MS-Fit Search Results

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Sample ID (comment): HSG035
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 371 entries (results displayed for top 10 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
27

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.01e+003	8/27 (29%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
2	152	5/27 (18%)	58628.7 / 9.06	MOUSE	TBX6 PROTEIN (T-BOX PROTEIN 6).
3	146	4/27 (14%)	61987.6 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).
4	126	5/27 (18%)	31393.3 / 9.04	MOUSE	T-CELL SURFACE GLYCOPROTEIN LY-49H (LY49-H ANTIGEN).
5	97.2	4/27 (14%)	84791.5 / 6.69	HUMAN	CELL SURFACE ADHESION GLYCOPROTEINS LFA-1/CR3/P150,95 BETA-SUBUNIT PRECURSOR (INTEGRIN BETA-2) (CD18 ANTIGEN) (COMPLEMENT RECEPTOR C3 BETA-SUBUNIT).
6	82.8	5/27 (18%)	30723.4 / 8.62	MOUSE	INTERLEUKIN-2 RECEPTOR ALPHA CHAIN PRECURSOR (IL-2 RECEPTOR ALPHA SUBUNIT) (P55) (TAC ANTIGEN) (CD25).
7	78.5	4/27 (14%)	6068.4 / 8.38	HUMAN	METALLOTHIONEIN-IL (MT-1L) (MT1X).
7	78	5/27 (18%)	64613.4 / 6.28	HUMAN	MAGUK P55 SUBFAMILY MEMBER 2 (MPP2 PROTEIN) (DISCS, LARGE HOMOLOG 2).
8	74.9	4/27 (14%)	60697.4 / 4.99	MOUSE	INTEGRAL MEMBRANE PROTEIN DGCR2/IDD PRECURSOR (SEIZURE-RELATED MEMBRANE-BOUND ADHESION PROTEIN).
9	74.2	4/27 (14%)	59635.0 / 5.48	HUMAN	T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA) (KIAA0002).

Detailed Results

1. 8/27 matches (29%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5722	1179.6010	-24.4288	376	385	(K)(H)
1383.6530	1383.6909	-27.3254	185	196	(K)(V)
1475.7103	1475.7858	-51.1836	199	210	(R)(W)
1475.7103	1475.7494	-26.5292	211	222	(K)(T)
1482.6298	1482.7148	-57.3387	616	632	(R)(F)
1487.6819	1487.8043	-82.2723	239	250	(R)(L)
1993.8809	1993.9772	-48.2757	223	238	(R)(R)
2167.0012	2167.1148	-52.4102	179	196	(R)(V)
2383.8331	2383.9524	-50.0336	518	548	(R)(G)

19 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1194.5383 1307.6304 1379.6906 1455.6536 1470.6365 1794.7423 1837.8386 1890.8911 2064.0515 2211.0058 2284.0359 2510.0133 2705.0341

The matched peptides cover 19% (128/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/27 matches (18%). 58628.7 Da, pI = 9.06. Acc. # P70327. MOUSE. TBX6 PROTEIN (T-BOX PROTEIN 6)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1837.8386 1837.8808 -22.9459 172 187 (R)(Q)
2064.0515 2064.0561 -2.2434 262 279 (K)IAANPFAKGFRENGRNCK(R) 1Cys-am
2167.0012 2167.1082 -49.3993 197 215 (K)LTNSTLDPHGHLILHSMHK(Y) 1Met-ox
2211.0058 2211.0835 -35.1257 17 37 (R)(Y)
2284.0359 2284.1616 -55.0299 394 413 (R)GLCLSGSSICTILCPTLHPR(G) 3Cys-am

22 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1307.6304 1379.6906 1383.6530 1455.6536 1470.6365 1475.7103 1482.6298 1487.6819 1794.7423 1890.8911 1993.8809 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1837.8386	1837.8808	-22.9459	172	187	(R)(Q)
2064.0515	2064.0561	-2.2434	262	279	(K)IAANPFAKGFRENGRNCK(R)	1Cys-am
2167.0012	2167.1082	-49.3993	197	215	(K)LTNSTLDPHGHLILHSMHK(Y)	1Met-ox
2211.0058	2211.0835	-35.1257	17	37	(R)(Y)
2284.0359	2284.1616	-55.0299	394	413	(R)GLCLSGSSICTILCPTLHPR(G)	3Cys-am

The matched peptides cover 17% (94/540 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/27 matches (14%). 61987.6 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6304 1307.6782 -36.5279 240 249 (R)(Q)
1837.8386 1837.9660 -69.2838 374 389 (R)(K)
2510.0133 2510.1323 -47.4095 449 471 (K)(I)
2705.0341 2705.1617 -47.1536 63 94 (R)(G)

23 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1379.6906 1383.6530 1455.6536 1470.6365 1475.7103 1482.6298 1487.6819 1794.7423 1890.8911 1993.8809 2064.0515 2167.0012 2211.0058 2284.0359 2383.8331

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1307.6304	1307.6782	-36.5279	240	249	(R)(Q)
1837.8386	1837.9660	-69.2838	374	389	(R)(K)
2510.0133	2510.1323	-47.4095	449	471	(K)(I)
2705.0341	2705.1617	-47.1536	63	94	(R)(G)

The matched peptides cover 13% (81/622 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/27 matches (18%). 31393.3 Da, pI = 9.04. Acc. # Q60682. MOUSE. T-CELL SURFACE GLYCOPROTEIN LY-49H (LY49-H ANTIGEN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6304 1307.5868 33.3677 122 131 (R)(S)
1455.6536 1455.6612 -5.2273 1 12 (-)(F)
1487.6819 1487.6510 20.7150 1 12 (-)MSEQEVTFPTMR(F) 2Met-ox
1794.7423 1794.8485 -59.1491 1 15 (-)SEQEVTFPTMRFHK(S) 1Met-ox Acet N
1837.8386 1837.7447 51.1187 244 259 (R)(K)

22 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1379.6906 1383.6530 1470.6365 1475.7103 1482.6298 1890.8911 1993.8809 2064.0515 2167.0012 2211.0058 2284.0359 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6304	1307.5868	33.3677	122	131	(R)(S)
1455.6536	1455.6612	-5.2273	1	12	(-)(F)
1487.6819	1487.6510	20.7150	1	12	(-)MSEQEVTFPTMR(F)	2Met-ox
1794.7423	1794.8485	-59.1491	1	15	(-)SEQEVTFPTMRFHK(S)	1Met-ox Acet N
1837.8386	1837.7447	51.1187	244	259	(R)(K)

The matched peptides cover 15% (41/266 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/27 matches (14%). 84791.5 Da, pI = 6.69. Acc. # P05107. HUMAN. CELL SURFACE ADHESION GLYCOPROTEINS LFA-1/CR3/P150,95 BETA-SUBUNIT PRECURSOR (INTEGRIN BETA-2) (CD18 ANTIGEN) (COMPLEMENT RECEPTOR C3 BETA-SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1107.5181 1107.5006 15.8071 544 554 (R)(G)
1165.5670 1165.5464 17.6336 197 205 (K)ECQPPFAFR(H) 1Cys-am
1794.7423 1794.9060 -91.1906 415 429 (K)VTATECIQEQSFVIR(A) 1Cys-am
1993.8809 1993.8458 17.6273 528 543 (K)LIYGQYCECDTINCER(Y) 1Cys-am

23 unmatched masses: 609.2370 842.5001 1036.5096 1172.5049 1179.5722 1194.5383 1307.6304 1379.6906 1383.6530 1455.6536 1470.6365 1475.7103 1482.6298 1487.6819 1837.8386 1890.8911 2064.0515 2167.0012 2211.0058 2284.0359 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1107.5181	1107.5006	15.8071	544	554	(R)(G)
1165.5670	1165.5464	17.6336	197	205	(K)ECQPPFAFR(H)	1Cys-am
1794.7423	1794.9060	-91.1906	415	429	(K)VTATECIQEQSFVIR(A)	1Cys-am
1993.8809	1993.8458	17.6273	528	543	(K)LIYGQYCECDTINCER(Y)	1Cys-am

The matched peptides cover 6% (51/769 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/27 matches (18%). 30723.4 Da, pI = 8.62. Acc. # P01590. MOUSE. INTERLEUKIN-2 RECEPTOR ALPHA CHAIN PRECURSOR (IL-2 RECEPTOR ALPHA SUBUNIT) (P55) (TAC ANTIGEN) (CD25)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1165.5670 1165.5346 27.8310 43 52 (K)NGTILNCECK(R) 1Cys-am
1837.8386 1837.9278 -48.4996 43 57 (K)NGTILNCECKRGFRR(L) 1Cys-am
1890.8911 1891.0080 -61.8094 154 170 (K)ALQRGPAISICKMKCGK(T) 1Met-ox 1Cys-am
2064.0515 2063.9643 42.2664 168 184 (K)CGKTGWTQPQLTCVDER(E) 2Cys-am
2284.0359 2283.9181 51.5640 66 84 (R)CLGNSWSSNCQCTSNSHDK(S) 3Cys-am

22 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1172.5049 1179.5722 1194.5383 1307.6304 1379.6906 1383.6530 1455.6536 1470.6365 1475.7103 1482.6298 1487.6819 1794.7423 1993.8809 2167.0012 2211.0058 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1165.5670	1165.5346	27.8310	43	52	(K)NGTILNCECK(R)	1Cys-am
1837.8386	1837.9278	-48.4996	43	57	(K)NGTILNCECKRGFRR(L)	1Cys-am
1890.8911	1891.0080	-61.8094	154	170	(K)ALQRGPAISICKMKCGK(T)	1Met-ox 1Cys-am
2064.0515	2063.9643	42.2664	168	184	(K)CGKTGWTQPQLTCVDER(E)	2Cys-am
2284.0359	2283.9181	51.5640	66	84	(R)CLGNSWSSNCQCTSNSHDK(S)	3Cys-am

The matched peptides cover 24% (65/268 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/27 matches (14%). 6068.4 Da, pI = 8.38. Acc. # P80297. HUMAN. METALLOTHIONEIN-IL (MT-1L) (MT1X)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1455.6536 1455.6394 9.7360 44 56 (K)CAQGCICKGTSDK(C) 2Cys-am
1993.8809 1993.7732 54.0185 44 61 (K)CAQGCICKGTSDKCSCCA(-) 3Cys-am
2167.0012 2166.7879 98.4290 1 22 (-)MDPNCSCSPVGSCACAGSCKCK(E) 1Met-ox
2383.8331 2383.9492 -48.6813 23 43 (K)ECKCTSCKKSCCSCCPVGCAK(C) 3Cys-am

23 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1307.6304 1379.6906 1383.6530 1470.6365 1475.7103 1482.6298 1487.6819 1794.7423 1837.8386 1890.8911 2064.0515 2211.0058 2284.0359 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1455.6536	1455.6394	9.7360	44	56	(K)CAQGCICKGTSDK(C)	2Cys-am
1993.8809	1993.7732	54.0185	44	61	(K)CAQGCICKGTSDKCSCCA(-)	3Cys-am
2167.0012	2166.7879	98.4290	1	22	(-)MDPNCSCSPVGSCACAGSCKCK(E)	1Met-ox
2383.8331	2383.9492	-48.6813	23	43	(K)ECKCTSCKKSCCSCCPVGCAK(C)	3Cys-am

The matched peptides cover 98% (60/61 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/27 matches (18%). 64613.4 Da, pI = 6.28. Acc. # Q14168. HUMAN. MAGUK P55 SUBFAMILY MEMBER 2 (MPP2 PROTEIN) (DISCS, LARGE HOMOLOG 2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.5001 842.5100 -11.7807 223 229 (R)(N)
1194.5383 1194.5002 31.8641 256 264 (K)CHFDYDPAR(D) 1Cys-am
1455.6536 1455.7807 -87.3282 89 100 (R)(D)
2211.0058 2211.1886 -82.6703 170 190 (K)(I)
2284.0359 2284.2389 -88.8523 358 375 (R)(T)

22 unmatched masses: 609.2370 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1307.6304 1379.6906 1383.6530 1470.6365 1475.7103 1482.6298 1487.6819 1794.7423 1837.8386 1890.8911 1993.8809 2064.0515 2167.0012 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
842.5001	842.5100	-11.7807	223	229	(R)(N)
1194.5383	1194.5002	31.8641	256	264	(K)CHFDYDPAR(D)	1Cys-am
1455.6536	1455.7807	-87.3282	89	100	(R)(D)
2211.0058	2211.1886	-82.6703	170	190	(K)(I)
2284.0359	2284.2389	-88.8523	358	375	(R)(T)

The matched peptides cover 11% (67/576 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/27 matches (14%). 60697.4 Da, pI = 4.99. Acc. # P98154. MOUSE. INTEGRAL MEMBRANE PROTEIN DGCR2/IDD PRECURSOR (SEIZURE-RELATED MEMBRANE-BOUND ADHESION PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1794.7423 1794.7969 -30.3779 61 77 (K)(E)
2167.0012 2166.8968 48.1494 328 347 (K)(L)
2383.8331 2384.0256 -80.7240 299 319 (R)GEPEMCVAALCERPQGCQQYR(K) 1Met-ox
2510.0133 2510.1049 -36.4790 299 319 (R)GEPEMCVAALCERPQGCQQYR(K) 2Cys-am

23 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1307.6304 1379.6906 1383.6530 1455.6536 1470.6365 1475.7103 1482.6298 1487.6819 1837.8386 1890.8911 1993.8809 2064.0515 2211.0058 2284.0359 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1794.7423	1794.7969	-30.3779	61	77	(K)(E)
2167.0012	2166.8968	48.1494	328	347	(K)(L)
2383.8331	2384.0256	-80.7240	299	319	(R)GEPEMCVAALCERPQGCQQYR(K)	1Met-ox
2510.0133	2510.1049	-36.4790	299	319	(R)GEPEMCVAALCERPQGCQQYR(K)	2Cys-am

The matched peptides cover 10% (58/548 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 4/27 matches (14%). 59635.0 Da, pI = 5.48. Acc. # P50990. HUMAN. T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA) (KIAA0002)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6304 1307.6384 -6.1259 21 31 (K)(N)
1475.7103 1475.7640 -36.4250 32 44 (R)(T)
1837.8386 1837.9370 -53.5108 121 137 (R)(K)
1890.8911 1891.0475 -82.7171 521 539 (R)(K)

23 unmatched masses: 609.2370 842.5001 1036.5096 1107.5181 1165.5670 1172.5049 1179.5722 1194.5383 1379.6906 1383.6530 1455.6536 1470.6365 1482.6298 1487.6819 1794.7423 1993.8809 2064.0515 2167.0012 2211.0058 2284.0359 2383.8331 2510.0133 2705.0341

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1307.6304	1307.6384	-6.1259	21	31	(K)(N)
1475.7103	1475.7640	-36.4250	32	44	(R)(T)
1837.8386	1837.9370	-53.5108	121	137	(R)(K)
1890.8911	1891.0475	-82.7171	521	539	(R)(K)

The matched peptides cover 10% (60/548 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):