MS-Fit Search Results

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Sample ID (comment): HSG038
Database searched: SwissProt.6.17.2000
Molecular weight search (1000 - 100000 Da) selects 82508 entries.
Full pI range: 87272 entries.
Species search ( HUMAN MOUSE ) selects 9626 entries.
Combined molecular weight, pI and species searches select 8686 entries.
MS-Fit search selects 33 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
13

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	114	5/13 (38%)	29819.3 / 8.30	HUMAN	FOLATE RECEPTOR ALPHA PRECURSOR (FR-ALPHA) (FOLATE RECEPTOR 1) (FOLATE RECEPTOR, ADULT) (ADULT FOLATE-BINDING PROTEIN) (FBP) (OVARIAN TUMOR-ASSOCIATED ANTIGEN MOV18) (KB CELLS FBP)
2	12.1	4/13 (30%)	66948.2 / 8.43	HUMAN	COMPLEMENT COMPONENT C8 BETA CHAIN PRECURSOR
3	9.58	4/13 (30%)	27381.8 / 9.56	MOUSE	PLATELET-DERIVED GROWTH FACTOR, B CHAIN PRECURSOR (PDGF B-CHAIN) (PDGF-2)
4	7.17	4/13 (30%)	58044.9 / 9.75	HUMAN	U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR 35 KD SUBUNIT RELATED-PROTEIN 2
4	7	6/13 (46%)	94300.5 / 5.18	HUMAN	HEAT SHOCK 70 KDA PROTEIN 4 (HEAT SHOCK 70-RELATED PROTEIN APG-2) (HSP70RY)

Detailed Results

1. 5/13 matches (38%). 29819.3 Da, pI = 8.30. Acc. # P15328. HUMAN. FOLATE RECEPTOR ALPHA PRECURSOR (FR-ALPHA) (FOLATE RECEPTOR 1) (FOLATE RECEPTOR, ADULT) (ADULT FOLATE-BINDING PROTEIN) (FBP) (OVARIAN TUMOR-ASSOCIATED ANTIGEN MOV18) (KB CELLS FBP).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
616.3706	616.3571	21.8828	26	30	(R)(T)
915.4830	915.4576	27.7529	77	83	(K)(F)
1295.6734	1295.6431	23.3215	53	61	(K)LHEQCRPWR(K)	1Cys-am
2276.0609	2276.0228	16.7234	209	227	(R)CIQMWFDPAQGNPNEEVAR(F)	1Cys-am
2292.0790	2292.0178	26.7324	209	227	(R)CIQMWFDPAQGNPNEEVAR(F)	1Met-ox 1Cys-am

8 unmatched masses: 1045.5922 1499.7218 1612.8631 1629.8471 1791.7560 2283.2266 2384.0575 2952.5767

The matched peptides cover 15% (40/257 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/13 matches (30%). 66948.2 Da, pI = 8.43. Acc. # P07358. HUMAN. COMPLEMENT COMPONENT C8 BETA CHAIN PRECURSOR.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5922 1045.5828 9.0096 403 411 (K)(D)
1499.7218 1499.7355 -9.1641 32 45 (R)(S)
1629.8471 1629.8859 -23.8159 403 415 (K)CRGILNEIKDRNK(R) 1Cys-am
1791.7560 1791.8118 -31.1308 106 121 (K)(C)

9 unmatched masses: 616.3706 915.4830 1295.6734 1612.8631 2276.0609 2283.2266 2292.0790 2384.0575 2952.5767

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5922	1045.5828	9.0096	403	411	(K)(D)
1499.7218	1499.7355	-9.1641	32	45	(R)(S)
1629.8471	1629.8859	-23.8159	403	415	(K)CRGILNEIKDRNK(R)	1Cys-am
1791.7560	1791.8118	-31.1308	106	121	(K)(C)

The matched peptides cover 7% (43/591 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/13 matches (30%). 27381.8 Da, pI = 9.56. Acc. # P31240. MOUSE. PLATELET-DERIVED GROWTH FACTOR, B CHAIN PRECURSOR (PDGF B-CHAIN) (PDGF-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1295.6734 1295.7224 -37.8783 226 236 (K)(E)
1499.7218 1499.7892 -44.9522 167 179 (K)KATVTLEDHLACK(C) 1Cys-am
1629.8471 1629.8522 -3.1396 82 98 (R)(T)
2952.5767 2952.6464 -23.6118 138 161 (R)NVQCRASQVQMRPVQVRKIEIVRK(K) 1Met-ox 1Cys-am

9 unmatched masses: 616.3706 915.4830 1045.5922 1612.8631 1791.7560 2276.0609 2283.2266 2292.0790 2384.0575

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1295.6734	1295.7224	-37.8783	226	236	(K)(E)
1499.7218	1499.7892	-44.9522	167	179	(K)KATVTLEDHLACK(C)	1Cys-am
1629.8471	1629.8522	-3.1396	82	98	(R)(T)
2952.5767	2952.6464	-23.6118	138	161	(R)NVQCRASQVQMRPVQVRKIEIVRK(K)	1Met-ox 1Cys-am

The matched peptides cover 26% (65/241 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/13 matches (30%). 58044.9 Da, pI = 9.75. Acc. # Q15696. HUMAN. U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR 35 KD SUBUNIT RELATED-PROTEIN 2.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
616.3706 616.3418 46.6360 32 36 (R)(L)
1629.8471 1629.8713 -14.8558 106 117 (R)(E)
2276.0609 2276.1684 -47.2193 1 19 (-)(A)
2292.0790 2292.1633 -36.7647 1 19 (-)MAAPEKMTFPEKPSHKKYR(A) 1Met-ox

9 unmatched masses: 915.4830 1045.5922 1295.6734 1499.7218 1612.8631 1791.7560 2283.2266 2384.0575 2952.5767

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
616.3706	616.3418	46.6360	32	36	(R)(L)
1629.8471	1629.8713	-14.8558	106	117	(R)(E)
2276.0609	2276.1684	-47.2193	1	19	(-)(A)
2292.0790	2292.1633	-36.7647	1	19	(-)MAAPEKMTFPEKPSHKKYR(A)	1Met-ox

The matched peptides cover 7% (36/482 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/13 matches (46%). 94300.5 Da, pI = 5.18. Acc. # P34932. HUMAN. HEAT SHOCK 70 KDA PROTEIN 4 (HEAT SHOCK 70-RELATED PROTEIN APG-2) (HSP70RY).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5922 1045.6257 -32.0291 323 331 (R)(K)
1045.5922 1045.6257 -32.0291 765 773 (K)(E)
1295.6734 1295.6959 -17.4349 186 196 (K)(N)
1499.7218 1499.7494 -18.4447 639 651 (K)(L)
1612.8631 1612.8845 -13.2578 558 571 (K)(V)
1612.8631 1612.9209 -35.8162 560 573 (K)KMDQPPQAKKAKVK(T) 1Met-ox
1629.8471 1629.9110 -39.2471 260 272 (K)IRALLRLSQECEK(L) 1Cys-am
1629.8471 1629.8674 -12.4999 705 717 (K)QIQQYMKIISSFK(N) 1Met-ox
2283.2266 2283.1266 43.8193 135 154 (K)(S)

7 unmatched masses: 616.3706 915.4830 1791.7560 2276.0609 2292.0790 2384.0575 2952.5767

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5922	1045.6257	-32.0291	323	331	(R)(K)
1045.5922	1045.6257	-32.0291	765	773	(K)(E)
1295.6734	1295.6959	-17.4349	186	196	(K)(N)
1499.7218	1499.7494	-18.4447	639	651	(K)(L)
1612.8631	1612.8845	-13.2578	558	571	(K)(V)
1612.8631	1612.9209	-35.8162	560	573	(K)KMDQPPQAKKAKVK(T)	1Met-ox
1629.8471	1629.9110	-39.2471	260	272	(K)IRALLRLSQECEK(L)	1Cys-am
1629.8471	1629.8674	-12.4999	705	717	(K)QIQQYMKIISSFK(N)	1Met-ox
2283.2266	2283.1266	43.8193	135	154	(K)(S)

The matched peptides cover 12% (104/840 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):