MS-Fit Search Results

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Sample ID (comment): HSG047
Database searched: SwissProt.6.17.2000
Molecular weight search (1000 - 100000 Da) selects 82508 entries.
Full pI range: 87272 entries.
Species search ( HUMAN MOUSE ) selects 9626 entries.
Combined molecular weight, pI and species searches select 8686 entries.
MS-Fit search selects 70 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
30

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.28e+003	9/30 (30%)	22327.3 / 7.83	HUMAN	HEAT SHOCK 27 KDA PROTEIN (HSP 27) (STRESS-RESPONSIVE PROTEIN 27) (SRP27) (ESTROGEN-REGULATED 24 KDA PROTEIN) (28 KDA HEAT SHOCK PROTEIN)
2	109	5/30 (16%)	31371.6 / 8.34	HUMAN	ENOYL-COA HYDRATASE, MITOCHONDRIAL PRECURSOR (SHORT CHAIN ENOYL-COA HYDRATASE) (SCEH) (ENOYL-COA HYDRATASE 1)
3	28	4/30 (13%)	29455.8 / 10.25	HUMAN	40S RIBOSOMAL PROTEIN S4, Y ISOFORM
4	26.4	4/30 (13%)	57315.3 / 9.86	MOUSE	CYTOCHROME P450 11B2 PRECURSOR (CYPXIB2) (P450C11) (STEROID 11-BETA-HYDROXYLASE) (ALDOSTERONE SYNTHASE)
5	21	4/30 (13%)	45170.4 / 6.17	MOUSE	PLASMINOGEN ACTIVATOR INHIBITOR-1 PRECURSOR (PAI-1)

Detailed Results

1. 9/30 matches (30%). 22327.3 Da, pI = 7.83. Acc. # P04792. HUMAN. HEAT SHOCK 27 KDA PROTEIN (HSP 27) (STRESS-RESPONSIVE PROTEIN 27) (SRP27) (ESTROGEN-REGULATED 24 KDA PROTEIN) (28 KDA HEAT SHOCK PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
831.4856	831.5092	-28.3918	6	12	(R)(G)
941.4568	941.4706	-14.6453	90	96	(R)(V)
987.5949	987.6104	-15.6723	5	12	(R)(G)
1075.5580	1075.5748	-15.5698	80	89	(R)(H)
1163.6184	1163.6213	-2.5006	28	37	(R)(L)
1655.8056	1655.8465	-24.6876	80	94	(R)(W)
1655.8056	1655.7526	32.0227	124	136	(K)(C)
1783.9240	1783.9230	0.5343	97	112	(R)(T)
1902.8997	1902.8676	16.8842	13	27	(R)(L)
1906.0066	1905.9922	7.5823	172	188	(K)(A)

21 unmatched masses: 525.1511 541.1696 543.1467 568.0932 570.1851 588.2028 599.3417 619.2383 647.2154 649.9970 665.9847 675.2422 842.4792 971.3705 1463.7250 1466.8225 1568.8232 1651.9670 1886.6416 2125.1812 2211.1992

The matched peptides cover 48% (96/199 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/30 matches (16%). 31371.6 Da, pI = 8.34. Acc. # P30084. HUMAN. ENOYL-COA HYDRATASE, MITOCHONDRIAL PRECURSOR (SHORT CHAIN ENOYL-COA HYDRATASE) (SCEH) (ENOYL-COA HYDRATASE 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1163.6184 1163.5962 19.1152 119 127 (K)(K)
1463.7250 1463.7171 5.3851 261 272 (K)(K)
1463.7250 1463.7171 5.3851 262 273 (K)(E)
1466.8225 1466.8443 -14.9143 44 56 (K)(A)
1651.9670 1651.9608 3.7975 42 56 (R)(A)
2125.1812 2125.1406 19.1062 158 178 (K)(L)

25 unmatched masses: 525.1511 541.1696 543.1467 568.0932 570.1851 588.2028 599.3417 619.2383 647.2154 649.9970 665.9847 675.2422 831.4856 842.4792 941.4568 971.3705 987.5949 1075.5580 1568.8232 1655.8056 1783.9240 1886.6416 1902.8997 1906.0066 2211.1992

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1163.6184	1163.5962	19.1152	119	127	(K)(K)
1463.7250	1463.7171	5.3851	261	272	(K)(K)
1463.7250	1463.7171	5.3851	262	273	(K)(E)
1466.8225	1466.8443	-14.9143	44	56	(K)(A)
1651.9670	1651.9608	3.7975	42	56	(R)(A)
2125.1812	2125.1406	19.1062	158	178	(K)(L)

The matched peptides cover 20% (58/290 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/30 matches (13%). 29455.8 Da, pI = 10.25. Acc. # P22090. HUMAN. 40S RIBOSOMAL PROTEIN S4, Y ISOFORM.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
831.4856 831.4616 28.8779 149 155 (R)(V)
831.4856 831.5052 -23.5540 253 259 (R)(Q)
842.4792 842.4749 5.0540 107 113 (K)(I)
1906.0066 1906.0883 -42.8387 63 77 (K)KICMQRFIKIDGKVR(V) 1Cys-am
2125.1812 2125.1922 -5.1940 222 240 (R)(G)

26 unmatched masses: 525.1511 541.1696 543.1467 568.0932 570.1851 588.2028 599.3417 619.2383 647.2154 649.9970 665.9847 675.2422 941.4568 971.3705 987.5949 1075.5580 1163.6184 1463.7250 1466.8225 1568.8232 1651.9670 1655.8056 1783.9240 1886.6416 1902.8997 2211.1992

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
831.4856	831.4616	28.8779	149	155	(R)(V)
831.4856	831.5052	-23.5540	253	259	(R)(Q)
842.4792	842.4749	5.0540	107	113	(K)(I)
1906.0066	1906.0883	-42.8387	63	77	(K)KICMQRFIKIDGKVR(V)	1Cys-am
2125.1812	2125.1922	-5.1940	222	240	(R)(G)

The matched peptides cover 20% (55/263 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/30 matches (13%). 57315.3 Da, pI = 9.86. Acc. # P15539. MOUSE. CYTOCHROME P450 11B2 PRECURSOR (CYPXIB2) (P450C11) (STEROID 11-BETA-HYDROXYLASE) (ALDOSTERONE SYNTHASE).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
831.4856 831.4729 15.3673 74 80 (R)(H)
987.5949 987.5549 40.5074 358 366 (K)(A)
1466.8225 1466.8807 -39.7186 144 156 (R)(F)
1906.0066 1906.0148 -4.3010 484 500 (R)(-)

26 unmatched masses: 525.1511 541.1696 543.1467 568.0932 570.1851 588.2028 599.3417 619.2383 647.2154 649.9970 665.9847 675.2422 842.4792 941.4568 971.3705 1075.5580 1163.6184 1463.7250 1568.8232 1651.9670 1655.8056 1783.9240 1886.6416 1902.8997 2125.1812 2211.1992

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
831.4856	831.4729	15.3673	74	80	(R)(H)
987.5949	987.5549	40.5074	358	366	(K)(A)
1466.8225	1466.8807	-39.7186	144	156	(R)(F)
1906.0066	1906.0148	-4.3010	484	500	(R)(-)

The matched peptides cover 9% (45/500 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/30 matches (13%). 45170.4 Da, pI = 6.17. Acc. # P22777. MOUSE. PLASMINOGEN ACTIVATOR INHIBITOR-1 PRECURSOR (PAI-1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1463.7250 1463.7494 -16.7245 112 124 (K)(D)
1466.8225 1466.7538 46.8058 77 88 (K)TRRQIQDAMGFK(V) 1Met-ox
1651.9670 1651.9244 25.8237 89 103 (K)(E)
2125.1812 2125.0777 48.6939 349 369 (R)(M)

26 unmatched masses: 525.1511 541.1696 543.1467 568.0932 570.1851 588.2028 599.3417 619.2383 647.2154 649.9970 665.9847 675.2422 831.4856 842.4792 941.4568 971.3705 987.5949 1075.5580 1163.6184 1568.8232 1655.8056 1783.9240 1886.6416 1902.8997 1906.0066 2211.1992

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1463.7250	1463.7494	-16.7245	112	124	(K)(D)
1466.8225	1466.7538	46.8058	77	88	(K)TRRQIQDAMGFK(V)	1Met-ox
1651.9670	1651.9244	25.8237	89	103	(K)(E)
2125.1812	2125.0777	48.6939	349	369	(R)(M)

The matched peptides cover 15% (61/402 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):