MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG054
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 197 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
100.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
27

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	262	6/27 (22%)	49850.0 / 8.49	MOUSE	BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N- ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.102) (CORE 2 BRANCHING ENZYME) (CORE2-GLCNAC-TRANSFERASE) (C2GNT).
2	177	5/27 (18%)	35922.2 / 8.58	HUMAN	GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER (EC 1.2.1.12).
3	44.1	4/27 (14%)	46883.8 / 7.77	HUMAN	VASCULAR ENDOTHELIAL GROWTH FACTOR C PRECURSOR (VEGF-C) (VASCULAR ENDOTHELIAL GROWTH FACTOR RELATED PROTEIN) (VRP) (FLT4 LIGAND).
4	39	4/27 (14%)	77887.5 / 8.67	HUMAN	ZINC FINGER PROTEIN 7 (ZINC FINGER PROTEIN KOX4) (ZINC FINGER PROTEIN HF.16).
5	36.1	4/27 (14%)	28821.0 / 7.50	MOUSE	FOLATE RECEPTOR BETA PRECURSOR (FR-BETA) (FOLATE RECEPTOR 2) (FOLATE- BINDING PROTEIN 2).

Detailed Results

1. 6/27 matches (22%). 49850.0 Da, pI = 8.49. Acc. # Q09324. MOUSE. BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N- ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.102) (CORE 2 BRANCHING ENZYME) (CORE2-GLCNAC-TRANSFERASE) (C2GNT)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
882.5010	882.4144	98.2165	99	105	(R)DCASFIR(T)	1Cys-am
1544.7460	1544.8113	-42.3026	280	293	(K)(E)
1613.8281	1613.8071	12.9705	212	224	(K)YLINLCGMDFPIK(T)	1Met-ox 1Cys-am
1940.9075	1940.9151	-3.9401	142	156	(R)AIYMPQNFYCIHVDR(K)	1Cys-am
2211.0731	2211.1960	-55.5813	99	116	(R)DCASFIRTRKYIVEPLTK(E)	1Cys-am
2235.0375	2235.0215	7.1905	402	419	(K)FDMDVDPFAIQCLDEHLR(R)	1Cys-am

21 unmatched masses: 713.3434 715.2307 721.2396 725.3297 731.2348 768.4567 806.3109 825.0653 836.3176 842.4600 864.4139 868.4632 914.3312 996.5143 1045.5054 1051.6219 1566.6885 1620.7122 1763.7466 2041.0289 2233.1154

The matched peptides cover 18% (78/428 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/27 matches (18%). 35922.2 Da, pI = 8.58. Acc. # P04406. HUMAN. GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER (EC 1.2.1.12)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1544.7460 1544.8107 -41.8806 234 247 (R)VPTANVSVVDLTCR(L) 1Cys-am
1613.8281 1613.9015 -45.5031 66 79 (K)(D)
1763.7466 1763.8029 -31.9461 309 322 (K)(V)
2041.0289 2041.1082 -38.8744 66 83 (K)(I)
2211.0731 2211.2171 -65.1363 234 253 (R)VPTANVSVVDLTCRLEKPAK(Y) 1Cys-am

22 unmatched masses: 713.3434 715.2307 721.2396 725.3297 731.2348 768.4567 806.3109 825.0653 836.3176 842.4600 864.4139 868.4632 882.5010 914.3312 996.5143 1045.5054 1051.6219 1566.6885 1620.7122 1940.9075 2233.1154 2235.0375

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1544.7460	1544.8107	-41.8806	234	247	(R)VPTANVSVVDLTCR(L)	1Cys-am
1613.8281	1613.9015	-45.5031	66	79	(K)(D)
1763.7466	1763.8029	-31.9461	309	322	(K)(V)
2041.0289	2041.1082	-38.8744	66	83	(K)(I)
2211.0731	2211.2171	-65.1363	234	253	(R)VPTANVSVVDLTCRLEKPAK(Y)	1Cys-am

The matched peptides cover 15% (52/334 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/27 matches (14%). 46883.8 Da, pI = 7.77. Acc. # P49767. HUMAN. VASCULAR ENDOTHELIAL GROWTH FACTOR C PRECURSOR (VEGF-C) (VASCULAR ENDOTHELIAL GROWTH FACTOR RELATED PROTEIN) (VRP) (FLT4 LIGAND)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
806.3109 806.3653 -67.4889 129 134 (K)TQCMPR(E) 1Cys-am
882.5010 882.4184 93.6573 408 414 (R)(R)
1763.7466 1763.8321 -48.5052 121 134 (K)(E)
2233.1154 2233.1116 1.7201 143 161 (K)EFGVATNTFFKPPCVSVYR(C) 1Cys-am

23 unmatched masses: 713.3434 715.2307 721.2396 725.3297 731.2348 768.4567 825.0653 836.3176 842.4600 864.4139 868.4632 914.3312 996.5143 1045.5054 1051.6219 1544.7460 1566.6885 1613.8281 1620.7122 1940.9075 2041.0289 2211.0731 2235.0375

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
806.3109	806.3653	-67.4889	129	134	(K)TQCMPR(E)	1Cys-am
882.5010	882.4184	93.6573	408	414	(R)(R)
1763.7466	1763.8321	-48.5052	121	134	(K)(E)
2233.1154	2233.1116	1.7201	143	161	(K)EFGVATNTFFKPPCVSVYR(C)	1Cys-am

The matched peptides cover 9% (40/419 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/27 matches (14%). 77887.5 Da, pI = 8.67. Acc. # P17097. HUMAN. ZINC FINGER PROTEIN 7 (ZINC FINGER PROTEIN KOX4) (ZINC FINGER PROTEIN HF.16)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
725.3297 725.2598 96.2668 415 420 (K)CDECGK(A) 1Cys-am
725.3297 725.2598 96.2668 471 476 (K)CDECGK(G) 1Cys-am
1763.7466 1763.8209 -42.1368 655 669 (R)IHTGEKPYKCNDCGK(A) 1Cys-am
2211.0731 2211.0756 -1.1357 143 161 (K)VTGFTFQNNCLNEETVVPK(T) 1Cys-am
2233.1154 2232.9225 86.4096 240 257 (K)HTNNCHGEKPYECAECGK(V) 3Cys-am

23 unmatched masses: 713.3434 715.2307 721.2396 731.2348 768.4567 806.3109 825.0653 836.3176 842.4600 864.4139 868.4632 882.5010 914.3312 996.5143 1045.5054 1051.6219 1544.7460 1566.6885 1613.8281 1620.7122 1940.9075 2041.0289 2235.0375

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
725.3297	725.2598	96.2668	415	420	(K)CDECGK(A)	1Cys-am
725.3297	725.2598	96.2668	471	476	(K)CDECGK(G)	1Cys-am
1763.7466	1763.8209	-42.1368	655	669	(R)IHTGEKPYKCNDCGK(A)	1Cys-am
2211.0731	2211.0756	-1.1357	143	161	(K)VTGFTFQNNCLNEETVVPK(T)	1Cys-am
2233.1154	2232.9225	86.4096	240	257	(K)HTNNCHGEKPYECAECGK(V)	3Cys-am

The matched peptides cover 9% (64/686 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/27 matches (14%). 28821.0 Da, pI = 7.50. Acc. # Q05685. MOUSE. FOLATE RECEPTOR BETA PRECURSOR (FR-BETA) (FOLATE RECEPTOR 2) (FOLATE- BINDING PROTEIN 2)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
725.3297 725.3582 -39.3925 191 196 (K)(G)
842.4600 842.4194 48.1818 140 146 (R)(D)
2211.0731 2210.9851 39.8096 201 219 (R)(F)
2233.1154 2233.0454 31.3713 55 73 (K)KNACCSVNTSQELHKADSR(L) 2Cys-am

23 unmatched masses: 713.3434 715.2307 721.2396 731.2348 768.4567 806.3109 825.0653 836.3176 864.4139 868.4632 882.5010 914.3312 996.5143 1045.5054 1051.6219 1544.7460 1566.6885 1613.8281 1620.7122 1763.7466 1940.9075 2041.0289 2235.0375

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
725.3297	725.3582	-39.3925	191	196	(K)(G)
842.4600	842.4194	48.1818	140	146	(R)(D)
2211.0731	2210.9851	39.8096	201	219	(R)(F)
2233.1154	2233.0454	31.3713	55	73	(K)KNACCSVNTSQELHKADSR(L)	2Cys-am

The matched peptides cover 20% (51/251 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):