MS-Fit Search Results

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Sample ID (comment): HSG052
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 167 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
50

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	557	6/50 (12%)	49541.8 / 7.26	HUMAN	ELONGATION FACTOR TU, MITOCHONDRIAL PRECURSOR (P43).
2	115	4/50 (8%)	25988.9 / 5.58	MOUSE	RAS-RELATED PROTEIN RAB-20.
3	61.5	5/50 (10%)	45836.3 / 9.77	HUMAN	MYELOID CELL NUCLEAR DIFFERENTIATION ANTIGEN.
4	60.5	9/50 (18%)	55249.8 / 9.43	HUMAN	ZINC FINGER PROTEIN 141.
5	46.8	5/50 (10%)	40418.5 / 9.06	HUMAN	WNT-2 PROTEIN PRECURSOR (IRP PROTEIN) (INT-1 RELATED PROTEIN).

Detailed Results

1. 6/50 matches (12%). 49541.8 Da, pI = 7.26. Acc. # P49411. HUMAN. ELONGATION FACTOR TU, MITOCHONDRIAL PRECURSOR (P43)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1185.5681	1185.6228	-46.1482	316	327	(R)(G)
1364.5708	1364.5970	-19.2380	92	102	(K)(A)
1492.6333	1492.6920	-39.3137	91	102	(K)(A)
1542.8173	1542.8532	-23.2643	239	252	(K)(D)
1673.8336	1673.8611	-16.4442	105	120	(R)(H)
1784.7122	1784.7815	-38.7923	121	135	(R)HYAHTDCPGHADYVK(N)	1Cys-am

44 unmatched masses: 621.1785 626.2625 634.2609 642.2148 646.1579 647.2083 651.2129 656.0568 661.2060 663.2427 683.2521 700.1902 713.3565 716.2288 721.2887 739.2390 753.2530 768.4664 772.2032 810.2720 842.4711 868.5201 882.5260 926.4032 1045.5077 1051.6777 1284.5351 1296.6226 1366.6276 1388.7270 1566.7224 1677.7879 1687.9573 1791.7109 1939.9232 1940.9316 1962.9174 2084.0504 2091.8907 2097.9446 2194.9254 2211.1003 2233.0327 2384.9691

The matched peptides cover 15% (69/452 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/50 matches (8%). 25988.9 Da, pI = 5.58. Acc. # P35295. MOUSE. RAS-RELATED PROTEIN RAB-20..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1296.6226 1296.6734 -39.2310 19 28 (K)(R)
1366.6276 1366.6755 -35.0919 48 59 (R)(E)
1566.7224 1566.6957 17.0046 209 222 (R)AEESDQTVDIASCK(T) 1Cys-am
2211.1003 2211.1120 -5.2931 95 114 (R)FLGLTETANNDCLFAIVGNK(V) 1Cys-am

46 unmatched masses: 621.1785 626.2625 634.2609 642.2148 646.1579 647.2083 651.2129 656.0568 661.2060 663.2427 683.2521 700.1902 713.3565 716.2288 721.2887 739.2390 753.2530 768.4664 772.2032 810.2720 842.4711 868.5201 882.5260 926.4032 1045.5077 1051.6777 1185.5681 1284.5351 1364.5708 1388.7270 1492.6333 1542.8173 1673.8336 1677.7879 1687.9573 1784.7122 1791.7109 1939.9232 1940.9316 1962.9174 2084.0504 2091.8907 2097.9446 2194.9254 2233.0327 2384.9691

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1296.6226	1296.6734	-39.2310	19	28	(K)(R)
1366.6276	1366.6755	-35.0919	48	59	(R)(E)
1566.7224	1566.6957	17.0046	209	222	(R)AEESDQTVDIASCK(T)	1Cys-am
2211.1003	2211.1120	-5.2931	95	114	(R)FLGLTETANNDCLFAIVGNK(V)	1Cys-am

The matched peptides cover 24% (56/233 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/50 matches (10%). 45836.3 Da, pI = 9.77. Acc. # P41218. HUMAN. MYELOID CELL NUCLEAR DIFFERENTIATION ANTIGEN..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
842.4711 842.4848 -16.3281 77 83 (K)(K)
868.5201 868.5369 -19.2574 128 135 (R)(R)
1673.8336 1673.8395 -3.5409 315 329 (K)(K)
1939.9232 1939.8786 23.0067 282 298 (K)(I)
2084.0504 2083.9759 35.7398 225 243 (K)(T)

45 unmatched masses: 621.1785 626.2625 634.2609 642.2148 646.1579 647.2083 651.2129 656.0568 661.2060 663.2427 683.2521 700.1902 713.3565 716.2288 721.2887 739.2390 753.2530 768.4664 772.2032 810.2720 882.5260 926.4032 1045.5077 1051.6777 1185.5681 1284.5351 1296.6226 1364.5708 1366.6276 1388.7270 1492.6333 1542.8173 1566.7224 1677.7879 1687.9573 1784.7122 1791.7109 1940.9316 1962.9174 2091.8907 2097.9446 2194.9254 2211.1003 2233.0327 2384.9691

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
842.4711	842.4848	-16.3281	77	83	(K)(K)
868.5201	868.5369	-19.2574	128	135	(R)(R)
1673.8336	1673.8395	-3.5409	315	329	(K)(K)
1939.9232	1939.8786	23.0067	282	298	(K)(I)
2084.0504	2083.9759	35.7398	225	243	(K)(T)

The matched peptides cover 16% (66/407 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/50 matches (18%). 55249.8 Da, pI = 9.43. Acc. # Q15928. HUMAN. ZINC FINGER PROTEIN 141..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
739.2390 739.2755 -49.3938 257 262 (K)CEECGK(A) 1Cys-am
739.2390 739.2755 -49.3938 313 318 (K)CEECGK(A) 1Cys-am
739.2390 739.2755 -49.3938 397 402 (K)CEECGK(A) 1Cys-am
768.4664 768.4368 38.4685 354 359 (K)(V)
926.4032 926.4406 -40.3794 175 182 (K)(F)
1364.5708 1364.6269 -41.1193 20 30 (K)(D)
1542.8173 1542.7633 34.9702 106 118 (K)CGHDNLQLRKGCK(S) 1Cys-am
1939.9232 1939.9635 -20.7440 453 468 (K)(H)
1940.9316 1940.9799 -24.8874 275 290 (K)RIHAGEKPITCEECRK(I) 1Cys-am
1962.9174 1962.9642 -23.8715 103 118 (R)YEKCGHDNLQLRKGCK(S) 1Cys-am
2211.1003 2211.0327 30.5705 332 349 (R)IHTGEKPYTCEECGKAFR(Q) 2Cys-am
2211.1003 2211.1055 -2.3421 425 442 (K)CKECDKAFKQFSLLSQHK(K) 1Cys-am

41 unmatched masses: 621.1785 626.2625 634.2609 642.2148 646.1579 647.2083 651.2129 656.0568 661.2060 663.2427 683.2521 700.1902 713.3565 716.2288 721.2887 753.2530 772.2032 810.2720 842.4711 868.5201 882.5260 1045.5077 1051.6777 1185.5681 1284.5351 1296.6226 1366.6276 1388.7270 1492.6333 1566.7224 1673.8336 1677.7879 1687.9573 1784.7122 1791.7109 2084.0504 2091.8907 2097.9446 2194.9254 2233.0327 2384.9691

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
739.2390	739.2755	-49.3938	257	262	(K)CEECGK(A)	1Cys-am
739.2390	739.2755	-49.3938	313	318	(K)CEECGK(A)	1Cys-am
739.2390	739.2755	-49.3938	397	402	(K)CEECGK(A)	1Cys-am
768.4664	768.4368	38.4685	354	359	(K)(V)
926.4032	926.4406	-40.3794	175	182	(K)(F)
1364.5708	1364.6269	-41.1193	20	30	(K)(D)
1542.8173	1542.7633	34.9702	106	118	(K)CGHDNLQLRKGCK(S)	1Cys-am
1939.9232	1939.9635	-20.7440	453	468	(K)(H)
1940.9316	1940.9799	-24.8874	275	290	(K)RIHAGEKPITCEECRK(I)	1Cys-am
1962.9174	1962.9642	-23.8715	103	118	(R)YEKCGHDNLQLRKGCK(S)	1Cys-am
2211.1003	2211.0327	30.5705	332	349	(R)IHTGEKPYTCEECGKAFR(Q)	2Cys-am
2211.1003	2211.1055	-2.3421	425	442	(K)CKECDKAFKQFSLLSQHK(K)	1Cys-am

The matched peptides cover 26% (127/474 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/50 matches (10%). 40418.5 Da, pI = 9.06. Acc. # P09544. HUMAN. WNT-2 PROTEIN PRECURSOR (IRP PROTEIN) (INT-1 RELATED PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
739.2390 739.2755 -49.3938 134 140 (K)(K)
1284.5351 1284.5869 -40.3335 219 228 (R)TCWLAMADFR(K) 1Cys-am
1962.9174 1962.9779 -30.8688 281 299 (R)(G)
2233.0327 2233.0687 -16.0958 219 236 (R)(K)
2384.9691 2385.0725 -43.3394 208 228 (K)CHGVSGSCTLRTCWLAMADFR(K) 1Cys-am

45 unmatched masses: 621.1785 626.2625 634.2609 642.2148 646.1579 647.2083 651.2129 656.0568 661.2060 663.2427 683.2521 700.1902 713.3565 716.2288 721.2887 753.2530 768.4664 772.2032 810.2720 842.4711 868.5201 882.5260 926.4032 1045.5077 1051.6777 1185.5681 1296.6226 1364.5708 1366.6276 1388.7270 1492.6333 1542.8173 1566.7224 1673.8336 1677.7879 1687.9573 1784.7122 1791.7109 1939.9232 1940.9316 2084.0504 2091.8907 2097.9446 2194.9254 2211.1003

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
739.2390	739.2755	-49.3938	134	140	(K)(K)
1284.5351	1284.5869	-40.3335	219	228	(R)TCWLAMADFR(K)	1Cys-am
1962.9174	1962.9779	-30.8688	281	299	(R)(G)
2233.0327	2233.0687	-16.0958	219	236	(R)(K)
2384.9691	2385.0725	-43.3394	208	228	(K)CHGVSGSCTLRTCWLAMADFR(K)	1Cys-am

The matched peptides cover 15% (55/360 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):