MS-Fit Search Results

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Sample ID (comment): HSG050
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 654 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
5

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
25

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.07e+004	13/25 (52%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
2	1.16e+003	8/25 (32%)	26923.9 / 5.02	HUMAN	CHLORIDE INTRACELLULAR CHANNEL PROTEIN 1 (NUCLEAR CHLORIDE ION CHANNEL 27) (P64 CLCP).
3	176	6/25 (24%)	43135.8 / 6.28	HUMAN	EXTRACELLULAR SIGNAL-REGULATED KINASE 1 (EC 2.7.1.-) (ERK1) (INSULIN- STIMULATED MAP2 KINASE) (MAP KINASE 1) (MAPK 1) (P44-ERK1) (ERT2) (P44-MAPK) (MICROTUBULE-ASSOCIATED PROTEIN-2 KINASE).
4	91.9	7/25 (28%)	80645.9 / 6.70	MOUSE	PROTEIN-TYROSINE PHOSPHATASE EPSILON PRECURSOR (EC 3.1.3.48) (R-PTP- EPSILON).
4	91.6	6/25 (24%)	37374.9 / 5.85	HUMAN	FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN).

Detailed Results

1. 13/25 matches (52%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1092.5212	1092.5034	16.3190	602	615	(R)(G)
1179.5970	1179.6010	-3.3548	376	385	(K)(H)
1277.7055	1277.6337	56.1585	246	256	(K)(N)
1277.7055	1277.7105	-3.9475	472	482	(K)(T)
1383.6930	1383.6909	1.5784	185	196	(K)(V)
1475.7672	1475.7858	-12.6394	199	210	(R)(W)
1475.7672	1475.7494	12.0159	211	222	(K)(T)
1487.7655	1487.8043	-26.0465	239	250	(R)(L)
1638.8735	1638.8604	8.0105	185	198	(K)(F)
1657.8311	1657.7934	22.7202	12	28	(R)(R)
1716.8667	1716.8517	8.7830	417	431	(K)(G)
1844.9701	1844.9466	12.7181	416	431	(K)(G)
1994.0079	1993.9772	15.4191	223	238	(R)(R)
2150.0990	2150.0783	9.6058	223	239	(R)(R)
2384.0084	2383.9524	23.5132	518	548	(R)(G)

12 unmatched masses: 957.4505 1037.5379 1090.5330 1307.6717 1434.7795 1458.7463 1493.7572 1707.7823 2211.1551 2717.1745 2872.4219 2992.4775

The matched peptides cover 26% (171/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/25 matches (32%). 26923.9 Da, pI = 5.02. Acc. # O00299. HUMAN. CHLORIDE INTRACELLULAR CHANNEL PROTEIN 1 (NUCLEAR CHLORIDE ION CHANNEL 27) (P64 CLCP)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
957.4505 957.4794 -30.2278 209 216 (R)(E)
1037.5379 1037.5420 -3.9726 196 204 (R)(G)
1092.5212 1092.5260 -4.4128 21 29 (K)IGNCPFSQR(L) 1Cys-am
1458.7463 1458.7480 -1.1664 1 13 (-)AEEQPQVELFVK(A) Acet N
1844.9701 1844.9758 -3.0953 96 113 (K)(F)
2150.0990 2150.0440 25.5833 1 20 (-)MAEEQPQVELFVKAGSDGAK(I) 1Met-ox
2872.4219 2872.5123 -31.4623 193 216 (K)(E)
2992.4775 2992.3911 28.8700 139 165 (K)(K)

17 unmatched masses: 1090.5330 1179.5970 1277.7055 1307.6717 1383.6930 1434.7795 1475.7672 1487.7655 1493.7572 1638.8735 1657.8311 1707.7823 1716.8667 1994.0079 2211.1551 2384.0084 2717.1745

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
957.4505	957.4794	-30.2278	209	216	(R)(E)
1037.5379	1037.5420	-3.9726	196	204	(R)(G)
1092.5212	1092.5260	-4.4128	21	29	(K)IGNCPFSQR(L)	1Cys-am
1458.7463	1458.7480	-1.1664	1	13	(-)AEEQPQVELFVK(A)	Acet N
1844.9701	1844.9758	-3.0953	96	113	(K)(F)
2150.0990	2150.0440	25.5833	1	20	(-)MAEEQPQVELFVKAGSDGAK(I)	1Met-ox
2872.4219	2872.5123	-31.4623	193	216	(K)(E)
2992.4775	2992.3911	28.8700	139	165	(K)(K)

The matched peptides cover 40% (98/241 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/25 matches (24%). 43135.8 Da, pI = 6.28. Acc. # P27361. HUMAN. EXTRACELLULAR SIGNAL-REGULATED KINASE 1 (EC 2.7.1.-) (ERK1) (INSULIN- STIMULATED MAP2 KINASE) (MAP KINASE 1) (MAPK 1) (P44-ERK1) (ERT2) (P44-MAPK) (MICROTUBULE-ASSOCIATED PROTEIN-2 KINASE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
957.4505 957.5046 -56.4954 371 379 (R)(-)
1434.7795 1434.8181 -26.9046 97 108 (R)(A)
1707.7823 1707.8277 -26.6084 72 84 (K)KISPFEHQTYCQR(T) 1Cys-am
1844.9701 1844.8992 38.4303 117 131 (R)(L)
2717.1745 2717.4159 -88.8233 225 248 (K)SIDIWSVGCILAEMLSNRPIFPGK(H) 1Cys-am
2872.4219 2872.5620 -48.7468 72 94 (K)(F)

19 unmatched masses: 1037.5379 1090.5330 1092.5212 1179.5970 1277.7055 1307.6717 1383.6930 1458.7463 1475.7672 1487.7655 1493.7572 1638.8735 1657.8311 1716.8667 1994.0079 2150.0990 2211.1551 2384.0084 2992.4775

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
957.4505	957.5046	-56.4954	371	379	(R)(-)
1434.7795	1434.8181	-26.9046	97	108	(R)(A)
1707.7823	1707.8277	-26.6084	72	84	(K)KISPFEHQTYCQR(T)	1Cys-am
1844.9701	1844.8992	38.4303	117	131	(R)(L)
2717.1745	2717.4159	-88.8233	225	248	(K)SIDIWSVGCILAEMLSNRPIFPGK(H)	1Cys-am
2872.4219	2872.5620	-48.7468	72	94	(K)(F)

The matched peptides cover 21% (82/379 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/25 matches (28%). 80645.9 Da, pI = 6.70. Acc. # P49446. MOUSE. PROTEIN-TYROSINE PHOSPHATASE EPSILON PRECURSOR (EC 3.1.3.48) (R-PTP- EPSILON)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1434.7795 1434.8109 -21.8792 567 578 (R)(Q)
1434.7795 1434.7957 -11.2465 636 648 (R)(V)
1458.7463 1458.6874 40.4252 276 287 (K)FCIHPQLPDSCK(A) 1Cys-am
1475.7672 1475.8256 -39.5774 226 238 (R)(K)
1475.7672 1475.7099 38.8100 440 452 (K)(K)
1487.7655 1487.8658 -67.4051 72 84 (R)(M)
1657.8311 1657.8194 7.0242 275 287 (R)KFCIHPQLPDSCK(A) 2Cys-am
2384.0084 2384.1531 -60.6853 666 683 (R)LQRPHMVQTLEQYEFCYK(V) 1Cys-am
2717.1745 2717.4165 -89.0632 291 314 (R)(F)

18 unmatched masses: 957.4505 1037.5379 1090.5330 1092.5212 1179.5970 1277.7055 1307.6717 1383.6930 1493.7572 1638.8735 1707.7823 1716.8667 1844.9701 1994.0079 2150.0990 2211.1551 2872.4219 2992.4775

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1434.7795	1434.8109	-21.8792	567	578	(R)(Q)
1434.7795	1434.7957	-11.2465	636	648	(R)(V)
1458.7463	1458.6874	40.4252	276	287	(K)FCIHPQLPDSCK(A)	1Cys-am
1475.7672	1475.8256	-39.5774	226	238	(R)(K)
1475.7672	1475.7099	38.8100	440	452	(K)(K)
1487.7655	1487.8658	-67.4051	72	84	(R)(M)
1657.8311	1657.8194	7.0242	275	287	(R)KFCIHPQLPDSCK(A)	2Cys-am
2384.0084	2384.1531	-60.6853	666	683	(R)LQRPHMVQTLEQYEFCYK(V)	1Cys-am
2717.1745	2717.4165	-89.0632	291	314	(R)(F)

The matched peptides cover 17% (119/699 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/25 matches (24%). 37374.9 Da, pI = 5.85. Acc. # P19883. HUMAN. FOLLISTATIN 1 AND 2 PRECURSOR (FS) (ACTIVIN-BINDING PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1383.6930 1383.5594 96.5559 267 278 (R)CSLCDELCPDSK(S) 1Cys-am
1475.7672 1475.7130 36.6724 152 163 (K)(C)
1638.8735 1638.8638 5.9536 39 52 (K)(E)
2211.1551 2211.0143 63.7155 93 111 (K)ETCENVDCGPGKKCRMNKK(N) 1Cys-am
2717.1745 2717.2809 -39.1602 39 60 (K)NGRCQVLYKTELSKEECCSTGR(L) 3Cys-am
2872.4219 2872.4371 -5.2731 230 255 (R)SIGLAYEGKCIKAKSCEDIQCTGGKK(C) 2Cys-am

19 unmatched masses: 957.4505 1037.5379 1090.5330 1092.5212 1179.5970 1277.7055 1307.6717 1434.7795 1458.7463 1487.7655 1493.7572 1657.8311 1707.7823 1716.8667 1844.9701 1994.0079 2150.0990 2384.0084 2992.4775

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1383.6930	1383.5594	96.5559	267	278	(R)CSLCDELCPDSK(S)	1Cys-am
1475.7672	1475.7130	36.6724	152	163	(K)(C)
1638.8735	1638.8638	5.9536	39	52	(K)(E)
2211.1551	2211.0143	63.7155	93	111	(K)ETCENVDCGPGKKCRMNKK(N)	1Cys-am
2717.1745	2717.2809	-39.1602	39	60	(K)NGRCQVLYKTELSKEECCSTGR(L)	3Cys-am
2872.4219	2872.4371	-5.2731	230	255	(R)SIGLAYEGKCIKAKSCEDIQCTGGKK(C)	2Cys-am

The matched peptides cover 26% (91/338 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):