MS-Fit Search Results

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Sample ID (comment): HSG040
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 222 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
26

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.3e+003	7/26 (26%)	59134.9 / 6.44	HUMAN	GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE (EC 1.1.1.49) (G6PD).
2	185	4/26 (15%)	37643.7 / 6.72	HUMAN	CYCLIN H (MO15-ASSOCIATED PROTEIN) (P37) (P34).
3	110	5/26 (19%)	47699.1 / 7.20	HUMAN	TRANSCRIPTIONAL ENHANCER FACTOR TEF-3 (TRANSCRIPTION FACTOR RTEF-1).
4	97	4/26 (15%)	48028.7 / 8.12	MOUSE	TRANSCRIPTIONAL ENHANCER FACTOR TEF-3 (ETF-RELATED FACTOR-2) (ETFR- 2) (TEF-1-RELATED FACTOR 1) (TEF-1-RELATED FACTOR FR-19) (RTEF-1).
5	95	4/26 (15%)	49041.6 / 5.99	MOUSE	TRANSCRIPTIONAL ENHANCER FACTOR TEF-4 (EMBRYONIC TEA DOMAIN-CONTAINING FACTOR) (ETF) (ETEF-1) (TEAD-2).

Detailed Results

1. 7/26 matches (26%). 59134.9 Da, pI = 6.44. Acc. # P11413. HUMAN. GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE (EC 1.1.1.49) (G6PD)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1002.6002	1002.5525	47.5917	219	226	(R)(D)
1102.6492	1102.6049	40.1110	348	356	(R)(C)
1273.6651	1273.6217	34.0951	246	256	(R)(D)
1390.6848	1390.7306	-32.9314	348	359	(R)(A)
1664.8964	1664.8648	18.9915	57	71	(R)(S)
1807.8185	1807.7887	16.4814	104	119	(R)(L)
1928.0174	1927.9765	21.1813	330	347	(R)(W)

19 unmatched masses: 647.2810 661.3276 671.4575 733.4423 842.5438 899.6141 986.6471 1099.6592 1374.5830 1757.0227 1772.9645 1799.9408 1994.0085 2211.1147 2231.0685 2272.1716 2288.1530 2304.1686 2502.2390

The matched peptides cover 15% (80/514 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/26 matches (15%). 37643.7 Da, pI = 6.72. Acc. # P51946. HUMAN. CYCLIN H (MO15-ASSOCIATED PROTEIN) (P37) (P34)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
661.3276 661.3310 -5.1037 262 266 (K)(S)
1772.9645 1772.8814 46.8326 224 239 (R)(E)
1772.9645 1772.9547 5.5211 262 276 (K)(L)
1807.8185 1807.8979 -43.8972 115 130 (K)(E)
2211.1147 2211.0830 14.3320 40 58 (K)(Y)

22 unmatched masses: 647.2810 671.4575 733.4423 842.5438 899.6141 986.6471 1002.6002 1099.6592 1102.6492 1273.6651 1374.5830 1390.6848 1664.8964 1757.0227 1799.9408 1928.0174 1994.0085 2231.0685 2272.1716 2288.1530 2304.1686 2502.2390

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
661.3276	661.3310	-5.1037	262	266	(K)(S)
1772.9645	1772.8814	46.8326	224	239	(R)(E)
1772.9645	1772.9547	5.5211	262	276	(K)(L)
1807.8185	1807.8979	-43.8972	115	130	(K)(E)
2211.1147	2211.0830	14.3320	40	58	(K)(Y)

The matched peptides cover 20% (66/323 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/26 matches (19%). 47699.1 Da, pI = 7.20. Acc. # Q15561. HUMAN. TRANSCRIPTIONAL ENHANCER FACTOR TEF-3 (TRANSCRIPTION FACTOR RTEF-1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1002.6002 1002.5835 16.6447 58 66 (R)(M)
1099.6592 1099.6700 -9.8214 101 109 (R)(L)
2272.1716 2272.1470 10.8540 377 395 (K)(D)
2288.1530 2288.1419 4.8346 377 395 (K)YMMNSVLENFTILQVVTNR(D) 1Met-ox
2304.1686 2304.1368 13.7768 377 395 (K)YMMNSVLENFTILQVVTNR(D) 2Met-ox

21 unmatched masses: 647.2810 661.3276 671.4575 733.4423 842.5438 899.6141 986.6471 1102.6492 1273.6651 1374.5830 1390.6848 1664.8964 1757.0227 1772.9645 1799.9408 1807.8185 1928.0174 1994.0085 2211.1147 2231.0685 2502.2390

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1002.6002	1002.5835	16.6447	58	66	(R)(M)
1099.6592	1099.6700	-9.8214	101	109	(R)(L)
2272.1716	2272.1470	10.8540	377	395	(K)(D)
2288.1530	2288.1419	4.8346	377	395	(K)YMMNSVLENFTILQVVTNR(D)	1Met-ox
2304.1686	2304.1368	13.7768	377	395	(K)YMMNSVLENFTILQVVTNR(D)	2Met-ox

The matched peptides cover 8% (37/427 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/26 matches (15%). 48028.7 Da, pI = 8.12. Acc. # Q62296. MOUSE. TRANSCRIPTIONAL ENHANCER FACTOR TEF-3 (ETF-RELATED FACTOR-2) (ETFR- 2) (TEF-1-RELATED FACTOR 1) (TEF-1-RELATED FACTOR FR-19) (RTEF-1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1099.6592 1099.6700 -9.8214 101 109 (R)(L)
2272.1716 2272.1470 10.8540 377 395 (K)(D)
2288.1530 2288.1419 4.8346 377 395 (K)YMMNSVLENFTILQVVTNR(D) 1Met-ox
2304.1686 2304.1368 13.7768 377 395 (K)YMMNSVLENFTILQVVTNR(D) 2Met-ox

22 unmatched masses: 647.2810 661.3276 671.4575 733.4423 842.5438 899.6141 986.6471 1002.6002 1102.6492 1273.6651 1374.5830 1390.6848 1664.8964 1757.0227 1772.9645 1799.9408 1807.8185 1928.0174 1994.0085 2211.1147 2231.0685 2502.2390

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1099.6592	1099.6700	-9.8214	101	109	(R)(L)
2272.1716	2272.1470	10.8540	377	395	(K)(D)
2288.1530	2288.1419	4.8346	377	395	(K)YMMNSVLENFTILQVVTNR(D)	1Met-ox
2304.1686	2304.1368	13.7768	377	395	(K)YMMNSVLENFTILQVVTNR(D)	2Met-ox

The matched peptides cover 6% (28/427 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/26 matches (15%). 49041.6 Da, pI = 5.99. Acc. # P48301. MOUSE. TRANSCRIPTIONAL ENHANCER FACTOR TEF-4 (EMBRYONIC TEA DOMAIN-CONTAINING FACTOR) (ETF) (ETEF-1) (TEAD-2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1002.6002 1002.5835 16.6447 67 75 (R)(M)
2272.1716 2272.1470 10.8540 395 413 (R)(D)
2288.1530 2288.1419 4.8346 395 413 (R)YMMNSVLENFTILQVVTNR(D) 1Met-ox
2304.1686 2304.1368 13.7768 395 413 (R)YMMNSVLENFTILQVVTNR(D) 2Met-ox
2304.1686 2304.1070 26.7366 414 433 (R)(G)

22 unmatched masses: 647.2810 661.3276 671.4575 733.4423 842.5438 899.6141 986.6471 1099.6592 1102.6492 1273.6651 1374.5830 1390.6848 1664.8964 1757.0227 1772.9645 1799.9408 1807.8185 1928.0174 1994.0085 2211.1147 2231.0685 2502.2390

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1002.6002	1002.5835	16.6447	67	75	(R)(M)
2272.1716	2272.1470	10.8540	395	413	(R)(D)
2288.1530	2288.1419	4.8346	395	413	(R)YMMNSVLENFTILQVVTNR(D)	1Met-ox
2304.1686	2304.1368	13.7768	395	413	(R)YMMNSVLENFTILQVVTNR(D)	2Met-ox
2304.1686	2304.1070	26.7366	414	433	(R)(G)

The matched peptides cover 10% (48/445 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):