MS-Fit Search Results

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Sample ID (comment): HSG041
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 502 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
30

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.52e+006	17/30 (56%)	56782.7 / 5.99	HUMAN	PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR (EC 5.3.4.1) (ERP60) (58 KD MICROSOMAL PROTEIN) (P58) (GRP58) (ERP57).
2	832	10/30 (33%)	56621.6 / 5.99	MOUSE	PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR (EC 5.3.4.1) (ERP60) (58 KD MICROSOMAL PROTEIN) (P58).
3	430	7/30 (23%)	62630.6 / 9.16	MOUSE	ZINC FINGER PROTEIN ZFP-37 (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN).
4	412	6/30 (20%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
5	202	5/30 (16%)	6068.4 / 8.38	HUMAN	METALLOTHIONEIN-IL (MT-1L) (MT1X).

Detailed Results

1. 17/30 matches (56%). 56782.7 Da, pI = 5.99. Acc. # P30101. HUMAN. PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR (EC 5.3.4.1) (ERP60) (58 KD MICROSOMAL PROTEIN) (P58) (GRP58) (ERP57)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
782.3140	782.3572	-55.2454	141	146	(R)(K)
782.3140	782.3586	-56.9547	275	280	(K)(N)
1172.4943	1172.5410	-39.8131	336	344	(K)(D)
1179.5648	1179.5871	-18.9062	174	183	(K)(F)
1188.4921	1188.5359	-36.8690	336	344	(K)FVMQEEFSR(D)	1Met-ox
1191.5365	1191.6010	-54.1257	63	73	(R)(L)
1341.6307	1341.6843	-39.9967	449	460	(R)(K)
1359.5948	1359.6585	-46.8722	352	362	(R)(R)
1370.6481	1370.6956	-34.6306	472	482	(R)(E)
1515.6998	1515.7596	-39.4451	352	363	(R)(Y)
1619.8121	1619.7845	17.0414	259	271	(K)(N)
1664.7153	1664.7590	-26.2680	434	448	(K)(G)
1680.6774	1680.7539	-45.5311	434	448	(K)MDATANDVPSPYEVR(G)	1Met-ox
1832.8053	1832.9030	-53.3458	380	395	(K)(D)
1886.8978	1886.9976	-52.8897	130	146	(K)(K)
1886.8978	1886.9976	-52.8897	131	147	(K)(F)
2465.1822	2465.3161	-54.3269	283	304	(R)VMMVAKKFLDAGHKLNFAVASR(K)	2Met-ox
2575.1984	2575.3044	-41.1673	306	329	(K)(T)
2703.3019	2703.4703	-62.2842	281	304	(R)(K)
2703.3019	2703.3994	-36.0396	305	329	(R)(T)

13 unmatched masses: 568.1195 842.4904 1296.6570 1475.6964 1639.6791 1716.7811 1791.6337 1993.9316 2211.0161 2383.8589 2706.1469 2717.0124 2752.2364

The matched peptides cover 36% (182/505 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/30 matches (33%). 56621.6 Da, pI = 5.99. Acc. # P27773. MOUSE. PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR (EC 5.3.4.1) (ERP60) (58 KD MICROSOMAL PROTEIN) (P58)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
782.3140 782.3572 -55.2454 140 145 (R)(K)
782.3140 782.3586 -56.9547 274 279 (K)(N)
1172.4943 1172.5410 -39.8131 335 343 (K)(D)
1179.5648 1179.5871 -18.9062 173 182 (K)(F)
1188.4921 1188.5359 -36.8690 335 343 (K)FVMQEEFSR(D) 1Met-ox
1191.5365 1191.6010 -54.1257 63 73 (R)(L)
1341.6307 1341.6843 -39.9967 448 459 (K)(K)
1886.8978 1886.9976 -52.8897 129 145 (K)(K)
1886.8978 1886.9976 -52.8897 130 146 (K)(F)
2465.1822 2465.3161 -54.3269 282 303 (R)VMMVAKKFLDAGHKLNFAVASR(K) 2Met-ox
2575.1984 2575.2932 -36.8055 252 273 (K)(G)
2703.3019 2703.4703 -62.2842 280 303 (R)(K)

20 unmatched masses: 568.1195 842.4904 1296.6570 1359.5948 1370.6481 1475.6964 1515.6998 1619.8121 1639.6791 1664.7153 1680.6774 1716.7811 1791.6337 1832.8053 1993.9316 2211.0161 2383.8589 2706.1469 2717.0124 2752.2364

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
782.3140	782.3572	-55.2454	140	145	(R)(K)
782.3140	782.3586	-56.9547	274	279	(K)(N)
1172.4943	1172.5410	-39.8131	335	343	(K)(D)
1179.5648	1179.5871	-18.9062	173	182	(K)(F)
1188.4921	1188.5359	-36.8690	335	343	(K)FVMQEEFSR(D)	1Met-ox
1191.5365	1191.6010	-54.1257	63	73	(R)(L)
1341.6307	1341.6843	-39.9967	448	459	(K)(K)
1886.8978	1886.9976	-52.8897	129	145	(K)(K)
1886.8978	1886.9976	-52.8897	130	146	(K)(F)
2465.1822	2465.3161	-54.3269	282	303	(R)VMMVAKKFLDAGHKLNFAVASR(K)	2Met-ox
2575.1984	2575.2932	-36.8055	252	273	(K)(G)
2703.3019	2703.4703	-62.2842	280	303	(R)(K)

The matched peptides cover 22% (112/504 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/30 matches (23%). 62630.6 Da, pI = 9.16. Acc. # P17141. MOUSE. ZINC FINGER PROTEIN ZFP-37 (MALE GERM CELL SPECIFIC ZINC FINGER PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5648 1179.5945 -25.1740 365 374 (K)(I)
1370.6481 1370.6084 28.9585 407 417 (K)EKPYKCDECGK(A) 1Cys-am
1639.6791 1639.6303 29.7465 431 445 (R)(A)
1680.6774 1680.6998 -13.3174 347 361 (R)(A)
1680.6774 1680.7362 -34.9657 515 529 (R)(A)
1886.8978 1886.9936 -50.7580 154 170 (K)(K)
2383.8589 2384.0651 -86.4863 235 254 (R)THTGEKPYECNECGIAFSQK(S) 2Cys-am
2752.2364 2752.2724 -13.0857 479 501 (K)SHLIVHQRPHTGENPFECYECGK(A) 1Cys-am

23 unmatched masses: 568.1195 782.3140 842.4904 1172.4943 1188.4921 1191.5365 1296.6570 1341.6307 1359.5948 1475.6964 1515.6998 1619.8121 1664.7153 1716.7811 1791.6337 1832.8053 1993.9316 2211.0161 2465.1822 2575.1984 2703.3019 2706.1469 2717.0124

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.5648	1179.5945	-25.1740	365	374	(K)(I)
1370.6481	1370.6084	28.9585	407	417	(K)EKPYKCDECGK(A)	1Cys-am
1639.6791	1639.6303	29.7465	431	445	(R)(A)
1680.6774	1680.6998	-13.3174	347	361	(R)(A)
1680.6774	1680.7362	-34.9657	515	529	(R)(A)
1886.8978	1886.9936	-50.7580	154	170	(K)(K)
2383.8589	2384.0651	-86.4863	235	254	(R)THTGEKPYECNECGIAFSQK(S)	2Cys-am
2752.2364	2752.2724	-13.0857	479	501	(K)SHLIVHQRPHTGENPFECYECGK(A)	1Cys-am

The matched peptides cover 22% (126/553 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/30 matches (20%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.5648 1179.6010 -30.7055 376 385 (K)(H)
1475.6964 1475.7858 -60.5772 199 210 (R)(W)
1475.6964 1475.7494 -35.9231 211 222 (K)(T)
1716.7811 1716.8517 -41.0896 417 431 (K)(G)
1993.9316 1993.9772 -22.8502 223 238 (R)(R)
2383.8589 2383.9524 -39.2116 518 548 (R)(G)
2575.1984 2575.1405 22.4975 246 266 (K)SDQSRLDSELKNMQDMVEDYR(N) 1Met-ox

24 unmatched masses: 568.1195 782.3140 842.4904 1172.4943 1188.4921 1191.5365 1296.6570 1341.6307 1359.5948 1370.6481 1515.6998 1619.8121 1639.6791 1664.7153 1680.6774 1791.6337 1832.8053 1886.8978 2211.0161 2465.1822 2703.3019 2706.1469 2717.0124 2752.2364

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1179.5648	1179.6010	-30.7055	376	385	(K)(H)
1475.6964	1475.7858	-60.5772	199	210	(R)(W)
1475.6964	1475.7494	-35.9231	211	222	(K)(T)
1716.7811	1716.8517	-41.0896	417	431	(K)(G)
1993.9316	1993.9772	-22.8502	223	238	(R)(R)
2383.8589	2383.9524	-39.2116	518	548	(R)(G)
2575.1984	2575.1405	22.4975	246	266	(K)SDQSRLDSELKNMQDMVEDYR(N)	1Met-ox

The matched peptides cover 18% (117/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/30 matches (16%). 6068.4 Da, pI = 8.38. Acc. # P80297. HUMAN. METALLOTHIONEIN-IL (MT-1L) (MT1X)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1359.5948 1359.5352 43.8367 31 43 (K)KSCCSCCPVGCAK(C) 1Cys-am
1515.6998 1515.5887 73.3427 32 43 (K)SCCSCCPVGCAK(C) 5Cys-am
1993.9316 1993.7732 79.4467 44 61 (K)CAQGCICKGTSDKCSCCA(-) 3Cys-am
2383.8589 2383.9492 -37.8593 23 43 (K)ECKCTSCKKSCCSCCPVGCAK(C) 3Cys-am
2706.1469 2706.0583 32.7574 1 25 (-)DPNCSCSPVGSCACAGSCKCKECK(C) 4Cys-am Acet N

25 unmatched masses: 568.1195 782.3140 842.4904 1172.4943 1179.5648 1188.4921 1191.5365 1296.6570 1341.6307 1370.6481 1475.6964 1619.8121 1639.6791 1664.7153 1680.6774 1716.7811 1791.6337 1832.8053 1886.8978 2211.0161 2465.1822 2575.1984 2703.3019 2717.0124 2752.2364

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1359.5948	1359.5352	43.8367	31	43	(K)KSCCSCCPVGCAK(C)	1Cys-am
1515.6998	1515.5887	73.3427	32	43	(K)SCCSCCPVGCAK(C)	5Cys-am
1993.9316	1993.7732	79.4467	44	61	(K)CAQGCICKGTSDKCSCCA(-)	3Cys-am
2383.8589	2383.9492	-37.8593	23	43	(K)ECKCTSCKKSCCSCCPVGCAK(C)	3Cys-am
2706.1469	2706.0583	32.7574	1	25	(-)DPNCSCSPVGSCACAGSCKCKECK(C)	4Cys-am Acet N

The matched peptides cover 98% (60/61 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):