MS-Fit Search Results

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Sample ID (comment): HSG051
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 581 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
37

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.25e+004	12/37 (32%)	47154.7 / 4.94	HUMAN	GAMMA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NEURAL ENOLASE) (NSE).
2	1.7e+004	10/37 (27%)	47165.7 / 4.99	MOUSE	GAMMA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NEURAL ENOLASE) (NSE).
3	91.7	4/37 (10%)	64067.0 / 5.93	HUMAN	GTP-BINDING PROTEIN ARD-1.
4	85.3	7/37 (18%)	28680.8 / 10.85	HUMAN	40S RIBOSOMAL PROTEIN S6 (PHOSPHOPROTEIN NP33).
5	84.3	8/37 (21%)	71647.8 / 9.01	MOUSE	INTERFERON-ACTIVATABLE PROTEIN 204 (IFI-204).

Detailed Results

1. 12/37 matches (32%). 47154.7 Da, pI = 4.94. Acc. # P09104. HUMAN. GAMMA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NEURAL ENOLASE) (NSE)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
790.4890	790.4463	54.0386	126	131	(R)(H)
848.4708	848.4167	63.6555	422	428	(R)(N)
1019.5750	1019.5382	36.0774	335	342	(K)ACNCLLLK(V)	2Cys-am
1160.6099	1160.5435	57.1879	412	421	(R)(F)
1358.8006	1358.6969	76.3236	422	433	(R)(-)
1583.9129	1583.7753	86.9289	358	371	(K)(S)
1599.8927	1599.7702	76.5906	358	371	(K)LAQENGWGVMVSHR(S)	1Met-ox
1631.9295	1631.8427	53.2245	343	357	(K)VNQIGSVTEAIQACK(L)	1Cys-am
1805.0553	1804.9445	61.3955	32	49	(R)(D)
1840.9890	1840.8717	63.7293	253	268	(R)(Y)
1859.0323	1858.9339	52.9089	269	284	(R)(D)
1971.1135	1970.9720	71.8205	162	178	(K)LAMQEFMILPVGAESFR(D)	2Met-ox

25 unmatched masses: 601.3023 633.2436 644.3327 647.2542 650.1677 656.1157 662.4052 665.3300 683.3396 688.3405 691.2983 842.5541 882.6074 996.5834 1036.5880 1045.6211 1117.6746 1535.9848 1570.9027 1586.8878 1665.0447 1877.2117 1941.0637 2068.3076 2211.2659

The matched peptides cover 30% (131/433 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/37 matches (27%). 47165.7 Da, pI = 4.99. Acc. # P17183. MOUSE. GAMMA ENOLASE (EC 4.2.1.11) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE) (NEURAL ENOLASE) (NSE)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
848.4708 848.4167 63.6555 422 428 (R)(N)
1019.5750 1019.5382 36.0774 335 342 (K)ACNCLLLK(V) 2Cys-am
1160.6099 1160.5435 57.1879 412 421 (R)(F)
1358.8006 1358.6969 76.3236 422 433 (R)(-)
1583.9129 1583.7753 86.9289 358 371 (K)(S)
1599.8927 1599.7702 76.5906 358 371 (K)LAQENGWGVMVSHR(S) 1Met-ox
1631.9295 1631.8427 53.2245 343 357 (K)VNQIGSVTEAIQACK(L) 1Cys-am
1805.0553 1804.9445 61.3955 32 49 (R)(D)
1859.0323 1858.9339 52.9089 269 284 (R)(N)
1971.1135 1970.9720 71.8205 162 178 (K)LAMQEFMILPVGAESFR(D) 2Met-ox

27 unmatched masses: 601.3023 633.2436 644.3327 647.2542 650.1677 656.1157 662.4052 665.3300 683.3396 688.3405 691.2983 790.4890 842.5541 882.6074 996.5834 1036.5880 1045.6211 1117.6746 1535.9848 1570.9027 1586.8878 1665.0447 1840.9890 1877.2117 1941.0637 2068.3076 2211.2659

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
848.4708	848.4167	63.6555	422	428	(R)(N)
1019.5750	1019.5382	36.0774	335	342	(K)ACNCLLLK(V)	2Cys-am
1160.6099	1160.5435	57.1879	412	421	(R)(F)
1358.8006	1358.6969	76.3236	422	433	(R)(-)
1583.9129	1583.7753	86.9289	358	371	(K)(S)
1599.8927	1599.7702	76.5906	358	371	(K)LAQENGWGVMVSHR(S)	1Met-ox
1631.9295	1631.8427	53.2245	343	357	(K)VNQIGSVTEAIQACK(L)	1Cys-am
1805.0553	1804.9445	61.3955	32	49	(R)(D)
1859.0323	1858.9339	52.9089	269	284	(R)(N)
1971.1135	1970.9720	71.8205	162	178	(K)LAMQEFMILPVGAESFR(D)	2Met-ox

The matched peptides cover 25% (109/433 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/37 matches (10%). 64067.0 Da, pI = 5.93. Acc. # P36406. HUMAN. GTP-BINDING PROTEIN ARD-1..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1117.6746 1117.6621 11.1841 505 514 (R)(Q)
1583.9129 1583.7852 80.6801 302 315 (R)(E)
1599.8927 1599.7801 70.4042 302 315 (R)QEEMALSVVDAHVR(E) 1Met-ox
1599.8927 1599.7477 90.6356 550 563 (R)SGMGLYEGLDWLSR(Q) 1Met-ox
1840.9890 1840.8461 77.6483 28 44 (K)(V)
1840.9890 1840.9227 36.0296 302 317 (R)(L)

33 unmatched masses: 601.3023 633.2436 644.3327 647.2542 650.1677 656.1157 662.4052 665.3300 683.3396 688.3405 691.2983 790.4890 842.5541 848.4708 882.6074 996.5834 1019.5750 1036.5880 1045.6211 1160.6099 1358.8006 1535.9848 1570.9027 1586.8878 1631.9295 1665.0447 1805.0553 1859.0323 1877.2117 1941.0637 1971.1135 2068.3076 2211.2659

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1117.6746	1117.6621	11.1841	505	514	(R)(Q)
1583.9129	1583.7852	80.6801	302	315	(R)(E)
1599.8927	1599.7801	70.4042	302	315	(R)QEEMALSVVDAHVR(E)	1Met-ox
1599.8927	1599.7477	90.6356	550	563	(R)SGMGLYEGLDWLSR(Q)	1Met-ox
1840.9890	1840.8461	77.6483	28	44	(K)(V)
1840.9890	1840.9227	36.0296	302	317	(R)(L)

The matched peptides cover 9% (57/574 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/37 matches (18%). 28680.8 Da, pI = 10.85. Acc. # P10660. HUMAN. 40S RIBOSOMAL PROTEIN S6 (PHOSPHOPROTEIN NP33)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
633.2436 633.2844 -64.3503 150 154 (K)(Q)
665.3300 665.3106 29.0830 244 249 (K)(-)
1019.5750 1019.5672 7.6408 216 223 (K)(R)
1019.5750 1019.5672 7.6408 217 224 (R)(Q)
1840.9890 1841.0570 -36.9250 99 115 (R)GCIVDANLSVLNLVIVK(K) 1Cys-am
1859.0323 1858.9683 34.4108 80 94 (K)(K)
1859.0323 1859.0139 9.8829 150 164 (K)(E)
1941.0637 1941.1081 -22.8975 73 88 (R)(T)
2211.2659 2211.0756 86.0757 32 51 (R)MATEVAADALGEEWKGYVVR(I) 1Met-ox

30 unmatched masses: 601.3023 644.3327 647.2542 650.1677 656.1157 662.4052 683.3396 688.3405 691.2983 790.4890 842.5541 848.4708 882.6074 996.5834 1036.5880 1045.6211 1117.6746 1160.6099 1358.8006 1535.9848 1570.9027 1583.9129 1586.8878 1599.8927 1631.9295 1665.0447 1805.0553 1877.2117 1971.1135 2068.3076

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
633.2436	633.2844	-64.3503	150	154	(K)(Q)
665.3300	665.3106	29.0830	244	249	(K)(-)
1019.5750	1019.5672	7.6408	216	223	(K)(R)
1019.5750	1019.5672	7.6408	217	224	(R)(Q)
1840.9890	1841.0570	-36.9250	99	115	(R)GCIVDANLSVLNLVIVK(K)	1Cys-am
1859.0323	1858.9683	34.4108	80	94	(K)(K)
1859.0323	1859.0139	9.8829	150	164	(K)(E)
1941.0637	1941.1081	-22.8975	73	88	(R)(T)
2211.2659	2211.0756	86.0757	32	51	(R)MATEVAADALGEEWKGYVVR(I)	1Met-ox

The matched peptides cover 35% (88/249 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/37 matches (21%). 71647.8 Da, pI = 9.01. Acc. # P15092. MOUSE. INTERFERON-ACTIVATABLE PROTEIN 204 (IFI-204)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
601.3023 601.3310 -47.7196 545 550 (R)(I)
691.2983 691.3085 -14.7522 603 608 (R)CEPGSK(L) 1Cys-am
790.4890 790.4133 95.8097 349 354 (K)ICDIQK(G) 1Cys-am
1535.9848 1535.8368 96.3581 590 602 (K)(C)
1583.9129 1583.8222 57.2933 355 369 (K)(K)
1586.8878 1586.8153 45.6411 403 415 (K)(T)
1859.0323 1858.8910 75.9941 474 488 (K)RMFHATVATETEFFR(V) 1Met-ox
1971.1135 1971.0486 32.9497 416 432 (K)(I)

29 unmatched masses: 633.2436 644.3327 647.2542 650.1677 656.1157 662.4052 665.3300 683.3396 688.3405 842.5541 848.4708 882.6074 996.5834 1019.5750 1036.5880 1045.6211 1117.6746 1160.6099 1358.8006 1570.9027 1599.8927 1631.9295 1665.0447 1805.0553 1840.9890 1877.2117 1941.0637 2068.3076 2211.2659

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
601.3023	601.3310	-47.7196	545	550	(R)(I)
691.2983	691.3085	-14.7522	603	608	(R)CEPGSK(L)	1Cys-am
790.4890	790.4133	95.8097	349	354	(K)ICDIQK(G)	1Cys-am
1535.9848	1535.8368	96.3581	590	602	(K)(C)
1583.9129	1583.8222	57.2933	355	369	(K)(K)
1586.8878	1586.8153	45.6411	403	415	(K)(T)
1859.0323	1858.8910	75.9941	474	488	(K)RMFHATVATETEFFR(V)	1Met-ox
1971.1135	1971.0486	32.9497	416	432	(K)(I)

The matched peptides cover 14% (91/640 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):