MS-Fit Search Results

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Sample ID (comment): HSG043
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 357 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
29

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	3.08e+006	16/29 (55%)	35076.9 / 7.60	HUMAN	GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN 12.3 (P205) (RECEPTOR OF ACTIVATED PROTEIN KINASE C 1) (RACK1).
2	164	5/29 (17%)	47570.8 / 4.75	HUMAN	KERATIN, TYPE I CUTICULAR HA5 (HAIR KERATIN, TYPE I HA5).
3	100	5/29 (17%)	50131.0 / 6.68	MOUSE	MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II).
3	99.8	4/29 (13%)	45829.1 / 4.73	HUMAN	KERATIN, TYPE I CUTICULAR HA2 (HAIR KERATIN, TYPE I HA2).
4	97.4	4/29 (13%)	15230.5 / 5.59	HUMAN	MYOTUBULARIN-RELATED PROTEIN 1 (FRAGMENT).

Detailed Results

1. 16/29 matches (55%). 35076.9 Da, pI = 7.60. Acc. # P25388. HUMAN. GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN 12.3 (P205) (RECEPTOR OF ACTIVATED PROTEIN KINASE C 1) (RACK1)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
860.4749	860.4518	26.8478	258	264	(K)(I)
936.4720	936.4461	27.6839	1	8	(-)TEQMTLR(G)	1Met-ox Acet N
1018.5568	1018.5144	41.5774	176	183	(K)VWNLANCK(L)	1Cys-am
1059.6414	1059.5951	43.6950	309	317	(R)(-)
1192.6085	1192.5598	40.7630	48	57	(R)(A)
1264.7088	1264.6537	43.5422	89	99	(R)(R)
1309.7033	1309.6388	49.2140	107	118	(K)(Q)
1309.7033	1309.6615	31.9189	246	257	(R)(I)
1492.7182	1492.6742	29.4923	213	225	(K)DGQAMLWDLNEGK(H)	1Met-ox
1562.8429	1562.7927	32.1282	45	57	(K)(A)
1789.0495	1788.9959	29.9836	265	280	(K)(A)
1922.0005	1921.9118	46.1539	156	172	(R)FSPNSSNPIIVSCGWDK(L)	1Cys-am
1995.9583	1995.8871	35.7129	140	155	(K)YTVQDESHSEWVSCVR(F)	1Cys-am
2627.3916	2627.3040	33.3398	13	36	(K)(D)
2643.3498	2643.2989	19.2261	13	36	(K)GHNGWVTQIATTPQFPDMILSASR(D)	1Met-ox
2961.5345	2961.4131	40.9980	61	88	(R)(L)
2961.5345	2961.5800	-15.3439	246	271	(R)(Q)
3082.5309	3082.4580	23.6544	281	308	(K)AEPPQCTSLAWSADGQTLFAGYTDNLVR(V)	1Cys-am

13 unmatched masses: 753.3280 825.1674 842.5456 920.5029 1045.6330 1475.8017 1791.8216 2233.1742 2291.1626 2384.0526 2644.3425 2963.4746 3080.5136

The matched peptides cover 69% (221/317 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/29 matches (17%). 47570.8 Da, pI = 4.75. Acc. # Q92764. HUMAN. KERATIN, TYPE I CUTICULAR HA5 (HAIR KERATIN, TYPE I HA5)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
860.4749 860.4729 2.2916 120 126 (R)(K)
1995.9583 1996.0398 -40.8177 288 304 (R)(D)
2233.1742 2233.1637 4.7011 176 195 (R)(E)
2627.3916 2627.2632 48.8992 318 340 (R)(A)
2643.3498 2643.2581 34.6911 318 340 (R)YSSQLAQMQCMITNVEAQLAEIR(A) 1Met-ox

24 unmatched masses: 753.3280 825.1674 842.5456 920.5029 936.4720 1018.5568 1045.6330 1059.6414 1192.6085 1264.7088 1309.7033 1475.8017 1492.7182 1562.8429 1789.0495 1791.8216 1922.0005 2291.1626 2384.0526 2644.3425 2961.5345 2963.4746 3080.5136 3082.5309

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
860.4749	860.4729	2.2916	120	126	(R)(K)
1995.9583	1996.0398	-40.8177	288	304	(R)(D)
2233.1742	2233.1637	4.7011	176	195	(R)(E)
2627.3916	2627.2632	48.8992	318	340	(R)(A)
2643.3498	2643.2581	34.6911	318	340	(R)YSSQLAQMQCMITNVEAQLAEIR(A)	1Met-ox

The matched peptides cover 15% (67/425 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/29 matches (17%). 50131.0 Da, pI = 6.68. Acc. # P30204. MOUSE. MACROPHAGE SCAVENGER RECEPTOR TYPES I AND II (MACROPHAGE ACETYLATED LDL RECEPTOR I AND II)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
860.4749 860.4478 31.5231 137 144 (K)(F)
936.4720 936.4395 34.6522 87 93 (K)NCLVCSR(N) 2Cys-am
936.4720 936.5155 -46.4228 219 225 (K)(V)
2291.1626 2291.0854 33.7212 410 429 (R)AHFGQGTGPIWLNEVMCFGR(E) 1Cys-am
2627.3916 2627.3880 1.3662 386 408 (R)(R)
2643.3498 2643.3630 -5.0011 223 245 (R)(Q)

24 unmatched masses: 753.3280 825.1674 842.5456 920.5029 1018.5568 1045.6330 1059.6414 1192.6085 1264.7088 1309.7033 1475.8017 1492.7182 1562.8429 1789.0495 1791.8216 1922.0005 1995.9583 2233.1742 2384.0526 2644.3425 2961.5345 2963.4746 3080.5136 3082.5309

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
860.4749	860.4478	31.5231	137	144	(K)(F)
936.4720	936.4395	34.6522	87	93	(K)NCLVCSR(N)	2Cys-am
936.4720	936.5155	-46.4228	219	225	(K)(V)
2291.1626	2291.0854	33.7212	410	429	(R)AHFGQGTGPIWLNEVMCFGR(E)	1Cys-am
2627.3916	2627.3880	1.3662	386	408	(R)(R)
2643.3498	2643.3630	-5.0011	223	245	(R)(Q)

The matched peptides cover 18% (85/458 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/29 matches (13%). 45829.1 Da, pI = 4.73. Acc. # Q14532. HUMAN. KERATIN, TYPE I CUTICULAR HA2 (HAIR KERATIN, TYPE I HA2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
936.4720 936.4535 19.7884 182 188 (K)EELMCLK(K) 1Cys-am
1791.8216 1791.9063 -47.3188 124 139 (R)(A)
2627.3916 2627.2632 48.8992 304 326 (R)(A)
2643.3498 2643.2581 34.6911 304 326 (R)YSSQLAQMQCMITNVEAQLAEIR(A) 1Met-ox

25 unmatched masses: 753.3280 825.1674 842.5456 860.4749 920.5029 1018.5568 1045.6330 1059.6414 1192.6085 1264.7088 1309.7033 1475.8017 1492.7182 1562.8429 1789.0495 1922.0005 1995.9583 2233.1742 2291.1626 2384.0526 2644.3425 2961.5345 2963.4746 3080.5136 3082.5309

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
936.4720	936.4535	19.7884	182	188	(K)EELMCLK(K)	1Cys-am
1791.8216	1791.9063	-47.3188	124	139	(R)(A)
2627.3916	2627.2632	48.8992	304	326	(R)(A)
2643.3498	2643.2581	34.6911	304	326	(R)YSSQLAQMQCMITNVEAQLAEIR(A)	1Met-ox

The matched peptides cover 11% (46/405 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/29 matches (13%). 15230.5 Da, pI = 5.59. Acc. # Q13613. HUMAN. MYOTUBULARIN-RELATED PROTEIN 1 (FRAGMENT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1264.7088 1264.7153 -5.1172 59 69 (R)(E)
2627.3916 2627.4132 -8.2068 1 23 (-)(I)
2643.3498 2643.4081 -22.0686 1 23 (-)VDGTHWLEYIRMLLAGAVRIADK(I) 1Met-ox
2644.3425 2644.3339 3.2479 111 132 (R)(-)

25 unmatched masses: 753.3280 825.1674 842.5456 860.4749 920.5029 936.4720 1018.5568 1045.6330 1059.6414 1192.6085 1309.7033 1475.8017 1492.7182 1562.8429 1789.0495 1791.8216 1922.0005 1995.9583 2233.1742 2291.1626 2384.0526 2961.5345 2963.4746 3080.5136 3082.5309

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1264.7088	1264.7153	-5.1172	59	69	(R)(E)
2627.3916	2627.4132	-8.2068	1	23	(-)(I)
2643.3498	2643.4081	-22.0686	1	23	(-)VDGTHWLEYIRMLLAGAVRIADK(I)	1Met-ox
2644.3425	2644.3339	3.2479	111	132	(R)(-)

The matched peptides cover 41% (55/132 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):