MS-Fit Search Results

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Sample ID (comment): HSG053
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 768 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
52

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.15e+006	16/52 (30%)	26923.9 / 5.02	HUMAN	CHLORIDE INTRACELLULAR CHANNEL PROTEIN 1 (NUCLEAR CHLORIDE ION CHANNEL 27) (P64 CLCP).
2	1.76e+003	10/52 (19%)	46883.8 / 7.77	HUMAN	VASCULAR ENDOTHELIAL GROWTH FACTOR C PRECURSOR (VEGF-C) (VASCULAR ENDOTHELIAL GROWTH FACTOR RELATED PROTEIN) (VRP) (FLT4 LIGAND).
3	816	6/52 (11%)	80547.2 / 4.67	HUMAN	NIL-2-A ZINC FINGER PROTEIN (NEGATIVE REGULATOR OF IL2) (TRANSCRIPTION FACTOR 8).
4	310	9/52 (17%)	78242.3 / 8.97	HUMAN	ZINC FINGER PROTEIN 45 (BRC1744).
5	301	8/52 (15%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).

Detailed Results

1. 16/52 matches (30%). 26923.9 Da, pI = 5.02. Acc. # O00299. HUMAN. CHLORIDE INTRACELLULAR CHANNEL PROTEIN 1 (NUCLEAR CHLORIDE ION CHANNEL 27) (P64 CLCP)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
957.4607	957.4794	-19.5205	209	216	(R)(E)
1037.5212	1037.5420	-20.0269	196	204	(R)(G)
1092.5077	1092.5260	-16.7833	21	29	(K)IGNCPFSQR(L)	1Cys-am
1109.6013	1109.6505	-44.3684	184	192	(K)LHIVQVVCK(K)	1Cys-am
1237.7194	1237.7455	-21.0847	184	193	(K)LHIVQVVCKK(Y)	1Cys-am
1328.6265	1328.6446	-13.6515	120	131	(K)(G)
1356.6994	1356.7064	-5.1864	194	204	(K)(G)
1458.7472	1458.7480	-0.5700	1	13	(-)AEEQPQVELFVK(A)	Acet N
1844.9742	1844.9758	-0.8752	96	113	(K)(F)
1947.0133	1946.9897	12.1098	167	183	(K)FLDGNELTLADCNLLPK(L)	1Cys-am
1975.0577	1975.0363	10.8405	80	95	(K)IEEFLEAVLCPPRYPK(L)	1Cys-am
2075.1158	2075.0847	14.9727	166	183	(R)KFLDGNELTLADCNLLPK(L)	1Cys-am
2233.0944	2233.1869	-41.3849	93	113	(R)(F)
2587.2035	2587.1398	24.6452	217	238	(R)EEFASTCPDDEEIELAYEQVAK(A)	1Cys-am
2992.4941	2992.3911	34.4164	139	165	(K)(K)
3120.5023	3120.4861	5.2140	139	166	(K)(F)

36 unmatched masses: 600.2763 602.1859 625.2169 633.2325 647.2245 651.1932 728.3183 752.2570 810.3475 842.4882 868.5033 1040.5202 1045.5356 1179.5977 1307.6191 1475.7686 1480.7482 1687.9705 1707.8081 1766.7681 1791.7269 1866.9937 1940.9647 2041.1599 2097.0430 2211.1410 2300.2700 2384.0216 2609.2073 2705.2176 2717.2297 2719.1539 2990.5352 2996.4344 3121.6323 3224.4023

The matched peptides cover 68% (164/241 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/52 matches (19%). 46883.8 Da, pI = 7.77. Acc. # P49767. HUMAN. VASCULAR ENDOTHELIAL GROWTH FACTOR C PRECURSOR (VEGF-C) (VASCULAR ENDOTHELIAL GROWTH FACTOR RELATED PROTEIN) (VRP) (FLT4 LIGAND)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
957.4607 957.4650 -4.4939 80 86 (K)MYKCQLR(K) 1Met-ox
1092.5077 1092.5260 -16.7833 323 332 (K)(E)
1480.7482 1480.7807 -21.9531 345 357 (K)(C)
1766.7681 1766.8435 -42.6801 88 102 (K)(T)
2233.0944 2233.1116 -7.6784 143 161 (K)EFGVATNTFFKPPCVSVYR(C) 1Cys-am
2705.2176 2705.1580 22.0254 323 344 (K)LFPSQCGANREFDENTCQCVCK(R) 3Cys-am
2717.2297 2717.2268 1.0878 346 368 (R)TCPRNQPLNPGKCACECTESPQK(C) 3Cys-am
2717.2297 2717.2883 -21.5589 350 372 (R)NQPLNPGKCACECTESPQKCLLK(G) 3Cys-am
2719.1539 2719.1849 -11.3910 323 345 (K)LFPSQCGANREFDENTCQCVCKR(T) 1Cys-am
2992.4941 2992.4755 6.2136 369 391 (K)CLLKGKKFHHQTCSCYRRPCTNR(Q) 3Cys-am
2996.4344 2996.3649 23.1804 24 54 (R)(D)

42 unmatched masses: 600.2763 602.1859 625.2169 633.2325 647.2245 651.1932 728.3183 752.2570 810.3475 842.4882 868.5033 1037.5212 1040.5202 1045.5356 1109.6013 1179.5977 1237.7194 1307.6191 1328.6265 1356.6994 1458.7472 1475.7686 1687.9705 1707.8081 1791.7269 1844.9742 1866.9937 1940.9647 1947.0133 1975.0577 2041.1599 2075.1158 2097.0430 2211.1410 2300.2700 2384.0216 2587.2035 2609.2073 2990.5352 3120.5023 3121.6323 3224.4023

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
957.4607	957.4650	-4.4939	80	86	(K)MYKCQLR(K)	1Met-ox
1092.5077	1092.5260	-16.7833	323	332	(K)(E)
1480.7482	1480.7807	-21.9531	345	357	(K)(C)
1766.7681	1766.8435	-42.6801	88	102	(K)(T)
2233.0944	2233.1116	-7.6784	143	161	(K)EFGVATNTFFKPPCVSVYR(C)	1Cys-am
2705.2176	2705.1580	22.0254	323	344	(K)LFPSQCGANREFDENTCQCVCK(R)	3Cys-am
2717.2297	2717.2268	1.0878	346	368	(R)TCPRNQPLNPGKCACECTESPQK(C)	3Cys-am
2717.2297	2717.2883	-21.5589	350	372	(R)NQPLNPGKCACECTESPQKCLLK(G)	3Cys-am
2719.1539	2719.1849	-11.3910	323	345	(K)LFPSQCGANREFDENTCQCVCKR(T)	1Cys-am
2992.4941	2992.4755	6.2136	369	391	(K)CLLKGKKFHHQTCSCYRRPCTNR(Q)	3Cys-am
2996.4344	2996.3649	23.1804	24	54	(R)(D)

The matched peptides cover 33% (141/419 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/52 matches (11%). 80547.2 Da, pI = 4.67. Acc. # P37275. HUMAN. NIL-2-A ZINC FINGER PROTEIN (NEGATIVE REGULATOR OF IL2) (TRANSCRIPTION FACTOR 8)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1307.6191 1307.5967 17.1323 720 731 (K)(T)
1475.7686 1475.6953 49.6766 564 575 (R)LHSGEKPYQCDK(C) 1Cys-am
2609.2073 2609.2348 -10.5382 506 526 (K)KMRKTENGMYACDLCDKIFQK(S) 1Met-ox 1Cys-am
2705.2176 2705.2515 -12.5382 269 293 (K)(M)
2990.5352 2990.4635 23.9890 326 352 (R)(Q)
2996.4344 2996.3327 33.9092 132 159 (K)SFEGGVNDSTCLLCDDCPGDINALPELK(H) 1Cys-am

46 unmatched masses: 600.2763 602.1859 625.2169 633.2325 647.2245 651.1932 728.3183 752.2570 810.3475 842.4882 868.5033 957.4607 1037.5212 1040.5202 1045.5356 1092.5077 1109.6013 1179.5977 1237.7194 1328.6265 1356.6994 1458.7472 1480.7482 1687.9705 1707.8081 1766.7681 1791.7269 1844.9742 1866.9937 1940.9647 1947.0133 1975.0577 2041.1599 2075.1158 2097.0430 2211.1410 2233.0944 2300.2700 2384.0216 2587.2035 2717.2297 2719.1539 2992.4941 3120.5023 3121.6323 3224.4023

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1307.6191	1307.5967	17.1323	720	731	(K)(T)
1475.7686	1475.6953	49.6766	564	575	(R)LHSGEKPYQCDK(C)	1Cys-am
2609.2073	2609.2348	-10.5382	506	526	(K)KMRKTENGMYACDLCDKIFQK(S)	1Met-ox 1Cys-am
2705.2176	2705.2515	-12.5382	269	293	(K)(M)
2990.5352	2990.4635	23.9890	326	352	(R)(Q)
2996.4344	2996.3327	33.9092	132	159	(K)SFEGGVNDSTCLLCDDCPGDINALPELK(H)	1Cys-am

The matched peptides cover 17% (125/735 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/52 matches (17%). 78242.3 Da, pI = 8.97. Acc. # Q02386. HUMAN. ZINC FINGER PROTEIN 45 (BRC1744)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
810.3475 810.3126 43.1260 306 311 (K)CEECGK(S) 2Cys-am
810.3475 810.3126 43.1260 362 367 (K)CEECGK(G) 2Cys-am
810.3475 810.3126 43.1260 390 395 (K)CEECGK(G) 2Cys-am
810.3475 810.3126 43.1260 446 451 (K)CEECGK(G) 2Cys-am
810.3475 810.3126 43.1260 614 619 (K)CEECGK(V) 2Cys-am
810.3475 810.3126 43.1260 642 647 (K)CEECGK(G) 2Cys-am
1707.8081 1707.7835 14.4160 437 451 (R)(G)
1707.8081 1707.7947 7.8382 465 479 (R)GHTGEKPYKCGTCGK(G) 2Cys-am
1707.8081 1707.7835 14.4160 633 647 (R)(G)
1791.7269 1791.8158 -49.6309 521 535 (R)VHTGEKPYQCAECGK(G) 2Cys-am
2211.1410 2211.0504 40.9348 241 259 (R)VPTGENPYKYEECGRNVGK(S) 1Cys-am
2211.1410 2211.0552 38.8053 502 520 (K)(V)
2233.0944 2233.0647 13.3389 302 318 (K)KPYKCEECGKSFSWRSR(L) 2Cys-am
2609.2073 2609.3013 -36.0230 424 445 (K)(C)
2705.2176 2705.3292 -41.2449 278 301 (K)(K)
2719.1539 2719.2833 -47.5737 597 619 (R)(V)
2990.5352 2990.4478 29.2511 480 504 (K)GFSRSSDLNVHCRIHTGEKPYKCER(C) 1Cys-am

43 unmatched masses: 600.2763 602.1859 625.2169 633.2325 647.2245 651.1932 728.3183 752.2570 842.4882 868.5033 957.4607 1037.5212 1040.5202 1045.5356 1092.5077 1109.6013 1179.5977 1237.7194 1307.6191 1328.6265 1356.6994 1458.7472 1475.7686 1480.7482 1687.9705 1766.7681 1844.9742 1866.9937 1940.9647 1947.0133 1975.0577 2041.1599 2075.1158 2097.0430 2300.2700 2384.0216 2587.2035 2717.2297 2992.4941 2996.4344 3120.5023 3121.6323 3224.4023

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
810.3475	810.3126	43.1260	306	311	(K)CEECGK(S)	2Cys-am
810.3475	810.3126	43.1260	362	367	(K)CEECGK(G)	2Cys-am
810.3475	810.3126	43.1260	390	395	(K)CEECGK(G)	2Cys-am
810.3475	810.3126	43.1260	446	451	(K)CEECGK(G)	2Cys-am
810.3475	810.3126	43.1260	614	619	(K)CEECGK(V)	2Cys-am
810.3475	810.3126	43.1260	642	647	(K)CEECGK(G)	2Cys-am
1707.8081	1707.7835	14.4160	437	451	(R)(G)
1707.8081	1707.7947	7.8382	465	479	(R)GHTGEKPYKCGTCGK(G)	2Cys-am
1707.8081	1707.7835	14.4160	633	647	(R)(G)
1791.7269	1791.8158	-49.6309	521	535	(R)VHTGEKPYQCAECGK(G)	2Cys-am
2211.1410	2211.0504	40.9348	241	259	(R)VPTGENPYKYEECGRNVGK(S)	1Cys-am
2211.1410	2211.0552	38.8053	502	520	(K)(V)
2233.0944	2233.0647	13.3389	302	318	(K)KPYKCEECGKSFSWRSR(L)	2Cys-am
2609.2073	2609.3013	-36.0230	424	445	(K)(C)
2705.2176	2705.3292	-41.2449	278	301	(K)(K)
2719.1539	2719.2833	-47.5737	597	619	(R)(V)
2990.5352	2990.4478	29.2511	480	504	(K)GFSRSSDLNVHCRIHTGEKPYKCER(C)	1Cys-am

The matched peptides cover 30% (209/682 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/52 matches (15%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1045.5356 1045.5754 -38.0644 177 184 (K)(S)
1092.5077 1092.5034 3.9483 602 615 (R)(G)
1179.5977 1179.6010 -2.8105 376 385 (K)(H)
1475.7686 1475.7858 -11.6697 199 210 (R)(W)
1475.7686 1475.7494 12.9856 211 222 (K)(T)
1687.9705 1687.9707 -0.0893 65 81 (R)(G)
1844.9742 1844.9466 14.9382 416 431 (K)(G)
2300.2700 2300.1985 31.0842 343 363 (R)(S)
2384.0216 2383.9524 29.0196 518 548 (R)(G)

44 unmatched masses: 600.2763 602.1859 625.2169 633.2325 647.2245 651.1932 728.3183 752.2570 810.3475 842.4882 868.5033 957.4607 1037.5212 1040.5202 1109.6013 1237.7194 1307.6191 1328.6265 1356.6994 1458.7472 1480.7482 1707.8081 1766.7681 1791.7269 1866.9937 1940.9647 1947.0133 1975.0577 2041.1599 2075.1158 2097.0430 2211.1410 2233.0944 2587.2035 2609.2073 2705.2176 2717.2297 2719.1539 2990.5352 2992.4941 2996.4344 3120.5023 3121.6323 3224.4023

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1045.5356	1045.5754	-38.0644	177	184	(K)(S)
1092.5077	1092.5034	3.9483	602	615	(R)(G)
1179.5977	1179.6010	-2.8105	376	385	(K)(H)
1475.7686	1475.7858	-11.6697	199	210	(R)(W)
1475.7686	1475.7494	12.9856	211	222	(K)(T)
1687.9705	1687.9707	-0.0893	65	81	(R)(G)
1844.9742	1844.9466	14.9382	416	431	(K)(G)
2300.2700	2300.1985	31.0842	343	363	(R)(S)
2384.0216	2383.9524	29.0196	518	548	(R)(G)

The matched peptides cover 21% (141/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):