MS-Fit Search Results


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Sample ID (comment): HSG055
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 173 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |
Min. # Peptides
to Match
4
Peptide Mass
Tolerance (+/-)
75.000 ppm
Peptide Masses
are
monoisotopic
Digest
Used
Trypsin
Max. # Missed
Cleavages
4
Cysteines
Modified by
unmodified
Peptide
N terminus
Hydrogen (H)
Peptide
C terminus
Free Acid (O H)
Input #
Peptide Masses
31

Result Summary

RankMOWSE
Score
# (%)
Masses
Matched
Protein
MW (Da)/pI
SpeciesSwissProt.r36
Accession #
Protein Name
11.15e+0038/31 (25%) 28723.3 / 5.78HUMANINTERFERON GAMMA UP-REGULATED I-5111 PROTEIN PRECURSOR (IGUP I-5111).
245.34/31 (12%) 33367.4 / 6.98HUMANPOTASSIUM-TRANSPORTING ATPASE BETA CHAIN (EC 3.6.1.36) (PROTON PUMP) (GASTRIC H+/K+ ATPASE BETA SUBUNIT).
340.74/31 (12%) 49850.0 / 8.49MOUSEBETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N- ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.102) (CORE 2 BRANCHING ENZYME) (CORE2-GLCNAC-TRANSFERASE) (C2GNT).
4375/31 (16%) 54267.9 / 5.40MOUSEPERIPHERIN.
535.54/31 (12%) 22303.6 / 7.89MOUSEMETHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE (EC 2.1.1.63) (6-O- METHYLGUANINE-DNA METHYLTRANSFERASE).

Detailed Results


1. 8/31 matches (25%). 28723.3 Da, pI = 5.78. Acc. # Q06323. HUMAN. INTERFERON GAMMA UP-REGULATED I-5111 PROTEIN PRECURSOR (IGUP I-5111)..
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
840.5658840.5671-1.5624 110 116(K)(L)
971.4643971.4699-5.8174 191 198(K)(Q)
1268.67241268.652715.5113 25 35(K)(K)
1395.74551395.694236.7360 14 24(K)VDVFREDLCTK(T)1Cys-am
1395.74551395.7888-31.0076 221 232(R)(N)
1501.76531501.728724.4005 199 210(R)(D)
1517.89971517.857927.4997 59 72(K)(E)
1519.81561519.739350.2364 142 155(R)(V)
2286.21552286.049572.6162 90 109(K)KKGEDEDKGPPCGPVNCNEK(I)2Cys-am

23 unmatched masses: 602.1645 608.1908 644.2829 647.2125 650.0535 656.0550 666.0336 672.1385 694.1846 806.2445 825.1129 842.5013 955.3592 1045.5675 1109.5198 1449.8006 1475.8036 1484.7484 1941.0179 2211.2250 2233.2353 2285.2358 2384.0799

The matched peptides cover 43% (109/249 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


2. 4/31 matches (12%). 33367.4 Da, pI = 6.98. Acc. # P51164. HUMAN. POTASSIUM-TRANSPORTING ATPASE BETA CHAIN (EC 3.6.1.36) (PROTON PUMP) (GASTRIC H+/K+ ATPASE BETA SUBUNIT)..
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1045.56751045.543123.3903 189 198(K)(V)
1395.74551395.73855.0326 237 249(K)(L)
1517.89971517.796468.0449 79 92(R)(G)
2211.22502211.065072.3451 1 19(-)AALQEKKTCGQRMEEFQR(Y)1Met-ox Acet N

27 unmatched masses: 602.1645 608.1908 644.2829 647.2125 650.0535 656.0550 666.0336 672.1385 694.1846 806.2445 825.1129 840.5658 842.5013 955.3592 971.4643 1109.5198 1268.6724 1449.8006 1475.8036 1484.7484 1501.7653 1519.8156 1941.0179 2233.2353 2285.2358 2286.2155 2384.0799

The matched peptides cover 19% (56/291 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


3. 4/31 matches (12%). 49850.0 Da, pI = 8.49. Acc. # Q09324. MOUSE. BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N- ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.102) (CORE 2 BRANCHING ENZYME) (CORE2-GLCNAC-TRANSFERASE) (C2GNT)..
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1268.67241268.594561.3634 342 352(K)(F)
1941.01791940.915152.9624 142 156(R)AIYMPQNFYCIHVDR(K)1Cys-am
2211.22502211.196013.1250 99 116(R)DCASFIRTRKYIVEPLTK(E)1Cys-am
2384.07992384.1677-36.8406 193 211(R)VKADLNCMKDLYRMNANWK(Y)1Cys-am

27 unmatched masses: 602.1645 608.1908 644.2829 647.2125 650.0535 656.0550 666.0336 672.1385 694.1846 806.2445 825.1129 840.5658 842.5013 955.3592 971.4643 1045.5675 1109.5198 1395.7455 1449.8006 1475.8036 1484.7484 1501.7653 1517.8997 1519.8156 2233.2353 2285.2358 2286.2155

The matched peptides cover 14% (63/428 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


4. 5/31 matches (16%). 54267.9 Da, pI = 5.40. Acc. # P15331. MOUSE. PERIPHERIN..
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
971.4643971.443421.4506 462 469(K)(S)
1484.74841484.8477-66.8398 411 424(R)(T)
1941.01791940.936142.1699 305 320(R)(R)
2285.23582285.210111.2353 174 193(R)(R)
2384.07992384.2322-63.8962 187 205(R)(K)

26 unmatched masses: 602.1645 608.1908 644.2829 647.2125 650.0535 656.0550 666.0336 672.1385 694.1846 806.2445 825.1129 840.5658 842.5013 955.3592 1045.5675 1109.5198 1268.6724 1395.7455 1449.8006 1475.8036 1501.7653 1517.8997 1519.8156 2211.2250 2233.2353 2286.2155

The matched peptides cover 14% (70/475 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


5. 4/31 matches (12%). 22303.6 Da, pI = 7.89. Acc. # P26187. MOUSE. METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE (EC 2.1.1.63) (6-O- METHYLGUANINE-DNA METHYLTRANSFERASE)..
m/z
submitted
MH+
matched
Delta
ppm
startendPeptide Sequence
(Click for Fragment Ions)
Modifications
1045.56751045.6046-35.4647 187 196(K)(S)
1941.01791940.982518.2302 1 17(-)AETCKMKYSVLDSPLGK(M)1Cys-am
2286.21552286.2440-12.4633 129 150(K)(V)
2384.07992384.1734-39.2132 187 209(K)(N)

27 unmatched masses: 602.1645 608.1908 644.2829 647.2125 650.0535 656.0550 666.0336 672.1385 694.1846 806.2445 825.1129 840.5658 842.5013 955.3592 971.4643 1109.5198 1268.6724 1395.7455 1449.8006 1475.8036 1484.7484 1501.7653 1517.8997 1519.8156 2211.2250 2233.2353 2285.2358

The matched peptides cover 29% (62/210 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit 3.1.1, ProteinProspector 3.2.1
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