MS-Fit Search Results

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Sample ID (comment): HSG042
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 585 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
75.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
32

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.64e+004	14/32 (43%)	83163.5 / 4.97	HUMAN	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90).
2	3.31e+003	12/32 (37%)	83194.5 / 4.97	MOUSE	HEAT SHOCK PROTEIN HSP 84 (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA).
3	349	9/32 (28%)	59998.1 / 5.70	MOUSE	PROLYL 4-HYDROXYLASE ALPHA-1 SUBUNIT PRECURSOR (EC 1.14.11.2) (FRAGMENT).
4	292	10/32 (31%)	87960.1 / 6.00	HUMAN	ZINC FINGER PROTEIN 151 (MIZ-1 PROTEIN).
5	222	5/32 (15%)	27836.2 / 6.99	MOUSE	CALPAIN INHIBITOR (CALPASTATIN) (FRAGMENT).

Detailed Results

1. 14/32 matches (43%). 83163.5 Da, pI = 4.97. Acc. # P08238. HUMAN. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
701.4227	701.4562	-47.7476	197	202	(R)(K)
1045.5950	1045.6006	-5.3436	574	582	(K)(L)
1194.6994	1194.6483	42.7768	72	81	(K)(T)
1236.6631	1236.6377	20.5467	337	346	(R)(K)
1249.6831	1249.6177	52.3320	491	501	(K)(V)
1348.7192	1348.6650	40.1756	319	329	(K)(A)
1513.8548	1513.7862	45.2792	378	391	(R)(E)
1782.9482	1782.9503	-1.1300	624	638	(K)(Q)
1847.8151	1847.7976	9.4737	291	305	(R)(S)
1851.9639	1852.0076	-23.6219	559	573	(K)(V)
1994.0717	1994.0819	-5.1224	557	572	(K)AKFENLCKLMKEILDK(K)	1Cys-am
2256.1118	2255.9594	67.5777	148	167	(K)(A)
2384.0510	2384.0543	-1.4155	147	167	(R)(A)
2706.2603	2706.2764	-5.9388	583	606	(R)LVSSPCCIVTSTYGWTANMERIMK(A)	1Met-ox

18 unmatched masses: 825.1458 841.1010 842.5401 861.1007 877.0624 1179.6505 1307.7381 1475.7808 1638.9119 1707.8164 1766.8148 1838.0252 2097.0417 2211.2361 2233.1209 2234.1816 2284.2318 2299.2572

The matched peptides cover 22% (163/723 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/32 matches (37%). 83194.5 Da, pI = 4.97. Acc. # P11499. MOUSE. HEAT SHOCK PROTEIN HSP 84 (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
701.4227 701.4562 -47.7476 197 202 (R)(K)
1045.5950 1045.6006 -5.3436 574 582 (K)(L)
1194.6994 1194.6483 42.7768 72 81 (K)(T)
1236.6631 1236.6377 20.5467 337 346 (R)(K)
1348.7192 1348.6650 40.1756 319 329 (K)(A)
1513.8548 1513.7862 45.2792 378 391 (R)(E)
1782.9482 1782.9503 -1.1300 624 638 (K)(Q)
1847.8151 1847.7976 9.4737 291 305 (R)(S)
1851.9639 1852.0076 -23.6219 559 573 (K)(V)
1994.0717 1994.0819 -5.1224 557 572 (K)AKFENLCKLMKEILDK(K) 1Cys-am
2256.1118 2255.9594 67.5777 148 167 (K)(A)
2706.2603 2706.2764 -5.9388 583 606 (R)LVSSPCCIVTSTYGWTANMERIMK(A) 1Met-ox

20 unmatched masses: 825.1458 841.1010 842.5401 861.1007 877.0624 1179.6505 1249.6831 1307.7381 1475.7808 1638.9119 1707.8164 1766.8148 1838.0252 2097.0417 2211.2361 2233.1209 2234.1816 2284.2318 2299.2572 2384.0510

The matched peptides cover 20% (151/723 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/32 matches (28%). 59998.1 Da, pI = 5.70. Acc. # Q60715. MOUSE. PROLYL 4-HYDROXYLASE ALPHA-1 SUBUNIT PRECURSOR (EC 1.14.11.2) (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5950 1045.5795 14.8646 50 57 (K)(L)
1179.6505 1179.6308 16.6700 492 502 (R)HAACPVLVGNK(W) 1Cys-am
1249.6831 1249.6541 23.2140 223 232 (K)(A)
1513.8548 1513.7440 73.1972 65 78 (K)(L)
1638.9119 1638.7975 69.8329 129 142 (R)(G)
1707.8164 1707.7786 22.1777 247 262 (K)(T)
1847.8151 1847.8735 -31.6329 249 266 (K)(K)
2233.1209 2233.1060 6.6581 247 267 (K)(K)
2299.2572 2299.1473 47.7772 279 296 (R)QKYEMLCRGEGIKMTPRR(Q) 2Met-ox 1Cys-am
2299.2572 2299.1473 47.7772 281 298 (K)YEMLCRGEGIKMTPRRQK(R) 2Met-ox 1Cys-am

23 unmatched masses: 701.4227 825.1458 841.1010 842.5401 861.1007 877.0624 1194.6994 1236.6631 1307.7381 1348.7192 1475.7808 1766.8148 1782.9482 1838.0252 1851.9639 1994.0717 2097.0417 2211.2361 2234.1816 2256.1118 2284.2318 2384.0510 2706.2603

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1045.5950	1045.5795	14.8646	50	57	(K)(L)
1179.6505	1179.6308	16.6700	492	502	(R)HAACPVLVGNK(W)	1Cys-am
1249.6831	1249.6541	23.2140	223	232	(K)(A)
1513.8548	1513.7440	73.1972	65	78	(K)(L)
1638.9119	1638.7975	69.8329	129	142	(R)(G)
1707.8164	1707.7786	22.1777	247	262	(K)(T)
1847.8151	1847.8735	-31.6329	249	266	(K)(K)
2233.1209	2233.1060	6.6581	247	267	(K)(K)
2299.2572	2299.1473	47.7772	279	296	(R)QKYEMLCRGEGIKMTPRR(Q)	2Met-ox 1Cys-am
2299.2572	2299.1473	47.7772	281	298	(K)YEMLCRGEGIKMTPRRQK(R)	2Met-ox 1Cys-am

The matched peptides cover 18% (98/526 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/32 matches (31%). 87960.1 Da, pI = 6.00. Acc. # Q13105. HUMAN. ZINC FINGER PROTEIN 151 (MIZ-1 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1236.6631 1236.6125 40.8867 314 323 (K)(H)
1766.8148 1766.7815 18.8696 383 397 (K)(L)
1766.8148 1766.9264 -63.1142 604 618 (K)(C)
1838.0252 1837.9635 33.5905 604 618 (K)HIIIHTGEKPYLCDK(C) 1Cys-am
1994.0717 1993.9880 41.9691 19 36 (R)(A)
2097.0417 2096.9745 32.0653 296 313 (R)TESKAYGSVIHKCEDCGK(E) 2Cys-am
2097.0417 2096.9978 20.9370 520 537 (R)HVRIHTGEKPCQCVMCGK(A) 1Cys-am
2233.1209 2233.0858 15.7313 390 407 (R)YRCEDCGKLFTTSGNLKR(H) 2Cys-am
2234.1816 2234.1399 18.6886 476 494 (K)(I)
2256.1118 2256.0541 25.5684 351 369 (K)AHEKTHSPLKPYGCEECGK(S) 2Cys-am
2284.2318 2284.2373 -2.4243 142 163 (K)(D)
2284.2318 2284.1555 33.3869 323 341 (K)(A)
2299.2572 2299.1166 61.1433 113 136 (K)(A)

22 unmatched masses: 701.4227 825.1458 841.1010 842.5401 861.1007 877.0624 1045.5950 1179.6505 1194.6994 1249.6831 1307.7381 1348.7192 1475.7808 1513.8548 1638.9119 1707.8164 1782.9482 1847.8151 1851.9639 2211.2361 2384.0510 2706.2603

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1236.6631	1236.6125	40.8867	314	323	(K)(H)
1766.8148	1766.7815	18.8696	383	397	(K)(L)
1766.8148	1766.9264	-63.1142	604	618	(K)(C)
1838.0252	1837.9635	33.5905	604	618	(K)HIIIHTGEKPYLCDK(C)	1Cys-am
1994.0717	1993.9880	41.9691	19	36	(R)(A)
2097.0417	2096.9745	32.0653	296	313	(R)TESKAYGSVIHKCEDCGK(E)	2Cys-am
2097.0417	2096.9978	20.9370	520	537	(R)HVRIHTGEKPCQCVMCGK(A)	1Cys-am
2233.1209	2233.0858	15.7313	390	407	(R)YRCEDCGKLFTTSGNLKR(H)	2Cys-am
2234.1816	2234.1399	18.6886	476	494	(K)(I)
2256.1118	2256.0541	25.5684	351	369	(K)AHEKTHSPLKPYGCEECGK(S)	2Cys-am
2284.2318	2284.2373	-2.4243	142	163	(K)(D)
2284.2318	2284.1555	33.3869	323	341	(K)(A)
2299.2572	2299.1166	61.1433	113	136	(K)(A)

The matched peptides cover 25% (206/803 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/32 matches (15%). 27836.2 Da, pI = 6.99. Acc. # P51125. MOUSE. CALPAIN INHIBITOR (CALPASTATIN) (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
701.4227 701.4310 -11.8910 28 34 (K)(E)
1045.5950 1045.6006 -5.3436 254 261 (K)(-)
2234.1816 2234.0941 39.1903 79 102 (K)(V)
2256.1118 2256.1512 -17.4505 109 129 (K)(S)
2706.2603 2706.3224 -22.9600 152 176 (R)(E)

27 unmatched masses: 825.1458 841.1010 842.5401 861.1007 877.0624 1179.6505 1194.6994 1236.6631 1249.6831 1307.7381 1348.7192 1475.7808 1513.8548 1638.9119 1707.8164 1766.8148 1782.9482 1838.0252 1847.8151 1851.9639 1994.0717 2097.0417 2211.2361 2233.1209 2284.2318 2299.2572 2384.0510

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
701.4227	701.4310	-11.8910	28	34	(K)(E)
1045.5950	1045.6006	-5.3436	254	261	(K)(-)
2234.1816	2234.0941	39.1903	79	102	(K)(V)
2256.1118	2256.1512	-17.4505	109	129	(K)(S)
2706.2603	2706.3224	-22.9600	152	176	(R)(E)

The matched peptides cover 32% (84/261 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):