MS-Fit Search Results

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Sample ID (comment): HSG036
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 289 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.08e+003	7/20 (35%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
2	1.61e+003	6/20 (30%)	37374.9 / 5.85	HUMAN	FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).
3	265	5/20 (25%)	37866.4 / 5.42	MOUSE	FOLLISTATIN PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).
4	116	5/20 (25%)	61987.6 / 5.14	HUMAN	KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9).
5	106	8/20 (40%)	78242.3 / 8.97	HUMAN	ZINC FINGER PROTEIN 45 (BRC1744).

Detailed Results

1. 7/20 matches (35%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.5838	1179.6010	-14.5272	376	385	(K)(H)
1383.6592	1383.6909	-22.9047	185	196	(K)(V)
1475.7188	1475.7858	-45.4334	199	210	(R)(W)
1475.7188	1475.7494	-20.7789	211	222	(K)(T)
1487.7084	1487.8043	-64.4406	239	250	(R)(L)
1716.8033	1716.8517	-28.1922	417	431	(K)(G)
1993.9512	1993.9772	-13.0211	223	238	(R)(R)
2383.9057	2383.9524	-19.5971	518	548	(R)(G)

13 unmatched masses: 568.1150 656.0389 1235.5606 1312.5848 1323.6378 1493.6808 1707.7305 1791.6898 1940.9088 2211.0543 2299.1802 2705.1228 2717.1012

The matched peptides cover 18% (120/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/20 matches (30%). 37374.9 Da, pI = 5.85. Acc. # P19883. HUMAN. FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1312.5848 1312.5223 47.5700 267 278 (R)(S)
1383.6592 1383.5594 72.0705 267 278 (R)CSLCDELCPDSK(S) 1Cys-am
1475.7188 1475.7130 3.8768 152 163 (K)(C)
2299.1802 2299.1765 1.5936 6 25 (R)(S)
2705.1228 2705.3503 -84.1205 141 163 (R)(C)
2717.1012 2717.2809 -66.1331 39 60 (K)NGRCQVLYKTELSKEECCSTGR(L) 3Cys-am

14 unmatched masses: 568.1150 656.0389 1179.5838 1235.5606 1323.6378 1487.7084 1493.6808 1707.7305 1716.8033 1791.6898 1940.9088 1993.9512 2211.0543 2383.9057

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1312.5848	1312.5223	47.5700	267	278	(R)(S)
1383.6592	1383.5594	72.0705	267	278	(R)CSLCDELCPDSK(S)	1Cys-am
1475.7188	1475.7130	3.8768	152	163	(K)(C)
2299.1802	2299.1765	1.5936	6	25	(R)(S)
2705.1228	2705.3503	-84.1205	141	163	(R)(C)
2717.1012	2717.2809	-66.1331	39	60	(K)NGRCQVLYKTELSKEECCSTGR(L)	3Cys-am

The matched peptides cover 22% (77/338 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/20 matches (25%). 37866.4 Da, pI = 5.42. Acc. # P47931. MOUSE. FOLLISTATIN PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1312.5848 1312.5223 47.5700 267 278 (R)(S)
1383.6592 1383.5594 72.0705 267 278 (R)CSLCDELCPDSK(S) 1Cys-am
1475.7188 1475.7905 -48.6236 255 266 (K)KCLWDSKVGRGR(C) 1Cys-am
2299.1802 2299.1765 1.5936 6 25 (R)(S)
2717.1012 2717.2809 -66.1331 39 60 (K)NGRCQVLYKTELSKEECCSTGR(L) 3Cys-am

15 unmatched masses: 568.1150 656.0389 1179.5838 1235.5606 1323.6378 1487.7084 1493.6808 1707.7305 1716.8033 1791.6898 1940.9088 1993.9512 2211.0543 2383.9057 2705.1228

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1312.5848	1312.5223	47.5700	267	278	(R)(S)
1383.6592	1383.5594	72.0705	267	278	(R)CSLCDELCPDSK(S)	1Cys-am
1475.7188	1475.7905	-48.6236	255	266	(K)KCLWDSKVGRGR(C)	1Cys-am
2299.1802	2299.1765	1.5936	6	25	(R)(S)
2717.1012	2717.2809	-66.1331	39	60	(K)NGRCQVLYKTELSKEECCSTGR(L)	3Cys-am

The matched peptides cover 19% (66/344 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/20 matches (25%). 61987.6 Da, pI = 5.14. Acc. # P35527. HUMAN. KERATIN, TYPE I CYTOSKELETAL 9 (CYTOKERATIN 9) (K9) (CK 9)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1235.5606 1235.5293 25.3746 46 58 (R)(V)
1323.6378 1323.6731 -26.6934 240 249 (R)IKFEMEQNLR(Q) 1Met-ox
1791.6898 1791.7283 -21.4358 490 512 (R)(G)
2299.1802 2299.1202 26.1029 271 289 (K)(N)
2705.1228 2705.1617 -14.3822 63 94 (R)(G)

15 unmatched masses: 568.1150 656.0389 1179.5838 1312.5848 1383.6592 1475.7188 1487.7084 1493.6808 1707.7305 1716.8033 1940.9088 1993.9512 2211.0543 2383.9057 2717.1012

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1235.5606	1235.5293	25.3746	46	58	(R)(V)
1323.6378	1323.6731	-26.6934	240	249	(R)IKFEMEQNLR(Q)	1Met-ox
1791.6898	1791.7283	-21.4358	490	512	(R)(G)
2299.1802	2299.1202	26.1029	271	289	(K)(N)
2705.1228	2705.1617	-14.3822	63	94	(R)(G)

The matched peptides cover 15% (97/622 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/20 matches (40%). 78242.3 Da, pI = 8.97. Acc. # Q02386. HUMAN. ZINC FINGER PROTEIN 45 (BRC1744)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
568.1150 568.2223 -188.9503 474 479 (K)(G)
1312.5848 1312.5890 -3.2593 194 203 (K)CEKCDNAFRR(F) 1Cys-am
1383.6592 1383.6262 23.8497 194 203 (K)CEKCDNAFRR(F) 2Cys-am
1707.7305 1707.7835 -30.9879 437 451 (R)(G)
1707.7305 1707.7947 -37.5654 465 479 (R)GHTGEKPYKCGTCGK(G) 2Cys-am
1707.7305 1707.7835 -30.9879 633 647 (R)(G)
1716.8033 1716.7586 25.9968 306 318 (K)CEECGKSFSWRSR(L) 2Cys-am
1791.6898 1791.8158 -70.3004 521 535 (R)VHTGEKPYQCAECGK(G) 2Cys-am
2211.0543 2211.0504 1.7308 241 259 (R)VPTGENPYKYEECGRNVGK(S) 1Cys-am
2211.0543 2211.0552 -0.3986 502 520 (K)(V)
2705.1228 2705.3292 -76.3109 278 301 (K)(K)

12 unmatched masses: 656.0389 1179.5838 1235.5606 1323.6378 1475.7188 1487.7084 1493.6808 1940.9088 1993.9512 2299.1802 2383.9057 2717.1012

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
568.1150	568.2223	-188.9503	474	479	(K)(G)
1312.5848	1312.5890	-3.2593	194	203	(K)CEKCDNAFRR(F)	1Cys-am
1383.6592	1383.6262	23.8497	194	203	(K)CEKCDNAFRR(F)	2Cys-am
1707.7305	1707.7835	-30.9879	437	451	(R)(G)
1707.7305	1707.7947	-37.5654	465	479	(R)GHTGEKPYKCGTCGK(G)	2Cys-am
1707.7305	1707.7835	-30.9879	633	647	(R)(G)
1716.8033	1716.7586	25.9968	306	318	(K)CEECGKSFSWRSR(L)	2Cys-am
1791.6898	1791.8158	-70.3004	521	535	(R)VHTGEKPYQCAECGK(G)	2Cys-am
2211.0543	2211.0504	1.7308	241	259	(R)VPTGENPYKYEECGRNVGK(S)	1Cys-am
2211.0543	2211.0552	-0.3986	502	520	(K)(V)
2705.1228	2705.3292	-76.3109	278	301	(K)(K)

The matched peptides cover 21% (145/682 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):