIN) (CPN10).
3	34.9	4/23 (17%)	10831.6 / 8.17	MOUSE	10 KD HEAT SHOCK PROTEIN, MITOCHONDRIAL (HSP10) (10 KD CHAPERONIN) (CPN10).
4	29.8	4/23 (17%)	36166.3 / 7.06	HUMAN	L-LACTATE DEHYDROGENASE X CHAIN (EC 1.1.1.27) (LDH-C).
5	23.7	4/23 (17%)	46193.0 / 7.70	HUMAN	PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE TYPE II (EC 2.7.1.68) (1- PHOSPHATIDYLINOSITOL-4-PHOSPHATE KINASE) (PIP5KII) (PTDINS(4)P-5- KINASE B ISOFORM) (DIPHOSPHOINOSITIDE KINASE).
6	22.6	4/23 (17%)	95910.5 / 4.75	MOUSE	NEUROFILAMENT TRIPLET M PROTEIN (160 KD NEUROFILAMENT PROTEIN) (NF-M).
7	18	4/23 (17%)	55769.1 / 5.98	HUMAN	RAC-BETA SERINE/THREONINE KINASE (EC 2.7.1.-) (RAC-PK-BETA) (AKT2 KINASE).
8	16.3	4/23 (17%)	50771.6 / 8.21	HUMAN	RETINOIC ACID RECEPTOR ALPHA-1 (RAR-ALPHA-1).

Detailed Results

1. 4/23 matches (17%). 37793.4 Da, pI = 8.36. Acc. # P29268. MOUSE. CONNECTIVE TISSUE GROWTH FACTOR PRECURSOR (CTGF) (FISP-12 PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
691.2817	691.3813	-144.0324	91	97	(K)(D)
1035.5265	1035.5726	-44.5302	297	305	(R)(C)
2233.0239	2232.9584	29.3383	121	141	(K)(L)
2705.1480	2705.2156	-24.9636	196	219	(R)ANCLVQTTEWSACSKTCGMGISTR(V)	1Met-ox 2Cys-am
2705.1480	2705.3537	-76.0271	232	253	(K)QSRLCMVRPCEADLEENIKKGK(K)	1Met-ox 2Cys-am

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 692.2267 693.1498 734.1813 825.0558 842.4530 907.4630 1475.7016 1529.7409 1940.8740 1993.9189 2211.0619 2300.1003

The matched peptides cover 23% (83/348 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/23 matches (17%). 64970.2 Da, pI = 5.75. Acc. # P49902. HUMAN. CYTOSOLIC PURINE 5'-NUCLEOTIDASE (EC 3.1.3.5)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
691.2817 691.3813 -144.0324 239 244 (R)(V)
1035.5265 1035.4457 78.0349 178 186 (R)(D)
1940.8740 1940.9426 -35.3659 405 421 (K)(R)
1993.9189 1993.8103 54.4642 426 442 (K)(S)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 692.2267 693.1498 734.1813 825.0558 842.4530 907.4630 1475.7016 1529.7409 2211.0619 2233.0239 2300.1003 2705.1480

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
691.2817	691.3813	-144.0324	239	244	(R)(V)
1035.5265	1035.4457	78.0349	178	186	(R)(D)
1940.8740	1940.9426	-35.3659	405	421	(K)(R)
1993.9189	1993.8103	54.4642	426	442	(K)(S)

The matched peptides cover 8% (49/561 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/23 matches (21%). 95202.5 Da, pI = 8.94. Acc. # Q60934. MOUSE. GLUTAMATE RECEPTOR, IONOTROPIC KAINATE 1 PRECURSOR (GLUTAMATE RECEPTOR 5) (GLUR-5)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
692.2267 692.3103 -120.6850 397 402 (K)(I)
907.4630 907.5001 -40.9597 60 67 (K)(N)
1475.7016 1475.7203 -12.6715 420 432 (R)(T)
1940.8740 1941.0407 -85.9071 433 448 (R)(K)
2300.1003 2300.1273 -11.7174 222 241 (K)(Q)

18 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 693.1498 734.1813 825.0558 842.4530 1035.5265 1529.7409 1993.9189 2211.0619 2233.0239 2705.1480

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
692.2267	692.3103	-120.6850	397	402	(K)(I)
907.4630	907.5001	-40.9597	60	67	(K)(N)
1475.7016	1475.7203	-12.6715	420	432	(R)(T)
1940.8740	1941.0407	-85.9071	433	448	(R)(K)
2300.1003	2300.1273	-11.7174	222	241	(K)(Q)

The matched peptides cover 7% (63/836 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/23 matches (17%). 10800.5 Da, pI = 8.91. Acc. # Q04984. HUMAN. 10 KD HEAT SHOCK PROTEIN, MITOCHONDRIAL (HSP10) (10 KD CHAPERONIN) (CPN10)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
907.4630 907.5042 -45.3923 8 14 (K)(V)
1035.5265 1035.5991 -70.1079 7 14 (R)(V)
1475.7016 1475.8110 -74.1370 66 79 (K)(V)
1529.7409 1529.8004 -38.8733 80 91 (K)(D)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 692.2267 693.1498 734.1813 825.0558 842.4530 1940.8740 1993.9189 2211.0619 2233.0239 2300.1003 2705.1480

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
907.4630	907.5042	-45.3923	8	14	(K)(V)
1035.5265	1035.5991	-70.1079	7	14	(R)(V)
1475.7016	1475.8110	-74.1370	66	79	(K)(V)
1529.7409	1529.8004	-38.8733	80	91	(K)(D)

The matched peptides cover 33% (34/101 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/23 matches (17%). 10831.6 Da, pI = 8.17. Acc. # Q64433. MOUSE. 10 KD HEAT SHOCK PROTEIN, MITOCHONDRIAL (HSP10) (10 KD CHAPERONIN) (CPN10)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
907.4630 907.5042 -45.3923 8 14 (K)(V)
1035.5265 1035.5991 -70.1079 7 14 (R)(V)
1475.7016 1475.8110 -74.1370 66 79 (K)(V)
1529.7409 1529.8004 -38.8733 80 91 (K)(D)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 692.2267 693.1498 734.1813 825.0558 842.4530 1940.8740 1993.9189 2211.0619 2233.0239 2300.1003 2705.1480

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
907.4630	907.5042	-45.3923	8	14	(K)(V)
1035.5265	1035.5991	-70.1079	7	14	(R)(V)
1475.7016	1475.8110	-74.1370	66	79	(K)(V)
1529.7409	1529.8004	-38.8733	80	91	(K)(D)

The matched peptides cover 33% (34/101 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/23 matches (17%). 36166.3 Da, pI = 7.06. Acc. # P07864. HUMAN. L-LACTATE DEHYDROGENASE X CHAIN (EC 1.1.1.27) (LDH-C)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
691.2817 691.3813 -144.0324 112 117 (R)NVAIMK(S) 1Met-ox
842.4530 842.5100 -67.6435 149 156 (K)(V)
1475.7016 1475.7932 -62.1046 284 296 (K)(N)
2705.1480 2705.4150 -98.6915 305 327 (K)(D)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 692.2267 693.1498 734.1813 825.0558 907.4630 1035.5265 1529.7409 1940.8740 1993.9189 2211.0619 2233.0239 2300.1003

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
691.2817	691.3813	-144.0324	112	117	(R)NVAIMK(S)	1Met-ox
842.4530	842.5100	-67.6435	149	156	(K)(V)
1475.7016	1475.7932	-62.1046	284	296	(K)(N)
2705.1480	2705.4150	-98.6915	305	327	(K)(D)

The matched peptides cover 15% (50/331 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/23 matches (17%). 46193.0 Da, pI = 7.70. Acc. # P48426. HUMAN. PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE TYPE II (EC 2.7.1.68) (1- PHOSPHATIDYLINOSITOL-4-PHOSPHATE KINASE) (PIP5KII) (PTDINS(4)P-5- KINASE B ISOFORM) (DIPHOSPHOINOSITIDE KINASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4530 842.3579 112.8700 343 349 (K)(K)
1940.8740 1940.9102 -18.6924 103 118 (R)(S)
1993.9189 1993.7834 67.9513 286 303 (R)(D)
2705.1480 2705.2791 -48.4455 82 102 (K)ENMPSHFKFKEYCPMVFRNLR(E) 2Met-ox

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 692.2267 693.1498 734.1813 825.0558 907.4630 1035.5265 1475.7016 1529.7409 2211.0619 2233.0239 2300.1003

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.4530	842.3579	112.8700	343	349	(K)(K)
1940.8740	1940.9102	-18.6924	103	118	(R)(S)
1993.9189	1993.7834	67.9513	286	303	(R)(D)
2705.1480	2705.2791	-48.4455	82	102	(K)ENMPSHFKFKEYCPMVFRNLR(E)	2Met-ox

The matched peptides cover 15% (62/405 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/23 matches (17%). 95910.5 Da, pI = 4.75. Acc. # P08553. MOUSE. NEUROFILAMENT TRIPLET M PROTEIN (160 KD NEUROFILAMENT PROTEIN) (NF-M)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
692.2267 692.3190 -133.2946 373 377 (K)(H)
1035.5265 1035.4958 29.6279 793 803 (K)(Y)
1940.8740 1941.0293 -80.0261 693 708 (K)(A)
1993.9189 1993.9640 -22.6233 466 484 (K)(E)
1993.9189 1994.0632 -72.4002 605 622 (K)SPMPKSPVEEVKPKPEAK(A) 1Met-ox

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 693.1498 734.1813 825.0558 842.4530 907.4630 1475.7016 1529.7409 2211.0619 2233.0239 2300.1003 2705.1480

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
692.2267	692.3190	-133.2946	373	377	(K)(H)
1035.5265	1035.4958	29.6279	793	803	(K)(Y)
1940.8740	1941.0293	-80.0261	693	708	(K)(A)
1993.9189	1993.9640	-22.6233	466	484	(K)(E)
1993.9189	1994.0632	-72.4002	605	622	(K)SPMPKSPVEEVKPKPEAK(A)	1Met-ox

The matched peptides cover 8% (69/848 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/23 matches (17%). 55769.1 Da, pI = 5.98. Acc. # P31751. HUMAN. RAC-BETA SERINE/THREONINE KINASE (EC 2.7.1.-) (RAC-PK-BETA) (AKT2 KINASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
691.2817 691.3125 -44.6007 177 181 (R)YYAMK(I) 1Met-ox
1940.8740 1941.1537 -144.1130 369 386 (R)(K)
2233.0239 2233.0574 -15.0165 225 243 (R)(E)
2705.1480 2705.3761 -84.3186 348 368 (R)(F)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 692.2267 693.1498 734.1813 825.0558 842.4530 907.4630 1035.5265 1475.7016 1529.7409 1993.9189 2211.0619 2300.1003

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
691.2817	691.3125	-44.6007	177	181	(R)YYAMK(I)	1Met-ox
1940.8740	1941.1537	-144.1130	369	386	(R)(K)
2233.0239	2233.0574	-15.0165	225	243	(R)(E)
2705.1480	2705.3761	-84.3186	348	368	(R)(F)

The matched peptides cover 13% (63/481 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/23 matches (17%). 50771.6 Da, pI = 8.21. Acc. # P10276. HUMAN. RETINOIC ACID RECEPTOR ALPHA-1 (RAR-ALPHA-1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1993.9189 1993.9120 3.4245 140 155 (R)CQYCRLQKCFEVGMSK(E) 1Cys-am
2211.0619 2210.9864 34.1608 95 114 (K)(S)
2300.1003 2300.3164 -93.9391 348 366 (R)(R)
2705.1480 2705.3595 -78.1855 167 190 (K)(V)

19 unmatched masses: 604.2029 614.1086 618.1511 646.1598 650.1058 656.0227 672.1145 691.2817 692.2267 693.1498 734.1813 825.0558 842.4530 907.4630 1035.5265 1475.7016 1529.7409 1940.8740 2233.0239

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1993.9189	1993.9120	3.4245	140	155	(R)CQYCRLQKCFEVGMSK(E)	1Cys-am
2211.0619	2210.9864	34.1608	95	114	(K)(S)
2300.1003	2300.3164	-93.9391	348	366	(R)(R)
2705.1480	2705.3595	-78.1855	167	190	(K)(V)

The matched peptides cover 17% (79/462 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):