MS-Fit Search Results

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Sample ID (comment): HSG045
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 632 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
5

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
45

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	7.69e+003	11/45 (24%)	27692.8 / 7.67	HUMAN	MITOCHONDRIAL THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE PRECURSOR (ANTIOXIDANT PROTEIN 1) (AOP-1) (MER5 PROTEIN HOMOLOG) (HBC189).
2	3.3e+003	10/45 (22%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
3	306	4/45 (8%)	28357.7 / 5.05	HUMAN	C4B-BINDING PROTEIN BETA CHAIN PRECURSOR.
4	220	4/45 (8%)	11342.5 / 10.40	HUMAN	MACROPHAGE INFLAMMATORY PROTEIN-2-BETA PRECURSOR (MIP2-BETA) (GROWTH REGULATED PROTEIN GAMMA) (GRO-GAMMA).
5	201	10/45 (22%)	87960.1 / 6.00	HUMAN	ZINC FINGER PROTEIN 151 (MIZ-1 PROTEIN).

Detailed Results

1. 11/45 matches (24%). 27692.8 Da, pI = 7.67. Acc. # P30048. HUMAN. MITOCHONDRIAL THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE PRECURSOR (ANTIOXIDANT PROTEIN 1) (AOP-1) (MER5 PROTEIN HOMOLOG) (HBC189)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
714.3112	714.3463	-49.1162	249	253	(K)(V)
833.4149	833.4369	-26.3931	208	214	(R)(L)
1206.6352	1206.6595	-20.0953	197	207	(K)(S)
1285.7278	1285.7520	-18.7996	185	196	(R)(H)
1462.7661	1462.7906	-16.7482	171	184	(R)(G)
1753.8601	1753.9271	-38.1814	150	166	(K)(Q)
1769.8566	1769.9220	-36.9197	150	166	(K)NGGLGHMNIALLSDLTK(Q)	1Met-ox
1881.9842	1882.0220	-20.1079	149	166	(R)(Q)
1954.8787	1954.9762	-49.8787	74	91	(K)(G)
3399.5684	3399.6320	-18.7058	218	248	(K)AFQYVETHGEVCPANWTPDSPTIKPSPAASK(E)	1Cys-am
3464.6091	3464.6446	-10.2505	119	148	(K)ANEFHDVNCEVVAVSVDSHFSHLAWINTPR(K)	1Cys-am

34 unmatched masses: 633.2291 647.2244 842.4961 1045.5384 1179.5680 1258.6416 1274.5872 1307.6768 1316.6344 1329.6763 1350.6223 1475.7369 1487.7000 1557.8061 1563.6695 1638.8204 1650.7688 1657.7520 1707.7190 1716.8060 1766.7872 1837.8694 1851.8062 1898.9634 1940.8739 1993.9358 2096.8775 2150.0598 2211.0709 2283.1308 2383.8922 2705.0978 2718.0414 3150.3576

The matched peptides cover 57% (146/256 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/45 matches (22%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5384 1045.5754 -35.4515 177 184 (K)(S)
1179.5680 1179.6010 -27.9148 376 385 (K)(H)
1475.7369 1475.7858 -33.1335 199 210 (R)(W)
1475.7369 1475.7494 -8.4787 211 222 (K)(T)
1638.8204 1638.8604 -24.3907 185 198 (K)(F)
1657.7520 1657.7934 -24.9619 12 28 (R)(R)
1716.8060 1716.8517 -26.5980 417 431 (K)(G)
1881.9842 1881.9711 6.9854 181 196 (R)(V)
1993.9358 1993.9772 -20.7865 223 238 (R)(R)
2150.0598 2150.0783 -8.6112 223 239 (R)(R)
2383.8922 2383.9524 -25.2701 518 548 (R)(G)

35 unmatched masses: 633.2291 647.2244 714.3112 833.4149 842.4961 1206.6352 1258.6416 1274.5872 1285.7278 1307.6768 1316.6344 1329.6763 1350.6223 1462.7661 1487.7000 1557.8061 1563.6695 1650.7688 1707.7190 1753.8601 1766.7872 1769.8566 1837.8694 1851.8062 1898.9634 1940.8739 1954.8787 2096.8775 2211.0709 2283.1308 2705.0978 2718.0414 3150.3576 3399.5684 3464.6091

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5384	1045.5754	-35.4515	177	184	(K)(S)
1179.5680	1179.6010	-27.9148	376	385	(K)(H)
1475.7369	1475.7858	-33.1335	199	210	(R)(W)
1475.7369	1475.7494	-8.4787	211	222	(K)(T)
1638.8204	1638.8604	-24.3907	185	198	(K)(F)
1657.7520	1657.7934	-24.9619	12	28	(R)(R)
1716.8060	1716.8517	-26.5980	417	431	(K)(G)
1881.9842	1881.9711	6.9854	181	196	(R)(V)
1993.9358	1993.9772	-20.7865	223	238	(R)(R)
2150.0598	2150.0783	-8.6112	223	239	(R)(R)
2383.8922	2383.9524	-25.2701	518	548	(R)(G)

The matched peptides cover 21% (136/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/45 matches (8%). 28357.7 Da, pI = 5.05. Acc. # P20851. HUMAN. C4B-BINDING PROTEIN BETA CHAIN PRECURSOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1766.7872 1766.7502 20.9645 1 14 (-)(V)
1769.8566 1769.8025 30.6116 214 227 (K)NLCEAMENFMQQLK(E) 1Cys-am
1837.8694 1837.7873 44.6853 1 14 (-)MFFWCACCLMVAWR(V) 1Cys-am
1940.8739 1940.8142 30.7423 1 14 (-)MFFWCACCLMVAWR(V) 2Met-ox 2Cys-am

41 unmatched masses: 633.2291 647.2244 714.3112 833.4149 842.4961 1045.5384 1179.5680 1206.6352 1258.6416 1274.5872 1285.7278 1307.6768 1316.6344 1329.6763 1350.6223 1462.7661 1475.7369 1487.7000 1557.8061 1563.6695 1638.8204 1650.7688 1657.7520 1707.7190 1716.8060 1753.8601 1851.8062 1881.9842 1898.9634 1954.8787 1993.9358 2096.8775 2150.0598 2211.0709 2283.1308 2383.8922 2705.0978 2718.0414 3150.3576 3399.5684 3464.6091

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1766.7872	1766.7502	20.9645	1	14	(-)(V)
1769.8566	1769.8025	30.6116	214	227	(K)NLCEAMENFMQQLK(E)	1Cys-am
1837.8694	1837.7873	44.6853	1	14	(-)MFFWCACCLMVAWR(V)	1Cys-am
1940.8739	1940.8142	30.7423	1	14	(-)MFFWCACCLMVAWR(V)	2Met-ox 2Cys-am

The matched peptides cover 11% (28/252 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/45 matches (8%). 11342.5 Da, pI = 10.40. Acc. # P19876. HUMAN. MACROPHAGE INFLAMMATORY PROTEIN-2-BETA PRECURSOR (MIP2-BETA) (GROWTH REGULATED PROTEIN GAMMA) (GRO-GAMMA)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1258.6416 1258.6288 10.1923 84 95 (K)(I)
1274.5872 1274.6237 -28.6393 84 95 (K)ACLNPASPMVQK(I) 1Met-ox
1329.6763 1329.6659 7.8166 84 95 (K)ACLNPASPMVQK(I) 1Cys-am
2150.0598 2150.1392 -36.9281 64 83 (R)SPGPHCAQTEVIATLKNGKK(A) 1Cys-am

41 unmatched masses: 633.2291 647.2244 714.3112 833.4149 842.4961 1045.5384 1179.5680 1206.6352 1285.7278 1307.6768 1316.6344 1350.6223 1462.7661 1475.7369 1487.7000 1557.8061 1563.6695 1638.8204 1650.7688 1657.7520 1707.7190 1716.8060 1753.8601 1766.7872 1769.8566 1837.8694 1851.8062 1881.9842 1898.9634 1940.8739 1954.8787 1993.9358 2096.8775 2211.0709 2283.1308 2383.8922 2705.0978 2718.0414 3150.3576 3399.5684 3464.6091

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1258.6416	1258.6288	10.1923	84	95	(K)(I)
1274.5872	1274.6237	-28.6393	84	95	(K)ACLNPASPMVQK(I)	1Met-ox
1329.6763	1329.6659	7.8166	84	95	(K)ACLNPASPMVQK(I)	1Cys-am
2150.0598	2150.1392	-36.9281	64	83	(R)SPGPHCAQTEVIATLKNGKK(A)	1Cys-am

The matched peptides cover 29% (32/107 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/45 matches (22%). 87960.1 Da, pI = 6.00. Acc. # Q13105. HUMAN. ZINC FINGER PROTEIN 151 (MIZ-1 PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1274.5872 1274.6316 -34.8076 327 337 (R)(E)
1557.8061 1557.8614 -35.4762 482 494 (K)(I)
1766.7872 1766.7815 3.2418 383 397 (K)(L)
1769.8566 1769.8580 -0.7373 495 509 (R)(Q)
1837.8694 1837.8186 27.6473 383 397 (K)RHSGEARYRCEDCGK(L) 1Cys-am
1898.9634 1898.9329 16.0349 476 491 (K)CRECGKQFTTSGNLKR(H) 1Cys-am
1954.8787 1954.9003 -11.0422 296 313 (R)(E)
1993.9358 1993.9880 -26.1848 19 36 (R)(A)
2096.8775 2096.9745 -46.2762 296 313 (R)TESKAYGSVIHKCEDCGK(E) 2Cys-am
2150.0598 2150.0666 -3.1685 40 57 (K)AVLAACSEYFKMLFVDQK(D) 1Met-ox 1Cys-am

35 unmatched masses: 633.2291 647.2244 714.3112 833.4149 842.4961 1045.5384 1179.5680 1206.6352 1258.6416 1285.7278 1307.6768 1316.6344 1329.6763 1350.6223 1462.7661 1475.7369 1487.7000 1563.6695 1638.8204 1650.7688 1657.7520 1707.7190 1716.8060 1753.8601 1851.8062 1881.9842 1940.8739 2211.0709 2283.1308 2383.8922 2705.0978 2718.0414 3150.3576 3399.5684 3464.6091

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1274.5872	1274.6316	-34.8076	327	337	(R)(E)
1557.8061	1557.8614	-35.4762	482	494	(K)(I)
1766.7872	1766.7815	3.2418	383	397	(K)(L)
1769.8566	1769.8580	-0.7373	495	509	(R)(Q)
1837.8694	1837.8186	27.6473	383	397	(K)RHSGEARYRCEDCGK(L)	1Cys-am
1898.9634	1898.9329	16.0349	476	491	(K)CRECGKQFTTSGNLKR(H)	1Cys-am
1954.8787	1954.9003	-11.0422	296	313	(R)(E)
1993.9358	1993.9880	-26.1848	19	36	(R)(A)
2096.8775	2096.9745	-46.2762	296	313	(R)TESKAYGSVIHKCEDCGK(E)	2Cys-am
2150.0598	2150.0666	-3.1685	40	57	(K)AVLAACSEYFKMLFVDQK(D)	1Met-ox 1Cys-am

The matched peptides cover 14% (114/803 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):