MS-Fit Search Results

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Sample ID (comment): HSG049
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 190 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
5

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
21

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.3e+004	10/21 (47%)	33298.9 / 5.11	HUMAN	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS C1/C2 (HNRNP C1 AND HNRNP C2).
2	47.1	4/21 (19%)	13173.9 / 11.38	HUMAN	60S RIBOSOMAL PROTEIN L34.
3	43	4/21 (19%)	25157.7 / 5.38	MOUSE	NATURAL KILLER CELL SURFACE PROTEIN P1-34 (NKR-P1 34).
4	40.9	5/21 (23%)	45465.1 / 5.51	MOUSE	TNF RECEPTOR ASSOCIATED FACTOR 1 (TRAF1).
4	40.6	4/21 (19%)	36978.2 / 6.20	HUMAN	UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR, GPI-ANCHORED FORM PRECURSOR (U-PAR) (MONOCYTE ACTIVATION ANTIGEN MO3) (CD87 ANTIGEN).

Detailed Results

1. 10/21 matches (47%). 33298.9 Da, pI = 5.11. Acc. # P07910. HUMAN. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS C1/C2 (HNRNP C1 AND HNRNP C2)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
731.2380	731.3000	-84.8381	131	135	(R)(M)
842.4559	842.4558	0.0887	43	50	(K)(G)
887.3621	887.4085	-52.3172	136	142	(R)(V)
903.3473	903.4035	-62.1753	136	142	(R)MYSYPAR(V)	1Met-ox
943.5294	943.5729	-46.1056	143	151	(R)(A)
1316.7511	1316.7942	-32.6982	18	29	(R)(K)
1316.7511	1316.7260	19.1300	158	168	(K)(G)
1329.6425	1329.6592	-12.5174	51	61	(K)(N)
1682.8908	1682.9151	-14.4640	74	89	(R)(V)
1698.8890	1698.9100	-12.3593	74	89	(R)MIAGQVLDINLAAEPK(V)	1Met-ox
1698.8890	1698.8523	21.6031	171	187	(K)(L)
2383.9623	2384.1251	-68.2746	224	244	(K)QAVEMKNDKSEEEQSSSSVKK(D)	1Met-ox

11 unmatched masses: 647.1980 1350.6606 1475.7074 1707.7617 1791.6982 1900.8450 2001.9388 2057.0062 2101.8787 2117.8799 2283.1956

The matched peptides cover 38% (117/303 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/21 matches (19%). 13173.9 Da, pI = 11.38. Acc. # P49207. HUMAN. 60S RIBOSOMAL PROTEIN L34..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
887.3621 887.3755 -15.1143 76 84 (R)(C)
903.3473 903.3704 -25.6314 76 84 (R)AYGGSMCAK(C) 1Met-ox
1682.8908 1683.0216 -77.7250 62 75 (K)(A)
1698.8890 1699.0165 -75.0249 62 75 (K)VLMRLSKTKKHVSR(A) 1Met-ox

17 unmatched masses: 647.1980 731.2380 842.4559 943.5294 1316.7511 1329.6425 1350.6606 1475.7074 1707.7617 1791.6982 1900.8450 2001.9388 2057.0062 2101.8787 2117.8799 2283.1956 2383.9623

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
887.3621	887.3755	-15.1143	76	84	(R)(C)
903.3473	903.3704	-25.6314	76	84	(R)AYGGSMCAK(C)	1Met-ox
1682.8908	1683.0216	-77.7250	62	75	(K)(A)
1698.8890	1699.0165	-75.0249	62	75	(K)VLMRLSKTKKHVSR(A)	1Met-ox

The matched peptides cover 19% (23/116 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/21 matches (19%). 25157.7 Da, pI = 5.38. Acc. # P27812. MOUSE. NATURAL KILLER CELL SURFACE PROTEIN P1-34 (NKR-P1 34)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1350.6606 1350.7581 -72.1556 34 43 (R)CPRWHRLALK(F) 1Cys-am
1682.8908 1682.8423 28.7783 1 15 (-)(I)
1698.8890 1698.8373 30.4760 1 15 (-)MDSTTLVYADLNLAR(I) 1Met-ox
1900.8450 1900.8500 -2.5864 208 223 (R)(-)

17 unmatched masses: 647.1980 731.2380 842.4559 887.3621 903.3473 943.5294 1316.7511 1329.6425 1475.7074 1707.7617 1791.6982 2001.9388 2057.0062 2101.8787 2117.8799 2283.1956 2383.9623

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1350.6606	1350.7581	-72.1556	34	43	(R)CPRWHRLALK(F)	1Cys-am
1682.8908	1682.8423	28.7783	1	15	(-)(I)
1698.8890	1698.8373	30.4760	1	15	(-)MDSTTLVYADLNLAR(I)	1Met-ox
1900.8450	1900.8500	-2.5864	208	223	(R)(-)

The matched peptides cover 17% (40/223 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/21 matches (23%). 45465.1 Da, pI = 5.51. Acc. # P39428. MOUSE. TNF RECEPTOR ASSOCIATED FACTOR 1 (TRAF1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
943.5294 943.5464 -18.0325 179 186 (K)(L)
1329.6425 1329.7717 -97.1379 313 323 (K)(G)
1350.6606 1350.6840 -17.2923 338 348 (K)(E)
1707.7617 1707.9216 -93.6185 298 312 (K)LCLRLYLNGDGSGKK(T) 1Cys-am
2383.9623 2384.0725 -46.2031 70 91 (K)VHSDVAEAEIMCPFAGVGCSFK(G) 1Met-ox 1Cys-am

16 unmatched masses: 647.1980 731.2380 842.4559 887.3621 903.3473 1316.7511 1475.7074 1682.8908 1698.8890 1791.6982 1900.8450 2001.9388 2057.0062 2101.8787 2117.8799 2283.1956

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
943.5294	943.5464	-18.0325	179	186	(K)(L)
1329.6425	1329.7717	-97.1379	313	323	(K)(G)
1350.6606	1350.6840	-17.2923	338	348	(K)(E)
1707.7617	1707.9216	-93.6185	298	312	(K)LCLRLYLNGDGSGKK(T)	1Cys-am
2383.9623	2384.0725	-46.2031	70	91	(K)VHSDVAEAEIMCPFAGVGCSFK(G)	1Met-ox 1Cys-am

The matched peptides cover 16% (67/409 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/21 matches (19%). 36978.2 Da, pI = 6.20. Acc. # Q03405. HUMAN. UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR, GPI-ANCHORED FORM PRECURSOR (U-PAR) (MONOCYTE ACTIVATION ANTIGEN MO3) (CD87 ANTIGEN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
731.2380 731.2857 -65.1652 215 220 (R)(G)
1329.6425 1329.5172 94.2529 25 35 (R)CMQCKTNGDCR(V) 1Cys-am
1682.8908 1682.7994 54.2786 239 254 (R)(N)
1698.8890 1698.7944 55.7362 239 254 (R)GPMNQCLVATGTHEPK(N) 1Met-ox

17 unmatched masses: 647.1980 842.4559 887.3621 903.3473 943.5294 1316.7511 1350.6606 1475.7074 1707.7617 1791.6982 1900.8450 2001.9388 2057.0062 2101.8787 2117.8799 2283.1956 2383.9623

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
731.2380	731.2857	-65.1652	215	220	(R)(G)
1329.6425	1329.5172	94.2529	25	35	(R)CMQCKTNGDCR(V)	1Cys-am
1682.8908	1682.7994	54.2786	239	254	(R)(N)
1698.8890	1698.7944	55.7362	239	254	(R)GPMNQCLVATGTHEPK(N)	1Met-ox

The matched peptides cover 9% (33/335 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):