MS-Fit Search Results

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Sample ID (comment): HSG034
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 245 entries (results displayed for top 10 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
29

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	335	7/29 (24%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
2	294	6/29 (20%)	89811.6 / 5.00	MOUSE	CGMP-INHIBITED 3',5'-CYCLIC PHOSPHODIESTERASE B (EC 3.1.4.17) (CYCLIC GMP INHIBITED PHOSPHODIESTERASE B) (CGI-PDE B) (CGIPDE1) (FRAGMENT).
3	177	4/29 (13%)	18447.7 / 6.49	HUMAN	MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 4 (FRAGMENT).
4	121	5/29 (17%)	34985.7 / 5.39	HUMAN	FOLLISTATIN-RELATED PROTEIN PRECURSOR.
5	120	4/29 (13%)	45855.0 / 8.46	HUMAN	N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.150) (N-ACETYLGLUCOSAMINYLTRANSFERASE) (I-BRANCHING ENZYME) (IGNT).
6	111	6/29 (20%)	42190.1 / 6.64	MOUSE	GLUTAMINE SYNTHETASE (EC 6.3.1.2) (GLUTAMATE--AMMONIA LIGASE).
7	101	4/29 (13%)	37374.9 / 5.85	HUMAN	FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).
8	82.7	4/29 (13%)	45095.0 / 7.98	MOUSE	ZINC FINGER PROTEIN MLZ-4 (ZINC FINGER PROTEIN 46).
9	78.6	4/29 (13%)	57447.5 / 5.97	MOUSE	T-COMPLEX PROTEIN 1, BETA SUBUNIT (TCP-1-BETA) (CCT-BETA).
10	77.4	4/29 (13%)	25570.6 / 8.05	MOUSE	GLUTATHIONE S-TRANSFERASE GT9.3 (EC 2.5.1.18) (CLASS-MU).

Detailed Results

1. 7/29 matches (24%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5671	1045.5754	-7.9451	177	184	(K)(S)
1475.7452	1475.7858	-27.5345	199	210	(R)(W)
1475.7452	1475.7494	-2.8796	211	222	(K)(T)
1751.9579	1751.8272	74.6073	602	623	(R)(S)
1985.9341	1985.8949	19.7414	251	266	(R)(N)
1993.9573	1993.9772	-9.9879	223	238	(R)(R)
2300.1346	2300.1985	-27.7799	343	363	(R)(S)
2383.9512	2383.9524	-0.4948	518	548	(R)(G)

22 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1750.9001 1766.7425 1794.7760 1811.7704 1827.7836 2211.0759 2233.0673 2283.0970 2299.1272 2705.1361

The matched peptides cover 21% (138/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/29 matches (20%). 89811.6 Da, pI = 5.00. Acc. # Q61409. MOUSE. CGMP-INHIBITED 3',5'-CYCLIC PHOSPHODIESTERASE B (EC 3.1.4.17) (CYCLIC GMP INHIBITED PHOSPHODIESTERASE B) (CGI-PDE B) (CGIPDE1) (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1750.9001 1750.8521 27.3637 753 766 (R)(I)
1766.7425 1766.8471 -59.2043 753 766 (R)IFCQLMHHLTENHK(I) 1Met-ox
1811.7704 1811.8791 -59.9662 364 377 (K)(M)
1827.7836 1827.8740 -49.4559 364 377 (K)MNNWNFQIFELVEK(M) 1Met-ox
1993.9573 1993.9853 -14.0396 752 766 (R)RIFCQLMHHLTENHK(I) 1Met-ox 1Cys-am
2233.0673 2233.0532 6.2878 133 151 (R)RSSGASSLLTNEHCSRWDR(S) 1Cys-am

23 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1751.9579 1794.7760 1985.9341 2211.0759 2283.0970 2299.1272 2300.1346 2383.9512 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1750.9001	1750.8521	27.3637	753	766	(R)(I)
1766.7425	1766.8471	-59.2043	753	766	(R)IFCQLMHHLTENHK(I)	1Met-ox
1811.7704	1811.8791	-59.9662	364	377	(K)(M)
1827.7836	1827.8740	-49.4559	364	377	(K)MNNWNFQIFELVEK(M)	1Met-ox
1993.9573	1993.9853	-14.0396	752	766	(R)RIFCQLMHHLTENHK(I)	1Met-ox 1Cys-am
2233.0673	2233.0532	6.2878	133	151	(R)RSSGASSLLTNEHCSRWDR(S)	1Cys-am

The matched peptides cover 6% (48/799 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/29 matches (13%). 18447.7 Da, pI = 6.49. Acc. # O15439. HUMAN. MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 4 (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1751.9579 1751.9220 20.5282 98 111 (R)(E)
1827.7836 1827.8183 -19.0034 12 28 (K)(Q)
1985.9341 1986.1309 -99.1210 118 135 (K)(X)
2300.1346 2300.2800 -63.2102 142 162 (R)(-)

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1750.9001 1766.7425 1794.7760 1811.7704 1993.9573 2211.0759 2233.0673 2283.0970 2299.1272 2383.9512 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1751.9579	1751.9220	20.5282	98	111	(R)(E)
1827.7836	1827.8183	-19.0034	12	28	(K)(Q)
1985.9341	1986.1309	-99.1210	118	135	(K)(X)
2300.1346	2300.2800	-63.2102	142	162	(R)(-)

The matched peptides cover 42% (69/162 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/29 matches (17%). 34985.7 Da, pI = 5.39. Acc. # Q12841. HUMAN. FOLLISTATIN-RELATED PROTEIN PRECURSOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1750.9001 1750.8030 55.4311 271 285 (K)(Y)
1751.9579 1751.8387 68.0900 103 118 (K)SVSPSASPVVCYQSNR(D) 1Cys-am
1766.7425 1766.7979 -31.3933 271 285 (K)NQKGAQTQTEEEMTR(Y) 1Met-ox
1827.7836 1827.8556 -39.3842 48 62 (K)GEPTCLCIEQCKPHK(R) 2Cys-am
2299.1272 2299.0997 11.9446 28 47 (K)SKICANVFCGAGRECAVTEK(G) 3Cys-am

24 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1794.7760 1811.7704 1985.9341 1993.9573 2211.0759 2233.0673 2283.0970 2300.1346 2383.9512 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1750.9001	1750.8030	55.4311	271	285	(K)(Y)
1751.9579	1751.8387	68.0900	103	118	(K)SVSPSASPVVCYQSNR(D)	1Cys-am
1766.7425	1766.7979	-31.3933	271	285	(K)NQKGAQTQTEEEMTR(Y)	1Met-ox
1827.7836	1827.8556	-39.3842	48	62	(K)GEPTCLCIEQCKPHK(R)	2Cys-am
2299.1272	2299.0997	11.9446	28	47	(K)SKICANVFCGAGRECAVTEK(G)	3Cys-am

The matched peptides cover 21% (66/308 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/29 matches (13%). 45855.0 Da, pI = 8.46. Acc. # Q06430. HUMAN. N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.150) (N-ACETYLGLUCOSAMINYLTRANSFERASE) (I-BRANCHING ENZYME) (IGNT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5671 1045.5464 19.7837 165 173 (R)(D)
1750.9001 1750.9016 -0.8716 74 88 (K)(E)
1993.9573 1993.9516 2.8613 114 129 (R)AIYMPQNIYCVHVDEK(A) 1Cys-am
2233.0673 2233.1439 -34.3335 366 383 (K)(E)

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1475.7452 1751.9579 1766.7425 1794.7760 1811.7704 1827.7836 1985.9341 2211.0759 2283.0970 2299.1272 2300.1346 2383.9512 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5671	1045.5464	19.7837	165	173	(R)(D)
1750.9001	1750.9016	-0.8716	74	88	(K)(E)
1993.9573	1993.9516	2.8613	114	129	(R)AIYMPQNIYCVHVDEK(A)	1Cys-am
2233.0673	2233.1439	-34.3335	366	383	(K)(E)

The matched peptides cover 14% (58/400 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/29 matches (20%). 42190.1 Da, pI = 6.64. Acc. # P15105. MOUSE. GLUTAMINE SYNTHETASE (EC 6.3.1.2) (GLUTAMATE--AMMONIA LIGASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7452 1475.7932 -32.5443 92 103 (R)DPNKLVLCEVFK(Y) 1Cys-am
1475.7452 1475.7892 -29.8185 269 280 (K)CIEEAIDKLSKR(H) 1Cys-am
1794.7760 1794.8849 -60.6647 26 41 (K)(C)
1993.9573 1994.0646 -53.8052 91 106 (R)(K)
2283.0970 2283.1378 -17.8878 26 45 (K)(T)
2299.1272 2299.1327 -2.4152 26 45 (K)VQAMYIWVDGTGEGLRCKTR(T) 1Met-ox
2705.1361 2705.3396 -75.2176 299 324 (R)(I)

23 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1750.9001 1751.9579 1766.7425 1811.7704 1827.7836 1985.9341 2211.0759 2233.0673 2300.1346 2383.9512

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1475.7452	1475.7932	-32.5443	92	103	(R)DPNKLVLCEVFK(Y)	1Cys-am
1475.7452	1475.7892	-29.8185	269	280	(K)CIEEAIDKLSKR(H)	1Cys-am
1794.7760	1794.8849	-60.6647	26	41	(K)(C)
1993.9573	1994.0646	-53.8052	91	106	(R)(K)
2283.0970	2283.1378	-17.8878	26	45	(K)(T)
2299.1272	2299.1327	-2.4152	26	45	(K)VQAMYIWVDGTGEGLRCKTR(T)	1Met-ox
2705.1361	2705.3396	-75.2176	299	324	(R)(I)

The matched peptides cover 19% (74/373 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/29 matches (13%). 37374.9 Da, pI = 5.85. Acc. # P19883. HUMAN. FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1475.7452 1475.7130 21.7766 152 163 (K)(C)
1811.7704 1811.8818 -61.4667 239 255 (K)(C)
2299.1272 2299.1765 -21.4564 6 25 (R)(S)
2705.1361 2705.3503 -79.1962 141 163 (R)(C)

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1750.9001 1751.9579 1766.7425 1794.7760 1827.7836 1985.9341 1993.9573 2211.0759 2233.0673 2283.0970 2300.1346 2383.9512

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1475.7452	1475.7130	21.7766	152	163	(K)(C)
1811.7704	1811.8818	-61.4667	239	255	(K)(C)
2299.1272	2299.1765	-21.4564	6	25	(R)(S)
2705.1361	2705.3503	-79.1962	141	163	(R)(C)

The matched peptides cover 17% (60/338 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/29 matches (13%). 45095.0 Da, pI = 7.98. Acc. # Q03309. MOUSE. ZINC FINGER PROTEIN MLZ-4 (ZINC FINGER PROTEIN 46)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1750.9001 1750.7893 63.2770 334 348 (R)THTGEKPYECVQCGK(G) 1Cys-am
1766.7425 1766.7842 -23.6191 278 292 (R)THTGEKPYECTACGK(S) 2Cys-am
2299.1272 2298.9984 56.0051 110 128 (R)THTGERPYDCNECGKSFGR(S) 2Cys-am
2705.1361 2705.3040 -62.0898 60 81 (R)YHICSQCGKAFSQISDLNRHQK(T) 2Cys-am

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1751.9579 1794.7760 1811.7704 1827.7836 1985.9341 1993.9573 2211.0759 2233.0673 2283.0970 2300.1346 2383.9512

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1750.9001	1750.7893	63.2770	334	348	(R)THTGEKPYECVQCGK(G)	1Cys-am
1766.7425	1766.7842	-23.6191	278	292	(R)THTGEKPYECTACGK(S)	2Cys-am
2299.1272	2298.9984	56.0051	110	128	(R)THTGERPYDCNECGKSFGR(S)	2Cys-am
2705.1361	2705.3040	-62.0898	60	81	(R)YHICSQCGKAFSQISDLNRHQK(T)	2Cys-am

The matched peptides cover 18% (71/393 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 4/29 matches (13%). 57447.5 Da, pI = 5.97. Acc. # P80314. MOUSE. T-COMPLEX PROTEIN 1, BETA SUBUNIT (TCP-1-BETA) (CCT-BETA)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
647.2590 647.3153 -86.9100 177 181 (K)(L)
1811.7704 1811.9002 -71.6270 155 170 (R)(L)
1827.7836 1827.8951 -61.0147 155 170 (R)FWQDLMNIAGTTLSSK(L) 1Met-ox
2383.9512 2384.1806 -96.2128 132 154 (R)(F)

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1750.9001 1751.9579 1766.7425 1794.7760 1985.9341 1993.9573 2211.0759 2233.0673 2283.0970 2299.1272 2300.1346 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
647.2590	647.3153	-86.9100	177	181	(K)(L)
1811.7704	1811.9002	-71.6270	155	170	(R)(L)
1827.7836	1827.8951	-61.0147	155	170	(R)FWQDLMNIAGTTLSSK(L)	1Met-ox
2383.9512	2384.1806	-96.2128	132	154	(R)(F)

The matched peptides cover 8% (44/535 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/29 matches (13%). 25570.6 Da, pI = 8.05. Acc. # P19639. MOUSE. GLUTATHIONE S-TRANSFERASE GT9.3 (EC 2.5.1.18) (CLASS-MU)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1751.9579 1751.8613 55.1602 168 181 (R)MFEPKCLDAFPNLR(D) 1Cys-am
1993.9573 1993.9911 -16.9682 152 167 (K)(M)
2283.0970 2283.1418 -19.6497 168 186 (R)(F)
2299.1272 2299.0930 14.8769 31 49 (K)(F)
2299.1272 2299.1368 -4.1649 168 186 (R)MFEPKCLDAFPNLRDFLAR(F) 1Met-ox

25 unmatched masses: 612.2146 614.1165 624.1824 631.2284 647.2590 650.1186 656.0709 666.0335 714.2292 723.3477 825.1092 842.5214 1045.5671 1475.7452 1750.9001 1766.7425 1794.7760 1811.7704 1827.7836 1985.9341 2211.0759 2233.0673 2300.1346 2383.9512 2705.1361

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1751.9579	1751.8613	55.1602	168	181	(R)MFEPKCLDAFPNLR(D)	1Cys-am
1993.9573	1993.9911	-16.9682	152	167	(K)(M)
2283.0970	2283.1418	-19.6497	168	186	(R)(F)
2299.1272	2299.0930	14.8769	31	49	(K)(F)
2299.1272	2299.1368	-4.1649	168	186	(R)MFEPKCLDAFPNLRDFLAR(F)	1Met-ox

The matched peptides cover 24% (54/217 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):